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Citations for New England Biolabs :
1 - 50 of 8932 citations for Medium Kit without Serum and without Growth Factors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... with or without DMSO (NEB), and the following protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins with or without PNGase F (NEB, USA) digestion were mixed with the loading buffer (250Mm Tris-HCl PH 6.8 ...
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bioRxiv - Genomics 2024Quote: ... Large (Klenow) fragment without exonuclease activity (exo-) (NEB) in combination with dATP or dGTP ...
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bioRxiv - Developmental Biology 2020Quote: ... Controls were handled with same protocol without λ-phosphatase (NEB). Phospho-mass spectrometry was performed as previously described (Nagai et al. ...
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bioRxiv - Microbiology 2020Quote: ... with or without prior digestion with DpnI (New England Biolabs), were analyzed on 0.8% agarose gel followed by Southern blotting using a 32P-labeled DNA probe specific for AAV2 rep (for detection of wtAAV2) ...
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bioRxiv - Molecular Biology 2022Quote: ... with or without addition of murine RNase inhibitor (RI, NEB). Intracellular tRNA-derived fragments were generated by exposing cells to 500 µM sodium arsenite (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries were prepared without rRNA depletion using NEBNext Ultra II Directional RNA Library kit (NEB) with the following modifications ...
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bioRxiv - Genetics 2020Quote: ... without modification except for library preparation performed with NEBNext® Ultra kit (New England Biolabs®). For library preparation 600 ng of each genomic DNA were fragmented by sonication and purified to yield fragments of 150-200 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted DNA was incubated with or without RNase H (New England Biolabs) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... or without 350 units of recombinant GSK3β (rabbit skeletal muscle) (New England BioLabs) and 1X hot kinase buffer (50mM Tris ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mM dithiothreitol) with or without 50 µM ATP (P0756S, New England Biolabs) at 30°C for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 15μL of myc-eIF6-bound beads were incubated with/without recombinant GSK3β (NEB) and suspended in cold kinase buffer and incubated at 30°C for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: ... The samples were mixed with 6 × purple loading dye without sodium dodecyl sulfate (NEB) and with 10 × TBE to make a 1 × solution ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1% BSA) with or without Short Cut RNase III (New England Biolabs; 1:150) for 90 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... Association occurred in samples containing ACE2- fusion proteins either without or with TEV protease (NEB) treatment ...
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bioRxiv - Bioengineering 2020Quote: ... we use Western Blot to detect spike protein with/without treatment of PNGase F (NEB). 100 ng particles were incubated with Glycoprotein Denaturing Buffer at 98°C for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were mixed with a 6X loading buffer without SDS and reducing agents (BioLabs, B7025S) and without boiling loaded onto Native-PAGE gels (NativePAGE ...
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bioRxiv - Microbiology 2022Quote: ... 100 ng of DNA was treated with or without 6.6 units of ExoV (RecBCD, NEB) in a 10 µl reaction volume and incubated for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and equivalent amounts of Hirt DNA were digested with or without DpnI (New England Biolabs) and analyzed on a 0.8% neutral agarose gel followed by transfer to a nitrocellulose membrane ...
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bioRxiv - Evolutionary Biology 2023Quote: ... g165 and g166 without the predicted signal peptides were PCR-amplified with Phusion polymerase (NEB) from genomic DNA of P ...
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bioRxiv - Microbiology 2021Quote: ... The first EcoRI restriction site at 829 bp within the AgeI and KasI restriction sites in RGD4C-AAVP-TNF was deleted in two steps to mutate a thymidine to cytosine nucleotide at position 833 without altering the translated amino acid using the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) by following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... SCAR-seq libraries were prepared following the previous published protocol (11) without any modification except using NEBNext® Ultra™ II DNA library prep kit (NEB) for the end repair ...
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bioRxiv - Cell Biology 2022Quote: ... cleared lysate was incubated without or with PNGase F (NEB, 1 μl per 40 μl lysate) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... a master mix was prepared containing 2 µl 10x T4 polynucleotide kinase buffer without ATP (NEB) and 1 µl murine RNase inhibitor per sample and 3 µl were added to each sample together with 2 µl truncated T4 polynucleotide kinase (#M0201 ...
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bioRxiv - Cell Biology 2019Quote: ... Excess bead primers without RNA molecules were removed by the treatment of Exonuclease I (NEB, NEBM0293S). Then ...
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bioRxiv - Genomics 2024Quote: ... and pooled into 7 ml ligation buffer (1X ligation buffer 3 (New England Biolabs; without ATP), 1 mM ATP ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were labelled by replacing complete growth medium with 500 μl serum free DMEM/F12 containing 200 nM SNAP Surface Alexa Fluor-488 (New England Biolabs) and were incubated for 30 min at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: Recombinant condensin II holo(WT) at 500 nM was mixed with or without λ protein phosphatase (NEB) at a final concentration of 400 U/µL in 1x NEBuffer for Protein MetalloPhosphatases supplemented with 1 mM MnCl2 and incubated at 30°C for 60 min ...
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bioRxiv - Biochemistry 2021Quote: ... with and without the addition of ACE2 (~1:1.25 S-protein trimer:ACE2 molar ratio) (New England Biolabs). Vitrified samples of S-protein constructs with and without ACE2 were prepared by first glow discharging Quantifoil R1.2/1.3 300 mesh holey carbon copper grids for 1 minute using a Pelco easiGlow glow discharge unit (Ted Pella ...
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bioRxiv - Biochemistry 2021Quote: ... the denatured samples were incubated at 37 °C with or without Endo Hf (P0703S, New England Biolabs) or PNGase F (P0704S ...
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bioRxiv - Molecular Biology 2023Quote: The coding sequence of PolA without the start codon was amplified by PCR using Q5 polymerase (NEB) and cloned BamHI-XhoI into pET28a (Thermo ...
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bioRxiv - Biochemistry 2023Quote: ... SUMO21-92 and ISG1579-156 without tag and subsequently cloned into the pTXB1 vector (New England Biolabs) by restriction cloning according to the manufacturers protocol.
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bioRxiv - Molecular Biology 2022Quote: ... Two 100 μl aliquots were incubated overnight at 37°C with and without Endo H (3000 U, NEB). Then the samples were heated with 100 μl SDS sample buffer for 5 min at 95°C.
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bioRxiv - Cell Biology 2022Quote: ... with or without phosphatase addition (0.25 μL of 11 phosphatase in 50 μL reaction volume, New England Biolabs, P0753 or 200 nM purified 6xHis-Calcineurin ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 million permeabilized cells (without any antibodies bound) were incubated with 4 units of Dam enzyme (NEB, M0222L) during the activation step ...
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bioRxiv - Cancer Biology 2023Quote: ... RNAs that were amplified without a poly-A tail were poly-adenylated using poly-A polymerase (NEB M0276) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... plasmid backbones without eYFP were amplified by PCR with PR41 and PR42 using Q5 Polymerase (New England Biolabs) and pAJM657 ...
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bioRxiv - Cancer Biology 2024Quote: ... Dot blot samples were prepared from 1 µg gDNA with or without RNaseH1 2500 U treatment (NEB, M0297) O/N 37°C.Samples were denatured in Denaturation Buffer (NaOH 400 mM ...
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bioRxiv - Molecular Biology 2021Quote: Purified genomic DNA was processed with (+) or without (−) an enzymatic cocktail composed of AAG (New England BioLabs, cat# M0313S) and APE1 (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: ... lectin (70 to 1599-bp CDS of ACA1_164810) without their signal sequences into pMAL-p2x vector (New England Biolabs) for periplasmic expression in BL21-CodonPlus(DE3)-RIPL (Agilent Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... 2 ul of cDNA without dilution was used for each PCR reaction by Phusion® HighFidelity DNA Polymerase (NEB) for 35 cycles ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μg of supercoiled plasmid DNA was incubated at 37 °C for 3 hours with purified SpRY protein at a final concentration of 1 μM and IVT gRNA (prepared without DNase treatment) at a final concentration of 2 μM in Buffer 3.1 (NEB). Reactions were stopped by the addition of 1 μL of Proteinase K (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... we incubated the Pol II EC(CPD) (with and without biotin crosslinking) with 2μl streptavidin magnetic beads (NEB, USA) for 30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: To generate circular DNA with a single nick phagemid DNA (~2,6 μg) with or without non-denaturing bisulfite treatment was treated with 20 Units of Nt.BstNBI (New England Biolabs) for 1 hour at 37°C in 50 μl of 1X NEBuffer 3.1 followed by deactivation at 70°C for twenty minutes.
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bioRxiv - Genetics 2020Quote: The long fractions (with and without rRNA depletion) were further processed with the NEBNext Magnesium RNA Fragmentation Module (NEB), as described in the manual ...
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bioRxiv - Genomics 2022Quote: ... then washed once with 5ml fix buffer (without formaldehyde) and once with 1.25 x NEB3 buffer (New England Biolabs), with centrifugation for 5 min at 4500g ...
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bioRxiv - Neuroscience 2022Quote: Proteins from neuronal culture at DIV 14 were extracted as described above and treated with and without N-glycanase or endoglycosidase H according to manufacturer’s instructions (New England Biolabs). The lysates were analyzed with Western blot using LAMP1 (1:4000 ...
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bioRxiv - Cell Biology 2023Quote: ... Total lysates (200 µg each) were incubated with or without 400 U lambda protein phosphatase (New England Biolabs, #P0753S) and 1 mM MnCl2 at 30 °C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... The ORF of TAP110 amplified and inserted without the stop codon by cloning with the restriction enzymes HindIII HF and XhoI (NEB) into a modified pLew100 vector for overexpression [42,52] ...
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bioRxiv - Genetics 2020Quote: ... was incubated with or without 5 µM nucleotides DNA substrates (cssDNA, Phi X174; or ldsDNA, Phi 174 RF I; both from NEB) with 1 mM ATP in ATPase buffer (25 mM Tris-HCl [pH 7.5] ...