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Citations for New England Biolabs :
1 - 50 of 299 citations for Inactive Serine Protease 35 PRSS35 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Serine-35 mutations were constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Primers are listed in the supplemental methods.
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bioRxiv - Microbiology 2023Quote: ... and the prototype serine endopeptidase trypsin (New England BioLabs), placed within a fluorescence plate reader (Spectra max Gemini XPS ...
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bioRxiv - Microbiology 2020Quote: ... Catalytically inactive Mt2 (Mt2 C78A) variant was generated via site-directed mutagenesis (NEB, Q5 Site-Directed Mutagenesis Kit) using primers listed in the primer table (Supplementary Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... Catalytically inactive Mt2 (Mt2 C78A) variant was generated via site-directed mutagenesis (NEB, Q5 Site-Directed Mutagenesis Kit) using primers listed in the primer table (Table S1).
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bioRxiv - Cancer Biology 2021Quote: ... CDA E67Q catalytically inactive mutant42 was generated by site directed mutagenesis according to the manufacturer instructions (New England Biolabs). Lentiviral expression constructs were obtained by cloning CDA and CDAE67Q ORF into pCMV blasticidin DEST 706-1 vectors (Addgene ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant plasmids encoding a catalytically inactive 3Dpol (3Dneg) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). Nucleotides at position 6891 and 6892 of the CVB3/28 genome were mutated from AT to GC ...
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bioRxiv - Cell Biology 2020Quote: ... The kinase-inactive mutant was generated by a two-step PCR method using the Phusion High-fidelity kit (New England Biolabs). In the first step ...
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bioRxiv - Molecular Biology 2023Quote: ... and 35 µl rSAP (NEB) were added and sample was incubated for at least 8 h at 37 °C ...
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Mechanism of the ANT-mediated transport of fatty acid anions across the inner mitochondrial membranebioRxiv - Biophysics 2022Quote: ... and aspartic acid 134 to serine using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Austria). The sequences were verified by both DNA and amino acid sequencing ...
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bioRxiv - Genomics 2024Quote: ... was first generated by removing BsmBI restriction sites on pU6-sgRNA-EF1Alpha-puro-T2A-BFP (Addgene #60955)35 through Golden Gate Assembly (NEB E1602S). Then ...
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bioRxiv - Microbiology 2022Quote: ... EE62/63AA and the 2_87 double serine phospho-mimetic SE53/57EE or “SS-EE”) using Phusion-II polymerase (New England Biolabs) and standard protocols ...
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bioRxiv - Biochemistry 2023Quote: ... serine or aspartic acid were generated by engineering the corresponding constructs using Q5 site-directed mutagenesis (New England Biolabs, E0554). For this purpose ...
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bioRxiv - Cancer Biology 2021Quote: ... 35 µL of 10X NEB2.1 buffer (New England Biolabs) was added to each sample ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... WT hNEMF was generated by mutating the serine at site 86 to an arginine using Q5 site-directed mutagenesis (NEB, E0554S). pBI-dsRED empty vector was cloned using annealing primers flanked by NheI and EcoRV ...
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bioRxiv - Cell Biology 2023Quote: ... The C-KSR-GS construct replacing the vector-based linker (LELKLRILQS) by a glycine-serine rich linker (GGSSGGGGA) was generated by site directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, USA) of the C-KSR-EGFP plasmid ...
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bioRxiv - Genomics 2023Quote: ... then dissolved in a 35 μL elution buffer (EB, NEB) heated to 50°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Reactions were cycled 35 times with annealing temperatures calculated by NEB Tm calculator prior to loading on 2% agarose gels ...
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bioRxiv - Molecular Biology 2021Quote: ... 35 units of mung bean nuclease (New England Bio Labs (NEB)) and 0.02 mg/ml RNase A (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... using anchored oligo(dT)35 and random nonamers (New England Biolabs, USA), as per the protocol recommended by the manufacturer.
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bioRxiv - Molecular Biology 2022Quote: ... Protease K (P8107S, NEB). Antibodies used were ...
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bioRxiv - Biochemistry 2023Quote: ... 500 µL supernatant was applied to 35 µL amylose resin (New England Biolabs) or glutathione resin (Cytiva ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was used for PCR amplified for 35 cycles using Taq polymerase (NEB M0273) and respective primers (Table 1 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Samples 35 and 36 were prepared using the NEBNExt FFPE DNA Repair MIX (NEB). DNA fragment shorter than 20Kb were removed for sample 35 and 36 ...
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bioRxiv - Cell Biology 2023Quote: ... transferred to a 1.5ml tube and incubated with 35 μM benzylguanine conjugated fluorophore (NEB, S9136S) for 2 hours before being returned to the column ...
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bioRxiv - Biophysics 2020Quote: ... Factor Xa protease (New England Biolabs) was added to the MT solution at a molar ratio of 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x protease inhibitor cocktail (NEB), 2 mM N-ethylmaleimide (NEM) ...
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bioRxiv - Biochemistry 2021Quote: ... or V8 protease (New England Biolabs) in 25 mM NH4HCO3 overnight at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 20 µg factor Xa protease (NEB) and 5 mM CaCl2 with inversion at 10 °C overnight ...
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bioRxiv - Biophysics 2021Quote: Time-resolved experiments with TEV protease were performed by adding 1 μL TEV protease (New England Biolabs) after the 3rd time point to an imaging well containing 100 μL protein sample ...
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bioRxiv - Biophysics 2023Quote: ... concentrated protein was supplemented with 100 U TEV-His7 protease in 1X TEV protease reaction buffer (NEB) and incubated at 30 °C with agitation for 3 hr to cleave the His6-tag ...
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bioRxiv - Cancer Biology 2023Quote: ... Standard cloning was performed using PCR (35 μL ddH2O, 10 μL 5x HF Phusion buffer (NEB), 1 μL of 10 mM mixed dNTPs ...
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bioRxiv - Neuroscience 2021Quote: ... nuclei were resuspended in 200uL digestion buffer with 4 uL protease (an equal volume of protease K (NEB) was substituted if the manufacturer-provided protease was exhausted ...
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bioRxiv - Biochemistry 2022Quote: ... 3µL of TEV protease (New England BioLabs) was added per 200µL of CrossMAb-ACE2 fusions at ∼2µM in 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... 2µL of TEV protease (New England BioLabs) was added per 200 µL of scFv-ACE2 fusions at ∼4µM in 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... 1X protease inhibitor cocktail (New England Biolabs)] ...
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bioRxiv - Biophysics 2021Quote: ... and was TEV-protease (50 units; NEB) cleaved overnight at 4°C to remove the APEX tag ...
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bioRxiv - Plant Biology 2021Quote: ... or TEV protease (P8112S, New England BioLabs) were used for the cleavage of constructs in the backbone of pMAL-c5x and for His-MBP-[TEV]-ADCK3 ...
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bioRxiv - Genomics 2019Quote: ... The embryos were treated with protease (1 µL of 25 µg/µL Qiagen Protease, 1 µL of 10x NEB Buffer 4 ...
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bioRxiv - Cell Biology 2019Quote: ... at 30°C for 1 hour in a total volume of 50μl PMP phosphatase buffer (50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM DTT, 0.01% Brij 35, 1 mM MnCl2; New England Biolabs).
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bioRxiv - Cell Biology 2022Quote: Size markers 19 and 35 nucleotides long were dephosphorylated using 0.5 U/ μl CIP alkaline phosphatase (NEB M0290L) by incubating at 37°C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... beads were equilibrated in 1x PMP buffer (50 mM HEPES, 100 mM NaCl, 2 mM DTT, 0.01% Brij 35, pH 7.5; New England Biolabs). Kinase reactions were performed with 1 μL commercial CK1δ (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 35 ng/μL template DNA was incubated with 1.5 U/μL murine RNase inhibitor (New England Biolabs, USA), 0.2 μM inorganic pyrophosphatase ...
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bioRxiv - Molecular Biology 2024Quote: ... and first digested for 2h at 37°C with 35 units of AsiSI restriction enzyme (New England Biolabs). Then ...
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bioRxiv - Biochemistry 2020Quote: ... the resin was incubated with TEV Protease (NEB) for 3 h at room temperature ...
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bioRxiv - Genetics 2019Quote: ... and cleavage by TEV protease (New England Biolabs). The eluted chromatin was then used for a second purification step using Ni-NTA Agarose with nickel-charged affinity resin (Thermo Fisher) ...
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bioRxiv - Genetics 2020Quote: ... The indicated amount of furin protease (NEB P8077) was added per reaction tube in a final volume of 20 μL ...
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bioRxiv - Genomics 2020Quote: ... 0.5% NP40 and 1x protease inhibitors (P8107S, NEB) on ice for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated by centrifugation at 90,000 g for 35 min and the solubilized complex was incubated with amylose resin (NEB) for 2.5 h at 4 C ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated by centrifugation at 90,000 g for 35 min and the solubilized complex was incubated with amylose resin (NEB) for 2.5 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Insoluble material was pelleted at 119,000g for 35 minutes and the supernatant was bound to amylose resin (New England Biolabs). The resin was washed with lysis buffer and eluted with lysis buffer plus 10 mM maltose ...