Labshake search
Citations for New England Biolabs :
201 - 250 of 422 citations for 6 Amino m ethylphenol hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... It was subsequently reverse transcribed into cDNA with M-MuLV reverse transcriptase (New England Biolabs M0253S). qRT-PCR was carried out using SYBR Green PCR Master Mix (Applied Biosystems 4368708 ...
-
bioRxiv - Plant Biology 2022Quote: Reverse transcription was performed using 2 µg of total RNA and M-MuLV Reverse Transcriptase (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA of the samples was made following the NEB M-MuLV Reverse Transcriptase protocol (NEB #M0253). Qubit was used to assess the concentration of cDNA in the samples ...
-
bioRxiv - Cell Biology 2023Quote: ... The DNA was then digested using Dpn II (375 U DpnII (NEB, R0543 T/M/L) in 1× Dpn II buffer (NEB ...
-
bioRxiv - Biophysics 2023Quote: ... A total of 1 μg of cDNA was prepared using the M-MulV reverse transcriptase (NEB) as per vendor instructions ...
-
bioRxiv - Immunology 2024Quote: ... cDNA synthesis was performed using ProtoScript M-MuLV First Strand cDNA synthesis kit (New England Biolabs) and random hexamer primers ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA was produced from ∼1 µg of RNA using M-MuLV Reverse Transcriptase (New England Biolabs). RTqPCR was performed on 1:10 dilutions of the cDNA using iTaq Universal SYBR Green Supermix (Bio Rad ...
-
bioRxiv - Microbiology 2019Quote: ... The primer sets used were designed by Primer 6 (PREMIER Biosoft Biolabs) (Appendix Table S2) ...
-
bioRxiv - Biochemistry 2019Quote: ... unlabeled AdoMet was left out and 6 μg of MBP2* protein (NEB) was added to each reaction to increase molecular crowding.
-
bioRxiv - Microbiology 2022Quote: ... the reactions were stopped by adding 2µl of 6× loading dye (NEB) containing 20 mM EDTA and analyzed via agarose gel electrophoresis.
-
bioRxiv - Evolutionary Biology 2020Quote: ... SgRNAs were complexed to 6 μg EnGen Spy Cas9-NLS (NEB #M0646) in an approximately 1:1 molar ratio for 15 min at RT ...
-
bioRxiv - Genetics 2021Quote: ... The extract was bound to 6 ml amylose resin (New England Biolabs) for 1 h batchwise ...
-
bioRxiv - Molecular Biology 2023Quote: ... and TruSeq 6-bp index adaptor ligation by Quick ligase (NEB M2200S), and NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541S) ...
-
bioRxiv - Microbiology 2023Quote: ... the isolated glycoproteins are treated by α-2,3/6/8 neuraminidase (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2019Quote: ... HIV-1 MAG2A-ΔHBR-EGFP was generated from HIV-1 MAG2A-EGFP by deleting amino acids 18-32 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). pEGFP-N1 and pEYFP-N1 were obtained from Clonetech (Mountainview ...
-
bioRxiv - Microbiology 2021Quote: ... The first EcoRI restriction site at 829 bp within the AgeI and KasI restriction sites in RGD4C-AAVP-TNF was deleted in two steps to mutate a thymidine to cytosine nucleotide at position 833 without altering the translated amino acid using the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) by following the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2019Quote: ... according to the manufacturer’s protocols (including the gDNA wipeout step) or M-MuLV reverse transcriptase (NEB, M0253L) according to manufacturer’s standard protocol (using Murine RNase Inhibitor [NEB ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 1 µg RNA was used for each reverse transcriptase reaction using M-MuLV Reverse Transcriptase (NEB) according to manufacturer protocol ...
-
bioRxiv - Evolutionary Biology 2019Quote: RNA was extracted and reverse transcribed using random primers and M-MuLV reverse transcriptase (New England Biolabs) and a PCR product of about 300 nucleotides from a coding sequence was than amplified by PCR using Q5® High-Fidelity DNA polymerase (New England Biolabs) ...
-
bioRxiv - Biochemistry 2020Quote: ... Approximately 200 ng/μl of RNA was used for cDNA synthesis by M-MuLV reverse transcriptase (NEB) and 3′ oligo-dT primer ...
-
bioRxiv - Microbiology 2020Quote: ... The reverse transcription reaction (20 μL) contained 2 μL 10x M-MulV buffer (B0253S, New England Biolabs), 1 μL of 50 μM Oligo 18 dT (SO132 ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 0.25 μL of reverse transcriptase (M-MuLV Reverse Transcriptase kit from New England Biolabs, CAT# M0253S) was prepared and 3.25 μL of this master mix was added to each of the samples for a total volume of 20 μL ...
-
bioRxiv - Genomics 2021Quote: ... 4 μg of total RNA were reverse-transcribed by the M-MLV reverse transcriptase (New England BioLabs) following the manufacturer specifications and using oligo d(T ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3 µl freshly made 1 M NH4HCO3 and calf intestinal alkaline phosphatase (1 U, New England Biolabs) were added ...
-
bioRxiv - Biochemistry 2023Quote: ... M Y229A and MT205D substitutions were generated using the Quick change directed mutagenesis kit (New England Biolabs), as recommended by the manufacturer.
-
bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from extracted RNA samples using M-MuLV reverse transcriptase kit (New England Biolabs Ltd) based on the manufacturer’s instructions and using customized primers splice leader (SL_F ...
-
bioRxiv - Biophysics 2021Quote: ... The SNAP-ligand was covalently attached to the amino-modified staples by mixing the staples and BG-GLA-NHS (NEB, dissolved in DMSO) as previously described (Derr et al ...
-
bioRxiv - Developmental Biology 2019Quote: ... The bound protein (~42kDa, 360 amino acids) was released from the MBP tag by cleavage with Factor Xa (New England Biolabs, Massachusetts, United States) for 24 hours at 4°C in 20mMTris pH 7.2 ...
-
bioRxiv - Plant Biology 2022Quote: ... and 6 µM p-Nitrophenyl Phosphate (pNPP) (New England Biolabs Catalog Number P0757S) at 25°C at a pH of 5.0 ...
-
bioRxiv - Neuroscience 2023Quote: ... The sfGFP1-6-CAM-CB5-mRuby3 were inserted using Gibson assembly (NEB E2611). The insert was amplified with the following primers ...
-
bioRxiv - Microbiology 2023Quote: ... 6 ng of sample was treated with 2 units T4 Polynucleotide Kinase (NEB) and incubated at 37°C for 30 min ...
-
bioRxiv - Plant Biology 2024Quote: ... CLAMT1c-Iso2 (Supplementary table 6) were amplified by Phusion polymerase (New England Biolabs) from cDNA (CLAMT1b ...
-
bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were loaded onto a 6 mL amylose column (New England Biolabs), washed in 50 mM HEPES-KOH (pH 7.5) ...
-
bioRxiv - Plant Biology 2019Quote: ... cDNA synthesis was performed for 1 h at 42°C using M-MuLV reverse transcriptase (200 U, NEB) followed by a heat-inactivation at 80°C for 5 min ...
-
bioRxiv - Genetics 2021Quote: ... 1 μg of RNA was reverse transcribed in cDNA using random hexamers and M-MuLV reverse transcriptase (NEB). qPCRs were assembled with Absolute QPCR ROX Mix (Thermo Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... excess gel was removed and embedded specimen were placed in digestion buffer (1X TAE, 0.5% Triton X-100, 0.8 M guanide HCL) with 8 units/ml Proteinase K (NEB) for 2 hours at 37 °C ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... was generated from pCG1-SARS-2-S (a kind gift from M. Hoffmann9 by PCR (Phusion polymerase (NEB)) using primer pairs ...
-
bioRxiv - Developmental Biology 2019Quote: ... First Strand cDNA was synthesized using the Protoscript M-MuLV First-Strand cDNA Synthesis Kit (New England Biolabs). Equal concentrations of RNA (1ug ...
-
bioRxiv - Genomics 2021Quote: ... The RNA samples were reverse transcribed using random hexamer primers and M-MuLV Reverse Transcriptase (New England Biolabs) and the resulting cDNA subjected to qPCR ...
-
bioRxiv - Bioengineering 2023Quote: ... The RT-RPA was performed at 42°C for 30 min by combining M-MuLV-RT (NEB #M0253L) with TwistAmp Basic (TwistDx #TABAS03KIT) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 μg total RNA was reverse transcribed with 50 ng random hexamers using M-MuLV reverse transcriptase (NEB). 1/20th of the resulting cDNA was used then analyzed by qRT-PCR using GoTaq qPCR master mix (Promega) ...
-
bioRxiv - Genomics 2023Quote: ... The cDNAs were built by reverse transcription of proliferating MuSC mRNAs using M-MuLV Reverse Transcriptase (NEB #M0253L). mRNAs were extracted using NucleoSpin RNA Plus XS kit (Macherey-Nagel #740990.50) ...
-
bioRxiv - Biophysics 2023Quote: ... 500 ng biotinylated λ DNA was incubated at 37°C with 1.6 M S-adenosylmethionine (New England BioLabs) and 20 U CpG methyltransferase M.SssI (New England BioLabs ...
-
bioRxiv - Neuroscience 2023Quote: ... and reversely transcribed into complementary DNA (cDNA) using M-MuLV reverse transcriptase (NEB.M0253S, New Englands BioLabs, Ipswich, MA). qPCR was performed using the Brilliant II SYBR Green QPCR master mix (Agilent Technologies ...
-
bioRxiv - Genetics 2023Quote: ... 1.2 M sorbitol) and resuspended in digestion buffer (Buffer B, 200 mM Vanadyl ribonucleoside complex [VRC from NEB] ...
-
bioRxiv - Molecular Biology 2021Quote: ... the samples were mixed with 6× SDS-free Purple Loading Dye (New England Biolabs) supplemented with SYBR Gold and the cleavage products were resolved by native 1.2% agarose gel electrophoresis ...
-
bioRxiv - Biochemistry 2020Quote: ... 6 units of T4 DNA polymerase and 400 units of T4 DNA ligase (NEB) were then added and the reaction was incubated at 12°C for 1 hour to allow second strand synthesis ...
-
bioRxiv - Genomics 2021Quote: ... The decapped RNA was Recapped with 6 μL Vaccinia Capping Enzyme (VCE) (NEB, #M2080) in 1X VCE reaction buffer (50 mM Tris HCl ...
-
bioRxiv - Plant Biology 2019Quote: ... 6 μg of DNA was digested with restriction enzyme EcoRI (New England Biolabs, USA). Digested DNA was separated on 0.8% agarose gel and blotted onto Hybond-N+ nylonmembrane (Amersham Pharmacia Biotech ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 6 μl primers (10 μM) and 50 μl Q5 high fidelity master mix (NEB). The thermocycling parameters were ...