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Citations for New England Biolabs :
1 - 50 of 422 citations for 6 Amino m ethylphenol hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cDNA was synthesized from 1 μg of total RNA using M-MuLV Reverse Transcriptase and Random Primers 6 (both New England Biolabs) at 42 °C for 60 min and diluted in 1:4 ratio by PCR grade water ...
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bioRxiv - Biochemistry 2020Quote: ... Cross-links were reversed from eluted chromatin by adding 6 μL of 5 M NaCl and 2 μL Proteinase K (NEB; P8107S) and incubation overnight at 65°C ...
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bioRxiv - Biophysics 2023Quote: ... we dilute the stock DNA to a final concentration of 6 mg/mL with 1/10th volume of 10× CutSmart Buffer (0.5 M Potassium Acetate, 0.2 M Tris-acetate, 0.1 M Magnesium Acetate; New England BioLabs), 0.1% Tween (to prevent non-specific interactions) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The resulting library was pooled into 12 separate 60 amino acid long sub-libraries (amino acids 1-60, 61-120 etc.) and combined via Gibson Assembly (NEB) with a linearized p414ADHΔter Hsp90 destination vector ...
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bioRxiv - Genomics 2022Quote: ... The pellet was resuspended in 1 mL of NEB and centrifuged at 11,000rpm for 20 min on a sucrose gradient (0.65 mL of 1.6 M sucrose in NEB, 0.35 mL of 0.8 M sucrose in NEB). The Nuclei pellet was then resuspended in 1 mL of NEB ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei-containing pellets were resuspended in 1 mL of NEB + 0,25 M sucrose and centrifuged at 20000xg for 20 min on sucrose gradient (0.65 mL of 1.6 M sucrose in NEB, 0.35 mL of 0.8 M sucrose in NEB). The pellet was resuspended in 1 mL of NEB and formaldehyde to a final concentration of 1% ...
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bioRxiv - Microbiology 2020Quote: ... and M-MuLV reverse transcriptase (NEB) with buffer ...
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bioRxiv - Genomics 2022Quote: ... 200 μ M GTP (NEB, N0450S)).The run-on reaction was stopped by adding Trizol LS (Life Technologies ...
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bioRxiv - Genetics 2019Quote: ... and Phusion polymerase (M-0530, NEB) was used when DNA fragments were sequenced ...
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bioRxiv - Genomics 2022Quote: ... and Phusion polymerase (M-0530, NEB) was used when DNA fragments were sequenced or used for molecular cloning ...
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bioRxiv - Molecular Biology 2023Quote: ... M-MuLV Reverse Transcriptase (NEB, M0253L), RNase inhibitor (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... M-MLV RT buffer (NEB; B0253S) 1X ...
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bioRxiv - Genomics 2023Quote: ... and Phusion polymerase (M-0530, NEB) was used when DNA fragments were sequenced or used for molecular cloning ...
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bioRxiv - Microbiology 2021Quote: ... and M-MuLV reverse transcriptase (NEB M0253). For quantitative RT-PCR ...
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bioRxiv - Plant Biology 2021Quote: ... M-1 was digested with AflII (NEB) overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 U M-MuLV RT (NEB) in RT Buffer (25 mM KCl ...
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bioRxiv - Molecular Biology 2020Quote: ... M-MuLV reverse transcriptase (New England Biolabs) and oligo-d(T ...
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bioRxiv - Systems Biology 2023Quote: ... and M-MuLV Reverse Transcriptase (NEB M0253). qPCR was performed in biological and technical triplicate using an Applied Biosystems QuantStudio 3 Real-Time PCR System with Fast SYBR Green Master Mix (Thermo Fisher 4385617) ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 and 4 μL of 6× Gel Loading Dye (B7025S, New England Biolabs) and 1.5 and 1 μL of 2.5 mg/mL EtBr were added ...
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bioRxiv - Bioengineering 2020Quote: ... Single amino acid substitutions were introduced utilizing the KLD Enzyme Mix (NEB) following PCR amplification with mutagenic primers (Genewiz) ...
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bioRxiv - Microbiology 2021Quote: ... MeV-M BiFC constructs were made by exchanging NiV-M fused to VN173 or VC155 for MeV-M using the Gibson Assembly Cloning Kit (New England BioLabs). All mutant plasmids were generated using the Q5 site-directed mutagenesis kit using PAGE-purified mutagenesis primers designed using the online NEBase Changer primer design tool (New England BioLabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 μL of M-MuLV reverse transcriptase (NEB), and 1 µL of murine RNase inhibitor (NEB ...
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bioRxiv - Genomics 2022Quote: ... 200 μ M ATP (New England Biolabs (NEB), N0450S) ...
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bioRxiv - Genomics 2022Quote: ... 200 μ M ATP (New England Biolabs (NEB), N0450S) ...
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bioRxiv - Plant Biology 2021Quote: ... M-TM2^2 was digested with BslI (NEB) for 3 hours at 55°C and M-2 was digested with XhoI (NEB ...
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bioRxiv - Microbiology 2021Quote: ... 1X M-MuLV Reverse Transcriptase buffer (NEB, M02553L) and 10 U/mL of M-MuLV Reverse Transcriptase (NEB ...
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bioRxiv - Cancer Biology 2019Quote: ... T4 Pyrimidine dimer glycosylase (T4PDG NEB #M-308S) to remove pyrimidine dimer lesions ...
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bioRxiv - Microbiology 2021Quote: ... and M-MuLV Reverse Transcriptase (New England Biolabs) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and M-MuLV Reverse Transcriptase (cat# M0253S, NEB). qRT-PCR reactions were done using TaqMan® Fast Advanced Master Mix (cat# AB-4444557 ...
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bioRxiv - Bioengineering 2023Quote: XhoI restriction enzyme (NEB cat. no. R0146S/M)
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bioRxiv - Microbiology 2021Quote: ... purple (6×) (New England Biolabs). Samples were loaded into a 4-15% precast polyacrylamide gel ...
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bioRxiv - Molecular Biology 2020Quote: ... Random Primer 6 (NEB, #S1230S), RNasin Ribonuclease inhibitor (Promega ...
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bioRxiv - Genomics 2024Quote: ... and random primer 6 (NEB). The NEBNext Ultra II Non Directional RNA Second Strand Synthesis Module was subsequently used to convert single-stranded cDNA to double-stranded cDNA ...
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bioRxiv - Microbiology 2024Quote: ... 6 U DNase-I (NEB) and 3 µL RNase A (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplifications of rbcL sequences were performed with the RbcL-M-F and RbcL-M-R primers using PCR Ready Mix (BioLabs, Israel), and 50ng DNA-template ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin digestion was initiated by adding 1:10 (trypsin to protein, m/m) mass spectrometry grade trypsin (New England Biolabs #P8101S) in 50 mM ammonium bicarbonate ...
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bioRxiv - Plant Biology 2021Quote: ... Amino acid substitutions were generated with the Q5 site-directed mutagenesis kit (NEB) with specific primers (Supplemental Table 4) ...
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bioRxiv - Immunology 2022Quote: Plasmids containing a cDNA encoding the full-length M segment from SNV (pWRG/SN-M(opt) 67 and pWRG/AND-M(opt2) 68 were used mutagenized using the Q5 Site-Directed Mutagenesis Kit (NEB, Cat# E0554S). Primers were designed using the NEBaseChanger tool (https://nebasechanger.neb.com/) ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of genomic DNA were digested and loaded into each lane. Mouse (M. musculus and M. spretus) genomic DNA was digested with methylation-sensitive (HpyCH4IV, NEB cat. R0619L) or methylation-insensitive (ApoI ...
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bioRxiv - Neuroscience 2021Quote: ... m and f plasmids using HpaI and AccI (NEB). The first linker ...
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bioRxiv - Genomics 2022Quote: ... 0.7 µl T4 ligase (2 M U/ml, NEB), 250 ng DNA (adapters in File S1 ...
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bioRxiv - Plant Biology 2019Quote: ... and 20 units of M-MuLV (New England Biolabs) for 1 h at 42°C and then for 15 min at 70°C ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was incubated with M-MLV reverse transcriptase (NEB) with 10 mM dithiothreitol (DTT) ...
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bioRxiv - Neuroscience 2023Quote: ... 100 U of M-MLV Reverse Transcriptase (NEB; M0253S), M-MLV RT buffer (NEB ...
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bioRxiv - Bioengineering 2023Quote: HindIII-HF restriction enzyme (NEB cat. no. R3104T/M)
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bioRxiv - Cancer Biology 2021Quote: ... 70 mM Tris-Hydrochloride (Tris-HCl, pH 7.6 at 25°C) and 1 U/µl T4 PNK (New England BioLabs). Reaction mixtures were incubated at 37°C for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... 6 μl of 10 mM MnCl and 6 μl (=2400 units) of Lambda phosphatase (#P0753, NEB). Dephosphorylation was carried out at 30°C for 30 minutes and stopped by addition of Roti Load buffer (#K929.1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in spheroblast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 μM beta-mercaptoethanol) and digested with 0.002% 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were resuspended in 1 mL spheroplast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 µM beta-mercaptoethanol), and 1.2–5 µL 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Bioengineering 2022Quote: ... and single amino acids were made by KLD site directed mutagenesis (New England Biolabs). Rotavirus antigens were designed based on optimized sequences described previously ...