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Citations for New England Biolabs :
251 - 300 of 10000+ citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... PCR mutagenesis to create site-directed mutants of malM 3’-UTR and cspE 3’-UTR was conducted with the Q5 site-directed mutagenesis kit (New England Biolabs) using end-to-end primers designed with NEBaseChanger ...
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bioRxiv - Genetics 2022Quote: The detection of aberrant Scyl1 transcripts was performed by RT-PCR using primers designed to amplify sequences between exon 2 to exon 5 (RT-Scyl1_F21 5’-CGCAGTGTCCATCTTCGTGTA-3’ and RT-Scyl1_R51 5’-CCCGGCAGTTCTGCAGGAA-3’) following the OneTaq One-Step RT-PCR Kit (New England Biolabs, E5315S) procedure ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was PCR amplified (primers hFUR Exon 1-3 PCR For and hFUR Exon 1-3 PCR Rev) and purified (Monarch PCR & DNA cleanup kit, NEB). The resulting PCR product was sequenced using the hFur Exon 1 Seq primer.
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bioRxiv - Microbiology 2023Quote: ... 10 μM SARS-CoV-2 E-gene Forward (5’- ACAGGTACCTTAATAGTTAATAGCGT-3’) and Reverse (5’-ATATTGCAGCAGTACGCACACA-3’) primers were used with Luna Universal One-step RT-qPCR kit (New England Biolabs) and added to 1 μl supernatant (5μl total ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA resistant mCherry-ALG-2 was created by mutagenesis (primers sequences 5’-ATTTCGATGTTTGACCGTGAGAAC-3’, 5’-AATGGACCTGACAGTCACTGGATTAAA-3’) using a Q5 Site-Directed Mutagenesis Kit (E0554S, NEB). The plasmid encoding IST1-GFP is as described (63 ...
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bioRxiv - Molecular Biology 2024Quote: ... The CHD4-GFP mutant was cloned with selective primers (fwd, 5’-GGATGCTACAGGTGGAACCCTGCACCCCTA-3’; rev, 5’-GCCCAGGCCCGACGCCAT-3’) and a Q5 site-directed mutagenesis kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... while the RBD constructs were introduced in frame to the mu phosphatase signal sequence and incorporated within pcDNA3.1 via Gibson assembly (NEBuilder HiFi DNA Assembly Cloning Kit, New England Biolabs).
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bioRxiv - Cell Biology 2020Quote: ... 40 µl of the cleared lysates were incubated with 1 µl Lambda Protein Phosphatase (λ phosphatase) (New England Biolabs), 5 µl 10x buffer for Protein MetalloPhosphatases (New England Biolabs) ...
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bioRxiv - Cell Biology 2021Quote: ... beads were pelleted and resuspended in 100 μl of phosphatase buffer with lambda protein phosphatase (P0753; New England Biolabs) before agitation at 30°C for 30 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: We prepared dual indexed libraries according to the instruction manual using the NEBNext Ultra II DNA Library Prep Kit for Illumina and the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1, New England Biolabs, Ipswich, Massachusetts, USA) and following the recommended conditions of bead-based size selection according to distribution of DNA fragments per sample ...
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bioRxiv - Genetics 2020Quote: ... following rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... Human FBP1 mutants were constructed by Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) in the PCDH-V5-FBP1 vectors ...
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bioRxiv - Biochemistry 2020Quote: ... the human Rab1b 3-174aa (referred to as Rab1b)-encoding DNA was cloned into a modified pMAL vector (New England Biolabs), resulting in a construct with a N-terminal hexahistidine (6xHis ...
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bioRxiv - Molecular Biology 2020Quote: ... A mix of 5 μl 3’Ligation Reaction Buffer 2x (NEB-kit) and 1.5 μl of 3’Ligation Enzyme Mix (NEB-kit ...
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bioRxiv - Genetics 2022Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligase 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligase 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligas’ 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligase 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Klenow-mediated addition of an adenine to the 3’ end of the DNA fragments was performed using the Klenow fragment (3’→5’ exo-) kit (NEB, M0212L) by combining the 32 μl sample with 5 μl 10X Klenow Buffer NEB 2 ...
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bioRxiv - Neuroscience 2020Quote: ... Equal volumes of each immunoprecipitated sample were then aliquoted into two tubes and beads were then resuspended with 100 microliters of lambda phosphatase assay buffer containing 400 units of lambda protein phosphatase (NEB), or with 100 microliters of lambda phosphatase assay buffer alone ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysate obtained from lysis with 1% NP-40 lysis buffer phosphatase inhibitor were mixed with lambda phosphatase (New England Biolabs) and incubated at 30°C for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... Peptide bound beads were washed with 100 µL 1x lambda phosphatase buffer prior to incubating with lambda protein phosphatase (P0753; NEB).
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bioRxiv - Biochemistry 2022Quote: ... purified KRAS protein was mixed with GMP-PNP (molar ratio of 10:1 GMP-PNP:KRAS) and calf intestinal alkaline phosphatase (NEB cat# M0290, 3 units per mg of KRAS). The reaction mixture was incubated for 3 hours at room temperature and then purified by size-exclusion chromatography on a 10/300 Superdex 75 GL column (GE Healthcare ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated with Antarctic Phosphatase (New England Biolabs) at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 U of alkaline phosphatase from Biolabs (# M0290) and 5 mU of phosphodiesterase I from Sigma (# P3243-1VL ...
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bioRxiv - Molecular Biology 2020Quote: ... and 400U (1μl) of λ-phosphatase (NEB P0753S) was added to the phosphatase sample ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by incubation with Antarctic Phosphatase (NEB, 5U) for an additional 2h at 37°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 1.5 unit of Shrimp Alkaline Phosphatase (NEB). The reaction was incubated at 37 °C for 30 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... and dephosphorylated using Antarctic phosphatase (New England Biolabs). After ligation of insert and vector DNA with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... 2 μl Antarctic Phosphatase [5k U/mL] (NEB) and 1 μl RNase inhibitor and incubating at 37 °C for 30 minutes with shaking ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we treated the plasmids with Antarctic phosphatase (NEB) to minimize their self-ligation ...
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bioRxiv - Microbiology 2021Quote: ... followed by deactivation with Antarctic Phosphatase (NEB M0289S).
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bioRxiv - Bioengineering 2021Quote: ... Antarctic phosphatase (5,000 units/mL) (New England Biolabs), Phosphodiesterase 1 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... and dephosphorylated with Antarctic phosphatase (New England Biolabs). The fragments were purified with E.Z.N.A ...
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bioRxiv - Neuroscience 2020Quote: ... 4 μl of Calf intestinal alkaline phosphatase (NEB) was added to dephosphorylate the RNA for 15 min at 37 °C ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μl shrimp alkaline phosphatase (New England Biolabs), 2 μl RNaseOUT (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μl λ-phosphatase (NEB, 400,000 U/μl), and 8 μl of phosphatase reaction buffer (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... and 8 μl of phosphatase reaction buffer (NEB). After incubation at 30°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... samples were treated with Lambda Protein Phosphatase (NEB) for 30 min at 30 °C followed with an additional four times washing step before they were blocked ...
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bioRxiv - Cell Biology 2021Quote: ... 0.4 µg/ml λ phosphatase (NewEngland Biolabs, P0753S) and 1x phosSTOP cocktail (Millipore-Sigma ...
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bioRxiv - Microbiology 2021Quote: ... and 400 units of lambda (λ) phosphatase (NEB) for 24 hours at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 2 μl of 10× Antarctic Phosphatase buffer (NEB), 1 U Antarctic Phosphatase ...
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bioRxiv - Systems Biology 2020Quote: ... de-phosphorylated with Antarctic phosphatase (New England Biolabs) and concentrated using PCR cleanup columns (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... treated with calf intestinal phosphatase (New England Biolabs), and gel-purified ...
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bioRxiv - Biochemistry 2020Quote: Calf Intestinal alkaline phosphatase (CIP, New England Biolabs) was conjugated on UltraLink Biosupport beads (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Alkaline phosphatase (cat # M0290S) was purchased from NEB. DTAF-streptavidin (cat # 016-010-084 ...
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bioRxiv - Cell Biology 2022Quote: ... restriction digestion and dephosphorylated with Antarctic Phosphatase (NEB) prior usage in transfection.
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bioRxiv - Synthetic Biology 2021Quote: ... and 20 units of calf alkaline phosphatase (NEB) were added to the cooled solution and incubated at 37°C for 1 hour ...
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bioRxiv - Biophysics 2021Quote: ... and 2.5 μL λ phosphatase (New England Biolabs) was added per 50 μL concentrated protein ...
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bioRxiv - Biochemistry 2021Quote: ... de-phosphorylated using Alkaline Phosphatase (New England Biolabs) and purified by phenol:chloroform:isoamyl alcohol (25:24:1 ...