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Citations for New England Biolabs :
401 - 450 of 672 citations for Mouse Anti Staphylococcus Aureus Enterotoxin G Antibody SEG 16 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 1–10 µg of RNA transcribed in vitro was treated with Escherichia coli poly(A) polymerase (5000 U/mL, New England Biolabs, M0276) at 37°C for 30 min followed by clean-up using an RNeasy Mini kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation at 16,000 × g for 45 min and the supernatant was loaded onto amylose affinity resin (New England BioLabs, Ipswich, MA). The column was washed with 10 CVs of lysis buffer and the protein was eluted with sequentially added 1 CV aliquots of lysis buffer Supplemented with 20 mM maltose ...
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bioRxiv - Genomics 2024Quote: ... amplification of the target USH2A region using gDNA of a human heterozygous USH2A:c.7595-2144A/G patient and subsequent cloning into the pSPL3 backbone vector by NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs). The intronic sequence encompassing the USH2A:c.7595-2144 location was introduced in the recipient pSPL3 exon trapping vector ...
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bioRxiv - Molecular Biology 2021Quote: Myc-Tag (9B11) antibody (NEB 2276 S) and mouse IgG (Santa Cruz sc-2025 ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed with TBS-T and then incubated with an anti-rabbit horseradish peroxidase conjugated secondary antibody (New England Biolabs, 1:10,000 in 5% skim milk in TBS-T) for 2h ...
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...
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bioRxiv - Molecular Biology 2022Quote: ... Butyryl-Histone H3 (Lys 23) mouse mAb (PTM Biolabs, SKU: PTM 307), Butyryl-Histone H4 (Lys 8 ...
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bioRxiv - Genomics 2020Quote: Mouse NIH/3T3 and human Jurkat DNA were from NEB (Ipswich, MA), and XP12 phage DNA was obtained from Dr ...
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bioRxiv - Molecular Biology 2022Quote: ... we used NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E7405), NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765 ...
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bioRxiv - Genetics 2023Quote: ... using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB E7405) or the NEBNext Poly(A ...
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: To obtain pDONR221-APEX.Tango1, the GFP sequence in pT-SP-G-Tango1 (Liu et al., 2017) was removed through NheI (New England Biolabs, cat # R3131L) and SpeI (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were clarified by centrifugation at 10,000 g for 12 min and Endoglycosidase H (Endo H; New England Biolabs, Ipswich, MA, USA) digestion was performed with each sample ...
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bioRxiv - Neuroscience 2020Quote: ... and reverse primer (5’-AAG ATT CTC GAG ATG GTG ATG GTG ATG G-3’) were combined using the Q5 site-directed mutagenesis protocol (New England Biolabs, Ipswitch, MA). The resulting plasmid ...
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bioRxiv - Immunology 2020Quote: ... G and C and lower case ‘g’ represents RNA bases) and the uracil-containing primers subsequently removed by treatment with UDG (NEB, Hitchin, UK). TRA and TRB sequences were amplified using a pair of 5’ ‘step-out’ primers specific for the SMART oligo (long 5’ primer - CTA ATA CGA CTC ACT ATA GGG CAA GCA GTG GTA TCA ACG CAG AGT ...
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bioRxiv - Genomics 2020Quote: ... Approximately 1 µg of fragmented DNA was used for each library preparation using the NEBNext® DNA Library Prep kit (New England Biolabs, UK); DNA-libraries were multiplexed with NEBNext Multiplex Oligos for Illumina kit (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... Pooled samples were clarified by centrifugation (10 000 g, 15 min) and treated with DNase I and RNase If (NEB, Great Britain) for removing of naked out-capsid nucleic acid ...
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bioRxiv - Microbiology 2022Quote: The chimeric VSV variant with 3CLpro instead of the intergenic region between G and L was cloned via Gibson assembly (NEB, Ipswich, USA) (15) ...
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bioRxiv - Biochemistry 2023Quote: ... in vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer with 5 μg RNA substrate (1.8 knt. Same substrate of MTase-Glo experiment) and 1 unit RNase Inhibitor (New England Biolabs Inc., Massachusetts, USA), 1 μM methyltransferase or no enzyme as a negative control ...
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bioRxiv - Genetics 2023Quote: ... 10 µg of the K3L variant library was digested with 5 µL of BstEII-HF and 5 µL SacI-HF (NEB Cat#R3156S) in a 50 µL reaction to generate a linear insert fragment of approximately 1130bp ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µg of genomic DNA was digested with one of few combinations of restriction enzymes (Table S5, all enzymes from NEB, Ipswich, MA). Digested genomic DNA was ligated using T4 ligase (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... custom-made rabbit polyclonal anti-AcK142 or anti-AcK226 (Ez Biolabs) Abs were incubated overnight at 4 °C with the lysate from mock vs ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids made in this study were constructed using G-blocks™ and/or PCR products and assembled using NEBuilder® HiFi DNA Assembly Master Mix following manufacturer’s protocol (NEB, Ipswich, MA). Polymerase chain reactions were performed with Q5 DNA Polymerase (NEB ...
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bioRxiv - Evolutionary Biology 2019Quote: ... cDNAs were synthesised from 1 μg of total RNA in a 20 μL reaction volume using 200 U of Protoscript II reverse transcriptase (#M0368S; NEB, Ipswich, MA, USA), oligo (dT17 ...
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bioRxiv - Immunology 2023Quote: ... 1μg total RNA was used for reverse transcription with the NEB Template Switching Reverse Transcriptase Enzyme Mix (New England Biolabs, Ipswich, MA, USA) according to the recommendations of the manufacturer ...
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bioRxiv - Bioengineering 2023Quote: ErCas12a (1 μg) or LbCas12aU (1 μg) protein and 1 μg crRNA were assembled in 1X Nuclease Reaction Buffer (New England BioLabs, Ipswich, MA, USA) at room temperature for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... 2-8 µg of HMW DNA was diluted in 1x CutSmart Buffer and 6 µl of Quick CIP enzyme (New England Biolabs, catalog no. M0508) was added followed by incubation of the reaction at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 μg RNA was used to isolate poly(A)-enriched RNA with NEBNext® Poly(A) mRNA Magnetic Isolation Module (NE Biolabs, Ipswich, MA) that was used to prepare RNA sequencing libraries with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NE Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNAs were capped co-transcriptionally during the T7 RNA polymerase reaction by decreasing GTP to 0.125 mM with addition of 2.5 mM cap analog (G-cap, NEB S1407S; A-cap, NEB S1406S).
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bioRxiv - Biochemistry 2020Quote: ... Primary antibodies used were: New England Biolabs (NEB), Hitchin ...
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bioRxiv - Genomics 2019Quote: ... mouse NIH/3T3 and human Jurkat DNA were from NEB (Ipswich, MA, USA). E14 genomic DNA was extracted with a DNeasy Blood and Tissue Kit (QIAGEN ...
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bioRxiv - Genetics 2020Quote: ... following rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB).
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bioRxiv - Developmental Biology 2023Quote: Regulatory elements were amplified from mouse genomic DNA with Q5 polymerase (NEB, M0491) using primers listed in Table S8 and cloned into pGL4.24[luc2P/minP] (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-SUZ12 (NEB, 3737), Anti-ASXl1 (Cell Signaling ...
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bioRxiv - Immunology 2019Quote: ... anti-LC3β (NEB, 3868), anti-calnexin (#2433 ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-SNAP (NEB) antibodies ...
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bioRxiv - Systems Biology 2023Quote: ... and anti-MBP (NEB) monoclonal antibodies before proceeding ...
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bioRxiv - Plant Biology 2023Quote: ... and anti-MBP (NEB) antibodies.
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bioRxiv - Genetics 2024Quote: ... Anti-rabbit (#7074, NEB) and anti-mouse (#7076 ...
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bioRxiv - Immunology 2022Quote: The antibody expression vectors for each recombinant antibody (VH + VL-L/k) were transfected together with a Transposase vector (Hera BioLabs, USA). Cells were selected with Hygromycin B (H3274 ...
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bioRxiv - Zoology 2023Quote: ... The recombinant clones containing these constructs were then grown overnight in LB liquid media containing 25 μg/ml of Zeocin™ antibiotic and plasmid DNA isolation was isolated using the Monarch® Plasmid Miniprep Kit (New England Biolabs, Whitby, ON). Base-pair accuracy and orientation of the receptor constructs were confirmed by Sanger sequencing (Center for Applied ...
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bioRxiv - Genetics 2024Quote: ... Cells were then pelleted by centrifugation at 500 x g for 10 min and washed with 200 µL of 1.5X DpnII reaction buffer (New England Biolabs Ipswich, MA cat no. R0543S), pelleted again and then resuspended in 300 µl of cold 1.5X DpnII reaction buffer (NEB cat no ...
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bioRxiv - Microbiology 2021Quote: ... Nitrocellulose membrane-transferred proteins were incubated with anti-PfGet3 or anti-MBP (NEB), probed with corresponding HRP-conjugated secondary antibodies and developed by chemiluminescence.
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bioRxiv - Neuroscience 2022Quote: ... and probed with p62 antibody (NEB D5L7G, 1:800) and streptavidin-594 (Biolegend 405240 ...
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bioRxiv - Neuroscience 2023Quote: ... TCF/LEF family antibody sampler kit (New England BioLabs), NFAT1 (New England BioLabs) ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids encoding mouse Nav1.2 (NP_001092768.1) and Nav1.6 (NP_001070967.1) were cloned by Gibson Assembly (NEB) with synthetic gBlocks gene fragments (Integrated DNA Technologies) ...
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bioRxiv - Cell Biology 2022Quote: Mouse phogrin was obtained from the IMAGE consortium (clone BC_133678) and CLIPf from NEB. The fusion protein was generated by standard molecular cloning techniques ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse Adnp was amplified from pENTR223.1-Adnp using Q5 High Fidelity DNA Polymerase (NEB) and primers containing 6xHis tag to insert it into the C-terminal region of Adnp ...
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bioRxiv - Plant Biology 2020Quote: ... and pan-anti-acetyllysine (pan-anti-Kac, PTM Biolabs, China, Catalog No. PTM-101) were used in this study ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated with custom-generated anti-AcK142 and anti-AcK226 Abs (EZ BioLabs) at a dilution of 1:200 in PBST for 1 h at 37 ⁰C ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-Snail (NEB, 3879S). Secondary antibodies were purchased from CST ...