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Citations for New England Biolabs :
401 - 450 of 9563 citations for Human Phosphatidic Acid Phosphatase Type 2A PPAP2A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... dephosphorylated with Quick CIP alkaline phosphatase (New England Biolabs, Ipswitch, MA), gel-purified with the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 μl of Lambda protein phosphatase (P0753L, New England BioLabs) were incubated with the proteins at 30°C for 30 minutes.
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bioRxiv - Neuroscience 2023Quote: Dephosphorylation was performed on the resin with shrimp alkaline phosphatase (NEB) supplemented with 100 mM CaCl2 ...
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bioRxiv - Plant Biology 2024Quote: ... F4 was first dephosphorylated by incubating with calf intestinal phosphatase (NEB) for 2 h at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... alongside 5 U of quick alkaline phosphatase (calf intestinal, NEB #M0525S), all in a 30 µl rCutSmart buffer (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... Dephosphorylation reactions were performed with Lambda Protein Phosphatase (Lambda PP) (NEB). 1,000 units of Lambda PP were utilized per µmol of Ycf1 protein in a 500 µL reaction ...
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bioRxiv - Cell Biology 2019Quote: ... The CLN6ΔL2 construct was obtained by removing the codons for amino acids 155-222 using the Q5 Site-Directed Mutagenesis kit (New England Biolabs).
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We mutated aspartic acid 3420 to alanine (D3420A) and glutamic acid 3320 to alanine (E3320A) using a Q5 site-directed mutagenesis kit (E0552S, New England Biolabs, Ipswich ...
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bioRxiv - Biochemistry 2022Quote: ... the serine residues S240 and S250 in Dsn1 were mutated to aspartic acid using the Q5 site-directed mutagenesis kit (New England Biolabs) as described previously 19,20 ...
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bioRxiv - Molecular Biology 2022Quote: ... Amino acid substitutions and domain deletions were made using either Gibson assembly or the Q5 Site-Directed Mutagenesis Kit (NEB). Isogenic single and double mutant strains were generated via haploid mating ...
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bioRxiv - Genomics 2021Quote: ... Site-directed mutagenesis for amino acid substitution was performed using the Q5® Site-directed mutagenesis kit (New England Biolabs) according to the manufacture’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... A stop codon was inserted after amino acid residue 370 by site directed mutagenesis using Q5 Site-Directed Mutagenesis Kit (NEB), in order to express the truncated N1-370 construct ...
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bioRxiv - Microbiology 2024Quote: Amino acids substitutions in ompT were generated using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... site-directed mutagenesis of the codon encoding lysine at amino acid position 41 was altered to alanine using the Q5 site-directed mutagenesis kit (New England Biolabs) using pPM11 as a template ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The ZEB2 ORF was amplified with primers ZEB2_IndOE_F & _R and tagged with GFP-Flag by Gibson assembly into the pT2-CAG-MCS-2A-Puro plasmid linearized by restriction digestion with EcoRI (NEB, R3101S) and AgeI (NEB ...
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bioRxiv - Microbiology 2023Quote: ... TTP and IFN-λ1 were cloned using primers found in supplementary table 1 and inserted between pCW57-MCS1-2A-MCS2 EcoRI (NEB # R3101S) and BamHI (NEB # R3136S ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Briefly 2 µM of dsDNA guide was ligated into 10 ng of pSp-Cas9-(BB)-2A-GFP using 400 units T4 DNA ligase (NEB M0202) and incubated for 16 hrs at 16°C ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Oligonucleotides were ligated into pSp-Cas9-(BB)-2A-GFP plasmid that had been linearised by digestion with BbsI-HF (NEB R3539) and gel purified (Bioline BIO-52060 ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was depleted of ribosomal RNAs using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... Removal of rRNA was carried out by use of the NEBNext rRNA Depletion Kit Human/Mouse/Rat (NEB) followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina ...
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bioRxiv - Synthetic Biology 2022Quote: ... and sRNA plasmids using BsaI Type IIS Enzyme (New England Biolabs) and transformed into E ...
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bioRxiv - Bioengineering 2023Quote: ... Remnant wild-type DNA was degraded via DPN1 (New England Biolabs) digestion for two hours at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... the corresponding single and multiple amino acid substitutions were introduced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Molecular Biology 2021Quote: ... or more amino acid substitutions in SecPH with Q5 PCR methodology (Q5®Site-Directed Mutagenesis Kit, New England Biolabs, E0554) or PCR site-directed mutagenesis strategy ...
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bioRxiv - Immunology 2021Quote: ... were generated by introducing the corresponding amino acid mutations (Extended Data Fig. 5) using the Q5® Site-Directed Mutagenesis Kit (NEB) and per manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... The OprCAA mutant was produced by changing the key amino acids Cys143 and Met147 to alanine residues using the KLD Quickchange site-directed mutagenesis kit (New England Biolabs, UK) and specific primers containing both mutation sites (forward ...
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bioRxiv - Synthetic Biology 2021Quote: ... for 4 hours at 37°C and treated with Antarctic phosphatase (NEB) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... and 800 units of lambda protein phosphatase (λ-PPase; New England Biolabs) and incubated for 20 min at 30°C under slight agitation ...
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bioRxiv - Cell Biology 2020Quote: ... The cut backbone vector was treated with Antarctic Phosphatase (cat# M0289S, NEB) before ligation ...
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bioRxiv - Cell Biology 2020Quote: ... The cut backbone vector was treated with Antarctic Phosphatase (cat# M0289S, NEB) before ligation ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mU of phosphodiesterase II from Sigma (# P9041-10 UN) and 2 U of alkaline phosphatase from Biolabs (# M0290) were added and the mixture was incubated at 37°C ...
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bioRxiv - Systems Biology 2019Quote: ... The resulting RNA was de-phosphorylated with Antarctic Phosphatase (New England Biolabs) at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... was de-phosphorylated with 2 μL of Calf Intestinal Phosphatase (NEB M0290S) for 1 hour with shaking (1250 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were dephosphorylated using 1μL Lambda phosphatase at 30 °C (NEB; P0753S) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... RNAs were dephosphorylated with Alkaline Phosphatase (20 U; M0290S, NEB, Ipswich, MA) and T4 PNK (10 U ...
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bioRxiv - Microbiology 2021Quote: ... and subsequently 5’-dephosphorylated with Calf intestinal alkaline phosphatase (New England Biolabs). CviAII cuts on a sequence motif ‘CATG’ which is highly frequent on bacterial genomes ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with 400 units of λ phosphatase (NEB, Cat. No. PO753L) for 30 minutes at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... and 1.3 units of quick calf intestinal alkaline phosphatase (New England Biolabs) in buffer containing Tris-HCl (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... followed by the addition of calf intestinal alkaline phosphatase (CIP, NEB, 2Units). The mixture was incubated at 37°C for 1 hour to convert nucleotide 5’-monophosphates to their respective nucleosides ...
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bioRxiv - Microbiology 2019Quote: ... The digested pcDNA3.1-NefSF2V5-VC was treated with shrimp alkaline phosphatase (NEB), then ligated with the PCR products overnight at 16°C and transformed into TOP10 competent cells (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... NH4HCO3 (100 mM) and 5 units of alkaline phosphatase (CIP) (NEB, #M0525S) were added and the sample incubated for 2 hours (or 20 min ...
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bioRxiv - Immunology 2021Quote: ... The lysate and the precipitates were treated with Lambda Protein Phosphatase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... digested with AscI and then dephosphorylated using Antarctic Phosphatase (New England Biolabs). The 4mt-GCamP6f PCR product was phosphorylated using T4 Polynucleotide Kinase (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... incubated with λ protein phosphatase (40 units/μL) (P0753S, New England Biolabs) or recombinant TOPP4-GST/HIS for 1 h at 30□ ...
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bioRxiv - Immunology 2022Quote: ... and subsequently incubated with 4 U of alkaline phosphatase (New England Biolabs) at 37°C for 30 min to hydrolyze unreacted NTPs ...
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bioRxiv - Microbiology 2022Quote: ... The linear backbone was then dephosphorylated using shrimp alkaline phosphatase (rSAP, NEB) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Lysed protoplasts were treated with calf intestinal phosphatase (CIP) (New England Biolabs) for 60 minutes at 37°C (1 unit per μg of total protein) ...
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bioRxiv - Cell Biology 2023Quote: ... mCherryC1 empty vector was also digested along with shrimp alkaline phosphatase (NEB) treatment ...