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Citations for New England Biolabs :
401 - 450 of 5463 citations for 7 CHLOROMETHYL 2 2 DIMETHYL 2 3 DIHYDRO 1 BENZOFURAN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... and 2 mg/mL proteinase K (New England Biolabs). The tissue sections were lysed separately in 75 μL lysis buffer for 2 hours at 55 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 mM of rNTPs (New England Biolabs GmbH (Germany)) ...
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bioRxiv - Cell Biology 2022Quote: ... pellets were resuspended with 1.11× NEBuffer 2 (B7002, NEB) containing 0.1% sodium dodecyl sulfate (SDS) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 µl of T4 DNA Ligase Buffer (NEB M0202), 1 µl of Restriction Enzyme (20 U ...
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bioRxiv - Cell Biology 2023Quote: ... 25 μL NEBNext HiFi 2 × PCR Master mix (NEB) was added ...
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bioRxiv - Genomics 2022Quote: ... 2 µL 400U/µL T4 DNA ligase (NEB, #M0202V), 10 µL 10mM ATP (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 mM MnCl2 (New England Biolabs, NEB). Control treated cells were incubated with a volume of 50% glycerol equivalent to the volume of RNase III added as well as 2 mM MnCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 mM MnCl2 (New England Biolabs, NEB). Control treated cells were incubated with a volume of 50% glycerol equivalent to the volume of RNase III added as well as 2 mM MnCl2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 2 µl of 10X ExoI buffer (NEB, B0293S). The annealing was performed in a thermocycler by gradually decreasing the temperature from 98 °C to 60 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... BamHI (Site 2) or HindIII (Control; New England Biolabs) overnight at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 2 uL dNTP Solution Mix (10 mM stock, NEB), 2 μl Taq DNA ligase (80 U ...
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bioRxiv - Plant Biology 2023Quote: ... 2 and T4 DNA ligase (both New England Biolabs).
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bioRxiv - Genomics 2023Quote: ... and digested with 2 units of β-agarase (NEB) per block (1 hr. ...
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bioRxiv - Biochemistry 2023Quote: ... SNAP-Cell TMR Star (2 mM in DMSO, NEB), and SNAP-Cell Block (2 mM in DMSO ...
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bioRxiv - Biochemistry 2023Quote: ... and SNAP-Cell Block (2 mM in DMSO, NEB) were prepared and stored as aliquots in the dark at –20 °C ...
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bioRxiv - Genomics 2023Quote: ... and digested with 2 units of β-agarase (NEB) (42°C ...
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bioRxiv - Bioengineering 2023Quote: ... 2 µL of PNGase F (New England Biolabs, MA) was added ...
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bioRxiv - Systems Biology 2024Quote: ... 2 μL of 10x Standard Taq Reaction Buffer (NEB), 0.4 μL of dNTPs (2.5 μM) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 units of DNase I (New England Biolabs, M0303S) were added ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µl PNGaseF (P0705L, Glycerol-free, New England Biolabs) was added and incubated for one hour at 57⁰C ...
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bioRxiv - Bioengineering 2023Quote: ... and mRNA Cap 2’-O-methyltransferase (NEB, catalog #M0366S). We then added a 3′-poly(A ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 U T4 RNA ligase 2 (truncated KQ, NEB) and 40 U Murine RNase Inhibitor (NEB ...
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bioRxiv - Genomics 2024Quote: ... 10 µl of 2% BSA (NEB, B9000S, 0.01% final) and 930 µl of nuclease-free water and supplemented with 1U/ml Protector RNase inhibitor (Millipore Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 6,25µl of LongAmp Taq 2✕ Master Mix (NEB), 4,25µl of milliQ water ...
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bioRxiv - Molecular Biology 2023Quote: ... Unincorporated ddRTP molecules were inactivated by adding 1 µL of Buffer 2 (80 mM MgCl2) and 1 µL of 1:1 rSAP (NEB):50% glycerol ...
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bioRxiv - Genomics 2019Quote: ... NEBNext Multiplex Oligos for Illumina (NEB, Set 1; cat. # E7335, NEB, Set 2; cat. # E7500) were used for multiplexing ...
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bioRxiv - Biochemistry 2020Quote: ... pre-tRNA stock was diluted 1 in 2 into RNA loading buffer (New England Biolabs) and separated on a 10% acrylamide urea-TBE denaturing gel ...
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bioRxiv - Synthetic Biology 2019Quote: ... 5 μl of the resulting amplicon were diluted 1:4 in 1x buffer 2 (NEB), followed by denaturation and re-annealing in a nexus GSX1 Mastercycler (Eppendorf ...
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bioRxiv - Cancer Biology 2021Quote: ... for 1 h followed by incubating with 2 μL Proteinase K (New England Biolabs, USA) at 65 °C ...
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bioRxiv - Genomics 2022Quote: ... and NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2 (NEB E7335S & E7500S). In order to increase input DNA above single library limits (1 μg ...
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bioRxiv - Neuroscience 2023Quote: ... PCR amplified Domain 1 and PCR Domain 2 and HiFi DNA assmbley was performed (NEB). Transformation was performed in DH5α cells (REF) ...
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bioRxiv - Genomics 2021Quote: ... 5 μl of 100 ng/μl cDNA diluted from the previous step was combined with 2 μl block mix and 2 μl nuclease free water (NEB, catalog no. AM9937), then the cDNA-block oligo mix was incubated on a thermocycler under the following condition to allow block oligo mix to bind to 5’ and 3’ end of the cDNA molecule ...
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bioRxiv - Genomics 2020Quote: ... 1 mM ATP and 10 mM DTT and with 1 μl (2 U/μl) of RNase H (NEB), 1 μl (3 U/μl ...
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bioRxiv - Molecular Biology 2020Quote: ... Methylated adapters (RRBS-1, RRBS-2; Table 1) were added by ligation using DNA ligase (New England Biolabs). DNA was purified using ratio of 1:1.2 sample to AMPure XP beads ...
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bioRxiv - Developmental Biology 2021Quote: ... The calibrated small RNA samples were ligated to an adenylated and fluorescently labeled 3’ adaptor using T4 RNA ligase 2 truncated KQ (NEB, M0373S) and were subsequently separated on a 12% polyacrylamide UREA gel ...
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bioRxiv - Biochemistry 2022Quote: ... Linearised plasmid was mixed with 2-3-fold excess of the three insert fragments followed by addition of Gibson Assembly® Master Mix (New England Biolabs) and incubation at 50°C for 60 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted RNA was treated with T4 PNK and preadenylated linker was ligated to the 3′ end using T4 RNA Ligase 2 truncated KQ (NEB, M0373L). Linker-ligated footprints were reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.5-1.0 μg of genomic DNA for each sample was heated at 65°C for 2-3 hours prior to digestion with PstI (New England Biolabs, UK). This enzyme has a 6 bp recognition site and leaves a 4 bp overhang ...
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bioRxiv - Cell Biology 2019Quote: ... A preadenylated DNA adaptor sequence was ligated to the 3’-hydroxyl ends of the RNA fragments using T4 RNA Ligase (T4 RNA Ligase 2, truncated K227Q, NEB #M0351S). The ligated RNA product was reverse transcribed using Superscript III and a barcoded primer with sequence complementarity to the adaptor ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted RNA was treated with T4 PNK and preadenylated linker was ligated to the 3’ end using T4 RNA Ligase 2 truncated KQ (NEB, M0373L). Linker-ligated footprints were reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Eluted RNA was treated with T4 PNK and preadenylated linker was ligated to the 3’ end using T4 RNA Ligase 2 truncated KQ (NEB, M0373L).
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bioRxiv - Developmental Biology 2023Quote: ... the tbb-2 3’UTR sequence was amplified from genomic DNA using PCR and primers 5’-tggatgaactatacaaatagatgcaagatcctttcaagca-3’ and 3’-aggttttcaccgtcatcacccgcgaaaaacccatgtaagt-5’ and the vector containing the sequence of pie-1p::his-15::gfp amplified from plasmid containing pie-1p::his-15::gfp::egg-6 3’UTR using PCR and primers 5’-ggtgatgacggtgaaaacct-3’ and 3’-ctatttgtatagttcatccatgcc-5’ were used to generate pie-1p::his-15::gfp::tbb-2 3’UTR by using Gibson assembly (NEB E2611). To add the wild type or mutant 3xmir-51 seed sequences ...
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bioRxiv - Molecular Biology 2023Quote: Internally fluorescein-labelled RNA was produced by ligation of in vitro transcribed 5′ acceptor RNA fragment with chemically produced 3′ donor RNA containing internal fluorescein dT modification and 5′ monophosphate essential for ligase activity using T4 Ligase 2 (NEB #M0239S). Additionally ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted RNA was treated with T4 PNK and preadenylated linker was ligated to the 3’ end using T4 RNA ligase 2 truncated KQ (NEB, M0373L). Linker-ligated RNA was reverse transcribed with Protoscript II (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 pM/μl TrAEL-seq adaptor 1 and 1 μl T4 RNA ligase 2 truncated KQ (NEB M0373L). Plugs were then rinsed with 1 ml tris buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µL of linker ligation mixture was added (38% PEG-8000, 1x NEB T4 ligase buffer, 2 µM oBZ407_preA preadenylated linker, 100 units NEB T4 RNA ligase 2, truncated) and they were further incubated at 37 °C for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... then quenched with 2 μL 6X purple loading dye (NEB) at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µL of 10x uracil DNA glycosylase buffer (NEB M0280S), 2.5 µL RNase A (M0280S ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 units of 2’-O-Me-transferase (New England Biolabs) and 25 units RNasin Plus RNase inhibitor (Promega) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 200 U/uL of T4 RNA Ligase 2 (NEB M0351S), 1X T4 RNA Ligase Reaction Buffer (NEB B0216L) ...