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Citations for New England Biolabs :
551 - 600 of 9031 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Purified human gDNA was digested with the restriction enzyme HaeIII (NEB, Ipswich, Massachusetts) per the manufacturer’s instructions to yield an average of 347-bp DNA fragments22 ...
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bioRxiv - Genomics 2019Quote: ... mouse NIH/3T3 and human Jurkat DNA were from NEB (Ipswich, MA, USA). E14 genomic DNA was extracted with a DNeasy Blood and Tissue Kit (QIAGEN ...
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bioRxiv - Biophysics 2022Quote: Recombinant wild-type human histone H1.0 was used (H1; New England Biolabs M2501S). ProTαC and unlabeled ProTα were prepared as previously described (26) ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of in vitro transcribed 300 nM sgRNA or trL (produced as described in in vitro transcription protocol above) and 1 μL of 1 μM Cas9 nuclease (NEB, M0386S) were mixed and incubated for 10 minutes at 25°C to allow for RNP formation ...
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bioRxiv - Evolutionary Biology 2021Quote: Reverse transcription was performed by adding the following to the above reaction: 8 uL of 5x first strand buffer (NEB E7330L), 2 uL of 10mM dNTPs (each) ...
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bioRxiv - Developmental Biology 2021Quote: ... A T7 RNA polymerase binding site with short 5’-tail (aaaaTAATACGACTCACTATAG) was added to reverse primers for transcription using T7 RNA polymerase incorporating DIG labelled ribonucleotides (NEB, Roche). PCR amplified probe templates were confirmed by sanger sequencing.
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bioRxiv - Biochemistry 2020Quote: ... and 8 pmol of purified DNA was then used for in vitro transcription with T7 RNA polymerase (New England Biolabs Inc.). The resulting RNA was purified with Agencourt RNAClean XP beads supplemented with an additional 12% of PEG-8000 (3 volumes of 40% PEG-8000 was added to 7 volumes Agencourt RNAClean XP beads ...
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bioRxiv - Microbiology 2020Quote: ... and in vitro bacterial cultures was used as template in reverse transcription reactions using the Murine Moloney Leukemia Virus Reverse Transcriptase (NEB M0253S) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... the previous PCR products was used as template to amplify and convert into RNA via an in vitro transcription of high yield (New England Biolabs, E2040S); the RNA product above were converted back into single-stranded DNA via reverse transcription ...
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bioRxiv - Cancer Biology 2020Quote: ... 25uM RibOxi RT primer was then annealed to the reaction before ligation of 5’ RNA linker using T4 RNA ligase I at room temperature for 1 hour and subsequent reverse-transcription using Protoscript II (NE Biolabs, M0368S). cDNA was then purified with AmpureXP beads with 1:1.8 ratio ...
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bioRxiv - Genetics 2020Quote: ... In vitro transcription template was amplified with T7 promoter sequence containing primer and in vitro transcribed using T7 RNA polymerase (NEB, E2040S). The IVT RNAs were capped using Vaccinia virus capping enzyme (Cellscript ...
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bioRxiv - Cell Biology 2021Quote: sgRNAs of the target gene were obtained by in vitro transcription as described in (Leonetti et al., 2016) with modifications only to the PCR polymerase used (Phusion; New England Biolabs, M0530S). A 100 μL reaction of mNG21_10 HL-60 cells were electroporated (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... were heated to 65°C and slow-cooled to 37°C before reverse transcription with 5 U AMV-RT (NEB, M0277L) at 42°C for 1 hour ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription was carried out via SuperScript IV and cDNA was posteriorly amplified using Q5® High-Fidelity DNA Polymerase (NEB) with the ARTIC nCov-2019 primers and following the recommendations in the sequencing protocol of the ARTIC Network (https://artic.network/ncov-2019) ...
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bioRxiv - Microbiology 2022Quote: ... For this Reverse transcription was carried out via SuperScript IV and cDNA was posteriorly amplified using Q5® High-Fidelity DNA Polymerase (NEB) with the ARTIC nCov-2019 primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Toe-printing reactions were carried out in 5-µl aliquots containing a PURExpress transcription-translation coupled system (New England Biolabs, USA) to which the test template was added (16) ...
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bioRxiv - Microbiology 2023Quote: ... The AvcI DNA template for in vitro transcription was PCR amplified from pAvcI using Q5 High-Fidelity DNA Polymerase (NEB™). To incorporate the T7 promoter into the final AvcI DNA template ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting PCR product was purified by phenol-chloroform extraction and used for in vitro transcription using SP6 RNA-polymerase (NEB, M0207). The resulting sgRNA was purified using the High Pure PCR Cleanup Microkit (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... When needed the mRNA was polyadenylated after transcription using a recombinant poly-A polymerase as recommended by the manufacturer (NEB Biolabs).
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bioRxiv - Developmental Biology 2023Quote: ... a plasmid containing a full infectious clone of RV-M33 was linearized then added to an in vitro transcription reaction with Sp6 (New England Biolabs, M0207L). The resulting RNA was purified then polyadenylated (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... stop codon and 3’ T7 terminator were used as templates for the coupled in vitro transcription/translation PURExpress system (New England Biolabs, USA). The various SQS constructs used for cysteine crosslinking comprised an N-terminal 3xFLAG tag ...
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bioRxiv - Biochemistry 2023Quote: ... Ligated fragments were separated from free linkers on a 15% polyacrylamide TBE-Urea gel and then pooled and purified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB; M0368S). The ∼105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using CircLigaseII ssDNA Ligase (Biosearch Technologies ...
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bioRxiv - Immunology 2024Quote: ... A total of 1µg of the purified PCR product was used for in vitro transcription using the HiScribe T7 High Yield RNA synthesis (NEB E2040S), as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2024Quote: The Rapid Amplification of 5’cDNA end was carried out with Template Switching Reverse Transcription protocol (Wulf et al, 2019) provided by New England Biolabs (NEB #M0466). In the first step ...
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bioRxiv - Neuroscience 2024Quote: ... the resulting product was purified via column purification and underwent further amplification and conversion to RNA by a high-yield in-vitro transcription reaction using T7 polymerase (NEB, E2040S). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... in vitro transcription templates were prepared via 8 cycles of PCR using 2x Q5 Master Mix (New England Biolabs, Cat. M0492S) with a T7 promoter containing forward primer as previously described ...
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bioRxiv - Genomics 2020Quote: ... the circularized DNA was initially treated with human alkyl adenine DNA glycosylase (hAAG, NEB) to cleave the N-glycosidic bond of etheno-dA bases ...
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bioRxiv - Cell Biology 2022Quote: ... The 1970 bp DNA was then gel purified and 500 ng of DNA template was loaded into a 20-μL in vitro transcription reaction with 5,000 units of T7 RNA polymerase (New England BioLabs, product number M0251LVIAL), 1X RNAPol Reaction Buffer (New England BioLabs ...
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bioRxiv - Immunology 2019Quote: ... Then sgRNA template for in vitro transcription (IVT) was prepared by PCR amplification of Phusion high fidelity DNA polymerase (NEB Biolabs, Ipwich, MA), the PCR mixture was cleaned up by PCR cleanup reaction (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription and qPCR were performed in a one-step reaction using Luna® Universal One-Step RT-qPCR (NEB, Ipswich MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Template for in vitro transcriptions was amplified from PM-infected tissue cDNA using Phusion polymerase (M0530S/L, New England Biolabs, Ipswich, Massachusetts) and the T7 promoter (5’-TAATACGACTCACTATAGGGGG-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was performed with 2 μl of the reverse transcription positive reactions and Phusion High Fidelity DNA polymerase (NEB, Ipswich, MA) following manufacturer’s protocols on an Applied Biosystems 2720 Thermal Cycler ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was prepared from an equal amount of RNA by reverse transcription using NEB mMuLV reverse transcriptase (New England Biolabs, MA, USA). The list of primers used for amplification is shown in supplementary table 2 (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... 30 μL of the PCR product was added directly to a 50 μL in vitro transcription reaction with T7 RNA Polymerase (New England Biolabs, Beverly, MA) according to the manufacturer’s instructions and incubated for 3h at 37 ℃ ...
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bioRxiv - Biochemistry 2024Quote: ... 100 ng of template DNA was used in a 2 hour in vitro transcription reaction using the HiScribe T7 High Yield RNA (New England Biolabs, Cat. E2040S) kit according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Two-hundred and fifty ng of RNA were used for reverse transcription reactions using the LunaScript RT SuperMix (New England BioLabs cat# E3010). For the qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... Fusions to the human O6-Alkyl-DNA transferase (SNAP-tag, New England Biolabs, Beverly, MA) were expressed from plasmid pAGT-Xpress ...
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bioRxiv - Genetics 2020Quote: ... ~15nM (3ug) human genomic DNA or 30ng of gBlock in 1x Cutsmart buffer (NEB B7204) was incubated at 37°C for 15 minutes.
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bioRxiv - Cell Biology 2023Quote: Human ARHGAP18 constructs were created using Polymerase Chain Reaction (PCR) using New England Biolabs (NEB) Phusion High-Fidelity PCR Kit (catalog no ...
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bioRxiv - Immunology 2019Quote: ... Then sgRNA template for in vitro transcription (IVT) was prepared by PCR amplification of Phusion high fidelity DNA polymerase (NEB Biolabs, Ipwich, MA), the PCR mixture was cleaned up by PCR cleanup reaction (Qiagen ...
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bioRxiv - Physiology 2019Quote: ... The qPCR analyses were conducted with 5 μL of the reverse transcription reaction mixture with gene-specific primers (Supplementary Table S1) and the Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) was used ...
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bioRxiv - Microbiology 2021Quote: ... The transcription levels of the HO-1 gene were detected using the Luna Universal qPCR Master Mix (New England Biolabs Japan, Tokyo, Japan). Real-time PCR was performed using Mastercycler ep realplex2 (Eppendorf ...
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bioRxiv - Immunology 2023Quote: ... 1μg total RNA was used for reverse transcription with the NEB Template Switching Reverse Transcriptase Enzyme Mix (New England Biolabs, Ipswich, MA, USA) according to the recommendations of the manufacturer ...
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bioRxiv - Zoology 2023Quote: Reverse transcription was conducted with 10 µL of eluted nucleic acids using the ProtoScript® II Reverse Transcriptase (New England Biolabs, Massachusetts, USA). For each sample ...
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bioRxiv - Neuroscience 2020Quote: ... The purified RNA was then reverse-transcribed into cDNA using the Reverse Transcription System (Cat# E6300S, New England BioLabs® Inc., Ipswich, MA, USA). Quantitative RT-PCR analysis of the mRNA level of TNFα (TNFα ...
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bioRxiv - Neuroscience 2021Quote: ... Human α-synuclein cDNA from a pcDNA3.1 plasmid was restriction digested with EcoRI and HindIII (NEB), purified and ligated in the same sites of the pAAV2.5-THP backbone to generate the pAAV2.5-TH-α-syn plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... Snap tagged human D1DR constructs were labelled with Snap-cell 647 SiR (New England Biolabs, S9102S) as described previously (94).
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bioRxiv - Biophysics 2022Quote: ... The human μOR and V2R were amplified with a SNAP tag at their N-terminal (NEB) and subcloned in the pcDNA4/TO plasmid (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ...
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bioRxiv - Biochemistry 2022Quote: ... with recombinant proteins (200-500 ng) and yeast purified CK2 or recombinant human CK2 (NEB, P6010S). Proteins were resolved by SDS-PAGE and the gels were exposed to autoradiography or stained with Coomassie blue.