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Citations for New England Biolabs :
401 - 450 of 1156 citations for c Myc Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... periplasmic expression vectors with C-terminal HA-His6 tags using Gibson cloning (New England Biolabs). Nanobodies were produced in Escherichia coli WK6 transformed with the respective expression vectors ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C for 30 min and dephosphorylated by 1U of Shrimp Alkaline Phosphatase (NEB) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... C or E antibodies and the immunoprecipitates were treated with PNGase F (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2019Quote: ... per 1µg protein for 1 hour at 30°C in PMP buffer (New England Biolabs) plus 1mM MnCl2 ...
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bioRxiv - Genetics 2020Quote: ... Phusion DNA polymerase with annealing at 63°C was used according the manufacturer’s instructions (NEB). Both products were sequenced with DG1328-DG1333 using Sanger sequencing performed by the Australian Genome Research Facility.
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bioRxiv - Microbiology 2019Quote: ... 72°C for 60 seconds) in 50 µl reactions using Phusion PCR polymerase mix (NEB) containing 25 pmol each of the following ultramers (the protospacer-matching sequence is underlined) ...
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bioRxiv - Biochemistry 2021Quote: C-terminally (His)6-tagged LukE was expressed in BL21 (DE3) Escherichia coli cells (NEB). Transformed cells were grown at 37 °C in Terrific broth supplemented with 100 μg/mL ampicillin to a density of OD600 = 0.6 ...
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bioRxiv - Molecular Biology 2021Quote: ... digested genomic DNA by O/N incubation at 16°C with T4 DNA ligase (NEB). Subsequently ...
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bioRxiv - Biochemistry 2021Quote: ... acylated dasTMPRSS2 was treated with 50 U PNGase F (NEB; 37 °C for 45 min) to trim N-glycan branches ...
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bioRxiv - Developmental Biology 2021Quote: ... and the fragments were ligated over night at 4°C with T4 DNA Ligase (NEB) and heat shock transformed into DH5α competent E ...
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bioRxiv - Immunology 2020Quote: ... transferred to 42°C and digested by immediate addition of 2ul of beta-agaraseI (NEB) per 300ul of melted gel and incubation at 42°C for 1h ...
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bioRxiv - Immunology 2022Quote: ... cells were digested with Micrococcal Nuclease at 37°C for 40 min (New England BioLabs) and added 10 mM EDTA to stop the reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... for 30 minutes at 37°C in 1X rCutSmart Buffer (New England Biolabs, cat# B6004S), followed by Trizol extraction ...
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bioRxiv - Molecular Biology 2022Quote: ... and ligated over night at 16°C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Immunology 2023Quote: In situ Hi-C experiments were performed as previously described2 using restriction enzyme MboI (NEB) with minor modifications ...
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bioRxiv - Plant Biology 2022Quote: ... The digestion buffer (25mM triethylammonium bicarbonate (TEAB)) contained the Lys-C protease (P8109S, NEB, Australia) at a 1:20 protease to protein ratio ...
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bioRxiv - Biophysics 2023Quote: ... and BG-oligonuculeotides were labeled with myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer ...
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bioRxiv - Biochemistry 2023Quote: ... reactions were conducted at 37 °C using the PURExpress System (New England Biolabs, Ipswich, MA) 111 in the absence or presence of increasing concentrations of ABCF protein ...
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bioRxiv - Cancer Biology 2023Quote: ... A negative control treated for 4 hours at 37 °C with RNaseH1 (New England Biolabs) was included for each condition ...
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bioRxiv - Cancer Biology 2023Quote: ... digested overnight at 37°C with 1 μl 20 U/μl NotI-HF (NEB R3189S) and 1 μl 10 U/μl PmeI (NEB R0560S ...
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bioRxiv - Plant Biology 2023Quote: ... Then we fused GFP to the C terminus of GUS through seamless cloning (NEBuilder, NEB) using primers KT1101 ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated overnight at 25°C in 100 μl 1x T4 RNA ligase buffer (NEB) containing 40 μl 50% PEG 8000 ...
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bioRxiv - Biophysics 2023Quote: ... followed by heat inactivation for 20 min at 80°C (Quick CIP, New England Biolabs). We added the 5’-phospho group on the synthetic parS fragment by adding a T4 kinase for 30 min at 37°C and heat-inactivated 20 min at 65°C in 1x PNK buffer supplemented with 1 mM ATP (T4 PNK ...
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bioRxiv - Molecular Biology 2023Quote: ... Successful reactions were directly incubated at 37 °C with 1 µL of DpnI (NEB, #R0176L) for 30 min to 3 hrs ...
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bioRxiv - Molecular Biology 2023Quote: RNA was denatured for 5 min at 95°C in 1x RNA Loading Dye (NEB) and separated on denaturing polyacrylamide gels (12% ...
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bioRxiv - Biochemistry 2023Quote: ... while the other was digested at 25 °C with 10 units SwaI (New England Biolabs). Enzymes were inactivated at 65 °C for 20 min ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Samples were cooled to 42 °C and 2 μl of β-agarase I enzyme (NEB) was added and incubated at 42 °C for 90 min with occasional vortexing ...
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bioRxiv - Cell Biology 2023Quote: ... at 37 °C for 1 hour prior to the addition of T4 DNA ligase (NEB). The ligation reaction was incubated at room temperature for 1 hour then transformed into DH5ɑ competent cells.
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bioRxiv - Microbiology 2024Quote: ... 72°C: 1’0 min) with a Q5 high fidelity DNA polymerase (New England Biolabs, M0491) and 10 µM forward gene specific primers also containing an additional UMI sequence (8 random nucleotides ...
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bioRxiv - Cell Biology 2024Quote: ... G or C) and assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). The randomized N12-oligonucleotides were commercially synthesized and purified on a reversed-phase column (FASMAC ...
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bioRxiv - Molecular Biology 2024Quote: ... and fragmentation (25 min at 94 °C in 2X T4 PNK reaction buffer from NEB –140 mM Tris-HCl pH 7.6 ...
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bioRxiv - Molecular Biology 2024Quote: ... Plugs were melted at 70°C and treated with ý-Agarase I (NEB, catalog #M0392) to liberate the DNA ...
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bioRxiv - Biochemistry 2024Quote: Constructs bearing a C-terminal cMyc tag were cloned by site-directed mutagenesis (NEB, M0554S) using oligonucleotides as primers bearing a 5’-overhang encoding the inserted sequence ...
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bioRxiv - Neuroscience 2023Quote: ... C-sbGluc and p2a dTomato by Gibson cloning (New England Biolabs HiFi DNA Master Mix). Coding sequences from plasmids for GCaMP6f ...
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bioRxiv - Genomics 2024Quote: ... pX458 and pX459 vectors were previously digested 2hours at 37°C by BbsI (NEB, R0539) and dephosphorylated 10 minutes at 37°C using Quick CIP (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... Hi-C libraries were treated with T4 DNA Polymerase (New England Biolabs, catalog No. M0203) to remove biotin from unligated ends ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-SNAP (1:1000, polyclonal, New England Biolabs, Ipswich, MA, P9310S), or mouse anti-tubulin (1:40,000 ...
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bioRxiv - Microbiology 2020Quote: ... Anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate, NEB) or anti-mouse IgG (H+L ...
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bioRxiv - Molecular Biology 2022Quote: ... Butyryl-Histone H3 (Lys 9) rabbit pAb (PTM Biolabs, SKU: PTM 305), Butyryl-Histone H3 (Lys 23 ...
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bioRxiv - Molecular Biology 2022Quote: ... Butyryl-Histone H4 (Lys 5) rabbit pAb (PTM Biolabs, SKU: PTM 313), Butyryl-Histone H3 (Lys 27 ...
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bioRxiv - Molecular Biology 2022Quote: ... Butyryl-Histone H3 (Lys 27) rabbit pAb (PTM Biolabs, SKU: PTM 315).
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bioRxiv - Molecular Biology 2022Quote: ... Butyryl-Histone H4 (Lys 12) rabbit pAb (PTM Biolabs, SKU: PTM 308), Butyryl-Histone H3 (Lys 9 ...
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bioRxiv - Molecular Biology 2022Quote: ... Butyryl-Histone H4 (Lys 8) rabbit pAb (PTM Biolabs, SKU: PTM 311), Butyryl-Histone H4 (Lys 5 ...
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bioRxiv - Cell Biology 2020Quote: ... samples were cooled and then digested with DpnII for one hour at 37° C (NEB, R0543). These ligated pools were then amplified using AdR_PCR oligonucleotides as primer (5′-GGTCGCGGCCGAGGATC-3′ ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were thawed at 37°C and mixed with 20 µL of a 500U DNaseI (NEB), 10X buffer and nuclease free water mixture and incubated at 37°C for 30 minutes ...
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bioRxiv - Biophysics 2021Quote: ... 72 °C for 5 minutes) using Phusion High-Fidelity PCR Master Mix with HF buffer (NEB), 0.1 µM of PE1.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... were incubated for 12 hours at 30°C with 7.5 Units of Phi29 polymerase (NEB M0269) in Phi29 NEB buffer 1X ...
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bioRxiv - Systems Biology 2019Quote: ... 30°C and the growth was continuously monitored online using Cell Growth Quantifier (Aquila Biolabs GmBH). Samples were withdrawn at regular intervals for analyzing the spent metabolites through High-Performance Liquid Chromatography (HPLC).
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bioRxiv - Microbiology 2019Quote: ... which was placed towards the centre of a C760 Innova – 70 °C freezer (New England Biolabs) for 1 h before being thawed in a 37 °C waterbath for approximately two min.
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bioRxiv - Molecular Biology 2020Quote: ... After incubation for min at 55°C 1 μl T4 ligase (400,000 units/ml, NEB, M0202) was added ...