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Citations for New England Biolabs :
51 - 100 of 10000+ citations for DNA Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: ... 25–50 ng of template DNA was added to Polymerase Chain Reactions (PCR) containing 1× Standard Taq Buffer (New England Biolabs), 2.5 mm MgCl (New England Biolabs) ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 ng of the LMNB1 plasmid construct was subjected to a standard mutagenic PCR reaction with Q5 High Fidelity DNA polymerase (M0491, New England Biolabs) and 25 ng of specific primers ...
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bioRxiv - Molecular Biology 2019Quote: ... Amplification of full-length cDNAs of CG33172 (clone MIP10235 in BDGP) was done using standard PCR techniques using Q5 high fidelity DNA Polymerase (New England BioLabs). Amplification products were cloned between the HindIII and NotI restriction sites of the pET-28a (Novagen ...
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bioRxiv - Synthetic Biology 2020Quote: Plasmid cloning was performed primarily using standard PCR and restriction enzyme cloning with Vent DNA Polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Synthetic Biology 2020Quote: Plasmid cloning was performed primarily using standard PCR and restriction enzyme cloning with Vent DNA Polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Molecular Biology 2019Quote: ... amplified cDNA samples were subjected to standard Illumina fragment library preparation using the NEBnext Ultra DNA library preparation chemistry (New England Biolabs). In brief ...
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bioRxiv - Synthetic Biology 2019Quote: Plasmid cloning was performed using standard molecular biology techniques of PCR and restriction enzyme cloning with Phusion DNA Polymerase (NEB), restriction enzymes (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... primers used are listed in Supplementary Table 5 and PCR was performed by standard procedures using Phusion high fidelity DNA polymerase (NEB). Gene deletion of sll1951 was performed by amplifying an upstream 966bp fragment in the N-terminal region of sll1951 using primers Sll1951leftfor and Sll1951leftrev and a 954bp downstream fragment in the N-terminal region of sll1951 using primers Sll1951rightfor and Sll1951rightrev ...
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bioRxiv - Genomics 2022Quote: All plasmids were constructed using standard cloning methods with New England Biolabs restriction enzymes and T4 DNA ligase (New England Biolabs) or with the NEBuilder HiFi DNA Assembly kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... A biotin pull down was then performed on the size-selected fragments using streptavidin-coated beads before library preparation on the beads using the standard NEBNext Ultra II DNA library prep kit for Illumina (NEB) protocol ...
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bioRxiv - Microbiology 2023Quote: ... derived from cloning with primers/restriction enzymes (Table S3) with standard cloning procedures (restriction enzymes and T4 DNA ligase from NEB) as described previously (57) ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmids encoding RBC biosensor proteins were assembled using standard molecular biology techniques of PCR and restriction enzyme cloning with Phusion DNA Polymerase (NEB), restriction enzymes (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation was conducted by the standard ≥100 ng protocol from the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) with minor modifications ...
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bioRxiv - Synthetic Biology 2023Quote: ... All transformations were carried out using standard lithium acetate protocol69 using DNA linearized with PmeI or SwaI (New England Biolabs) to produce genomically integrated cells ...
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bioRxiv - Genetics 2023Quote: ... All plasmids were cloned by Gibson Assembly following the standard NEB Builder HiFi DNA Assembly master mix protocol [New England Bio Labs (NEB), Massachusetts ...
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bioRxiv - Evolutionary Biology 2024Quote: Indexed libraries were generated using the standard Illumina two-step PCR protocol using Q5 high fidelity DNA polymerase (New England Biolabs). Paired-end sequencing with a 2×250 bp read length was performed at the Bio-Environment platform (University of Perpignan Via Domitia Perpignan ...
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bioRxiv - Plant Biology 2023Quote: ... EM-seq libraries were prepared from sheared DNA using an enzymatic methyl-seq kit following the standard instructions (New England BioLabs), and were subjected to NextSeq550 using 75bp paired-end sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... BRSKs were also cloned into a pcDNA3 vector using standard a standard T4-ligase (NEB) protocol and expressed in frame with a 3C-protease cleavable N-terminal tandem STREP-tag ...
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bioRxiv - Genomics 2020Quote: ... The resulting fragment is then ligated into a digested pCDNA3-YFP Basic plasmid that had been cut with Kpn1 and Xho1 using a standard T4 DNA Ligase protocol (NEB #M0202). Once the insert ...
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bioRxiv - Immunology 2019Quote: ... PCR amplification was performed using Taq DNA Polymerase with Standard Taq Buffer according to the manufacturer’s protocol (New England Biolabs, Ipswich, MA). The PCR product was inserted into pCR™ 2.1-TOPO® TA Cloning® plasmid according to the manufacturer’s protocol (Invitrogen™ ThermoFisher Scientific ...
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bioRxiv - Physiology 2019Quote: ... using the Primer3 plugin in Geneious® 6.1.8 (Biomatters Ltd., Auckland, New Zealand) were amplified using standard Taq DNA Polymerase (New England Biolabs, Whitby, ON) following manufacturer-recommended conditions ...
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bioRxiv - Cell Biology 2021Quote: The recovery probe was prepared as follows: A standard 100 µl PCR reaction was prepared containing 10 ng of bacteriophage λ DNA (NEB), 5 units of Taq polymerase (NEB) ...
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bioRxiv - Cancer Biology 2020Quote: ... and reverse (ccagtgagcttcccgttca) primers with Q5 High fidelity DNA polymerase according to the standard protocol described by the manufacturer (New England BioLabs®). The PCR products obtained after 35 cycles were separated through a 1% agarose gel ...
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bioRxiv - Microbiology 2019Quote: ... the full ORF was amplified from pCC2FOS-Mm3 by a standard PCR reaction using the Phusion DNA polymerase (New England BioLabs, Germany) at 72°C annealing temperature ...
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bioRxiv - Cell Biology 2019Quote: Production of new plasmids for this study was accomplished by standard restriction-ligation or NEBuilder HiFi DNA Assembly (New England Biolabs E5520S), typically using Clontech pEGFP-C1 and -N1 backbones or their derivatives ...
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bioRxiv - Synthetic Biology 2021Quote: minD,minE and FtsA genes were amplified by standard polymerase chain reaction (PCR) amplified using Phusion High-Fidelity DNA polymerase (New England Biolabs, USA) as previously reported24,30 ...
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bioRxiv - Cell Biology 2023Quote: ... New plasmids used in this study were generated using standard restriction-ligation or by using NEBuilder HiFi DNA Assembly (New England Biolabs E552OS). HsPIP5K1A ...
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bioRxiv - Bioengineering 2021Quote: ... standard Taq buffer (New England Biolabs), 2 µL of 20 µM of each primer ...
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bioRxiv - Bioengineering 2021Quote: ... Standards (low range ssRNA ladder, NEB) and 10 pmol of unligated tRNA sample were prepared in an equivalent buffer to the first ligation reaction (1X RNA Ligase 2 buffer (NEB) ...
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bioRxiv - Plant Biology 2021Quote: ... PCR using Standard Taq Polymerase (NEB) was performed for 30 cycles of (denaturation ...
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bioRxiv - Microbiology 2022Quote: ... Color Prestained Protein Standard (NEB, P7719) or Precision Plus Protein All Blue Prestained Protein Standards (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... and standard Q5 reaction buffer (NEB). In an Eppendorf Mastercycler EP Gradient S ...
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bioRxiv - Plant Biology 2023Quote: ... standard Taq buffer (New England Biolabs), 0.5 unit of Taq polymerase (New England Biolabs) ...
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bioRxiv - Plant Biology 2020Quote: ... 1 ∼ 1.5 μl of DNA extract was added to 20 μl of PCR reaction mix containing standard PCR buffer and 1 unit of Taq DNA polymerase (Cat # = M0273, New England Biolabs, Beverly, MA). For long-range PCR ...
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bioRxiv - Biochemistry 2022Quote: ... Site-directed mutant libraries were constructed via overlap extension PCR and standard ligation using T4 DNA ligase (New England Biolabs, Beverly, MA). Oligonucleotide primers carrying NNB base triplets were purchased from Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... 1X Standard Taq Reaction Buffer(NEB B9014S) and certain dosage of M13mp18 template according needs ...
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bioRxiv - Biochemistry 2022Quote: ... using a standard New England BioLabs (NEB) heat shock protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1X Standard Taq Reaction Buffer (NEB). The libraries were gel-purified using the E.Z.N.A Gel Extraction Kit (Omega Bio-Tek) ...
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bioRxiv - Microbiology 2023Quote: ... and a standard Phusion polymerase protocol (NEB). Amplified libraries were cleaned up after gel electrophoresis (selected size between 200 bp to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... using a standard Q5 (New England Biolabs) PCR reaction but holding the temperature at 72 degrees for 30’ ...
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bioRxiv - Cancer Biology 2023Quote: ... Illumina Standard library construction (New England Biolabs) and sequencing (Illumina NovaSeq ...
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bioRxiv - Neuroscience 2019Quote: ... Semi-quantitative analysis was conducted using standard PCR amplification (Q5 Hot-Start High-Fidelity DNA polymerase; NEB, Ipswich, MA, USA, and agarose (2%) gel electrophoresis ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl 10X standard buffer (New England Biolabs), 0.5 μl of each primer (10 mM) ...
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bioRxiv - Neuroscience 2020Quote: ... 0.125 µl Standard-Taq polymerase (New England Biolabs), 1 µl template cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... 3μL of 10x Standard Taq Reaction Buffer (NEB), 0.1 μL Taq Polymerase (NEB) ...
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bioRxiv - Cancer Biology 2021Quote: ... Standard PCRs were run using Taq Polymerase (NEB) using primers provided in Supplemental Table 3 ...
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bioRxiv - Microbiology 2022Quote: 5x OneTaq Standard Reaction Buffer (New England Biolabs)
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bioRxiv - Microbiology 2023Quote: ... 1x standard Taq reaction buffer (New England Biolabs), 0.2 mM of each dNTP ...
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bioRxiv - Genetics 2024Quote: ... 1x Standard Taq Buffer (New England Biolabs, Inc.), 200µM each deoxynucleotide triphosphates ...
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bioRxiv - Microbiology 2019Quote: ... The 16S rRNA gene was amplified using 27F 34 and 534R 35 modified for Illumina sequencing following standard protocols Q5® High-Fidelity DNA Polymerase (New England Biolabs, Inc., Ipswich, MA, USA). Following amplification ...