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Citations for New England Biolabs :
51 - 100 of 2494 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... TA overhangs were added by incubating the insert for 10 min at 72 °C with 50mM DNTPs and Taq polymerase (NEB), in order to perform TA cloning into a PCR4 TOPO vector (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mM EDTA) at 65°C for 15 minutes and later the proteins were digested by 10U Proteinase K (NEB) at 37°C for 30 minutes ...
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bioRxiv - Genomics 2019Quote: ... The ligase was killed by heating at 65°C for 10 min and then 5 µl of 10x reaction buffer 3 (NEB), 34 µl of H2O and 1 µl of PstI (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... coli or Expi293™ cells underwent denaturation at 95 °C for 10 min in glycoprotein denaturing buffer (New England Biolabs) followed by immediate cooling on ice for 10 s ...
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bioRxiv - Cancer Biology 2021Quote: ... and after stringent washing in the same buffer at 55°C for 10 min ligated to the custom SCRiBL adapter using 1600 units of T4 DNA ligase (NEB) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... at 98,000 × g at 4 °C for 45 min and the supernatant loaded onto a self-packed column with ∼10 ml of amylose resin (NEB) pre-equilibrated in 50 mM Tris-HCl pH 9.0 ...
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bioRxiv - Genomics 2022Quote: ... Digestion was performed overnight by adding 50 μL of DpnII (Capture-C) or HindIII (5C) buffer and 10 μL of high-concentration DpnII or HindIII (NEB) and incubating samples at 37°C in a thermomixer ...
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bioRxiv - Cell Biology 2020Quote: ... Four hundred ng of total RNA extracted from pools of 250 mouse oocytes was ligated to 400 ng of P1 anchor primer (5’-P-GGT CAC CTT GAT CTG AAG C-NH2-3’) in a 10-µl reaction using T4 RNA ligase (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Genomics 2019Quote: ... were annealed to form adapters by incubating at 85°C for 10 min in 1x T4 DNA ligase buffer (NEB) before cooling gradually by transferring the heated block to the bench before dilution in 1x T4 DNA ligase buffer to working concentrations.
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bioRxiv - Genetics 2019Quote: ... DNA samples were incubated at 20°C for 4h without rotation in a mix of 10 µl of 10x NEB2 buffer (New England Biolabs), 1 mM of a dNTPs mix (10 µl) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR of 10 cycles with an annealing temperature 61°C was performed using Q5 High-Fidelity DNA Polymerase (#M0491S, New England Biolabs) and BEL and AL primers on 5μL of I-SceI digested DNA purified from the ChIP procedure ...
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bioRxiv - Genomics 2019Quote: ... samples were digested with 10 U pshAI in 1x CutSmart buffer at 37°C for 3 or more hours (NEB).
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bioRxiv - Genomics 2020Quote: ... and half of the DNA was 3’-end labeled for 1 h at 37 °C in a 10-μl reaction containing 6 units of terminal deoxynucleotidyl transferase (New England Biolabs), 0.25 mM CoCl2 ...
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bioRxiv - Cell Biology 2019Quote: ... 95°C for 10 minutes and equal amounts of lysates (180 ug) were treated with either 1uL of EndoH (NEB), PNGase F (NEB) ...
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bioRxiv - Genomics 2020Quote: ... Zyagen samples were amplified with PBC096 barcoding for 8-10 cycles with both LongAmp (female, 62°C annealing; NEB, US) and PrimeSTAR GXL (male and female ...
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bioRxiv - Genomics 2019Quote: ... One million nuclei aliquots were pelleted by 1,000 x g at 4°C for 10 min and resuspended in 200 µl of GpC methyltransferase M.CviPI (NEB M0227L) reaction containing 1X GC Reaction Buffer ...
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An egg-derived sulfated N-Acetyllactosamine glycan is an antigenic decoy of influenza virus vaccinesbioRxiv - Immunology 2021Quote: ... 25 ug of the 2020 QIV was denatured for 10 minutes at 75°C and treated with the Protein Deglycosylation Mix II (New England Biolabs) for 30 minutes at 25°C and 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... lysate volume equivalent to 20-40 µg total protein was first denatured at 100°C for 10 min in 1X glycoprotein denaturation buffer (NEB). Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µl of 5’ adapter (10 µM) was added and incubated for 5 min at 65°C before addition of T4 ligase buffer (NEB), final 25% PEG8000 and T4 RNA ligase 1 (NEB ...
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bioRxiv - Genetics 2023Quote: ... 300 ng of PCR products were digested at 37° C for 1 hour with 10 units of MfeI-HF and SspI-HF in 1X rCutsmart buffer (New England Biolabs, cat ...
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bioRxiv - Biochemistry 2023Quote: ... crosslinking of Pex1-AzF was performed after a 10 min 30 deg C incubation in ATP regeneration mix with and without apyrase (NEB#M0398S ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... gDNA (10 µg) was digested overnight at 37°C with 100 U each of the restriction endonucleases MseI (NEB, R0525L) and BglII (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The coverslips are then incubated for 30 min at 37°C with DMEM supplemented with 10% SVF containing 0.12 μM SNAP-Cell TMR-Star (New England Biolabs #S9105S), rinsed twice with PBS and incubated for 45 min with Dapi (1μg/ml) ...
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bioRxiv - Molecular Biology 2021Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 1 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml tris buffer (10 mM Tris HCl pH 8.0) ...
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bioRxiv - Genetics 2019Quote: ... was digested at a concentration of 100 ng / μl overnight in 7 ml in a 15 ml Falcon tube at 37°C with NEB Cutsmart Buffer + 10 units of NotI-HF (New England Biolabs # R3189) per ml ...
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bioRxiv - Cell Biology 2021Quote: ... 20µg of protein lysates and media samples were incubated with glycoprotein denaturation buffer at 95°C for 10 min and treated with 0.3µl Endo H (NEB; Catalog no #P0702) enzyme in glyco-buffer 3 for one hour at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 2 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml tris buffer (10 mM Tris HCl pH 8.0) ...
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bioRxiv - Bioengineering 2019Quote: ... eukaryotic recombinant Stlac2 was denatured with Glycoprotein denaturing buffer at 100 °C for 10 min to be deglycosylated using PNGase F (NEB, USA) according to instructions ...
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bioRxiv - Microbiology 2023Quote: ... The sonicated DNA was end-repaired for 30 minutes at 20°C in 200 μl final volume containing 1x end-repair buffer and 10 μl of end-repair mix (NEB, E6050L). The reaction was cleaned in 2x AmpureXP Beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... 6 μg of digested nucleic acids were treated overnight at 37 °C with or without 10 μl of RNase H (New England BioLabs, M029L) in 1x RNase H buffer and 1/10th of the samples was saved as input ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 1 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml Tris buffer (10 mM Tris HCl (pH 8.0)) ...
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bioRxiv - Biochemistry 2023Quote: ... was used to linearize plasmid (10 µg) by incubating at 37 °C for a minimum of 2 hours in 1x CutSmart buffer (NEB, B7204S). Linearized plasmid was purified by extraction with an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1 ...
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bioRxiv - Genomics 2023Quote: ... The pellet was resuspended in 90 µL of freshly prepared Micro-C “Master Mix 1” (10 μl 10x T4 DNA Ligase Buffer, 75 μl ddH2O, 5 μl T4 PNK (NEB #M0201L)) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells were incubated at 37°C (30°C for NEB stable), 200 min-1 either in 5 ml (for a high copy ...
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bioRxiv - Developmental Biology 2019Quote: ... Total RNA was then treated with DNase for 10 min at 37 °C to remove genomic DNA [10 μg RNA in 100 μl reaction buffer with 2U DNase I from NEB (Ipswich, MA)] followed by phenol-chloroform extraction and ethanol precipitation.
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bioRxiv - Physiology 2022Quote: ... from epithelium or sensory neurons underwent digestion for 10 hours at 37°C with MspI restriction enzyme (New England Biolabs, Ipswich, MA), followed by purification with AMPure XP (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... Adapter ligation was performed at 20°C for 10 min, with Adapter Mix (AMX, ONT) and Blunt/TA Ligase Master Mix (New England BioLabs, cat# M0367). After a final 1X clean-up and washing of the beads with Adapter Binding Buffer (ABB ...
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bioRxiv - Microbiology 2023Quote: ... A total of 3 μg of RNA was denatured at 65°C for 10 min with equal volume of RNA loading dye (New England Biolabs, MA, USA). RNA samples ...
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bioRxiv - Developmental Biology 2024Quote: ... The RNA was then treated with DNase for 10 min at 37◦C to remove genomic DNA [10 μg RNA in 100 μl reaction buffer with 2U DNase I from NEB (Ipswich, MA)] followed by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Genomics 2019Quote: ... the temperature was slowly ramped down (−0.1°C/sec) to 45°C (for Hybridase RNase H) or 22°C (for NEB RNase H), and the mixture was incubated at 45°C (for Hybridase RNase H ...
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bioRxiv - Microbiology 2022Quote: ... Around 100ng of the RNA was kept as input control and stored at -80°C whereas the remaining amount was subjected to immunoprecipitation using m6A specific antibody (NEB #E1610S). Initially ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µ l of cfDNA was incubated at 37°C for 1 hour with the following reaction mixture: NEBuffer™ 2 (NEB, B7202), 0.25 mM MnCl2 (SIGMA ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... The TD-PCR protocol began with an annealing temperature of Tm + 10 °C for the first cycling phase (Tm calculated by NEB Tm Calculator tool). The annealing temperature was then decreased by 1 °C per cycle until the primers’ Tm was reached ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids cleavage was conducted at 37 °C for 10 min and stopped by addition of 2×RNA loading dye (New England Biolabs, Ipswich, MA, USA). Before loading ...
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bioRxiv - Genomics 2023Quote: ... and chromatin was digested during 10 min at 37 °C with 6 Kunitz units of MNase (New England Biolabs, 200 Kunitz units/µl) per 1 million cells ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Membranes were incubated overnight at 4 °C with the appropriate antibody: rabbit monoclonal anti-GSK3β (1:1,000; product # 9315, Cell Signalling Technology, New England BioLabs, Whitby, Ontario, Canada), rabbit polyclonal anti-p[Ser9]GSK3β (1:500 ...
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bioRxiv - Immunology 2021Quote: ... 60sec 72 °C (NEB # E7645S).
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bioRxiv - Cell Biology 2023Quote: ... cells were resuspended in 50 mL buffer 3 (20mM KPi pH 7.4, 1.2M Sorbitol, 10 mM ribonucleoside vanadyl complex (NEB S1402S, pre-warmed at 65 °C), 0.08mg/ml Zymolyase-20T (Amsbio 326921) ...
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bioRxiv - Neuroscience 2020Quote: ... and annealed (denaturation at 95°C for 5 min and then slow cooling of the samples at the rate of -2°C/sec from 95°C to 85°C and then - 0.1°C/sec from 85°C to 25°C for annealing of heteroduplexes) before addition of T7 Endonuclease I (NEB, Ipswich, Massachusetts, USA, M0302S). Heteroduplexes containing small mutations at the intended site should be cleaved into two fragments ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μL GlycoBuffer 2 (10 ×) (NEB), 10 μL 10% NP-40 (NEB) ...