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Citations for New England Biolabs :
451 - 500 of 2182 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 10 units BsaI-HFv2 (NEB), 100 units T4 DNA ligase (NEB) ...
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bioRxiv - Genetics 2020Quote: ... 10 μl MboI (NEB R0147S), 5 μl CviQI (NEB R0639S) ...
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bioRxiv - Microbiology 2022Quote: ... 10 mm NTP mix (NEB), Ribolock (Thermo scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells (NEB 10-beta). The recombinant N-terminal His6-tagged TrxA protein encoded by slr0623 was expressed and purified as previously described previously for His-tagged proteins (Lapina et al ...
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bioRxiv - Synthetic Biology 2022Quote: ... 10 U BsaI-HFv2 (NEB) for assembly into levels 1 and 3 or 10 U BpiI (Thermo Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 10 µg λ DNA (NEB) was added to a 50 µL reaction with 80 µM biotin-dCTP (Invitrogen) ...
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bioRxiv - Plant Biology 2019Quote: ... 10 μl PNK enzymes (NEB) and 15 μl of 10mM ATP and incubated at 37°C for 10 mins on a Thermomixture (1400 rpm) ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10-beta (NEB C3020) cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 U MseI (NEB, R0525S), 1x ddPCR supermix for probes and 50-300 ng of genomic DNA (gDNA) ...
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bioRxiv - Biochemistry 2021Quote: ... E.coli (NEB® 10-beta) containing both a Cas effector and gRNA plasmid (Table S3 ...
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bioRxiv - Biochemistry 2021Quote: ... 10 U T5 exonuclease (NEB) were added ...
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bioRxiv - Cell Biology 2020Quote: ... with 10 mM VRC (NEB) per coverslip for 10 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... 0.23μl 10 mM dNTPs (NEB), 0.08 μl 10 U/μl E ...
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bioRxiv - Plant Biology 2022Quote: ... 10 units of BsaI (NEB), and 200 units of T4 DNA ligase (NEB) ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μL PNGase F (NEB) and 10 μL water were added into the reaction system ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 nL PEG8000 (50%, NEB), 1 nL BSA (20 ng/ml NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 U Terminal Transferase (NEB) and ddH2O to a volume of 50 µl ...
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bioRxiv - Bioengineering 2023Quote: ... 10-beta cells (NEB #C3020K) or TOP10 (Invitrogen #C404010 ...
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bioRxiv - Cell Biology 2023Quote: ... 10 mM ATP (NEB, P0756), 10x rCutSmart Buffer ...
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bioRxiv - Microbiology 2023Quote: ... and 10 nmol ATP (NEB) were added to the annealed products ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ul rCutSmart Buffer (NEB). The mixes of retrons were organized according to their relative production to retron-Eco1 ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μl RNase H (NEB), and 70 μl nuclease-free H2O to the cDNA mixture and incubation at 37 °C for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 units PNK (NEB) in 50 μl PNK buffer 1X (30’ at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of in vitro transcribed 300 nM sgRNA or trL (produced as described in in vitro transcription protocol above) and 1 μL of 1 μM Cas9 nuclease (NEB, M0386S) were mixed and incubated for 10 minutes at 25°C to allow for RNP formation ...
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bioRxiv - Cell Biology 2020Quote: ... the pT7-SVmCherry plasmid was first linearised with XhoI and used as a template for in vitro RNA transcription with HiScribe T7 ARCA mRNA kit (New England Biolabs, #E2065S). Transcribed genomic RNA was transfected into BHK-21 using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: Reverse transcription was performed by adding the following to the above reaction: 8 uL of 5x first strand buffer (NEB E7330L), 2 uL of 10mM dNTPs (each) ...
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bioRxiv - Developmental Biology 2021Quote: ... A T7 RNA polymerase binding site with short 5’-tail (aaaaTAATACGACTCACTATAG) was added to reverse primers for transcription using T7 RNA polymerase incorporating DIG labelled ribonucleotides (NEB, Roche). PCR amplified probe templates were confirmed by sanger sequencing.
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bioRxiv - Genomics 2020Quote: ... Purified dsDNA templates were used as templates for T7 transcription reactions using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) and bio-16-CTP (Trilink ...
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bioRxiv - Molecular Biology 2021Quote: ... were used as template of the in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, #E2050S, New England Biolabs), performed at 37°C for 16 hr ...
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bioRxiv - Biochemistry 2020Quote: ... and 8 pmol of purified DNA was then used for in vitro transcription with T7 RNA polymerase (New England Biolabs Inc.). The resulting RNA was purified with Agencourt RNAClean XP beads supplemented with an additional 12% of PEG-8000 (3 volumes of 40% PEG-8000 was added to 7 volumes Agencourt RNAClean XP beads ...
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bioRxiv - Genomics 2019Quote: ... The annealed oligos were used as the templates for in vitro transcription (IVT) using the HiScribe™ T7 High Yield RNA Synthesis Kit (Cat. E2040S, NEB). After IVT ...
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bioRxiv - Biochemistry 2019Quote: RNA was produced by in vitro transcription (IVT) using the HiScribe T7 High Yield RNA Synthesis Kit (New England BioLabs, E2040S). DNA templates for the IVT reaction were produced by annealing complementary DNA oligos (IDT ...
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bioRxiv - Bioengineering 2019Quote: ... Unlabeled tracrRNAs were synthesized by IDT or through in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, NEB) using DNA templates containing a T7 promoter binding sequence and full length tracrRNAs ...
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bioRxiv - Biochemistry 2020Quote: ... molecules used in this study were synthesized by in vitro transcription using HiScribe™ T7 Quick High Yield RNA Synthesis Kits (New England Biolabs), or transcribed directly in the in vitro translation (IVT ...
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bioRxiv - Microbiology 2020Quote: ... and in vitro bacterial cultures was used as template in reverse transcription reactions using the Murine Moloney Leukemia Virus Reverse Transcriptase (NEB M0253S) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... The pool of partially double stranded DNA templates was used for in vitro transcription with HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England BioLabs) and the RNA was purified with TRizol (Ambion) ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were chosen to minimize predicted off-target effects with maximum cleavage efficiency and were synthesized by in vitro transcription as previously described26 using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040S) or ...
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bioRxiv - Cell Biology 2021Quote: ... the previous PCR products was used as template to amplify and convert into RNA via an in vitro transcription of high yield (New England Biolabs, E2040S); the RNA product above were converted back into single-stranded DNA via reverse transcription ...
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bioRxiv - Molecular Biology 2022Quote: The rest of RNAs were performed with real-time reverse transcription PCR (RT-qPCR) using a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs) according to the manufacturer’s recommendations by a thermocycler (BIO-RAD CFX96™ Real-Time System) ...
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bioRxiv - Cancer Biology 2020Quote: ... 25uM RibOxi RT primer was then annealed to the reaction before ligation of 5’ RNA linker using T4 RNA ligase I at room temperature for 1 hour and subsequent reverse-transcription using Protoscript II (NE Biolabs, M0368S). cDNA was then purified with AmpureXP beads with 1:1.8 ratio ...
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bioRxiv - Microbiology 2021Quote: ... 10 ul of this was then used as an input for T7 polymerase mediated In vitro Transcription (IVT) using the NEB HiScribe T7 High Yield RNA Synthesis Kit (NEB, # E2040S). Briefly ...
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bioRxiv - Immunology 2019Quote: ... T7 promoter sequences were added by PCR and the PCR product was used as template to generate sgRNAs by in vitro transcription using the HiScribe T7 quick high yield RNA synthesis kit (NEB E2050S) and purified by an RNeasy mini kit (Qiagen 74104) ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid was linearized with BamHI digestion and further recovered as a template for in vitro transcription via HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). RNA was isolated by 15% denaturing PAGE and dissolved in stocking buffer (300 mM NaAc pH 5.2 ...
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bioRxiv - Biochemistry 2020Quote: GQES7-a and GQES7-b were synthesized in vitro by transcription (HiScribe™ T7 High Yield RNA Synthesis Kit; New England Biolabs). GQes3 and mutes3 ...
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bioRxiv - Biophysics 2020Quote: ... and the corresponding RNAs were synthesized via in vitro transcription using the HiScribeTM T7 Quick High Yield RNA Synthesis Kit (New England Biolabs Inc.) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... 200-500 ng of the purified PCR product was used as DNA template in 25 μl of coupled in vitro transcription and translation reaction using PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L). The reaction was incubated at 37°C for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... We then performed in vitro transcription from these amplicons using the HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs), and purified the resulting RNA using a GeneJET RNA Purification Kit (Thermo Scientific) ...
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bioRxiv - Genomics 2021Quote: ... were used as template of the in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit; #E2050S, New England Biolabs), performed at 37°C for 16h ...
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bioRxiv - Genetics 2020Quote: ... In vitro transcription template was amplified with T7 promoter sequence containing primer and in vitro transcribed using T7 RNA polymerase (NEB, E2040S). The IVT RNAs were capped using Vaccinia virus capping enzyme (Cellscript ...
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were used as templates for in vitro transcription using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, New England Biolabs). Cas9 mRNA and gRNAs were then purified using RNAClean XP(Beckman Coulter ...