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Citations for New England Biolabs :
351 - 400 of 2688 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... an antisense template DNA for the neural crest marker sox846 was generated by linearising with EcoRI (New England Biolabs). Then a digoxigenin-labeled probe against sox846 was transcribed in vitro by using this linearised plasmid as a template and by following the instructions of a commercial in vitro Transcription System (Promega P1420) ...
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bioRxiv - Neuroscience 2019Quote: ... As a marker 70 µl of Color Protein Standard Broad Range (New England BioLabs, #P7712S; 25 to 245 kD) diluted 1/7 in SDS sample buffer was loaded ...
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bioRxiv - Microbiology 2022Quote: ... and selection marker of pSIN was replaced to match that of pMCB306 using the Gibson Assembly Cloning Kit (NEB) to generate the transfer plasmid (BAS2186 ...
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bioRxiv - Microbiology 2020Quote: ... including equimolar amounts of dGTP and 7-deaza-GTP (New England Biolabs), at a concentration of 200 µM was used (Maertzdorf et al. ...
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bioRxiv - Biophysics 2022Quote: ... rk430-mScarlet-SNAP (7 μM monomer) was incubated with benzylguanine-biotin (NEB) in a 4 to 1 molar ratio at room temperature for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 7 μl PEG8000 and 1 μl T4 RNA ligase 2 K227Q (NEB) at 25 °C for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... 7 μg pNZdmsC3GH plasmid was digested with 40 U of SfiI (NEB), separated on a 1% agarose gel and ...
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bioRxiv - Microbiology 2022Quote: ... for 7-12 h at 37°C in CutSmart buffer (NEB, B7204S). Digested products were then visualised on a 4% NuSieve (Lonza ...
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bioRxiv - Microbiology 2019Quote: ... and 30 ng/ul Proteinase K (NEB)) ...
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bioRxiv - Bioengineering 2023Quote: ... and 60 ng/µL Proteinase K (NEB)) ...
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bioRxiv - Neuroscience 2019Quote: ... excess gel was removed and embedded specimen were placed in digestion buffer (1X TAE, 0.5% Triton X-100, 0.8 M guanide HCL) with 8 units/ml Proteinase K (NEB) for 2 hours at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.5μL of 20% Triton X-100, 1μL BSA (100x, 10mg/mL) and 10μL HaeIII (10U/μL, NEB, R0108S) with shaking at 37°C overnight ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... resuspended purified nuclei were incubated with 1 mM CaCl2 and 100 ku/ml micrococcal nuclease (New England Biolabs) in HB for 37°C for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... cells were harvested 3 days after electroporation and genomic DNA was extracted with 100 μL lysis buffer (10 mM Tris-HCl, pH 7.5, 0.05% SDS, 25 μg/mL proteinase K (NEB)) at 37 °C with incubation for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 ng for CTCF and 10 ng for input) were processed using NEBNext ChIP-seq library (New England Biolabs) with manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... we combined 50 ng of backbone with 25 ng of annealed oligo along with T4 ligase buffer (NEB #B0202S), T4 ligase (#M0202S) ...
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bioRxiv - Bioengineering 2023Quote: ... we combined 50 ng of backbone with ∼25 ng of annealed oligo along with T4 ligase buffer (NEB #B0202S), T4 ligase (#M0202S) ...
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bioRxiv - Developmental Biology 2023Quote: ... along with 2.5 ng/μl pCFJ90(Pmyo-2::mCherry) and 77.5 ng/μl of 2-Log DNA ladder (New England BioLabs), to generate two independent strains expressing Ex[Pcol-10::mCherry::his-58::SL2::drl-1 cDNA] (DLS515 ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 100 ng digested pMB1-10x and 2.4 ng amplified sgRNA library insert were assembled using Gibson Assembly Master Mix (NEB) in a 20 μL reaction for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... The integrity of extracted DNA was confirmed by agarose gel electrophoresis by loading equal amount of extracted DNA on agarose gel along with גHindIII digest marker (NEB) 27 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... was used to determine the position and the strength of the radioactive signal in each of the lanes compared with the molecular marker (Quick-Load1 kb DNA Extend DNA Ladder; New England BioLabs) to calculate telomere lengths for each sample ...
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bioRxiv - Synthetic Biology 2021Quote: ... were amplified from pSerOTSλ and placed under the control of the glnS* promoter and assembled with the kanamycin selection marker and origin of replication from pSerOTSλ by gibson assembly using NEBuilder HiFi DNA Assembly master mix (NEB). tRNA expression cassettes (1x- ...
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bioRxiv - Neuroscience 2020Quote: ... a gel slice corresponding to 60 to 80 nt was isolated (based on size markers in an adjacent lane: low range ssRNA ladder, NEB). The reverse transcribed product in the gel slice was eluted ...
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bioRxiv - Neuroscience 2020Quote: ... a gel slice corresponding to 80 to 150 nt was isolated (based on size markers in an adjacent lane: low range ssRNA ladder, NEB). The reverse transcribed product in the gel slice was eluted ...
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bioRxiv - Microbiology 2021Quote: ... were cloned into the pEX18.AP vector (with ampicillin resistance as a selectable marker) using the Gibson Assembly Cloning Kit (New England Biolabs) per the manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: ... Each gel also contained 32P-end-labeled size ladders (Molecular Weigh Marker XV, by Roche Diagnostics and 1Kb DNA ladder by New England Biolabs). After electrophoresis ...
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bioRxiv - Developmental Biology 2020Quote: ... 300ng of undigested gDNA and from each restriction reaction were loaded in a 1% agarose gel with molecular weight marker (N3232, NEB). 160V current was applied for 30min in a Midigel tank (370000 ...
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bioRxiv - Molecular Biology 2020Quote: ... the plasmid harboring the trf1 disruption cassette with the HYGR marker (named pUTrf1-Hyg) was constructed through Gibson Assembly (NEB) of four overlapping PCR fragments ...
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bioRxiv - Plant Biology 2021Quote: ... Selected SNPs were converted to restriction enzyme-based Cleaved Amplified Polymorphic Sequence (CAPS) markers (Supplementary Table 1) using Neb cutter v2.0 (New England Biolabs Inc). Restriction digestion was performed according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2019Quote: A construct expressing SURF4 and the Katushka2S fluorescent marker (PGK-SURF4-p2A-Katushka2S) was assembled with the NEBuilder HiFi DNA assembly cloning kit (NEB) using vector sequence derived from LV1-5 (Addgene #68411 ...
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bioRxiv - Microbiology 2021Quote: ... cassette was inserted between the VP30 gene and the blasticidin resistance marker by restriction enzyme digestion of the vector with SpeI-HF and SalI-HF (NEB), and digestion of a pEF1a-IRES vector (www.takarabio.com ...
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bioRxiv - Genetics 2022Quote: ... and cloned upstream of the TRP2 terminator and NatMX6 drug marker cassette in a pUC19-based vector using the NEB HiFi Assembly kit (New England Biolabs). This AB614_yOTC plasmid (GenBank ...
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bioRxiv - Evolutionary Biology 2022Quote: ... melanogaster strain to be injected and inserted into plasmids containing fluorescent eye markers using NEBuilder Hi-Fi DNA assembly (NEB). See key resources table for primers sequences and donor plasmids.
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bioRxiv - Cell Biology 2023Quote: ... Linearization of auxotrophic marker integration plasmids by treatment of plasmids pOS7 and pOS642 with the restriction enzymes StuI and BstII (NEB) were transformed for generation of ORS1150 and ORS1787 ...
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bioRxiv - Systems Biology 2023Quote: ... the library pool ranging from 135 to 146 bp including the DNA marker were gel purified and quantified by NEBNext Library Quant kit (NEB) using QuantStudio 5 Real-Time PCR System (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... 30 ng pT-plasmid either carrying the blasticidin or the puromycin resistance marker and 1 unit HIFI Polymerase (Q5 HF DNA Polymerase, M04915, NEB) and 1× HiFi reaction buffer (05917131103 ...
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bioRxiv - Biochemistry 2023Quote: ... The pCR8[mZC3H18] plasmid was used as a template to generate subsequent ZC3H18 cDNA constructs that were cloned into a piggyBAC (pB) vector containing an N-terminal MYC tag and BSD selection marker using NEBbuilder HiFI DNA assembly (NEB). OsTIR1-HA ...
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bioRxiv - Cell Biology 2022Quote: ... Phosphorylation reaction containing TEX264-FLAG and CK2 (mixture of CK2A1 and CK2B, P6010, NEB) with or without 1 mM CX4549 (S2248 ...
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bioRxiv - Bioengineering 2020Quote: ... 2 μL of PCR mixture was used for 10 μL KLD reaction (NEB, MA), which proceeded under room temperature for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... the RT reaction mixture was diluted 40x into a HotStart Taq (New England Biolabs) PCR reaction (1 mM MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... The template vector was removed from the PCR mixture by DpnI treatment (NEB, USA) at 37°C (1 – 2 h ...
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bioRxiv - Plant Biology 2020Quote: ... 19 μL binding mixture containing 4 μL nuclear extracts and 5U Rnase H (NEB) or bovine pancreatic RNase A (Qiagen ...
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bioRxiv - Biophysics 2022Quote: ... The reaction mixture for polymerization contained Hot-start Taq DNA polymerase (New England Biolabs), Taq standard reaction buffer ...
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bioRxiv - Genomics 2021Quote: ... the ligation mixture (2.0 μl 10x RNA Ligase Buffer [with DTT; New England Biolabs] ...
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bioRxiv - Molecular Biology 2021Quote: The DNA-complex-coated beads were incubated with the end-repair enzyme mixture (NEB) at 20 °C for 30 min to repair the DNA ends and then with Klenow exo- (NEB ...
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bioRxiv - Genomics 2022Quote: ... An isothermal extension reaction mixture was made from 35μl NEBuffer2 (10×, New England Biolabs), 12.5μl Klenow Fragment (5,000U/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The enrichment PCR master mixture contained 2,200 OneTaq 2X Master Mix (New England Biolabs), 16.5 uL synHEK3 P7 enrichment primer (100 uM) ...
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bioRxiv - Immunology 2023Quote: ... with a mixture of 0.33 µM EnGen Cas9 protein (New England Biolabs, Cat.No. M0646T), two Cas9 guides RNA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Injection mixtures contained 10μl restriction digest including 5 units of I-SceI enzyme (NEB), 1μl CutSmart buffer ...
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bioRxiv - Biophysics 2023Quote: ... the mixture was purified using the Monarch PCR and plasmid DNA purification kit (NEB), eluted in 10 mM Tris-HCl pH8.0 and stored at -20 °C for further experiments ...