Labshake search
Citations for New England Biolabs :
551 - 600 of 2688 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... RNA pellets were resuspended in 5 µL dephosphorylation reaction mix (7 mM Tris pH 8, 1x NEB T4 PNK buffer ...
-
bioRxiv - Developmental Biology 2021Quote: ... and elongation for 7 min at 72°C) using the Phusion High-Fidelity DNA Polymerase (NEB, #M0530S) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... and elongation for 7 min at 72°C) using the Phusion High-Fidelity DNA Polymerase (NEB, #M0530S) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... 1 µg of DNA was mixed with 7 µl NEBNext Ultra II end prep reaction buffer (NEB) and 3 µl Ultra II end prep enzyme mix (NEB) ...
-
bioRxiv - Microbiology 2021Quote: ... 500 μl of lysed cells were transferred to a tube containing 4.5 ml of digestion mix (1X NEB 3 buffer, 1% triton X-100) and 100 μl of the lysed cells were transferred to a tube containing 0.9 ml of digestion mix ...
-
bioRxiv - Zoology 2021Quote: ... The PCR mixtures for first step and nested PCR step (12.5 μl) contained 1X OneTaq Hot Start Master Mix (NEB) and 0.2 μM of each primer ...
-
bioRxiv - Pathology 2019Quote: ... by adding a mixture of 10 µl DNase I and 190 µl DNase I Reaction Buffer (New England Biolabs) to the priming port and incubated for 30 minutes at room temperature ...
-
bioRxiv - Genetics 2020Quote: ... and resuspended in 750 µl ligation mixture containing 200 units T4 DNA ligase (NEB/ IMB core facility protein production), 1X T4-Ligation buffer (NEB ...
-
bioRxiv - Genetics 2020Quote: ... A 6 µL mixture containing 2 µL of 40 µM Spy Cas9 NLS protein (New England Biolabs, MA, USA), 200 ng each of five sgRNAs (in 2 µL ...
-
bioRxiv - Biochemistry 2021Quote: ... The mixture was incubated at room temperature for 10 min followed by transformation into 5-alpha competent cells (NEB). The E ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 5 µL of the ligation mixture was added to 50 µL of chemically competent NEB10β cells (New England Biolabs) and transformation was performed following manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... the first step PCR reaction was performed in a 12.5 μl mixture containing 1X OneTaq Hot Start Master Mix (NEB), 0.4 μM of each DHPV-U 1538F and DHPV-U 1887R primer and 20 ng of DNA template ...
-
bioRxiv - Genomics 2019Quote: ... and the mixture was incubated at 45°C (for Hybridase RNase H) or 22°C (for NEB RNase H) for additional 5 minutes ...
-
bioRxiv - Biochemistry 2022Quote: ... The beads were covered with 30 μL of reverse transcription mixture containing dNTPs (0.5 mM each, New England BioLabs), acetylated BSA (1,5 μg) ...
-
bioRxiv - Plant Biology 2022Quote: ... 2.5 μl of A-Tailing control and 12.5 μl of A-Tailing mixture (New England Biolabs, Ipswich, MA, USA) was added to the DNA solution in a final volume of 30 μl and vortexed at 1,800 × g for 2 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... a mixture of 0.5 μg of IMAC purified protein and 0.5 μg BSA (New England Biolabs, Ipswich, MA, USA) in 100 μl of coating buffer per well was added to 384-well microtiter plates (1 μg without BSA for direct detection) ...
-
bioRxiv - Molecular Biology 2023Quote: ... A PCR master mixture was prepared by combining 2,200 uL 2x NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs), 22 uL common P7 primer (100 uM ...
-
bioRxiv - Neuroscience 2023Quote: ... DNA ligation was performed in the same reaction mixture containing the phosphorylated samples using 5U T4 DNA ligase (NEB). A transformation assay was performed using 3 μL DNA ligation product in DH5alpha bacterial cells and the positive clones were verified by Sanger sequencing.
-
bioRxiv - Biochemistry 2023Quote: 3 μL of the transcription and co-transcriptional reactions were added to a 6 μL mixture containing 1x DNase I reaction buffer and 0.5 U/μL DNase I (New England Biolabs). After incubation at 37°C for 30 min ...
-
bioRxiv - Plant Biology 2019Quote: ... the 100 bp ladder (NEB) was used ...
-
bioRxiv - Genetics 2020Quote: ... and 100 U CviAII (NEB), and diluted to 500 μl ...
-
bioRxiv - Biophysics 2019Quote: ... 100 units EcoRV-HF (NEB) was added and left 8-10 h at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... 100 mM SAM (NEB, B9003S), and 5 μM recombinant Trm1 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 100 bp (NEB, N3231) DNA ladders ...
-
bioRxiv - Microbiology 2019Quote: ... Primer sequences were as previously described.7 Samples were digested with 1 unit of BamHI-HF enzyme (NEB) prior to running the PCR ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... End repair was performed by adding 20 μl of the End Repair Mix (7 μl of 10x NEB ligation buffer ...
-
bioRxiv - Genomics 2023Quote: ... Gaps in the transposed DNA were filled by 15μL gap-fill mix (7 μL Q5 reaction buffer (NEB), 7 μL Q5 high GC enhancer (NEB) ...
-
bioRxiv - Genomics 2019Quote: 500 ng of genomic DNA was enzymatically fragmented by dsDNA Fragmentase (NEB). The library was prepared using the NEBNext Ultra II kit and indexes from the Dual Index Primers Set 2 (all New England Biolabs ...
-
bioRxiv - Molecular Biology 2021Quote: ... 20 ng genomic DNA was either treated with exonuclease V (RecBCD, NEB), in a total volume of 30 ul ...
-
bioRxiv - Molecular Biology 2021Quote: ... 20 ng genomic DNA was either treated with exonuclease V (RecBCD, NEB), in a total volume of 30 μl ...
-
bioRxiv - Genomics 2021Quote: ... NGS libraries were prepared using NEB DNA Ultra II kit (NEB#E7645). End prep ...
-
bioRxiv - Plant Biology 2021Quote: ... 50 ng of gDNA was digested in NEBuffer2 with McrBC (NEB, M0272S) at 37°C for 4 h and inactivated at 65°C for 30 min ...
-
bioRxiv - Microbiology 2020Quote: ... 1.25 µl of 20,000 ng/µl bovine serum albumin (New England Biolabs), and 7.125 µl of molecular grade water ...
-
bioRxiv - Genetics 2021Quote: ... and 500 ng of DNA were digested either with HpaII/EcoRI (NEB) (Tube A ...
-
bioRxiv - Neuroscience 2022Quote: ... 10 ng genomic DNA was digested with 30 units of MspI (NEB). Fragments were ligated to pre-annealed adapters containing 5’-methyl-cytosine instead of cytosine according to Illumina’s specified guidelines ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 ng genomic DNA was digested with HaeIII (New England Biolabs, R0108L). TBP served as the internal control ...
-
bioRxiv - Genetics 2024Quote: ... The DNA (500 ng) was incubated with DNase I (1U, NEB, M0303L), Fast AP (1 U ...
-
bioRxiv - Genomics 2021Quote: ... along with 100 units of MscI and 100 units of AseI (NEB #R0534M and #R0526M) to a total volume of 370 μl in a 1.5 ml lo-bind Eppendorf tube ...
-
bioRxiv - Genomics 2020Quote: ... and cloned into 100ng of BbsI linearized pKLV2-U6gRNA(BbsI)-ccdB-PGKpuro2ABFP-W vector by Gibson Assembly Cloning (New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... and 200 μM each of dATP/dCTP/dTTP at 21°C for 10 min and an additional 15 min incubation with 25 nM recombinant human RPA (a gift from Sarah W. Cai) and 0.03 U/μl T4 DNA polymerase (NEB, #M0203) at 37°C ...
-
bioRxiv - Biophysics 2021Quote: ... was filled in with a mixture of natural and biotinylated nucleotides by the exonuclease-deficient DNA polymerase I Klenow fragment (New England BioLabs). Reaction was conducted by incubating 10 nM λ-DNA ...
-
bioRxiv - Cell Biology 2020Quote: ... Quantification of mRNA level was performed in a 20 μl mixture consisting of 10 μl Q5 High-Fidelity 2X Master Mix (NEB), 0.2 μl RT-PCR product ...
-
bioRxiv - Cell Biology 2022Quote: ... an enzyme mixture of 3 μl in volume that contains 0.01 U Bst 2.0 DNA polymerase (New England Biolabs, United States), 0.5 U SplintR ligase diluted with Diluent A (New England Biolabs ...
-
bioRxiv - Biochemistry 2020Quote: ... The reaction mixture was then dried completely and resuspended in a mixture containing 50 mM ammonium bicarbonate and PNGase F (New England Biolabs) using only H2O18 (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2019Quote: ... Second-strand cDNA was synthesized in a reaction mixture containing Second-Strand Synthesis Kit (New England Biolabs; Ipswich, MA, USA). cDNA was purified using the Qiagen purification kit ...
-
bioRxiv - Biochemistry 2021Quote: ... Each well of the 96-well destination plate received the following Golden Gate reaction mixture: 0.5 µL of T7 DNA Ligase (NEB; M0318S), 0.5 µL of AarI (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2020Quote: ... through a Gibson Assembly reaction using an in-house Gibson mixture (gifted by the Solnica-Krezel lab, Washington University in St. Louis; NEB Phusion ...
-
bioRxiv - Genomics 2020Quote: ... The ligation mixture was discarded and the crosslink reversal was performed by adding 50 μL 1X CutSmart buffer (New England Biolabs) and 20 μg Proteinase K (Qiagen ...
-
bioRxiv - Biochemistry 2021Quote: ... The floated fraction corresponding to a mixture of high- and low-density EVs (42) was treated with MNase (NEB, M0247S) to degrade any nucleic acid not contained within the EVs and deactivated with 25 mM ethylene glycol-bis(β-aminoethyl either)-N,N,N′,N′-tetraacetic acid (EGTA ...
-
bioRxiv - Synthetic Biology 2021Quote: ... the resulting DNA mixture could be directly used for following the ligation with the corresponding linear capture vectors by T4 DNA ligase (NEB).