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Citations for New England Biolabs :
251 - 300 of 1156 citations for c Myc Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... a CP29A specific rabbit antisera (Kupsch et al., 2012) or anti-SNAP rabbit antisear (NEB, Frankfurt, Germany) in 1:2000 dilution overnight at 4°C and subsequently washed with TBST buffer four times at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Final concentrations of 5 µM TDP-43-TEV-MBP and the indicated final concentrations of recombinant proSAAS or BSA (NEB BioLabs; B9001S) were achieved by mixing aliquots of a 44 μM stock solution of TDP-43-TEV-MBP with aliquots of stock solutions of either 55 μM proSAAS or 151 μM BSA (both in 5 mM acetic acid) ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplified PCR products were ligated into the recombinant plasmid pZE0-P-MglE-T using the recommended standard USER cloning protocol (NEB, UK).
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bioRxiv - Developmental Biology 2021Quote: ... After annealing the complex an equimolar amount was mixed with 1000 ng Cas9 recombinant protein (NEB; final concentration 20 ng/μL) and incubated at RT for 15 min ...
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bioRxiv - Cell Biology 2022Quote: Phase separation of purified recombinant Cdc15-IDR-SH3 co-expressed with Pom1 was induced by treatment with α-phosphatase (NEB; P0753L) and dilution to physiological salt concentrations (50 mM Tris pH 7.4 ...
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bioRxiv - Immunology 2022Quote: The antibody expression vectors for each recombinant antibody (VH + VL-L/k) were transfected together with a Transposase vector (Hera BioLabs, USA). Cells were selected with Hygromycin B (H3274 ...
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bioRxiv - Neuroscience 2022Quote: All anti-tau monoclonal antibodies were generated by the hybridoma approach against recombinant tau aggregates (human full-length tau) encapsulated in the ACM Polymersomes (ACM Biolabs, Singapore) and purified by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant cohesinWT or cohesin3D (25 nM) were incubated with 50 nM NIPBL-MAU2 and 10 ng/μl λ-DNA (NEB, N3011S) in ATP reaction buffer consisting of 20 mM NaH2PO4/Na2HPO4 pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... When needed the mRNA was polyadenylated after transcription using a recombinant poly-A polymerase as recommended by the manufacturer (NEB Biolabs).
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bioRxiv - Molecular Biology 2023Quote: ... Unligated 3’ linker was removed by incubating the samples with the 5’-deadenylase KmHnt3 (see Recombinant protein expression and purification) and RecJ exonuclease (New England Biolabs, M0264S) for 45 min at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... All recombinant plasmids in this study were constructed with NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs, Ipswich, Massachusetts, USA). All oligonucleotides were ordered and all sequencing works were done at Eurofins Genomics (Ebersberg ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal anti-lactyllysine antibody (PTM Biolabs), or mouse monoclonal anti-β-actin antibody (A5316 ...
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bioRxiv - Systems Biology 2022Quote: ... anti-SNAP (1:1000, rabbit, NEB, P9310S); anti-MRPS18B ...
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bioRxiv - Synthetic Biology 2023Quote: ... rabbit anti-Sox-2 (New England Biolabs), and rabbit anti-NANOG (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and rabbit anti-NANOG (New England Biolabs). Briefly ...
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bioRxiv - Genetics 2023Quote: ... and anti-rabbit HRP conjugated (NEB #7074) and anti-mouse HRP conjugated (Cell Signaling Technologies #7076 ...
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bioRxiv - Genetics 2021Quote: ... and assembly of the recombinant vector was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA, USA). To generate the double ΔnoxA Δyap1 knockout mutant ...
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bioRxiv - Neuroscience 2022Quote: ... Final concentrations of 5 µM TDP-43-TEV-MBP and the indicated final concentrations of recombinant proSAAS or BSA (NEB BioLabs; B9001S) were achieved by mixing aliquots of a 44 μM stock solution of TDP-43-TEV-MBP with aliquots of stock solutions of either 55 μM proSAAS or 151 μM BSA (both in 5 mM acetic acid) ...
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bioRxiv - Molecular Biology 2022Quote: ... Additional reconstitutions with linker histone were performed by mixing reconstituted 183×12 core arrays with 1 mg/ml recombinant linker histone variant H10 (New England Biolabs, cat.# M2501S) at a molar ratio of 0.7 molecule histone H1 per nucleosome in solution containing 500 mM NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... Histone yields from each purification were determined by using a calibration curve of recombinant H3 and H4 (NEB, cat. #M2503S, #M2504S, respectively). Absolute amounts of histone were determined by densitometry on coomassie-stained bands with Fiji61 for Pristionchus data (conducted at the MPI in Tübingen ...
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bioRxiv - Genomics 2019Quote: ... and SalI-HF (37 °C, 90 min, New England Biolabs). The fragments were column-purified ...
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bioRxiv - Developmental Biology 2022Quote: ... overnight at 37°C and NEBuilder HiFi Assembly MasterMix (NEB) was used for the assembly of the fragments ...
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bioRxiv - Pathology 2020Quote: ... at 65⁰C for 2h followed by MspI (NEB R0106) at 37⁰C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... dmGemin2 was fused to a C-terminal Mxe intein (NEB) containing a hexahistidine tag ...
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bioRxiv - Biochemistry 2019Quote: ... 37°C with vaccinia virus capping enzyme (New England Biolabs), as per the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... 1 µL of 0.4 µg µL-1 Lys-C (NEB) stock was added (enzyme:substrate ratio of 1:100 w/w ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C) and filled in using T4 DNA polymerase (NEB) using manufacturers’ instructions at 12°C for 20 min75 ...
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bioRxiv - Plant Biology 2024Quote: ... and ligated overnight at 16°C (T4 DNA ligase; NEB). The same approach was used for the second deletion (353 bp ...
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bioRxiv - Bioengineering 2023Quote: ... and at 37 °C for MscI (New England Biolabs, R0534) and BstEII-HF (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin was digested with Microccocal Nuclease (NEB, 37°C, 30 min) in digestion buffer (1M Tris-HC ...
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bioRxiv - Molecular Biology 2021Quote: ... and 72°C for 30 sec) with Q5 polymerase (NEB Inc.) and appropriate primers (S2 Table).
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bioRxiv - Developmental Biology 2020Quote: ... for 30□min in 37°C and proteinase K (NEB, P8102S) for 2□h in 37°C and de-crosslinked in 65□°C for 8□h ...
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bioRxiv - Developmental Biology 2022Quote: ... 72°C for 1 min)) using NEBNext 2x Master Mix (NEB) and Nextera adapters and put on ice ...
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bioRxiv - Microbiology 2019Quote: ... were digested for 1.5 hrs at 37°C with NotI (NEB) to introduce the bla gene at the unique NotI site of pPEPZ-Plac (spec) ...
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bioRxiv - Biochemistry 2020Quote: ... samples were digested overnight at 37°C with PNGase F (NEB). After the N-linked glycans were removed ...
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bioRxiv - Plant Biology 2021Quote: ... Digestions were performed overnight at 37°C with 400U DpnII (NEB). DNA was ligated by incubation at 16°C for 5h in 4 ml volume using 100U of T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C overnight and then 100ng GluC (New England BioLabs) was added for another overnight incubation at 25°C ...
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bioRxiv - Biophysics 2019Quote: ... for 15 minutes at 12°C in 1x buffer 2.1 (NEB), to remove 3’-A overhangs.
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bioRxiv - Molecular Biology 2021Quote: ... 7 units USER Enzyme for 30 minutes at 37°C (NEB)51 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Mixes were subsequently digested 1h at 37 °C with DpnI (NEB) and used for electroporation in E ...
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bioRxiv - Genetics 2019Quote: ... for 1 hr at 37°C followed by Exonuclease T (NEB) for 45 min at 24°C to blunt DNA ends before Illumina adapter ligation (Canela et al. ...
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bioRxiv - Plant Biology 2023Quote: ... Digestions were performed overnight at 37°C with 400U DpnII (NEB). DNA was ligated by incubation in a shaker at 16°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 72°C for 1 min) using NEBNext 2x Master Mix (NEB) and Nextera adapters and put on ice ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour at 37°C in 1x ThermoPol buffer (NEB). Samples were processed and analyzed as described15.
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bioRxiv - Molecular Biology 2024Quote: ... and 72°C for 30 sec) with Q5 polymerase (NEB Inc.) and appropriate primers (Supp ...
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bioRxiv - Microbiology 2020Quote: ... rabbit anti-SNAP-tag (P9310, New England BioLabs), rabbit anti-beta-tubulin (NB600-936 ...
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bioRxiv - Cell Biology 2020Quote: ... Pan anti-trimethyllysine (rabbit, PTM Biolabs, PTM-601), SETD2 (rabbit ...
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bioRxiv - Cell Biology 2019Quote: ... or rabbit anti-GAPDH (2118S NEB, 1:5000) primary antibody with horseradish peroxidase-conjugated anti-mouse or anti-rabbit (Dianova 1:10,000 ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-Gaussia luciferase (E8023, New England Biolabs) at a 1:2,000 dilution ...
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bioRxiv - Synthetic Biology 2021Quote: ... for 4 hours at 37°C and treated with Antarctic phosphatase (NEB) for 30 min at 37°C ...