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Citations for New England Biolabs :
401 - 450 of 4581 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Luna® Universal qPCR Master mix (New England Biolabs, cat# M3003L) was employed for RT-qPCR according to manufacturer’s recommendations (Initial denaturation 95°C ...
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bioRxiv - Microbiology 2021Quote: ... using Luna® Universal qPCR Master Mix (New England Biolabs, USA) and gene-specific primers (Table S5) ...
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bioRxiv - Microbiology 2022Quote: ... qRT PCR was performed using Luna Universal qPCR master mix (NEB) as per manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... 5 µl of Luna® universal qPCR master mix (#M3003L, NEB), and 3 µl of nuclease-free water ...
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bioRxiv - Microbiology 2022Quote: ... Samples were amplified with Luna Universal qPCR Master Mix (NEB, USA) according to manufacturer’s instructions in a Mx3005 qPCR system (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... Gene expression was determined using Luna Universal qPCR Master Mix (BioLabs) using Roche LightCycler96 system ...
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bioRxiv - Genetics 2023Quote: ... 5μL of 2× Luna Universal qPCR Master Mix (NEB, MA, USA). The amplification program was set as follows ...
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bioRxiv - Physiology 2024Quote: ... and Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA). Targeted genes related to gluconeogenesis and glucose transporter were ...
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bioRxiv - Bioengineering 2023Quote: ... and according to the Luna Universal qPCR Master Mix protocol (NEB). Reaction volumes of 10 μL ...
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bioRxiv - Cell Biology 2023Quote: ... using a universal qPCR master mix (New England Biolabs, Ipswich, MA). All qPCR primer sequences are in Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... using the Luna universal qPCR Master Mix (New England Biolabs, M3003) for cellular genes or the Luna universal probe qPCR Master Mix (New England Biolabs ...
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bioRxiv - Pathology 2024Quote: ... and Luna® Universal qPCR Master Mix (M3003E, New England BioLabs) on a LightCycler 480 II (Roche Diagnostics ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... was combined with forward and reverse primers (Supplementary Table 3) and the Luna Universal qPCR Master Mix master mix (M3003, NEB) containing SYBR green and ROX passive dye to a final 10ul reaction volume ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplification was then carried out in a 25μl reaction volume using 2X Hot Start Taq 2X Master Mix (NEB) according to the manufacturer’s recommendations and using the following primers: from Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2021Quote: ... SYBR green based RT-qPCRs were performed using Luna Universal One-Step RT-qPCR Kit (NEB).
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bioRxiv - Microbiology 2023Quote: ... while the ter region was amplified using the primer pair 3778_ter 1 qPCR fwd with 3778_ter 1 qPCR rev and the Luna Universal qPCR Master Mix (New England Biolabs). Quantification of the ori/ter ratio was performed using the the 2-ΔCT method as described [62].
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bioRxiv - Evolutionary Biology 2019Quote: ... All qPCR reactions were set up with the Luna Universal qPCR master mix (New England BioLabs) and 150 nM of each primer in a total volume of 20 μl ...
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bioRxiv - Microbiology 2019Quote: ... 20 μL qPCR reactions were prepared using Luna® Universal qPCR Master Mix (New England Biolabs) and 2.5 μL of ChIP DNA (1:10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Taqman qPCR was performed using the Luna® Universal qPCR Master Mix (New England Biolabs, M3003) using the C1000 Touch Thermal Cycler (BioRad) ...
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bioRxiv - Biochemistry 2020Quote: ... and qPCR reactions were performed using Luna® Universal qPCR Master Mix (New England Biolabs, USA) according to manufactural instructions ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR reactions were performed using Luna® Universal qPCR Master Mix (New England Biolabs #M3003). Primers for qPCR reactions are shown in Table 1 ...
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bioRxiv - Plant Biology 2022Quote: ... qPCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs Japan, Tokyo, Japan) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed in technical triplicates using the Luna® Universal qPCR Master Mix (NEB) in a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... probe-based qPCR was carried out using Luna Universal Probe qPCR Master Mix (New England Biolabs) and the QuantStudio3 Real-Time PCR System (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR was performed using the OneTaq-Quick-Load 2X Master Mix (New England BioLabs) with Aedes whole-body cDNA as a template ...
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bioRxiv - Molecular Biology 2021Quote: ... We also tested NEB Q5 Hot Start High-fidelity 2x Master Mix (NEB #M0494L), NEB Q5 Hot Start High-fidelity 2x Master Mix plus .01% SDS ...
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bioRxiv - Immunology 2021Quote: ... OneTaq 2x Master Mix with Standard Buffer was used (New England BioLabs, Ipswich, MA). As a template in the first round ...
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bioRxiv - Immunology 2021Quote: ... OneTaq 2x Master Mix with Standard Buffer was used (New England BioLabs, Ipswich, MA). As a template in the first round ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was carried out using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: Darwin Assembly master mix (2x): 0.05 U/µl Q5 High-Fidelity DNA polymerase (NEB), 8 U/µl Taq DNA ligase (NEB) ...
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bioRxiv - Genomics 2020Quote: ... Libraries were amplified using NEBNext® High-Fidelity 2X PCR Master Mix (#M0541S, NEB) with primers Ad1 ...
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bioRxiv - Immunology 2021Quote: ... 25 μl NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, Cat. #M0541L) per reaction and amplified via a 6-cycle PCR program (1 cycle of 72°C for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Three rounds of nested PCR using Phusion HF 2x Master Mix (New England Biolabs) were used to obtain highly specific amplicons for the UNC13A cryptic ...
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bioRxiv - Microbiology 2021Quote: ... Secondary amplification reactions were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB) in 50 µL reaction (26 µL of 2X Mix ...
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bioRxiv - Microbiology 2021Quote: ... 10 μL of WarmStart Colorimetric Lamp 2X Master Mix (M1800) (New England Biolabs, USA), 5 μL of DNAse ...
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bioRxiv - Microbiology 2020Quote: ... PCRs were amplified using Q5® High fidelity DNA polymerase 2X master mix (NEB), 25 pmol of specific ultramers and 20 ng of DNA template ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Colony PCR was performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) and the hACE2 insert primers to identify a positive colony ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR indexing was performed using NEBNext High-Fidelity 2x PCR Master Mix (M0541S, NEB) and sequenced using a NextSeq platform (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: ... amplicons were generated using Q5 Hot Start High-Fidelity 2x Master Mix (M0494, NEB). Amplicons for long-read sequencing were generated with Q5 High-Fidelity polymerase (M0492S ...
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bioRxiv - Microbiology 2021Quote: ... 7.5 μl of OneTaq Quick-Load 2X master mix (New England Biolabs, cat#M0486), and milliQ water to 15 μl ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of 2X Blunt/TA Ligase master mix (New England Biolabs, cat#M0367S), and milliQ water up to 10 μl total volume ...
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bioRxiv - Plant Biology 2021Quote: ... PCR were performed with Quick-Load® Taq 2x Master Mix (New England BioLabs) according to the manufacturer recommendations with the primer displayed in Table S3 ...
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bioRxiv - Cell Biology 2021Quote: ... the purified DNA and the NEBNext® High-Fidelity 2X PCR Master Mix (NEB). PCR reactions were pooled and purified using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Genetics 2022Quote: ... NEBNext® High-Fidelity 2X PCR Master Mix (New England BioLabs, catalog number M0541) and SybrGreen were used to assess the expression level of the Q gene on chromosome 5A ...
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bioRxiv - Synthetic Biology 2022Quote: Q5 High Fidelity 2X Master Mix (New England Biolabs GmbH [NEB], Germany, No. M0492S) was used to perform DNA amplification ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using Hot Start Taq 2X Master Mix (New England Biolabs, USA) in a 20 μL reaction mixture containing 0.25 μM of each primer ...
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bioRxiv - Bioengineering 2019Quote: ... Full-length oligonucleotides were PCR-amplified using Q5 High-Fidelity 2X Master Mix (NEB), size-selected using a 3% agarose EGel EX (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were amplified by PCR using NEBNext High-Fidelity 2x PCR Master Mix (NEB) using PCR primers Ad1_noMX and Ad2_Barcode (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2020Quote: ... Vector PCRs were performed using Q5 High-Fidelity 2X Master mix (New England Biolabs). All GFP related libraries were cloned using BsaI sites ...
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bioRxiv - Cell Biology 2021Quote: ... cDNAs were subjected to end-point PCR using OneTaq® 2X Master Mix (NEB) or quantitative PCR (qPCR ...