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Citations for New England Biolabs :
601 - 650 of 4581 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... PCR reactions were prepared using Q5 High-Fidelity 2X Master Mix (New England BioLabs, Ipswich, MA) and 10 ng genomic DNA in a final volume of 25 μl ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... 10 ng of genomic DNA was amplified using LongAmp Taq 2X Master Mix (New England BioLabs) and the provided barcoded 16S primers ...
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bioRxiv - Microbiology 2019Quote: ... DNA fragments were PCR amplified using Q5® Hot Start High-Fidelity 2X Master Mix (NEB) and oligonucleotides described in Table S1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 µl were used for amplification with Taq 2X Master Mix (New England Biolabs, Ipswitch, USA) using a 20-cycles PCR protocol ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs). PCR product was run on an agarose gel and genotype was determined on size (168bp ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was amplified by PCR using Q5 High-Fidelity 2X Master Mix (NEB Cat. #: M0492) for 20 cycles ...
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bioRxiv - Genomics 2019Quote: ... Libraries were amplified for 12 cycles using NEBNext® High-Fidelity 2X PCR Master Mix (NEB) in total volume of 25µl in the presence of 800nM of barcoded primers (400nM each ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μl reaction mixture composed by 10 μl of Phusion High-Fidelity Master Mix 2X (NEB), 2 μl of Betaine 5M (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... 1 uL of cDNA or gDNA was amplified using Q5 High Fidelity 2X Master Mix (NEB). An indexing PCR was performed next using Nextera XT index kit primers (Illumina ...
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bioRxiv - Synthetic Biology 2021Quote: ... Genomic PCR was performed using Q5® High-Fidelity 2X Master Mix (New England Biolabs, M0492L) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2021Quote: ... and libraries were generated by PCR with NEBNext High Fidelity 2X PCR Master Mix (NEB M0541). Library size was confirmed on a BioAnalyzer prior to sequencing on the NextSeq 550 (40bp read length ...
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Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinizationbioRxiv - Neuroscience 2020Quote: ... Transposed DNA was amplified with barcoded primers in NEBNext High Fidelity 2X PCR Master Mix (NEB) and purified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2019Quote: ... The PCR reaction mixture contained 25 µL of 2X Taq polymerase master mix (New England Biolabs, MA ...
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bioRxiv - Synthetic Biology 2022Quote: All amplifications were made using a high-fidelity polymerase (Q5 2X Master Mix, New England Biolabs) according to the manufacturers’ recommendations in a 25 µl final reaction volume.
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bioRxiv - Molecular Biology 2022Quote: ... Site-directed mutagenesis was performed by PCR with NEBNext High-Fidelity 2x PCR master mix (NEB) using overlapping primers with 15 nt overhangs carrying the desired mutation (IDT).
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bioRxiv - Immunology 2022Quote: ... 5 cycles of pre-amplification were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB) with indexed primers (Ad1 and Ad2) ...
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bioRxiv - Developmental Biology 2022Quote: ... Transposed DNA was amplified with NEBNext High-Fidelity 2X PCR Master mix (New England Biolabs, M0541) as follows ...
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bioRxiv - Biophysics 2022Quote: ... The DiCas7-11 mutants were prepared by Q5 2X master mix mutagenesis kit (New England Biolabs) using primers listed in Supplementary Table 2 and purified similarly as the wild-type DiCas7-11.
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Fragments were amplified using the using Q5® High-Fidelity 2X Master Mix (New England Biolabs) and the following primers ...
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bioRxiv - Genetics 2022Quote: The unc-5 locus was amplified for sequencing by PCR using OneTaq 2x Master Mix (NEB) with primers DLO1081 (5’-TCTTTTCAGGCTTTGGCACTG-3’ ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were prepared using the NEBNext 2x High-Fidelity Master Mix (NEB, Cat. No. M0541L) with amplification primers that were partially complementary to the lentiviral gRNA backbone with overhangs introducing the Illumina P5/P7 adapter sequences ...
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bioRxiv - Synthetic Biology 2020Quote: DNA amplification was done via PCR using Q5 Hot Start HiFi 2X Master Mix (NEB, M0494). All plasmids were constructed using either blunt end ligation or Gibson Assembly ...
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bioRxiv - Synthetic Biology 2019Quote: ... Polymerase chain reactions (PCRs) were conducted using Q5 High-Fidelity 2x Master Mix (New England Biolabs) in the presence of 0.5 µM of forward and reverse primers in a volume of 25 µL ...
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bioRxiv - Synthetic Biology 2019Quote: ... KAPA HiFi HotStart ReadyMix (KAPA Biosystems, or Q5 High-Fidelity 2X Master Mix (New England Biolabs), 60 nt primer sequences containing 20 nt amplification primer sequences and 40 nt ITRs (to be used during bulk suppression PCR) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA fragments were PCR amplified using Q5® Hot Start High-Fidelity 2X Master Mix (NEB) and oligonucleotides described in Supplementary Table 2 ...
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bioRxiv - Genomics 2019Quote: ... 20 ng of the forward backbone was amplified with Len_lib_linF and Len_lib_linR (Supplemental Table 8) using NEBNext High-Fidelity 2X PCR Master Mix (NEB); the minimal promoter and GFP was amplified from 10 ng of the pLS-mP plasmid using minGFP_Len_HAF and minGFP_Len_HAR (Supplemental Table 8) ...
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bioRxiv - Immunology 2020Quote: ... DNA was added to Quick-Load Taq 2x Master Mix (New England Biolabs, Ipswich, MA, USA) and respective primers.
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bioRxiv - Genomics 2021Quote: ... Each well contained 50 µL of NEBNext High Fidelity 2X PCR Master Mix (New England Biolabs), 0.5 µL of each primer at 100 µM ...
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bioRxiv - Genomics 2021Quote: ... Beads were resuspended in 50 μL PCR master mix (25 μL 2X Phusion HF buffer (NEB), 1 μL 12.5 μM Nextera Ad1 primer (Illumina) ...
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bioRxiv - Genetics 2021Quote: ... The transposed DNA was converted into libraries using NEBNext High Fidelity 2x PCR Master Mix (NEB) and the Nextera Index Kit (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... PCR reactions were conducted using Q5® Hot Start High-Fidelity 2X Master Mix (NEB, M0494L). Ligations were conducted using isothermal assembly with NEBuilder® HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Genomics 2021Quote: ... We used the High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA, Cat #M0492L) and the fragments’ optimal annealing temperatures (Supplementary Table 1) ...
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bioRxiv - Genomics 2021Quote: ... and the purified DNA was amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB) with 10 cycles of PCR ...
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bioRxiv - Genomics 2021Quote: ... previously purified ATAC-DNA was amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB) with 10 cycles of PCR ...
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bioRxiv - Genomics 2022Quote: ... 2 μL of 10 mM i5 primer and 25 μL of 2x NEBNext Master Mix (NEB). The following PCR conditions were used ...
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bioRxiv - Microbiology 2022Quote: ... The three fragments were cloned into the pMBsacB plasmid using 2x Hifi Master Mix (M5520AA; NEB) according to manufacturer’s instructions creating the plasmid pLT001.
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bioRxiv - Microbiology 2022Quote: ... 5 μl of the cDNA was mixed with LongAmp Taq 2X Master Mix (New England Biolabs) and 100 pmol Sol-PrimerB (5’-GTTTCCCACTGGAGGATA-3’ ...
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bioRxiv - Plant Biology 2022Quote: ... The OneTaq Hot Start Quick-load 2x Master Mix with GC buffer (M0485L, New England BioLabs) was used for PCR ...
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bioRxiv - Molecular Biology 2023Quote: Purified samples were combined with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs M01541) for amplification ...
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bioRxiv - Molecular Biology 2022Quote: ... Post-capture PCR was performed with the NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541S) for 14-20 cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... we used the 2X Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs), and 300 ng of the cDNA and 10 µM of each the RSV5U and RTRHC primers ...
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bioRxiv - Microbiology 2023Quote: ... Infectious RNA was generated by in vitro transcription using the ARCA 2X T7 master mix (NEB) with subsequent transfection in 293T cells (ATCC® CRL-3216™ ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... Each variant was amplified with primers PK421&422 using Q5 High-Fidelity 2X Master Mix (NEB) in a 200 µl reaction followed by DpnI digestion for 2 h ...
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bioRxiv - Immunology 2023Quote: ... and 100-200ng were PCR amplified using Q5 Hot Start High Fidelity 2x Master Mix (NEB) and 10µM forward/reverse primers flanking the region of interest ...
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bioRxiv - Bioengineering 2023Quote: ... In brief, DNA fragments were amplified by PCR (Q5 2x Master Mix, New England Biolabs (NEB)) ...
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bioRxiv - Molecular Biology 2023Quote: ... pDEST17 was linearized by high fidelity PCR (Q5 High-Fidelity 2x Master Mix, NEB, Cat #M0492), during which sequence encoding the 6x His tag was removed ...
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bioRxiv - Genomics 2023Quote: ... and library amplification was performed using NEBNext® High-Fidelity 2X PCR Master Mix (M0541L-NEB) with Nextera Ad1_noMX and Ad2.X primers ...
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bioRxiv - Cell Biology 2023Quote: ... ORFs for each construct were amplified using Q5 Hot Start High-Fidelity 2X Master Mix (NEB) and subcloned into linear vectors using NEBuilder HiFi DNA Assembly Kit (NEB)
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bioRxiv - Molecular Biology 2023Quote: ... Synthesized cDNA was then amplified using Phusion Hot Start Flex 2X Master Mix (New England Biolabs) and the following primers ...
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bioRxiv - Genomics 2022Quote: ... Barcodes were amplified from the library using Q5 High-Fidelity 2X Master Mix (Q5, NEB #M0492) using primers GWLP P2-3 with 20 cycles ...