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Citations for New England Biolabs :
401 - 450 of 4239 citations for Recombinant Human FCGRT & B2M Protein His Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... recombinant HAΔTM H1N1 A/Michigan/45/2015 and H3ΔTM H3N2 A/Hong Kong/4801/2014 were biotinylated and coupled to streptavidin magnetic beads (New England Biolabs) at a ratio of 2.5 μg of protein to 25 μl of beads ...
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bioRxiv - Microbiology 2019Quote: In vitro transcribed 3X-FLAG tagged manY transcripts – wild-type or Δenh (1 pmol) were translated in vitro using the PURExpress translation kit (NEB). Translation reactions were stopped by adding Laemmli sample buffer (Bio-Rad) ...
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bioRxiv - Biochemistry 2019Quote: ... Quantification of the ratio of MS2-tagged rRNA to WT rRNA was done via semi-quantitative PCR with Q5 DNA polymerase (NEB). Primers MS2_quant_F/R bind outside the MS2 tagged region of 23S cDNA ...
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bioRxiv - Microbiology 2021Quote: The coding sequence of CTSL was tagged with a 3’V5 and cloned into CSIN using NEBuilder HiFi DNA Assembly (NEB) with primers CTSL_3’_V5_F ...
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bioRxiv - Plant Biology 2021Quote: Blunt ended Myc tagged GUX and AtMAGT1 CDSs were amplified from a synthetic construct (synthesised by Genewiz) with Q5 DNA Polymerase (NEB) using primers detailed in Table S1 ...
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bioRxiv - Plant Biology 2021Quote: ... SPL8 and SPL15 were amplified from cDNA to generate the respective tagged constructs using Q5 high fidelity DNA polymerase (NEB). The primers (listed in Table 5 ...
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bioRxiv - Microbiology 2021Quote: ... harboring the p6- GLuc / p6 genomes or pTM-ORF1 vectors and epitope-tagged inserts were restricted by the suitable restriction enzymes (Table 1) and subsequently ligated using the T4 DNA ligase (NEB). Finally ...
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bioRxiv - Genetics 2020Quote: ... and T3 (5’-AATTAACCCTCACTAAAGGG-3’) promoter-tagged PCR fragment from each gene using corresponding T7 and T3 RNA polymerase (T3:M0378S; T7:M0251S, BioLabs). Primers used for PCR are listed in Supplemental table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids and siRNAs Bacterial expression constructs for His6-tagged p97 fragments were generated by Q5 Site-Directed mutagenesis (New England Biolabs) of pET15b-His6-p97 plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... the SNAP-tagged fragment was assembled into a ApaI digested MBO2-HA plasmid using NEBuilder (New England Biolabs, Ipswich, MA). The final N-SNAP-MBO2-HA construct encodes an MBO2 polypeptide with a SNAP tag at its N-terminus and a 2-HA tag located between amino acids 885 and 886 of the original MBO2 sequence (Supplemental Figure S1).
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bioRxiv - Cell Biology 2023Quote: ... To remove RNA secondary structure and anneal the mRNA capture primer 1 μL of tagged random hexamer (100 μM) and 0.5 μL of 10 mM dNTPs (dNTP solution set NEB - N0446S) were added to the sample ...
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bioRxiv - Microbiology 2023Quote: ... brucei BFs CN PIP5Pase exclusively expressing V5-tagged PIP5Pase WT or mutant D360A/N362A for 24 h using the magnetic mRNA isolation kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and the GeneArt Gibson Assembly HiFi Master Mix (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3xFLAG- and HA-tagged ZNRF3 expression vectors were generated with the Q5 Hot Start High-Fidelity DNA Polymerase Kit (New England Biolabs) by replacing the FLAG-tag ...
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bioRxiv - Cell Biology 2020Quote: Libraries were prepared using Next Ultra II kits (NEB) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Phusion hot start II DNA polymerase (New England Biolabs) PCR reaction mix (10µl 5X Phusion HF buffer ...
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bioRxiv - Genomics 2020Quote: ... PCR2 was performed with NEBNext Ultra II Q5 (NEB) using standard Illumina indexed primers (e.g ...
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bioRxiv - Molecular Biology 2021Quote: ... and ProtoScript II First Strand cDNA Synthesis Kit (NEB) with Oligo-dT primers ...
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bioRxiv - Biochemistry 2019Quote: ... cDNA was synthesized using Protoscript II Reverse Transcriptase (NEB) and d(T)20 oligonucleotide according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... ProtoScript-II first-strand cDNA synthesis kit (NEB, USA), Brilliant-III ultrafast SYBR Green qPCR master mix (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... or ProtoScript II Reverse Transcriptase (New England Biolabs (NEB), Beverly ...
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bioRxiv - Cell Biology 2021Quote: ... or ProtoScript II Reverse Transcriptase (New England Biolabs (NEB), Beverly ...
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bioRxiv - Microbiology 2021Quote: ... using the Ultra II FS Kit (New England Biolabs). Libraries were quantified using a BioAnalyzer (Agilent ...
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bioRxiv - Systems Biology 2020Quote: ... the NEBNext Ultra II DNA Library prep kit (NEB) was used for libraries with size ranges from 200-400bp ...
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bioRxiv - Microbiology 2021Quote: ... using the high-fidelity reverse transcriptase Protoscript II (NEB). The reaction was performed in a volume of 20 µl containing 1µg of total RNA ...
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bioRxiv - Microbiology 2020Quote: ... First strand cDNA was synthesised using ProtoScript II (NEB) with 1μg of total RNA as template and 6 μM random primers and incubated for 5 min at 25°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 25 µl NEBNext Ultra II Q5 Master Mix (NEB) and indexed amplification primers (125 nM final concentration ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 U/μl protoscript II reverse transcriptase (NEB, M0368X), 1x protoscript II reverse transcriptase buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... The ProtoScript II First Strand cDNA Synthesis Kit (NEB) was used with 600 ng of total RNA from each sample with the d(T)23 VN primer using the standard protocol in a total volume of 20 µl ...
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bioRxiv - Genomics 2019Quote: ... NEBNext Ultra II DNA (New England Biolabs, MA, USA) was used for DNA library preparation.
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bioRxiv - Molecular Biology 2021Quote: ... 40 U of ProtoScript II Reverse Transcriptase (NEB, #M0368S), 0.5 μM of forward primer ...
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bioRxiv - Molecular Biology 2021Quote: ... The NEBNext Ultra II DNA Library Prep kit (NEB) was used according to manufacturer’s protocol to ligate TruSeq (CD ...
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bioRxiv - Immunology 2021Quote: ... and NEB Ultra II RNA library prep kit (NEB) were used to prepare complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... and reverse-transcribed using ProtoScript II (New England Biolabs). PCR was performed with an equal volume of the acquired cDNA in 25 µl of reaction mixture using PrimeSTAR Max Premix (TaKaRa ...
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bioRxiv - Molecular Biology 2022Quote: ... and Protoscript II reverse transcriptase (New England BioLabs, Inc.), according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 U/μl Protoscript II reverse transcriptase (NEB, M0368X), 1x Protoscript II reverse transcriptase buffer ...
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bioRxiv - Microbiology 2023Quote: ... and NEB Ultra II RNA library prep kit (NEB) were used to prepare complementary DNA (cDNA ...
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bioRxiv - Microbiology 2023Quote: ... the NEBNext Ultra II DNA Library Prep Kit (NEB) was used according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... primarily using PCRs (Q5 ultra II HotStart Polymerase, NEB), Gibson Assemblies (NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were constructed using Ultra II DNA kit (NEB).
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bioRxiv - Genetics 2023Quote: ... using NEBNext Ultra II Q5 master mix (NEB M0544S). The PCR product was purified using Amicon Ultra-2mL 50K centrifugal filter (Millipore UFC205024 ...
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bioRxiv - Immunology 2021Quote: ... The recombinant plasmids (LentiCRISPRv2.gRNA10, LentiCRISPRv2.gRNA11) were then transformed into competent Escherichia coli (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant plasmid was generated by a NEBuilder® HiFi DNA Assembly (New England Biolabs) reaction ...
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bioRxiv - Cell Biology 2020Quote: Recombinant MBP fusions to Nud1 or Nud17A were expressed from pMAL-c4X (New England Biolabs). Induction in Rosetta 2 cells (Novagen ...
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bioRxiv - Cancer Biology 2021Quote: Trastuzumab deglycosylation was achieved by adding 1.1 units of recombinant PNGaseF enzyme (New England BioLabs) per 1 μg of antibody ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant SARS-CoV-2 Omicron RBD was digested with EndoH over night (New England Biolabs). Recombinant hACE2 protein was digested using EndoH (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... To complete the recombinant assembly reaction NEBuilder® HiFi DNA Assembly kit (New England Biolabs) was used with the combination of three different inserts produced by PCR ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction was initiated by addition of 10 U (pmol/min) of recombinant CK1 (NEB), CDK1-cyclin B1 (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of the sgRNA was complexed with 20 pmol recombinant Cas9 endonuclease (NEB # M0646T) and injected into the C ...
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bioRxiv - Genomics 2021Quote: ... The PCR reaction consisted of 25 µL NEBNext Hi-Fidelity 2x PCR Master Mix (New England Biolabs NEB.M0541S), 7.5 µL H2O ...