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Citations for New England Biolabs :
551 - 600 of 885 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Fragments between ∼1 kb and ∼8 kb were size selected by excision with a clean razor blade and DNA was purified from the agarose by a silica column-based gel extraction kit (New England Biolabs, cat#T1020S ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were run on 1% Agarose gel and those with correct heavy and light chain bands were then used for Gibson ligation (New England Biolabs), cloning into IgG expression vectors ...
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bioRxiv - Microbiology 2020Quote: ... The resulting PCR fragment was gel-purified and assembled with the gBlocks fragment using a 2x Gibson master mix (NEB). Gibson assembly was possible due to a 23 bp sequence shared between the PCR fragment and the gBlocks fragment ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 15 µl distilled water and mixed with 10 µl Gel Loading dye 6X (New England BioLabs). The samples were migrated in an 8.5% SDS-polyacrylamide gel at 180 Volts for 120 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Fragments were then gel-purified and subjected into Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs, #E2621) together with XbaI-NotI digested pCDH backbone ...
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bioRxiv - Genetics 2019Quote: ... The amplicons were gel-purified on a 4% agarose gel and assembled with the cut Lenti-gRNA-Puro plasmid described above using an NEBuilder HiFi DNA Assembly kit (NEB). After incubation at 50 °C for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... The YAP-T2A-mCherry sequence was obtained by gel extraction (Cat. # 740609, Machery-Nagel) and inserted into the pCAGeGFP using ligation reaction (Cat. # M0202, New England Biolabs) to produce the pCAG-YAP expression vector ...
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bioRxiv - Pathology 2020Quote: ... DNA was visualized on 2% agarose gel and genotypes were determined based on PCR product length quantified using a DNA ladder (NEB N0556S ...
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bioRxiv - Developmental Biology 2020Quote: ... 300ng of undigested gDNA and from each restriction reaction were loaded in a 1% agarose gel with molecular weight marker (N3232, NEB). 160V current was applied for 30min in a Midigel tank (370000 ...
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bioRxiv - Neuroscience 2021Quote: ... Products were identified with gel electrophoresis using a molecular weight marker (Low molecular weight marker, New England Biolabs, Frankfurt, Germany). As a positive control ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were gel purified and transcribed in vitro using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). The transcribed sgRNAs were purified using the RNeasyTM mini kit (Qiagen).
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bioRxiv - Genetics 2020Quote: ... and gel purified. pNos-Cas9-attB (Ren et al. 2013) was digested with XbaI/AvrII (New England Biolabs R0145, R0174) to remove Cas9 sequences and the backbone fragment was gel purified ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was amplified with Phusion polymerase for 30-36 cycles and resulting PCR products were separated on a standard 1% agarose gel next to a 100 bp ladder (NEB). PCR primers are listed in Supplemental Table 2 ...
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bioRxiv - Immunology 2021Quote: ... CH1 PCR amplified fragments were gel-purified and cloned into restriction enzyme digested pFUSE2ss-CLIg-hK by the Gibson assembly (NEB). The pFUSE2ss-HoleHeavy-hG1 possesses three “hole” mutations (T366S ...
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bioRxiv - Developmental Biology 2020Quote: ... and microRNAs were isolated by size selection on 6% TBE PAGE gels 1mM (Novex) with clean up performed using Monarch PCR and DNA clean up kit (NEB), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... PCR product was either separated on a 2% agarose gel for 3 hours or digested overnight with MwoI (New England Biolabs) and separated on an agarose gel to determine individual fish genotypes ...
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bioRxiv - Microbiology 2022Quote: ... PCRs purified using the Nucleospin gel and PCR clean-up kit were cloned into plasmid backbones digested with restriction enzymes (NEB) using the In-Fusion HD cloning system (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplicon thus obtained is purified from gel and ligated using T4 DNA Ligase (New England Biolabs Inc., MA, USA) and maintained in HAC1-Δ background.
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were verified by agarose gel electrophoresis and purified using the Monarch PCR and DNA Cleanup Kit (New England Biolabs). In vitro transcription was performed using the T7 RiboMAX Express Large Scale RNA Production System (Promega) ...
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bioRxiv - Cancer Biology 2022Quote: ... The remaining nuclei were resuspended in the NSB buffer supplemented with 1 mM CaCl2 and 2000 gel units/ml of MNase (New England Biolabs) and incubated at 37°C for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... 100 ng of each gel-purified PCR products (total of 19) were mixed and digested with BsmBI restriction enzyme (NEB) for 2 h at 55 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The products were analyzed by electrophoresis in a non-denaturing 12% polyacrylamide gel against a 5’-labeled Low Molecular Weight DNA Ladder (New England Biolabs) and quantitated with a phosphorimager (Typhoon FLA 9500 ...
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bioRxiv - Molecular Biology 2022Quote: ... and the gel regions that contain thyA genes with deletions were extracted by using a Monarch DNA gel extract kit (New England Biolabs). 1 ng of extracted PCR products were analyzed by an Agilent High Sensitivity DNA kit on an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Molecular Biology 2022Quote: ... and analyzed by electrophoresis in a non-denaturing 12% polyacrylamide gel with 5’-labeled Low Molecular Weight DNA Ladder (New England Biolabs) run in a parallel lane ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μl of the Phire PCR reaction was verified on a 2% agarose gel with a low molecular weight ladder (N3233S, NEB). 15 μl of PCR products were pooled and purified using PCR Purification Kit (D4013 ...
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bioRxiv - Synthetic Biology 2022Quote: ... A fraction (6 μL) of the eluate was mixed with an equal volume of 6× purple gel loading dye (NEB) and loaded in 1% agarose gel with ethidium bromide ...
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bioRxiv - Neuroscience 2022Quote: ... was amplified by PCR from the same vector and its DNA was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs). DNA segments for Fc regions ...
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bioRxiv - Molecular Biology 2022Quote: ... 3μl of annealed oligonucleotides were ligated with 50ng of gRNA backbone vector (previously cut with BbsI-HF and gel extracted) using Quick Ligation kit (M2200S, NewEngland Biolabs) and transformed into NEB 5-alpha Competent E ...
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bioRxiv - Immunology 2023Quote: ... The pure genomic DNA was separated from any extrachromosomal vaccine DNA by 2D gel electrophoresis and further digested by the AscI restriction Enzyme (R0558S, NEB) since it cuts only DNA propagated in E ...
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bioRxiv - Synthetic Biology 2023Quote: ... was inserted into the gel-purified C-terminal sub-cloning vector digested with EcoRI and KpnI restriction enzymes (New England Biolabs). Reaction products were transformed into chemically competent Top10 Escherichia coli cells and selected on LB-agar plates with carbenicillin at 37 °C static conditions ...
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bioRxiv - Synthetic Biology 2023Quote: ... was inserted into a gel-purified N-terminal sub-cloning vector digested with MluI and KpnI restriction enzymes (New England Biolabs). Cas13 C-terminal fragments were also PCR amplified out of the full Cas13 sequence templates mentioned above to contain a 5′ sequence to code for the KpnI restriction site a start codon ...
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bioRxiv - Developmental Biology 2023Quote: ... The corresponding bands were cut out and pooled all in one tube followed by purification using the Monarch DNA Gel extraction Kit (T1020; NEB) and subsequently repurified using the DNA cleanup kit (T1030 ...
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bioRxiv - Synthetic Biology 2023Quote: Gel electrophoresis was performed using 1 % agarose gels and the ladders used were either Quick-Load 1 kb Extend DNA Ladder (NEB), or Quick-Load Purple 1 kb plus DNA Ladder (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg of cDNA was amplified with Phusion polymerase for 32 cycles and separated on a standard 1% agarose gel together with a 100 bp or 1 kb ladder (New England Biolabs). Ribosomal protein L32 (RpL32 ...
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bioRxiv - Systems Biology 2023Quote: ... the library pool ranging from 135 to 146 bp including the DNA marker were gel purified and quantified by NEBNext Library Quant kit (NEB) using QuantStudio 5 Real-Time PCR System (Thermo Fisher ...
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bioRxiv - Biophysics 2023Quote: ... the mixtures were deproteinized by adding pronase E (4 μg/μl) and 1X purple gel loading dye (New England BioLabs) and incubated at 55°C for 30 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The reaction was initiated with incubating 1 μM Casπ RNP effectors with 20 nM dsDNA target at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X, NEB) supplemented with 20 mM EDTA and 25 μg/ml heparin ...
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bioRxiv - Biophysics 2022Quote: ... 1 μM Casπ RNP effectors were incubated with 20 nM target plasmids at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X, NEB) supplemented with 20 mM EDTA and 25 μg/ml heparin ...
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bioRxiv - Genetics 2023Quote: ... The PCR products were gel-purified and then assembled into a plasmid backbone digested with MluI (New England BioLabs, R3198S) and SpeI (New England BioLabs ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR reaction and digested plasmid were gel purified and used to perform a Gibson Assembly reaction (Cat. #E2611S, New England Biolabs) followed by bacterial transformation according to the manufacturer instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The purity and approximate size of the cloned fragment were assessed by agarose gel electrophoresis after endonuclease digestion simultaneously with BamHI and XbaI (New England Biolabs, in NEBuffer 3.1 at 37° for 1 hour using 1 U of enzyme per 1 μg DNA) ...
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bioRxiv - Microbiology 2023Quote: ... The larger fragment of the vector was gel purified as described above and the two fragments were ligated by T4 ligase (NEB) to generate the pLenti-NLuc1-9_IRES EGFP transfer plasmid ...
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bioRxiv - Biochemistry 2023Quote: ... In-gel digestion was performed with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega, Madison, WI; Trypsin-ultraTM, MS grade NEB) with a final trypsin concentration of 20 ng/µl in 50 mM aqueous ammonium bicarbonate and 5 mM CaCl2 ...
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bioRxiv - Immunology 2024Quote: ... mRNA used for comparison in NAGE gels was produced using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Amplification specificity was assessed by agarose gel electrophoresis and amplicons were subsequently purified with the Monarch PCR & DNA Clean Up Kit (NEB). Resulting DNA concentrations were spectrophotometrically measured by NanoDrop ...
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bioRxiv - Molecular Biology 2023Quote: ... for 30 min and then at 60 V (∼4 V/cm) until the pink loading dye was reached in 6x gel loading dye (B7025S, New England Biolabs), which shows a similar migration speed to that of bromophenol blue ...
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bioRxiv - Biochemistry 2024Quote: ... After 30 mins the samples were placed on ice and 2 μl loading dye (Purple gel loading dye, no SDS, B7025 New England Biolabs) was added prior to loading 12 μl onto a 1.5% 15 x 15 cm 100 ml 0.5X TB agarose gel ...
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bioRxiv - Cell Biology 2024Quote: ... with or without added PKA (50 ng or 500 ng for gel analysis or 50 ng for LC-MS/MS, New England Biolabs) were combined with protein kinase buffer (PK buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-SUZ12 (NEB, 3737), Anti-ASXl1 (Cell Signaling ...
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bioRxiv - Immunology 2019Quote: ... anti-LC3β (NEB, 3868), anti-calnexin (#2433 ...