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Citations for New England Biolabs :
4751 - 4800 of 9843 citations for Swine IL 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Fragments were cloned into HpaI-digested pMSCVpuro using the Gibson Assembly Cloning Kit (New England Biolabs, E5510S). Plasmids were transformed into E ...
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bioRxiv - Synthetic Biology 2022Quote: ... whole genome sequencing libraries were generated using the NEBNext Ultra II FS DNA Library Prep Kit (NEB) and sequenced using an Illumina NextSeq 500/550 High Output Kit v2.5 (75 Cycles) ...
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bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... and used for in-vitro transcription using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Finally ...
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bioRxiv - Microbiology 2024Quote: ... DNA libraries were constructed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... A repair template plasmid was constructed using Gibson assemble (Gibson et al., 2008) (NEB Hifi assembly kit). The repair construct was made by joining the PfAMA1 coding region and ∼1.5 kb 5’ to the transcription start site amplified from 3D7 genomic DNA ...
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bioRxiv - Microbiology 2024Quote: ... libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and library prep were performed in parallel using the Ultra II Directional RNA-Seq Kit (NEB E7760) and reads were sequenced 2x50bp on an Illumina NovaSeq S4.
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bioRxiv - Biochemistry 2024Quote: ... The inserts were each ligated with digested pmirGLO plasmids using a quick ligasation kit (New England Biolabs) and transformed into JM109 competent cells (Promega) ...
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bioRxiv - Genomics 2023Quote: ... DNase-seq libraries were prepared using the NEBNext Ultra Library Prep Kit for Illumina (New England Biolabs). Illumina sequencing was performed on an Illumina HiSeq instrument to a depth of 12 to 33 million mapped 40-50 bp single-end sequence reads per sample ...
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bioRxiv - Microbiology 2023Quote: ... and used to prepare the library with the Next ultra II DNA library prep kit (E7645, NEB) using 1 μg of DNA per sample as input ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments were assembled into the pBAD24 vector using HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA) with 1-hour ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated from ∼2×106 cells using the Monarch DNA purification Kit (New England Biolabs). For genotyping ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were then isolated using the Monarch® Plasmid Miniprep Kit (New England Biolabs, Ipswich, MA, USA) and used to transfect HEK293T cells (ECACC ...
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bioRxiv - Cell Biology 2024Quote: ... The ChIP libraries were constructed utilizing NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645L) and sequenced on an Illumina NovaSeq 6000 PE150 platform using the 150-bp pair-end sequencing parameters (Novogene ...
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bioRxiv - Cell Biology 2024Quote: ... RNA libraries were constructed utilizing the NEBNext® Ultra RNA Library Prep Kit for Illumina (NEB, E7530L) and sequenced on an Illumina NovaSeq 6000 PE150 platform using the 150-bp pair-end sequencing parameters (Novogene ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 uL RNase A and 1 uL ProtK all from the Monarch gDNA extraction kit (NEB, T3010). gDNA was extracted following the extraction kit’s protocol with the exception that 600 uL of gDNA binding buffer were added to 400 uL quenched reaction and added to the extraction column on two spins of 2 minutes at 1000g ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was isolated from the cells using Monarch® Genomic DNA Purification Kit (New England Biolabs). DNA concentration was measured with Nanodrop One/OneC spectrophotometer (Thermo Fisher) ...
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bioRxiv - Plant Biology 2024Quote: ... Library preparations were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs; NEB). Libraries with different indices were multiplexed and sequenced on an Illumina HiSeq platform (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... Library preparations were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs; NEB). Libraries with different indices were multiplexed and sequenced on an Illumina HiSeq platform (Illumina ...
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bioRxiv - Immunology 2024Quote: ... cDNA was used to make libraries with the NEBNext Ultra II FS Library Preparation Kit (E6177S, NEB), using a five-fold lower reaction volume than instructed by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... 1.4 μM of each purified mRNA was used in PURExpress in vitro Protein Synthesis Kit (NEB#E6800) in 10 μl reaction in 384 well Small Volume black plate (Grenier 788 096) ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were then indexed using the NEBNext Dual Index Kit (E7600S; New England Biolabs, Ipswitch, MA). Final cDNA libraries were analyzed by DNA ScreenTape Analysis on an Agilent 4200 TapeStation per the manufacturer’s protocol to determine library size ...
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bioRxiv - Molecular Biology 2024Quote: cDNA libraries for sequencing were constructed using TruSeq RNA Library Prep Kits for Illumina® (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was then extracted and purified utilizing the Monarch Total RNA Isolation Kit (New England Biolabs), followed by triple DNase I (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... and effectors were cloned using the Gibson Cloning Assembly Kit (New England BioLabs, catalog no. NEB-E5510S). After cloning and sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... Whole cells were processed with the “NEBNext Single Cell/Low Input RNA Library Prep” kit (NEB #E6420) by following manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: Suitable primer pairs (Table S5) were designed utilizing the Q5 site-directed mutagenesis kit (New England Biolabs) to generate mutated amidases ...
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Neuroscience 2024Quote: ... and effectors were cloned using the Gibson Cloning Assembly Kit (New England BioLabs, catalog no. NEB-E5510S). After cloning and sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... and libraries prepared using the NEBNext UltraII Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified amplicons was quantified by qPCR using the NEBNext Illumina Quant kit (NEB). The forward and reverse reads were demultiplexed and fused barcodes were quantified through alignment against custom barcode and primer sequences using Bowtie2 (V2.3.4.1 ...
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bioRxiv - Developmental Biology 2024Quote: ... Ribosomal RNA was depleted from the total RNA using the NEBNext rRNA Depletion kit (New England Biolabs), followed by the conversion of the remaining RNA into an Illumina sequencing library using the NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs) ...
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bioRxiv - Immunology 2024Quote: ... DNA library construction was performed using the NEBNext Ultra II DNA Library Prep Kit from NEB (E7645L). The libraries were sequenced on Illumina NovaSeq 6000 system (50 bp paired-end ...
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bioRxiv - Developmental Biology 2024Quote: ... After PCR amplification or plasmid digestion product was purified with Monarch PCR clean-up kit (NEB, #T1030s) and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription reactions were performed using a LunaScript RT SuperMix Kit (#E3010L, New England Biolabs, Ipswich, MA) with 500 ng of total RNA ...
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bioRxiv - Cell Biology 2024Quote: ... The synthesized probes were then purified using the Monarch® PCR & DNA Cleanup Kit (New England BioLabs) according to the manufacturer’s instructions and the quality was verified on denaturing 12-16% polyacrylamide gels.
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions with the following specifications and modifications ...
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bioRxiv - Cell Biology 2024Quote: The tricistronic Gs and Gs-CFP plasmids were created using NEBuilder HiFi DNA assembly cloning kit (NEB). Gαs (or Gαs-CFP54) ...
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bioRxiv - Biochemistry 2023Quote: ... Indexed DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, Cat # E7645S) with the following specifications ...
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bioRxiv - Immunology 2023Quote: ... in vitro transcription was performed over-night with the T7 High Yield RNA polymerase IVT kit (NEB) for 37°C overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... Quick Blunting Kit and the T4 DNA Ligase enzyme were purchased from New England BioLabs (NEB, Germany). The plasmid extraction kit was purchased from Qiagen GmbH (Hilden ...
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bioRxiv - Genetics 2023Quote: ... RNA library preparation was done using a NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) on a Bravo WS automation system (Agilent) ...
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bioRxiv - Genomics 2023Quote: Libraries were built on beads using the NEBNext Ultra II DNA Library Prep kit (NEB, Cat # E7645L). Biotin pull down was initiated py pipetting 20 μL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... 2 μg of total RNA was converted into cDNA with LunaScript RT SuperMix kit (New England Biolabs). The cDNAs were used as templates for real-time PCR and ran on StepOnePlus real-time PCR system (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Inc.) at Novogene and sequenced on an Illumina NovaSeq 6000 (2 × 150 bp ...
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bioRxiv - Genomics 2023Quote: ... An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor ...
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bioRxiv - Genetics 2023Quote: ... 1x106 HEK293T cells were collected and their gDNA was extracted using the NEB Monarch kit (T3010S, NEB) according to the manufacturer’s protocol ...
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HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were prepared using a NEBNext Ultra II RNA Library Kit for Illumina (New England Biolabs) with the NEBNext Poly (A ...