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Citations for New England Biolabs :
51 - 100 of 299 citations for Inactive Serine Protease 35 PRSS35 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Re-joined ends were amplified by 35 cycles of PCR from 0.8 µl template using Q5 High-Fidelity DNA Polymerase (New England Biolabs) and primers NOSprom-nested-F2 and TagRFP-nested-R2 (Table S1 ...
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bioRxiv - Microbiology 2024Quote: ... the sequence encoding amino acid residues 1 to 35 of the N-terminal end of BVG96_RS17270 (89) was PCR amplified using Q5 polymerase (NEB) and cloned via NEBuilder HiFi DNA Assembly (NEB ...
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bioRxiv - Biochemistry 2019Quote: ... and 3 protease inhibitor cocktail tablets (New England Biolabs) were added to the cell suspension ...
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bioRxiv - Molecular Biology 2019Quote: ... 500 nL of protease mix was added (1x NEB CutSmart buffer ...
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bioRxiv - Cell Biology 2021Quote: ... samples were digested by protease K (New England Biolabs), eluted and cross-link-reversed ...
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bioRxiv - Genomics 2021Quote: ... 250 nL of protease mix was added (1× NEB CutSmart buffer ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of TEV protease (New England Biolabs, #P8112S) was added ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were cleaved with TEV protease (New England Biolabs) and further purified via reverse IMAC ...
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bioRxiv - Microbiology 2021Quote: The chimeric CrPV-aNCV infectious clone was derived from the full-length CrPV infectious clone (pCrPV-3; Accession KP974707) (35) using Gibson assembly (New England BioLabs), per the manufacturer's instructions ...
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bioRxiv - Plant Biology 2019Quote: ... and cloned either alone or under of the 35S promoter together with GFP and mScarlet-i into pPLV03 or pGEX5x3 using Gibson Assembly (NEB). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: Deglycosylation of S protein from the lysate of HEK293 cells transfected with pTwist-EF1a-nCoV-2019-S-2xStrep plasmid was performed as described previously [35] using PNGase F (New England Biolabs).
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bioRxiv - Cell Biology 2021Quote: ... coding sequence was amplified from Arabidopsis cDNA and assembled into a pGreen0179-35S-spFLA11-His-YFP vector using NEBuilder HiFi DNA Assembly kit (NEW ENGLAND Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... A volume of cDNA corresponding to 2.5 ng of the input RNA was subjected to 35 cycles of PCR with the 2 x NEBNext Ultra II Q5 Master Mix (NEB).
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bioRxiv - Synthetic Biology 2019Quote: ... two oligonucleotides were designed with an overlap of 35 bp containing the T7 promoter sequence and amplified using Q5 High-Fidelity DNA polymerase (NEB) according to the suppliers instructions ...
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bioRxiv - Biochemistry 2019Quote: ... expression constructs containing WT or mutant full-length human KAP1 preceded by a triple FLAG tag and linker sequence MDYKDHDGDYKDHDIDYKDDDDKGSGG were assembled in pLEXm (35) with the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). The following plasmids were used ...
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bioRxiv - Synthetic Biology 2021Quote: ... [35] obtained from Benenson lab at ETH Zurich was digested with 30 U of SalI-HF (New England Biolabs (NEB), R3138S ...
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bioRxiv - Genetics 2020Quote: ... The purified DNA fragment (40 ng) was ligated into pICSLUS0003OD (35S USER vector) with the USER enzyme (New England Biolabs). After verifying the presence of the insert by colony PCR ...
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bioRxiv - Genetics 2019Quote: ... The affl-2 driver strain was constructed by injecting 25 ng/µl of pSJW003 along with 40 ng/µl of Punc-122::rfp and 35 ng/µl of 1 kb DNA ladder (NEB) into the GFP effector strain syIs300 ...
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bioRxiv - Plant Biology 2022Quote: All ObANS genes were inserted into the pBI121 plasmid under the control of the constitutive Cauliflower mosaic virus 35S promoter using the SacI and XbaI restriction enzymes (Thermo-scientific) and the NEBuilder ligation system (NEB). The plasmids were transformed into Agrobacterium tumefaciens strain GV3101 using electrotransformation and positive colonies were selected with kanamycin ...
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bioRxiv - Plant Biology 2023Quote: ... and enhancer fragment combinations were cloned upstream of the 35S minimal promoter by Golden Gate cloning using BsaI-HFv2 (NEB). The resulting libraries were bottlenecked to yield 10–20 barcodes per enhancer.
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bioRxiv - Genomics 2023Quote: ... was cloned between residues 34 and 35 with primers ag424 and ag425 using inverse PCR with Q5 polymerase (New England Biolabs, NEB).23 The sequences of all primers used in this study are presented in Table S2 ...
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bioRxiv - Plant Biology 2023Quote: ... and rbcS-E9 enhancers and the synthetic enhancers syn1–11 were cloned upstream of the 35S minimal promoter in pDL by Golden Gate cloning using BsaI-HFv2 (NEB). The synthetic enhancers were ordered as synthesized DNA fragments.
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bioRxiv - Microbiology 2021Quote: F(ab’)2 fragments were generated using IdeZ protease (NEB), purified using CaptureSelect LC-lambda affinity matrix (human ...
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bioRxiv - Biophysics 2021Quote: ... The desalted sample was then incubated with TEV protease (NEB) (50 units ...
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bioRxiv - Microbiology 2020Quote: ... the resin was treated with factor Xa protease (P8010L, NEB) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... 1X protease inhibitor cocktail ± lambda protein phosphatase (New England Biolabs) as per the manufacturer’s instructions and incubated at 30°C for one hour.
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bioRxiv - Neuroscience 2020Quote: TEV protease (TEV-P) was purchased from NEB (Cat#P8112S). PFFs were prepared as above and premixed 1:10 (for a final concentration of 15 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... with the addition of 1 µL GluC protease (P8100S, NEB) at 1 mg/mL per mg of RH5ΔNL (1:1000 wt/wt) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Bi-directional precursor construct (pZ073) [35] obtained from Benenson lab at ETH Zurich was digested with 30 U of SalI-HF (New England Biolabs (NEB), R3138S ...
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bioRxiv - Genomics 2022Quote: ... 1500 calcein-stained cells in 5 μL of were added to 35 μL of barcoded hydrogel templates with 29 U/mL Proteinase K (NEB #P8107S) and 70 mM DTT (Sigma #D9779 ...
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bioRxiv - Molecular Biology 2023Quote: ... full length CDS of TaSnRK1α gene was amplified using the wheat cDNA and cloned into the pCAMBIA1302 vector under the constitutive promoter 35S using the Gibson assembly cloning kit (Gibson Assembly® Master Mix, NEB) with the help of restriction enzyme NcoI ...
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bioRxiv - Genomics 2023Quote: ... was cloned between residues 34 and 35 with primers ag424 and ag425 using inverse PCR with Q5 polymerase (New England Biolabs, NEB).23 The sequences of all primers used in this study are presented in Table S2 ...
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bioRxiv - Plant Biology 2023Quote: The coding region of NIa-Pro protein was cloned from TuMV into the binary vector pMDC32 downstream of the 35S promoter as well in pSITE vector using Gibson assembly (New England Biolabs, USA)(Gibson et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... The rbpms2bsa9329 surrounding genomic region was amplified for 35 cycles with an annealing temperature of 60°C and the mutant allele was digested with MboII (New England Biolabs, R0148S)2 or the wild-type allele was digested with HphI (New England Biolabs ...
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bioRxiv - Physiology 2021Quote: ... Full-length cDNA sequences were obtained in 35 cycles of PCR reactions with Phusion DNA polymerase (New England Biolabs, Ipswich, USA; MO531L) and specific primers designed against the sequence of the phylogenetically characterized white seabass β-NHE obtained from the combined gill and RBC transcriptome (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... full length CDS of TaSnRK1α was cloned into plant expression vector pCAMBIA1302 under the constitutive promoter CaMV:35S with the help of restriction site NcoI on both ends using Gibson assembly® master mix (NEB, USA). Primer details are provided in Supplementary Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... These solutions were added to 100 units of TEV protease (NEB) in aggregation buffer ...
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bioRxiv - Immunology 2020Quote: ... 0.09% Tween-20) containing 0.2 mg/ml protease K (NEB, P8107S) and incubating at 56°C for 1 hour ...
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CHD7 interacts with the nucleosome acidic patch for its efficient activity via its N-terminal regionbioRxiv - Biochemistry 2020Quote: ... Samples were deproteinized with 0.5 mg/ml of Protease K (NEB) and incubated for 1 hrs at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... 1/20-1/50 (w/w) GluC protease (New England BioLabs) was added and incubated 24-48 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The ubiquitin (in vivo) and 3xFlag-TEV (using TEV protease, NEB) fusions were removed to reveal three N-terminal glycines required for N-terminal sortase-mediated labeling ...
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bioRxiv - Biochemistry 2021Quote: ... His6-mTRPM7-KD was subjected to TEV protease (New England Biolabs) digestion according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the GST-6xHis-tag was removed by TEV protease (#P8112S, NEB) and dArc1 and dArc2 proteins were further purified by size exclusion chromatography using Superdex S200 16/600 (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2024Quote: ... Protease Inhibitors) with recombinant PKA kinase (2500 U/mg, NEB-P600S) and 1 mM ATP overnight at 30°C and 250 rpm ...
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bioRxiv - Biochemistry 2024Quote: Purified MBP-fusion proteins were treated with TEV-protease (NEB, P8112S) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... nuclei of 106 cells plated on a 35 mm dish were isolated by addition of nuclei extraction buffer (NEB, 20 mM Hepes (KOH) pH 7.9 ...
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bioRxiv - Molecular Biology 2020Quote: ... TFIIIC was recovered after overnight incubation with TEV protease (100 units, NEB). TFIIIC was empirically determined to be saturating and TFIIIB and Pol III activities were determined to be limiting by multiple-round transcription assays.
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bioRxiv - Plant Biology 2022Quote: ... 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 x Protease Inhibitor Cocktail (NEB) as described before (Sun et al. ...
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bioRxiv - Bioengineering 2021Quote: ... the eluted MBP-RFP-sspB proteins were cut with TEV protease (NEB) using a digestion site between the MBP and the RPF sections ...
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bioRxiv - Microbiology 2021Quote: ... Then the His-tag was cleaved by incubation with TEV protease (NEB) for 18 h at room temperature ...