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Citations for New England Biolabs :
401 - 450 of 3850 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... coli (NEB, C2566) and transformed cells were plated on selective Kan LB agar plates and incubated overnight at 30 °C.
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bioRxiv - Immunology 2024Quote: ... coli (NEB, #C3040H) at 30 °C in LB medium ...
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bioRxiv - Microbiology 2024Quote: ... coli C2925 (NEB). Cloned gene expression was induced by inclusion of the indicated concentration of L-tryptophan (Trp ...
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bioRxiv - Biochemistry 2024Quote: ... coli PPIase (NEB), 5 U µL-1 T7 RNA polymerase (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... coli cells (NEB) were transformed with plasmids encoding β-gal RNC constructs and grown in ZYM-5052 autoinduction media 106 for 18 h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Coli (NEB, C2527I) were grown on LB (Luria-Bertani ...
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bioRxiv - Cell Biology 2024Quote: ... coli cells (NEB) and identified by colony PCR followed by Sanger sequencing.
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bioRxiv - Biochemistry 2024Quote: ... coli (NEB, C2527H) via autoinduction ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli DH5α (NEB). Escherichia coli strains and plasmids used are listed in Supporting Information ...
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bioRxiv - Bioengineering 2024Quote: ... coli T7SHuffle (NEB). pET28a and pETDuet constructs were transformed into chemically competent E ...
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bioRxiv - Physiology 2023Quote: ... coli (C2987H, NEB) by heat shock at exactly 42°C for exactly 30 seconds ...
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bioRxiv - Developmental Biology 2019Quote: ... A short stretch of genomic region (∼600-800 bp) flanking the target site was amplified using Q5 Hot Start High Fidelity 2 × Master Mix (NEB). Amplified sequences were purified with the QIAquick PCR purification kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: The miRCat-33 3’ linker was ligated to the 3’ end of the RNAs on the Ni-NTA beads with 800 units of T4 RNA ligase 2 truncated K227Q (New England Biolabs) in 1 x PNK buffer / 16.67% PEG 8000 in the presence of 80 units RNasin in a total volume of 80 µl ...
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bioRxiv - Biophysics 2022Quote: ... 2 μL (1000 U) PNGase F (New England Biolabs Inc) were added and the reaction was incubated for 6 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: Single mutations of the spike protein were generated via two PCR fragments of the spike ORF using high-fidelity Phusion polymerase (New England Biolabs, USA). The first fragment was generated via a generic forward primer (pCAGGS-5 ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 S R403T and RaTG13 S T403R/T403A mutant plasmids were generated using Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Biochemistry 2021Quote: Native glycoproteins (10 μg) were digested with 1 U of IMPa (O-Glycoprotease, New England Biolabs) in 50 μL 20 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2 mM CaCl2 and the TRX-6His-S-tag was cleaved overnight at RT with enterokinase protease (NEB). The ET domain was further purified using Nickel-NTA resin (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The SNAP-tag sequence (NEB) was cloned into a β-cell-specific expression vector with a porcine INS gene promoter ...
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bioRxiv - Synthetic Biology 2022Quote: ... rabbit anti-SNAP-tag (NEB; P9310 ...
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bioRxiv - Immunology 2022Quote: ... Delta and BA.2 spike plasmids were linearized by restriction enzymes and transcribed to mRNA by in vitro T7 RNA polymerase (NEB, Cat # E2060S) as previously described10.
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bioRxiv - Cell Biology 2021Quote: ... m6A-modified RNA spike-in controls (New England Biolabs) and a nontargeted region on the crRNA-targeted transcript ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of 10 mM MnCl2 and 1-2 μl (400-800 units) of λ PP (New England Biolabs, Ipswich, MA). Untreated lysates received 1-2 μl of water in place of λ PP ...
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bioRxiv - Molecular Biology 2020Quote: ... 800 U T4 DNA Ligase (New England BioLabs), and ultrapure water ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 4 μl 50% PEG-800 (New England Biolabs), 4 μl 10× T4 RNA ligase buffer (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... then ligated with 800 U T4 ligase (NEB) for 4 hours at 16°C followed by 30min at RT ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and 800 U T4 DNA ligase (NEB, M0202S). Viral LTR–host DNA junctions were amplified using semi-nested PCR with a unique linker-specific primer and LTR primers ...
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bioRxiv - Cell Biology 2022Quote: Lysates (20 μg) and media immunoprecipitates were diluted to 10 μL in 2X glycoprotein denaturing buffer (NEB), and boiled for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... N-linked glycans released from glycoproteins using Peptide:N-glycosidase F (PNGase F, New England Biolabs, Ipswich, MA) were mixed with 2,5-dihydroxybenzoic acid (DHB ...
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bioRxiv - Bioengineering 2020Quote: ... coli DH10B strain (NEB #C3019 New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... coli (New England BioLabs) to produce demethylated plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli DH5α cells (NEB).
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bioRxiv - Cell Biology 2020Quote: ... coli DH5α cells (NEB). The ‘601’ fragments were released from the plasmid by digestion with EcoRV (NEB) ...
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bioRxiv - Genetics 2021Quote: ... coli cells (NEB, C2987H) and plated on LB/ampicillin/IPTG/X-gal plates (according to pGEM-T Easy Vector System manufacturer’s instructions) ...
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bioRxiv - Microbiology 2019Quote: ... coli 70S ribosome (NEB). Lead acetate mediated cleavage was performed as described above ...
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bioRxiv - Biochemistry 2022Quote: ... coli NEBα (NEB, Canada) competent cells and were purified using NEB Monarch Miniprep Kits (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... coli DNA ligase (NEB) and DNA polymerase I (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli DNA ligase (NEB), and DNA Pol I (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... coli Topoisomerase I (NEB).
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bioRxiv - Molecular Biology 2020Quote: ... coli Ribonuclease H (NEB) or ddH2O in 1xRibonuclease H buffer (2 h ...
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bioRxiv - Cell Biology 2022Quote: ... coli cells (NEB # C2987) were transformed with the ligation product and plated following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... coli (New England BioLabs). E ...
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bioRxiv - Cell Biology 2019Quote: ... coli inorganic pyrophosphatase (NEB), 4 U/μl T7 RNA polymerase (NEB - pH 7.9 ...
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bioRxiv - Biochemistry 2019Quote: ... coli (New England Biolabs) with 1mM IPTG induction in a shake flask with 1L LB supplemented with 10mM Glucose ...
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bioRxiv - Microbiology 2019Quote: ... coli (New England Biolabs) by heat shock ...
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bioRxiv - Bioengineering 2019Quote: ... coli BL21 (DE3) (NEB) leading to the PcaV and Van2 proteins ...
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bioRxiv - Bioengineering 2019Quote: ... coli DH5-alpha (NEB), transformation for induction test was made into E ...
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bioRxiv - Molecular Biology 2020Quote: ... coli (New England Biolabs). Cells were grown at 37°C in Lysogeny Broth (LB ...
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bioRxiv - Microbiology 2019Quote: ... coli NEB5α cells (NEB).Sequence-confirmed clones were co-transformed (pUT18 and pKNT25 ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... coli (New England BioLabs), and 1% of the transformation reaction was plated to estimate library coverage by counting colonies ...