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Citations for New England Biolabs :
651 - 700 of 9563 citations for Human Phosphatidic Acid Phosphatase Type 2A PPAP2A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed, treated with T4 Polynucleotide Kinase (PNK, with no 3’-phosphatase activity) (NEB, M0236S), and miRNA-RNA chimeras were produced with T4 RNA Ligase I (NEB ...
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bioRxiv - Immunology 2020Quote: ... Plasmid digestions were performed following manufacturer recommendations and generally subjected to Antarctic phosphatase (New England Biolabs # M0289S) treatment to prevent potential re-annealing ...
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bioRxiv - Biochemistry 2020Quote: ... treated with lambda phosphatase protein after purification at 30°C for 1 hr (New England BioLabs, P0753L), or co-expressed in BL21 cells with RFP-lambda phosphatase ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: GBL phosphate (8) was incubated with 5 units of calf intestinal alkaline phosphatase (CIP, New England Biolabs) in 50 mM HEPES buffer (pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: 20 µg protein lysate was incubated with lambda phosphatase according to the manufacturer’s protocol (New England Biolabs). Afterwards ...
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bioRxiv - Cell Biology 2022Quote: Recombinant condensin II holo(WT) at 500 nM was mixed with or without λ protein phosphatase (NEB) at a final concentration of 400 U/µL in 1x NEBuffer for Protein MetalloPhosphatases supplemented with 1 mM MnCl2 and incubated at 30°C for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... For CAK binding assays CDK2 was dephosphorylated by washing beads once in 1x λ-phosphatase buffer (NEB) supplied with 1 mM MnCl2 (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... The antisense strand with a 3′ phosphate was radiolabeled by T4 Polynucleotide Kinase (3’ phosphatase minus) (NEB) and [γ-32P]ATP ...
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bioRxiv - Microbiology 2020Quote: ... was sequentially digested with BamH1 and Kpn1 followed by dephosphorylation with shrimp alkaline phosphatase (New England Biolabs). Ligation of the product and linearized vector was performed overnight at 14°C with T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications (Fig. 1A): The input RNAs were treated with Antarctic Phosphatase (NEB, Ipswich, MA) for 1 h at 37°C to remove any 5’end phosphates ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA fragments were directly 3’-end dephosphorylated using 5 U of Antarctic Phosphatase (New England Biolabs, UK) for 30 min at 37°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... which was previously digested with the same enzymes and de-phosphorylated with Antarctic phosphatase (New England Biolabs). The templates for the inserts of libraries C45-I and II ...
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bioRxiv - Molecular Biology 2021Quote: ... The pBluescript plasmid was cut by EcoRV and dephosphorylated by Calf Intestinal Alkaline Phosphatase (CIP) (NEB, M0290L), then ligated with the phosphorylated PCR fragments by T4 DNA Ligase (NEB ...
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bioRxiv - Immunology 2020Quote: ... The amplified DNA was then treated with recombinant Shrimp Alkaline Phosphatase and Exonuclease 1 (New England Biolabs), and sent to ELIM Biopharm for sequencing ...
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bioRxiv - Microbiology 2020Quote: ... beads were resuspended in 200 μl CIP mix (20 units of calf intestinal alkaline phosphatase (M0290, NEB) in CIP buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µl freshly made 1 M NH4HCO3 and calf intestinal alkaline phosphatase (1 U, New England Biolabs) were added ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA fragments were directly 3’-end dephosphorylated using 5 U of Antarctic Phosphatase (New England Biolabs, UK) for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... polynucleotide kinase (PNK) and Antarctic phosphatase used during cloning were all purchased from NEB (Ipswich, MA, USA). Purification of PCR products was carried out using the Qiagen PCR purification kit according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... before the unbound fraction containing cleaved Gβ1γ2 was dephosphorylated by treatment with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Biochemistry 2023Quote: ... 50 ul of aqueous solution were recovered and treated with 0.3 U of calf intestinal phosphatase (NEB) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... This plasmid was then linearized with the AvrII enzyme and dephosphorylated with Antartic Phosphatase (New England Biolabs) in order to insert an extra double TY tag ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 µg of purified recombinant WRN protein was treated with Lambda Protein Phosphatase (LPP, New England Biolabs) in 1X PMP buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... and the vector was treated with Mung Bean Nuclease and Alkaline Phosphatase Calf Intestinal (New England Biolabs). The blasticidin resistance gene was isolated from a plasmid pKM808 using XhoI/SalI (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... the vector was dephosphorylated by the addition of 2 U of shrimp alkaline phosphatase (New England BioLabs) for the last 30 min of the restriction reaction ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2023Quote: ... TCA extract in Laemmli buffer was diluted 10-fold in 1X PMP buffer (NEB, Protein Metallo Phosphatases) supplemented with 1 mM Mncl2 ...
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bioRxiv - Biochemistry 2023Quote: ... guide RNAs were first dephosphorylated using shrimp alkaline phosphatase (rSAP, 0.05 U/µL; New England Biolabs, M0371) at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA or immunoprecipitated RNA (MIWI or MILI) was de-phosphorylated with Shrimp Alkaline Phosphatase (M0371, NEB) and end-labeled using T4 polynucleotide kinase (M0201 ...
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bioRxiv - Neuroscience 2024Quote: ... purified ∼25−50-nt RNA fragments underwent treatment with recombinant shrimp alkaline phosphatase (New England Biolabs; M0371) to remove 5’-and 3’-phosphates ...
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bioRxiv - Genomics 2021Quote: ... gDNA was digested with the CspCI Type IIB restriction enzyme (IIB-REase - New England BioLabs, Inc.) which has shown to yield a high marker density in triatomine65 ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by digestion of the wild-type strand with the enzyme BsurI (New England Biolabs, R0581S)[27] ...
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bioRxiv - Microbiology 2020Quote: ... The wild-type Chlamydia trachomatis ct276 gene was amplified by PCR with Phusion DNA polymerase (NEB) using 100 ng C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Golden gate (Type-IIS) cloning was conducted using chemically competent Escherichia coli DH5α cells (NEB, C2987H). Construction of destination vectors with the toxic selection marker ccdB was done with chemically competent One Shot ccdB Survival 2 T1R E ...
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bioRxiv - Immunology 2022Quote: ... with short homologies for Gibson assembly and cloned into human IgG1 or human IgL2 expression vectors using the NEB Hifi DNA Assembly mix (NEB, Cat#E2621L). Plasmid sequences were verified by Sanger sequencing (Genewiz).
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bioRxiv - Bioengineering 2020Quote: ... Single amino acid substitutions were introduced utilizing the KLD Enzyme Mix (NEB) following PCR amplification with mutagenic primers (Genewiz) ...
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bioRxiv - Molecular Biology 2023Quote: ... nucleic acids extracted from SPARDA were phosphorylated by T4 polynucleotyide kinase (NEB) according to the manufacturer’s protocol and were separated by 18% PAGE ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... Human plasma was treated with PNGase F (NEB; P0704S) or a mixture of O-Glycosidase (NEB ...
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bioRxiv - Biophysics 2020Quote: ... as with commercially available human H1 (hH1) (M2501S; NEB), sharing 96.5% identity with mH1 (Fig ...
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bioRxiv - Biochemistry 2023Quote: ... and human casein kinase II (New England BioLabs, #P6010).
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... Recombinant human Histone H2A (New England BioLabs, Catalog # M2502S) or H4 (New England BioLabs ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were cleaned using 1U of Exonuclease I and Antarctic Phosphatase (New England Biolabs, Ipswich, Mass., USA) and incubating at 37 °C for 20 min followed by 10 min enzyme denaturation at 80 °C ...
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bioRxiv - Bioengineering 2019Quote: pRM02-Spec was linearized by AgeI and simultaneously dephosphorylated by Shrimp Alkaline Phosphatase (New England Biolabs, Cat. # M0371S). The 4.6 kb PSF expression cassette was PCR amplified from pPSF using primer pair PSF-AgeI-For/ PSF-AgeI-Rev and double digested with DpnI/AgeI ...
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bioRxiv - Bioengineering 2019Quote: pRM01-Spec was linearized by AgeI and simultaneously dephosphorylated by Shrimp Alkaline Phosphatase (New England Biolabs, Cat. # M0371S). The RSLB1 expression cassette was PCR amplified from plasmid pRSLB1 using primer pair R32CerLBAgeIfor/newPCFAgeIrev (PCR product size ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μg of purified protein complexes were treated with 2,000 U of Lambda Protein Phosphatase (New England Biolabs) in 200 μl dephosphorylation buffer (50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2020Quote: ... but instead of ATP 1 mM MnCl2 was added and 1 μl (400 U) of λ phosphatase (NEB) was used instead of PKR.
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bioRxiv - Cell Biology 2021Quote: ... One of each pair was treated with 0.5 μl of calf intestinal phosphatase (CIP) (New England Biolabs #M0290) before the pair were incubated at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... The unbound fraction was then incubated for 1 hour at 4 °C with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... Approximately 3-5μg of native genomic DNA were dephosphorylated with 0.05U/μl of shrimp alkaline phosphatase (rSAP) (New England BioLabs) to reduce the amount of off-target molecules in the final SMRTbell® library ...
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bioRxiv - Neuroscience 2020Quote: ... We used direct ligation of an RNA adapter to the harvested RNA after initial dephosphorylation(NEB Antartic Phosphatase) of free ends ...
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bioRxiv - Molecular Biology 2020Quote: ... the amplified products were cleaned using Exonuclease-Shrimp Alkaline Phosphatase (Exo-SAP) mixture (New England Biolabs, Ipswich, Massachusetts) and sequenced bidirectionally using BigDye v3.1 terminator kit in ABI 3500XL Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... 50mM Tris (pH 8.5-8.8)) containing protease and phosphatase inhibitors (cOmplete Mini, PhosSTOP, Roche; 2mM Sodium Orthovanadate, NEB), sonicated to shear chromatin and centrifuged to remove cellular debris ...