Labshake search
Citations for New England Biolabs :
301 - 350 of 885 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2022Quote: ... reaction was stopped by adding 2X RNA gel-loading buffer (NEB) with urea ...
-
bioRxiv - Molecular Biology 2020Quote: ... Two different concentrations (80 and 160 Gel Units) of MNase (NEB) were used ...
-
bioRxiv - Bioengineering 2020Quote: ... quenched in 6X purple gel loading dye (New England Biolabs Inc.), and subsequently analyzed in 1% agarose gel (Fisher Scientific)1.
-
bioRxiv - Systems Biology 2021Quote: ... The samples were mixed with gel loading dye (purple, 6×) (NEB).
-
bioRxiv - Cell Biology 2021Quote: ... purified using a Monarch gel extraction kit (New England BioLabs T1020L), and sequenced by a commercial company (ACGT ...
-
bioRxiv - Biochemistry 2021Quote: ... The samples were then combined with 6x gel loading dye (NEB) and ran on a 1% ...
-
bioRxiv - Neuroscience 2021Quote: ... Gels were then placed in Proteinase K (New England Biolabs: P8107S) diluted 1:100 (8 U/mL ...
-
bioRxiv - Microbiology 2022Quote: ... DNA fragments were recovered by gel purification columns (New England Biolabs), and the seven fragments were ligated at equal molar ratios with 10,000 units of T4 ligase (New England Biolabs ...
-
bioRxiv - Molecular Biology 2023Quote: ... All fragments were gel purified and ligated using Gibson assembly (NEB), then transformed into stable competent E ...
-
bioRxiv - Molecular Biology 2023Quote: ... 6× NEB™ Purple Gel Loading Dye (no SDS) (NEB, B7025S) was added to samples before separation on a 0.8% agarose/TAE gel in 1xTAE at 30V for 22 hours at 4°C.
-
bioRxiv - Microbiology 2023Quote: ... DNA fragments were recovered by gel purification columns (New England Biolabs), and the seven fragments were ligated at equal molar ratios with 10,000 units of T4 ligase (New England Biolabs ...
-
bioRxiv - Molecular Biology 2024Quote: ... Six microliters of 6x Gel Loading Dye (B7025S, New England Biolabs) and 1.5 μL of 2.5 mg/ml EtBr were added to each sample ...
-
bioRxiv - Molecular Biology 2023Quote: ... Six microliters of 6x gel loading dye (B7025S, New England Biolabs) and 1.5 μl of 2.5 mg/ml EtBr were added to each RNA sample ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4 μl of 6x Gel Loading Dye (B7025S, New England Biolabs) and 1 μl of 2.5 mg/ml EtBr were added to the RNA size markers.
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmids encoding HA-tagged LC3B proteins were generated by replacing the EGFP sequence in the EGFP plasmids with an HA-tag followed by a Tobacco etch virus protease sequence recognition site and a Flag-Tag (Genewiz) using AgeI and HindIII (New England Biolabs). Lipidation-deficient LC3B proteins were generated by mutagenic PCR using Q5 Hot Start High-Fidelity Master Mix (New England Biolabs ...
-
bioRxiv - Microbiology 2019Quote: In vitro transcribed 3X-FLAG tagged manY transcripts – wild-type or Δenh (1 pmol) were translated in vitro using the PURExpress translation kit (NEB). Translation reactions were stopped by adding Laemmli sample buffer (Bio-Rad) ...
-
bioRxiv - Biochemistry 2022Quote: ... trcP or fragments of the trcP ORF were cloned into pHL100’s SmaI site with the 23 nt upstream sequence and the Flag tag sequence on the C-terminal using NEBuilder HiFi DNA Assembly Master Mix (NEB); subsequently ...
-
bioRxiv - Cell Biology 2019Quote: ... the eGFP-FLAG-HA-MLKS2 sequence was PCR amplified with att flanking primers (Table S2) using high fidelity Q5 polymerase (NEB) and cloned into pDONR221 vector by BP cloning (Cat ...
-
bioRxiv - Biochemistry 2019Quote: ... were generated as described previously.23 In vitro transcription/translation of (fMVF-Flag was carried out using the combination of PureExpress (ΔtRNA, Δaa (E6840S)) and PureExpress (Δribosome (E3313S)) kits (NEB) with the following modifications ...
-
bioRxiv - Genetics 2021Quote: ... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
-
bioRxiv - Cancer Biology 2020Quote: The Flag-HA-USP18WT construct was purchased from Addgene (#22572) and the Flag-HA-USP18C64R/C65R construct was generated using the Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) and the primers OL87 ...
-
bioRxiv - Cell Biology 2021Quote: ... A 12xHis tag was cloned into the N-terminus of pcDNA3.1-MICAL1-FLAG using Q5® Site-Directed Mutagenesis Kits (New England BioLabs) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... COS-7 cell lysates from FLAG-ACBD4/5 (mFFAT) expressing cells were treated for 1 h with λPP (New England BioLabs) as described above ...
-
bioRxiv - Cancer Biology 2019Quote: ... was cloned into a pLenti-hygro backbone to create pLenti hygro Myr FLAG AKT1 (CA AKT) using standard Gibson cloning (NEB).
-
bioRxiv - Biophysics 2022Quote: ... The pACEbac1 XSMC1 XSMC3-Halo-Flag and pIDC XRAD21-8xHis were then combined by a Cre recombinase reaction (New England Biolabs). To generate the baculoviruses ...
-
bioRxiv - Cell Biology 2022Quote: ... To generate pcDNA5/FRT-Tg-NLuc plasmids the Tg gene was amplified from the pcDNA5/FRT-Tg-FLAG plasmid and assembled with the NLuc fragment using a HiFi DNA assembly kit (New England BioLabs). To generate the respective mutant construct plasmids for A2234D and C1264R Tg ...
-
bioRxiv - Neuroscience 2022Quote: ... and MATR3(S85C) from pGW1 FLAG-MATR3-EGFP constructs61 using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and flanking primers that eliminated linker sequences and EGFP ...
-
bioRxiv - Microbiology 2022Quote: ... which was assembled with oligo DNA containing Myc or FLAG tag sequence using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). The resultant plasmids were named pcDNA3-C-Myc or pcDNA3-C-FLAG ...
-
bioRxiv - Microbiology 2023Quote: Epitope-tagged constructs using the GST tag or the 3X FLAG tag were generated using the HiFi DNA Assembly cloning kit (NEB). MntS was cloned into pGEX-6-1 and the GST-MntS fusion was subcloned into pKT25c such that the resulting plasmid lacked the T25 region ...
-
bioRxiv - Bioengineering 2023Quote: ... TADs were directly fused to the N-terminus to dCas9 by digesting the FLAG-NLS-MCS-linker-dCas9 plasmid with AgeI (NEB) and then cloning in PCR-amplified TADs using NEBuilder HiFi DNA Assembly ...
-
bioRxiv - Neuroscience 2023Quote: The EEF1A2 CDS was cloned into pcDNA3.1-FLAG (gift from Saunders Lab) using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB) according to the protocol of the manufacturer ...
-
bioRxiv - Biochemistry 2023Quote: ... 250 ng Golden Gate vector (pcDNA3-based carrying a twin Strep-FLAG tag, synthesized by BioCat, Heidelberg, Germany) 2 U BSA HFv2 (NEB), 1 U T4 ligase (NEB ...
-
bioRxiv - Cell Biology 2022Quote: ... All hybrid constructs were tested for expression and cloned into the pcDNA5/FRT/TO-N-FLAG-hBirA* using restriction enzymes: 5’ KpnI (NEB) and 3’ NotI (NEB ...
-
bioRxiv - Cancer Biology 2024Quote: ... Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and the GeneArt Gibson Assembly HiFi Master Mix (Life Technologies ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 30 μg was diluted 1:3 in gel loading buffer (NEB), sonicated ...
-
bioRxiv - Microbiology 2019Quote: ... The Monarch® DNA Gel Extraction Kit (New England BioLabs, Ipswitch, MA) was used to isolate PCR products during extension overlay PCR ...
-
bioRxiv - Molecular Biology 2020Quote: ... PCR products were loaded with 6X purple gel loading dye (B7025, NEB) and electrophoresed alongside a low molecular weight ladder (range 25 to 766 base pairs ...
-
bioRxiv - Microbiology 2020Quote: ... The Monarch® DNA Gel Extraction Kit (New England BioLabs, Ipswitch, MA) was used to isolate PCR products during PCR ...
-
bioRxiv - Genetics 2022Quote: ... Amplified and gel-purified PCR ORFs were XbaI (R0145S, NEB, Ipswich, Massachusetts) and PacI (R0547S ...
-
bioRxiv - Microbiology 2019Quote: ... PCR products were purified using the Monarch DNA Gel Extraction Kit (NEB) and eluted in 20 µl ddH2O ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... and gel electrophoresis with λ DNA-HindIII Digest marker (New England BioLabs). Samples were then sent to Novogene Corporation (Beijing ...
-
bioRxiv - Plant Biology 2019Quote: ... Following agarose gel (cut with XbaI/PstI) (New England Biolabs, Ipswich MA). Gel extractions were performed using the QIAquick Gel Extraction Kit (Qiagen ...
-
bioRxiv - Genomics 2020Quote: ... using gel-extracted DNA as template and Taq polymerase (New England BioLabs). The probe product was cleaned using mini Quick spin columns (Roche ...
-
bioRxiv - Plant Biology 2023Quote: ... using the Monarch® DNA Gel Extraction Kit (New England Biolabs, USA). In vitro transcription of this template was performed using the mMESSAGE mMACHINE T7 kit (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... and 1x SDS Gel Loading Dye (New England BioLabs, cat. no. B7024S) – this treatment displaced MDA5 and LGP2 but not streptavidin from biotinylated RNA ...
-
bioRxiv - Cancer Biology 2023Quote: ... All gel electrophoresis was completed using a 1517bp-100bp ladder (BioLabs, N3231L). To analyze protein cellular protein ...
-
bioRxiv - Biophysics 2022Quote: ... and then quenched with loading buffer (Gel Loading Dye Purple 6X, NEB) supplemented with 20 mM EDTA and 25 μg/ml heparin ...
-
bioRxiv - Microbiology 2023Quote: ... in the presence of 2,000 gel units micrococcal nuclease (New England Biolabs) for 1 hour ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reactions were mixed with Gel Loading Dye Blue (New England BioLabs Inc.) and ran on a 2% agarose gel with 1X SYBR Safe DNA gel stain (Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: ... The gel was then digested with proteinase K (NEB, cat. no. P8107S) at a final concentration of 8 U/ml in digestion buffer (50 mM Tris ...