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Citations for New England Biolabs :
1 - 50 of 10000+ citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... T4 PNK 3’ phosphatase minus (New England BioLabs, M0236S) was used.
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bioRxiv - Molecular Biology 2019Quote: ... 0.5 ul T4 PNK - 3’ phosphatase minus (NEB M0236L). Samples were then washed 3 times on a magnetic rack with PNK wash buffer ...
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bioRxiv - Microbiology 2020Quote: ... 3 µl Antarctic phosphatase [5 U] and 7.32 µl Antarctic phosphatase buffer (both New England BioLabs, Germany). The reaction was heat-inactivated at 85°C during 15 min ...
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bioRxiv - Genetics 2023Quote: ... 1 μl T4 PNK (3′ phosphatase minus, New England BioLabs)) ...
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bioRxiv - Physiology 2022Quote: RNA-sequencing libraries were prepared by depleting eukaryotic ribosomal RNA with the NEBNext rRNA Depletion Kit prior to library synthesis with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and addition of multiplex oligos using the Unique Dual Index Primer Pairs Set 3 (NEB). Library quality control was performed using Qubit and Bioanalyzer 2100 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the Unique Dual Indexing Kit (New England Biolabs), with reagent volumes reduced to one-fifteenth of recommended volumes ...
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bioRxiv - Synthetic Biology 2020Quote: ... This was done in 50 μl reactions using 3 μl dual index primers (NEB), 135 ng purified PCR product from the previous reaction and 25 μl Q5 high fidelity master mix (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... Mutations to the specificity of guides in pTREX-n-Cas9 were performed using a Q5 mutagenesis kit (NEB, Ipswich, Massachusetts). Guides targeting the ELO genes were inserted using the primers described in Table 1 ...
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bioRxiv - Plant Biology 2021Quote: ... The antisense strand with a 3′ phosphate was radiolabeled by T4 Polynucleotide Kinase (3’ phosphatase minus) (NEB) and [γ-32P]ATP ...
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bioRxiv - Neuroscience 2022Quote: ... and Unique Combinatorial Dual index kit (New England Biolabs #E6442S,) with modifications outlined below ...
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bioRxiv - Microbiology 2020Quote: ... Mutations to the specificity of guides in pTREX-n-Cas9-noTags was performed using a Q5 mutagenesis kit (NEB, Ipswich, Massachusetts). Guides targeting the mitochondrial glutamate dehydrogenase (mGDH ...
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bioRxiv - Developmental Biology 2021Quote: ... Grb10a was amplified from cDNA by high specificity PCR (NEB Q5 Hot Start) with primers flanking open reading frame (Table 1) ...
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bioRxiv - Microbiology 2019Quote: ... Insert and dual indexed libraries were prepared using a modified NEBNext Ultra DNA II dual indexing kit (New England Biolabs) without size selection ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and dual indexing from the NEBNext Multiplex Oligos kit (NEB #7600) following manufacturer protocol including beads size selection for 200 bp inserts ...
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bioRxiv - Molecular Biology 2022Quote: ... 1000 ng of DNA diluted in 27 μL of nuclease-free water was dephosphorylated by the addition of 3 μL of 10X CutSmart Buffer and 3 μL of Quick Calf Intestinal Phosphatase (NEB) and then incubated at 37ºC for 10 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Precipitated RNA 3’ends were first dephosphorylated by shrimp alkaline phosphatase (SAP) (NEB M0371), and 3’ RNA linker (20 µM ...
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bioRxiv - Molecular Biology 2022Quote: ... and/or 10 U of T4 PNK (wild-type or 3’ phosphatase minus, NEB). For RtcB ligation ...
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bioRxiv - Molecular Biology 2023Quote: ... Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit). Paired-end 2×25 bp sequencing was performed on a NextSeq500 Illumina Sequencer.
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bioRxiv - Cell Biology 2023Quote: ... Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit). Paired-end 2×25 bp sequencing was performed on a NextSeq500 Illumina Sequencer.
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bioRxiv - Genetics 2019Quote: ... phosphatase treated (Antarctic phosphatase, NEB M0289S), and ligated together using T4 ligase (NEB ...
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bioRxiv - Genomics 2022Quote: ... Libraries were dual-indexed (NEBNext Dual Index Primers Set 1, NEB E7600S), and sequenced on an Illumina NextSeq 2000 instrument with P3 300 cycle reagents ...
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bioRxiv - Developmental Biology 2021Quote: ... Top and bottom guide RNAs were annealed with T4 Polynucleotide Kinase (3’ phosphatase minus, NEB) and T4 DNA Ligase Buffer (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... and 3 µg/ml leupeptin were incubate with 17.5 U alkaline phosphatase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of RNAse inhibitor and 3 μl of Antarctic phosphatase (New England BioLabs Inc.). We then performed a kinase treatment adding 4 μl of T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Genomics 2022Quote: ... denatured and phosphorylated with 10 U of 3’-phosphatase-minus T4 polynucleotides kinase (New England Biolabs) at 37 °C for 30 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... low passage HEK293T cells were co-transfected a dual Cas9 and gRNA expression vector (pX330-T2) targeting the human AAVS1 locus71 and a PmeI-linearized (NEB) repair template comprising a constitutively expressed YFP-HygR-expression cassette with a BxB1 attP recognition site flanked by 800bp homology arms for the human AAVS1 locus (pROC079) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using dual indices (NEB #E7600). We quantified libraries by Bioanalyzer and with a Quant-iT kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Dual index primers (NEB #E7780S) for sequencing on Illumina MiSeq and NovaSeq platforms were added by amplifying the tagmented samples using Q5 polymerase (NEB #M0544S ...
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bioRxiv - Developmental Biology 2024Quote: ... NEBnext dual indices (NEB #E7600S) were added to the amplicons using NEBNext® Ultra™ II Q5® Master Mix (NEB ...
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bioRxiv - Immunology 2020Quote: ... λ-phosphatase (NEB), Phalloidin (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... were cloned into a dual U6 (1+3 promoter) expression construct pCFD4 provided by the Mann lab using Gibson assembly (New England Biolabs). The donor construct and the guide RNA construct were injected into wlig4 ...
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bioRxiv - Systems Biology 2023Quote: Mutated or wild type sequences of RORC 3’UTR were cloned into the dual GFP-mCherry reporter using MluI-HF and PacI restriction enzymes (NEB) as described above ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed, treated with T4 Polynucleotide Kinase (PNK, with no 3’-phosphatase activity) (NEB, M0236S), and miRNA-RNA chimeras were produced with T4 RNA Ligase I (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA fragments were directly 3’-end dephosphorylated using 5 U of Antarctic Phosphatase (New England Biolabs, UK) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µl freshly made 1 M NH4HCO3 and calf intestinal alkaline phosphatase (1 U, New England Biolabs) were added ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA fragments were directly 3’-end dephosphorylated using 5 U of Antarctic Phosphatase (New England Biolabs, UK) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: Specificity of anti-H3K9Me3 was tested against two types of substrates: recombinant histone H3 (New England BioLabs, M2507S), which lacked methylation ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Genomics 2024Quote: ... and individually dual-barcoded using a New England Biolabs Ultra II Directional kit (NEB #E7765). Individually barcoded samples were pooled and sequenced paired end and 150 bp in length on two lanes of a Novaseq at the University of New Hampshire Hubbard Center of Genome Studies.
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bioRxiv - Developmental Biology 2019Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II library prep kit with the NEB Unique Dual Index primer set kit (New England Biolabs). Samples were sequenced at the Northwestern University NuSeq Core Facility on an Illumina HiSeq 4000 on single-end 50 bp mode ...
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bioRxiv - Genetics 2021Quote: ... incorporating unique dual-indexing (NEB, E6440S). Final library sizes ranged from 303-bp to 327-bp (Agilent TapeStation 2200) ...
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bioRxiv - Cancer Biology 2023Quote: ... with Dual Index Primers (NEB #E7600) were used for library preparation with ChIP-seq.
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bioRxiv - Molecular Biology 2023Quote: ... NEBNext dual adaptors (New England Biolabs) were then individually applied to barcode each library prior to sequencing of 100 base pair paired-end reads ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were diluted to 1.0 mg/mL protein using homogenization buffer and incubated with 20 U/μL lambda phosphatase in MnCl2 and enzyme buffer as supplied with the lambda protein phosphatase kit (New England Biolabs, Evry-Courcouronnes, France) for 3 h at 30 °C ...
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bioRxiv - Biochemistry 2020Quote: ... lambda phosphatase (NEB) in final concentration 90 U/μl and 1 mM MnCl2 were added ...
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bioRxiv - Molecular Biology 2021Quote: Phosphatase (NEB P0753S)
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bioRxiv - Molecular Biology 2022Quote: ... Antarctic phosphatase (NEB) was added after 15 minutes to the RFP+TORNADO Broccoli digest only ...
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bioRxiv - Synthetic Biology 2020Quote: ... Antarctic Phosphatase (NEB), and T4 PNK (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... Antarctic phosphatase (NEB) was added after 15 minutes to the RFP+TORNADO Broccoli digest only ...
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bioRxiv - Cell Biology 2023Quote: In vitro dephosphorylation was performed using lambda phosphatase kit (New England Biolabs, P0753S). Following the immunoprecipitation step ...