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Citations for New England Biolabs :
401 - 450 of 2182 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: In vitro transcription reactions were performed according to standard protocols provided with the high-yield in vitro transcription kits (E2040 and E2070, New England Biolabs), consisting of 40 mM rNTP (pH buffered with sodium phosphate ...
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bioRxiv - Zoology 2022Quote: ... The T7 reverse-complement sequence was annealed to the probe sets to allow transcription into RNA and biotinylation in a single reaction using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) followed by a Dnase treatment to avoid sample contamination by probe DNA during the capture ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 μg of total RNA was used for cDNA synthesis by reverse transcription reaction with Protoscript II reverse transcriptase (New England Biolabs). Quantitative PCR was performed using SensiFAST™ SYBR kit (BIOLINE) ...
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bioRxiv - Molecular Biology 2022Quote: ... The yrbA-fs15 DNA templates (with TAG or TGA stop codons) used for in vitro transcription/translation reactions in the classic PURExpress system (New England Biolabs) were prepared by PCR ...
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bioRxiv - Microbiology 2023Quote: ... and p.2.5’ genomic RNA was generated by in vitro transcription using the HiScribe™T7 ARCA mRNA kit (New England Biolabs). For ZIKV-WT ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed on 500 ng of total RNA using a LunaScript™ RT SuperMix Kit (New England Biolabs) as according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed on RNA samples using the ProtoScript II first strand cDNA synthesis kit (New England BioLabs; E6560S) with HRV serotype-specific reverse primers (Rev 5’- -3’) ...
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bioRxiv - Microbiology 2023Quote: ... was used as a template for in vitro transcription using Hi-T7 RNA Polymerase and Ribonucleotide Solution Mix (both from New England Biolabs) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... When needed the mRNA was polyadenylated after transcription using a recombinant poly-A polymerase as recommended by the manufacturer (NEB Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... and primers listed in (Extended Data Table 3) and used for in vitro transcription by T7 RNA polymerase (New England Biolabs). Resulting RNA was purified using a spin-column kit (RNeasy mini kit ...
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bioRxiv - Biochemistry 2023Quote: ... was prepared by in vitro transcription using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs Inc., Massachusetts, USA) with 1 unit RNase Inhibitor (New England Biolabs Inc. ...
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bioRxiv - Developmental Biology 2023Quote: ... MYC) were made in-house by in vitro transcription using mRNA synthesis with HiScribeTM T7 ARCA mRNA Kit (NEB E2060S) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... was obtained by reverse transcription (RT) using 1 μg of RNA and Moloney murine leukaemia virus (M-MuLV) reverse transcriptase (M0253, NEB), using the first strand cDNA synthesis standard protocol with random primers (S1330 ...
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bioRxiv - Biochemistry 2023Quote: ... sgRNA of NmeCas9 was synthesized by in vitro transcription with T7 RNA polymerase and plasmid DNA templates linearized by HindIII-HF (New England Biolabs). The sgRNA sequence was listed in Supplementary Table S1 ...
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bioRxiv - Biochemistry 2022Quote: Drosocin-ribosome complexes were generated by in vitro transcription-translation reactions in PURExpress ΔRF123 in vitro protein synthesis system (New England Biolabs) with the same reaction mix as described earlier in the toeprinting assays ...
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bioRxiv - Genetics 2023Quote: ... we first amplified the 4,165 bp region upstream of the transcription start site of the betta actb gene using Q5 polymerase (M0491S, New England Biolabs) from an ornamental betta ...
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bioRxiv - Biochemistry 2023Quote: ... A 200nt polyA tail was added through PCR and in vitro transcription was carried out using the HiScribe T7 High Yield RNA Synthesis kit (NEB). An m7G cap was introduced by using Vaccinia capping enzyme (NEB) ...
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bioRxiv - Synthetic Biology 2023Quote: ... A plasmid harboring Cas9 and an empty guide RNA transcription cassette was linearized by NotI/BsaI double digestion (New England Biolabs) and transformed into yeast together with a linear piece of DNA containing the guide RNA sequence flanked on either side by homology to the plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: ... were combined into a transcription unit plasmid in a Type IIS DNA assembly reaction using BsaI-HFv2 (New England Biolabs). The destination vector (p15A origin of replication ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was obtained by reverse transcription of 0.5 μg of total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB, #E6560S). Three independent biological replicates were prepared for each genotype ...
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bioRxiv - Microbiology 2023Quote: ... The amount of RNA template was equalized for reverse transcription using the Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs) and random hexamers ...
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bioRxiv - Plant Biology 2023Quote: circRNAs were produced by in-vitro transcription from annealed DNA oligonucleotide templates (Table S2) using HighScribe T7 high-yield RNA synthesis kit (NEB) along with ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... 500ng of total RNA was used for reverse transcription using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs). For quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... Short nucleotide sequences were used for transcription according to the HiScribe Quick T7 High Yield RNA Synthesis Kit (NEB, UK) protocol ...
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bioRxiv - Genetics 2023Quote: ... The presence of infectious rIBV in the allantoic fluid was confirmed by a two-step reverse transcription polymerase chain reaction (RT-PCR) protocol using Protoscript II reverse transcriptase (NEB) and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription and quantitative real-time PCR was performed using Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in a 96-well plate and qPCR was performed in a LightCycler 96 System (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA template used for in vitro transcription was generated by PCR amplification from the corresponding reporter plasmid using the Q5 High-Fidelity DNA Polymerase (NEB) with a reaction including a forward primer containing the T7 promoter sequence and a 60T reverse primer for polyadenylation ...
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bioRxiv - Microbiology 2024Quote: ... according to manufacturer’s instructions using 100 nM RNA-specific reverse transcription primer followed by RNase H digest with 5 µL RNase H (NEB) at 37 °C for 20 min ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR reaction was then used as a template for in vitro transcription using EnGen® sgRNA Synthesis Kit (NEB), and the MEGACLEAR Transcription Clean-Up KIT (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The unpurified DNA product was then subjected to in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), incubating at 37 °C for 16 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The 72mer Ψ-containing model RNA used for mutation analysis and the 1.8-kb 10% Ψ-modified RNA used for UHPLC-MS/MS were prepared by T7 in vitro transcription using HiScribe T7 High Yield RNA Synthesis Kit (NEB) and Pseudo-UTP (Jena Bioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... T7 promoter region was added to the 5’ end of each construct for in vitro transcription of RNA using T7 RNA polymerase from T7 HiScribe RNA synthesis kit (New England Biolabs). Synthesized RNA was subjected to DNase treatment (TURBODNase ...
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bioRxiv - Molecular Biology 2024Quote: SINV was produced from pT7-SVwt plasmid90 that was first linearized with XhoI and purified to use it as a template for in vitro RNA transcription with HiScribe T7 ARCA mRNA kit (NEB). Transcribed viral RNA was transfected into BHK-21 using Lipofectamine 3000 reagent (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... coli constitutive ribosomal promoter J23119 with a poly-T region directly downstream of the sgRNA sequence for transcription termination using KLD mutagenesis (New England Biolabs). pBR322 and pET28 expression plasmids were co-transformed into E ...
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bioRxiv - Cancer Biology 2024Quote: ... Double-stranded cDNA was then synthesized by reverse transcription and underwent end-repair and ligation using New England Biolabs (NEB) adapters ...
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bioRxiv - Biochemistry 2024Quote: ... The HDV sequence was cloned into the XbaI/EcoRV sites of the AVA421 vector to enable linearization for run-off transcription by digestion with EcoRV-HF (NEB) in a reaction containing 1X CutSmart buffer and 0.4 U/μl EcoRV-HF.
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bioRxiv - Cancer Biology 2024Quote: ... PCR-amplified DNA templates encoding 5’UTR of ATF4 mRNA were used for in vitro transcription of ATF4 reporter mRNA using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Biophysics 2023Quote: ... After 4 hours of incubation (required for transcription) RNA was directly treated with 4 units of DNase I (NEB, M0303S) to remove linear or circular DNA used as a template for transcription (see details in Supplementary Methods) ...
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bioRxiv - Developmental Biology 2024Quote: ... Complementary DNA (cDNA) was prepared from total RNA (5 μg) by reverse transcription using LunaScript® RT SuperMix Kit (NEB). qPCR reactions were performed using Power SYBR Green Master Mix (Thermo ...
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bioRxiv - Cancer Biology 2022Quote: ... antibody and anti-Gluc antibody (New England Biolabs, ref. E8023).
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bioRxiv - Developmental Biology 2023Quote: ... a MBP antibody (Anti-MBP Monoclonal Antibody, HRP conjugated, NEB), a GST antibody (GST Tag Monoclonal Antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... in a 10 μl reaction using 10 U T4 RNA ligase (New England Biolabs) in 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Genomics 2023Quote: ... 25 µl of 10×CutSmart Buffer and 10 µl of Hae III (NEB, #R0108L) were added to each tube ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μL RNAs were mixed thoroughly with 1 μL T4 PNK (10 U, NEB), 1 μL purified TS2126 and 1 μL PAP1 enzyme (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... coli (10-beta, NEB, C3020K) were thawed on ice and mixed with 6 µg of purified 3Cs-DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 U of HaeIII (NEB), and >0.1 pg of genomic template DNA ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli NEB 10-beta (NEB) was used for cloning ...
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bioRxiv - Genomics 2021Quote: ... 10 μL buffer 3.1 (NEB) and ultrapure water to a final volume of 70 μL ...
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bioRxiv - Genetics 2021Quote: ... 10 μl DpnII (R0543M, NEB) (500 U per tube ...
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bioRxiv - Bioengineering 2020Quote: ... 10 units BsaI (NEB #R3733), 10 units PNK (Thermo Fisher) ...