Labshake search
Citations for New England Biolabs :
1 - 50 of 10000+ citations for DNA Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2022Quote: ... DNA standards (ssDNA standards) were amplified from pRnc-gRNA DNA using Phusion High Fidelity DNA Polymerase (New England BioLabs) with the pWP252fluor reverse primer and forward primers annealing to the expected 5’ termini of the cleaved and uncleaved 5’UTR that would yield the desired size products ...
-
bioRxiv - Molecular Biology 2023Quote: ... Taq DNA polymerase with standard buffer (NEB) was used with 25 cycles ...
-
bioRxiv - Synthetic Biology 2024Quote: Standard Q5 high-fidelity DNA polymerase (NEB) PCR conditions and protocols were used for the DNA amplification ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was amplified using standard PCR using LongAmp Taq DNA polymerase (NEB) targeting the CRISPR locus with a 200-500 bp long amplicon ...
-
bioRxiv - Microbiology 2022Quote: ... Other standard cloning reagents include T4 DNA ligase (NEB), restriction enzymes (NEB) ...
-
bioRxiv - Microbiology 2022Quote: ... whereas diagnostic PCR used standard Taq DNA polymerase (NEB).
-
bioRxiv - Synthetic Biology 2024Quote: ... standard ligations were performed using T4 DNA ligase (NEB), while Gibson assembly reactions used the NEBuilder(R ...
-
bioRxiv - Bioengineering 2020Quote: DNA was synthesized using standard Taq polymerase (New England Biolabs). For 2’-U scrambled DNA synthesis ...
-
bioRxiv - Microbiology 2020Quote: ... standard restriction enzymes and T4 DNA Polymerase (New England Biolabs) were used for cloning ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Taq DNA Polymerase with Standard Taq Buffer (New England Biolabs) was used for the amplification of TR under conditions as follows ...
-
bioRxiv - Developmental Biology 2019Quote: ... Standard subcloning protocols and HiFi DNA Assembly Cloning Kit (NEB, E5520S) were used to generate all the DNA plasmids ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2 μg DNA spiked with methylation standards was digested with SmaI endonuclease (NEB) at 25°C for 8 h ...
-
bioRxiv - Microbiology 2019Quote: ... Successful DNA depletion was verified with a standard PCR using Taq-Polymerase (NEB) and primers 21 and 22 binding to the 16S rDNA.
-
bioRxiv - Biophysics 2019Quote: ... A standard PCR was performed using Taq DNA polymerase in ThermoPol buffer (NEB) at an annealing temperature of 60°C and elongation temperature of 68°C ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were constructed by standard restriction ligation methods using T4 DNA ligase (NEB) or using the NEBBuilder assembly kit (NEB) ...
-
bioRxiv - Cell Biology 2023Quote: ... and standard cut-and-paste cloning using T7 DNA ligase (New England Biolabs). NESRev ...
-
bioRxiv - Biochemistry 2022Quote: ... A standard PCR protocol with Phusion® High Fidelity DNA polymerase (New England Biolabs) and primer-specific annealing temperatures was used for the DNA amplification ...
-
bioRxiv - Biochemistry 2023Quote: Cloning was performed using standard molecular biology techniques to assemble Q5 DNA polymerase (NEB) amplified or synthesized (IDT ...
-
bioRxiv - Synthetic Biology 2023Quote: ... the plasmid that expresses Cre-ER was constructed in parts by gBlock synthesis of DNA fragments (Integrated DNA Technologies) and assembled using standard molecular biology techniques including restriction enzyme digest (NEB) to cut the DNA fragments and T4 ligase (Thermo Fisher ...
-
bioRxiv - Microbiology 2019Quote: ... Inserted DNA fragments were prepared using standard PCR protocols using Phusion polymerase (New England Biolabs). The pMCSG53 vector was linearized by cleavage of the cloning site with SspI ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and Q5 High-Fidelity DNA polymerase in a standard buffer (New England Biolabs, Ipswich, USA). Amplicons were purified on MinElute PCR Purification columns (Qiagen ...
-
bioRxiv - Biochemistry 2023Quote: ... Plasmids were constructed using either standard restriction cloning or HiFi DNA assembly (New England Biolabs) and propagated in DH5α E ...
-
bioRxiv - Plant Biology 2023Quote: ... Taq DNA polymerase (1.75 units) with standard Taq buffer (New England Biolabs, Pickering ON, Canada), 0.2 mM dNTPs and 0.2 μM primers (Table 1) ...
-
bioRxiv - Genomics 2019Quote: ... Standard indexed Illumina libraries were prepared using the NEBNext DNA Library Prep kit (New England BioLabs), followed by amplification using KAPA HiFI DNA polymerase (KAPA Biosystems) ...
-
bioRxiv - Bioengineering 2020Quote: ... The PCR amplifications were conducted using Taq DNA Polymerase with Standard Taq buffer (New England Biolabs). The nucleotide sequences for RT-PCR and real-time PCR primers (synthesized by Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2022Quote: ... The polymerase chain reaction (PCR) was performed with “Taq DNA Polymerase with Standard Taq Buffer” (NEB) according to company’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... Standard indexed Illumina libraries were prepared using the NEBNext DNA Library Prep kit (New England BioLabs), followed by amplification using KAPA HiFI DNA polymerase (KAPA Biosystems) ...
-
bioRxiv - Bioengineering 2021Quote: A standard PCR process was performed using Q5 high-fidelity DNA polymerase from NEB (cat. no. M0491S). All PCR primers were purchased from IDT (Supplementary Table S1) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Aliquots from these lysates were used for a PCR (with a Taq DNA polymerase with standard Taq buffer NEB or EconoTaq DNA polymerase Lucigen) with respective gene primers and an 18mer M13F-FAM fluorescent tagged primer (5’-TGTAAAACGACGGCCAGT-3’ ...
-
bioRxiv - Microbiology 2024Quote: ... The molecular weight standards used were unstained broad range protein standards (NEB) and then the gel was run at 100 volts until the dye front was 1 cm from the bottom of the gel and then stained one hour in Coomassie blue stain (50% methanol ...
-
The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... Standard Gibson Assembly (NEB) cloning was used to construct linker variants PaFtsH2H1-link-32 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Both linear DNA fragments were finally used in a standard restriction digest using EcoRI (New England Biolabs, R3101) and NotI (New England Biolabs ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... This fragment was generated by a standard 3-step PCR protocol using Phusion DNA polymerase (New England Biolabs) and then cloned into the XbaI and HindIII sites of pEX18A (Prentki and Krisch ...
-
bioRxiv - Biochemistry 2020Quote: ... PCR was carried out under standard conditions using the Q5® High-Fidelity DNA Polymerase (New England Biolabs). Samples were kept at 98 °C for 30 s (1 cycle) ...
-
bioRxiv - Genetics 2023Quote: ... Semi-quantitative PCR was performed using Taq DNA polymerase with standard Taq buffer (New England BioLabs Cat # M0273S). PCR products were analyzed on 2% Agarose gels with 0.5 ng/L Ethidium bromide using a 1kb Plus DNA Ladder (New England BioLabs Cat # N3200S ...
-
bioRxiv - Synthetic Biology 2023Quote: ... All plasmids were generated using standard cloning procedures and the NEBuilder HiFi DNA assembly system (New England Biolabs).
-
bioRxiv - Synthetic Biology 2024Quote: Plasmid cloning was performed primarily using standard PCR and restriction enzyme cloning with Phusion DNA Polymerase (NEB #M0530L), restriction enzymes (NEB) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 100 ng of DNA template in 25 µl of 1x One Taq Standard Buffer (Biolabs, Ipswich, MA, USA), 0.2 mM dNTPs ...
-
bioRxiv - Systems Biology 2022Quote: ... we performed a standard PCR using all 10μl of eluted DNA with 25μl 2x NEB High-Fidelity master mix (NEB, MO544), 2.5μl of 25μM forward primer (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’) ...
-
bioRxiv - Developmental Biology 2020Quote: ... standard PCR reactions were performed on 1 ul of cDNA using Q5 High-Fidelity DNA Polymerase (New England Biolabs) and each of the variant PCR primer pairs independently ...
-
bioRxiv - Bioengineering 2019Quote: ... The PCR product was inserted into pcDNA3.1 via 5’EcoRI/3’NotI restriction digestion and a standard ligation protocol (T4 DNA Ligase; New England BioLabs) to create pcDNA3.1-Ncadherin ...
-
bioRxiv - Cell Biology 2023Quote: ... All expression plasmids were generated using standard cloning procedures and the NEBuilder HiFi DNA assembly system (New England Biolabs). All PCR amplified sequences were verified by DNA sequencing ...
-
bioRxiv - Microbiology 2019Quote: ... or standard Taq polymerase (NEB) as described by the manufacturer ...
-
bioRxiv - Genomics 2021Quote: ... standard Illumina primers (NEB #E7335S) and sequencing chemistry were used ...
-
bioRxiv - Genomics 2021Quote: Wild-type HAP1 samples were used for standard ChIP-seq library preparation with NEBNext Ultra II DNA Library Prep kit (New England Biolabs), followed by sequencing on NovaSeq 6000 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Riboprobe templates were synthesized from cDNA via standard PCR using opsin specific primers (Table S1) and Q5 High Fidelity DNA polymerase (New England Biolabs). Primers were designed to bind to the coding sequence of target opsins (SWS2B ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Divergent 5’ phosphorylated primers carrying the mutations were used to amplify the whole plasmid and introducer the mutations using the standard Phusion DNA polymerase protocol from NEB. Mutagenesis primers are listed in Supplementary Table 5) ...
-
bioRxiv - Microbiology 2020Quote: ... the V1-V3 region of 16S rRNA genes was amplified from 2 ng of DNA in 50 μl reactions containing the following components: 1x Standard Taq Reaction Buffer (NEB), 3 mM MgCl2 (NEB) ...
-
bioRxiv - Microbiology 2020Quote: ... library prep was conducted by the standard ≥100 ng protocol from the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB). For insertion junction sequencing ...
-
bioRxiv - Immunology 2022Quote: All CER constructs were generated by standard PCR cloning techniques and the PCR products were assembled using the NEBuilder HiFi DNA Assembly (New England Biolabs). CER21 was generated by linking the extracellular and transmembrane domain of human TIM-4 (GenBank™ accession number AAH08988.1 ...