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Citations for New England Biolabs :
351 - 400 of 672 citations for Mouse Anti Staphylococcus Aureus Enterotoxin G Antibody SEG 16 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... mouse α-myc (New England Biolabs, clone 9B11, 1:2,000), rabbit α-pol-I largest subunit 15 (1:100 ...
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bioRxiv - Genetics 2024Quote: ... and mid-range PFG ladder (for mouse samples) (NEB Inc.) and 1 kb ladder (GeneDirex Inc.) ...
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bioRxiv - Plant Biology 2020Quote: ... 2 µg of RNA for each sample was treated with DNase I at 37°C for 10 minutes (New England Biolabs) and 0.5 µg was used for reverse transcription with the GoScript Reverse Transcription Mix ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 15,000 x g for 30 min at 4°C and the clarified extract was loaded onto a chitin resin (NEB) column pre-equilibrated with column buffer for purification at RT ...
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bioRxiv - Biochemistry 2022Quote: 4µg of pPNL6 vector in Cut Smart buffer was digested using 1µl of NheI HF and BamHI HF (NEB Biolabs) at 37°C for 1h ...
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bioRxiv - Genomics 2019Quote: ... Bead-nuclei were pelleted for 2 min at 2500 g and resuspended in 189 µL blunting solution (1× T4 DNA ligase buffer (NEB), 0.25 mM dNTPs ...
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bioRxiv - Genetics 2019Quote: ... mRNA was purified from 1 μg of total RNA (≥100 ng/μL) using NEBNext Poly(A) mRNA Magnetic Isolation Module for Illumina (New England Biolabs). Sequencing libraries were generated using NEBNext Ultra II RNA Library Prep with Sample Purification Beads for Illumina (New England Biolabs ...
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bioRxiv - Biophysics 2019Quote: ... uncleaved fractions and 3C protease were removed by Ni-chelated sepharose and the G protein was dephosphorylated by lambda protein phosphatase (NEB), calf intestinal phosphatase (NEB) ...
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bioRxiv - Bioengineering 2019Quote: ... and 21 µg of gRNA transcripts produced by in vitro transcription of oligonucleotides (HiScribeTM T7 High Yield RNA Synthesis kit from NEB). Plugs are then treated overnight at 50°C with 10 µL of proteinase K ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was collected after a spin at 16000 g for 30 min and incubated with GFP-Trap (Chromtek) or MBP magnetic beads (NEB) for 3 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2020Quote: ... single-stranded cDNA was synthesized from 10 µg of RNA using 100 pM random hexamer primer (Integrated DNA Technologies) and M-MuLV Reverse Transcriptase (New England Biolabs). After reverse transcription ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was cleared from cell debris by centrifugation at 50000 g for 1 hour followed by incubation with Amylose resin (NEB) for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μg of tissue or cell lysates were treated with or without 2 μL Lambda Protein Phosphatase (LPP) (New England Biolabs) at 30 °C for 15 min (30 min for HEK-293E lysate ...
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bioRxiv - Microbiology 2022Quote: ... and poly(G) tails were added at the 3’ end of the purified ssDNA by terminal transferase (New England Biolabs). The double-stranded DNA was then synthesized and amplified by PCR from the poly(AG)-tailed ssDNA using the primers BLV-F2 and NV-oligo-dT-ADP1 and Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... or used as a template to generate chimeric fimbrial gene clusters containing different G adhesins using the T4 DNA ligase (NEB). PCR fragments containing G adhesin genes were obtained using primer pairs CMD2171_F and CMD2172_R (for papG class I from strain J96) ...
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bioRxiv - Cell Biology 2020Quote: ... The internal EcoRI site was disrupted by introducing at silent mutation at nt1283 (G to A) using Q5 site directed mutagenesis (NEB) and mTimm50-mRFP was cloned in to pQCXIN (Clontech #631514 ...
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bioRxiv - Genomics 2019Quote: ... One million nuclei aliquots were pelleted by 1,000 x g at 4°C for 10 min and resuspended in 200 µl of GpC methyltransferase M.CviPI (NEB M0227L) reaction containing 1X GC Reaction Buffer ...
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bioRxiv - Immunology 2020Quote: ... 1μg RNA from each sample was used for mRNA enrichment using NEBNext Poly(A) mRNA magnetic isolation module (E7490S, NEB). RNA-seq libraries were constructed using the purified mRNA samples with NEBNext Ultra II Directional RNA library Prep Kit for Illumina (E7760S ...
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR products as well as Borrelia shuttle vector pBSV2-G were digested with XmaI and XbaI and the resulting PCR product and pBSV2-G backbone were ligated together using T4 DNA Ligase (New England Biolabs), followed by subsequent transformation into E ...
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bioRxiv - Biophysics 2022Quote: ... The reaction was buffer exchanged in a Microspin G-50 Column (Cytiva) before treatment with Quick Calf Intestinal Phosphatase (CIP, NEB). The plasmid was digested with AgeI ...
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bioRxiv - Microbiology 2022Quote: ... Proteins from the supernatant at 2 mg/mL were used for the Co-IP assay using Protein G magnetic beads (New England Biolabs) and a mouse monoclonal anti-HA antibody (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... and subsequently cloned via Gibson Assembly in the pRRL-EF1a-XhoI-IRES-BlastR plasmid (gift from G. Superti-Furga) using the NEBuilder HiFi DNA Assembly Mix (New England Biolabs). The CRBN/VHL WT and point mutant plasmids were used for lentivirus production and subsequent transduction in RKO CRBN-/- and VHL-/- clones respectively.
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bioRxiv - Biochemistry 2023Quote: ... 100 μg of pSCW01 plasmid was treated with 1.5 units/μg of site specific endonuclease Nt.BstNBI (New England Biolabs, Ipswitch, MA) and 100X molar excess of displacer oligonucleotides (DNA197-199 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The small RNA library preparation was done with 1 µg of total RNA used as input into the NEBNext Small RNA Library Prep Set for Illumina (New England BioLabs) and an AMPure XP Bead (Beckman Coulter ...
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bioRxiv - Biochemistry 2022Quote: ... 50 μg of supercoiled pUBER plasmid was treated with 1 unit/μg Nt.BbvCI in 1 × Cutsmart buffer (New England Biolabs, USA) at 37°C for 4 h ...
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bioRxiv - Genomics 2023Quote: ... Cells were then spun down at 500 g for 5 minutes and washed with 900 μL of NEBuffer 2.1 (New England Biolabs #B7202). The cells were pelleted (500 g for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... double and triple N>G mutations were introduced in the parental constructs by using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturers protocols and using custom designed primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 µg of purified DNA (PCR product after 2nd round PCR after selections) was incubated with PstI-HF (New England Biolabs) at 37 °C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation (15 min, 12000 x g) and 100 units of λ-phosphatase (#P0753, New England Biolabs) were added to the supernatants ...
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bioRxiv - Cell Biology 2023Quote: ... myc-tagged AP3B1 immunoprecipitated and immobilised on Protein G-sepharose beads was incubated with CK2 (250 units; New England Biolabs) for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... reverse 5’-GTGGCC CTCGAG TCA GTG AGT TTC ATG TTG G-3’ and then purified using the Monarch PCR plus DNA purification kit (NEB). The purified PCR product was digested by KpnI and XhoI (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... and 5’-ACG CGT ACT AGT CGA TCG CTT GTA CAG CTC GTC CAT G-3’ (reverse primer) and using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... a five-fold molar excess of oligonucleotides bearing either 8-oxo-G or G was mixed with the phage-extracted circular single-stranded DNA in 1=×=Phusion HF Buffer (New England BioLabs). After denaturation at 90=°C for 2=min followed by rapid cooling ...
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bioRxiv - Plant Biology 2024Quote: ... and a 18-nucleotide random barcode (VNNVNNVNNVNNVNN; V = A, C or G) into the upstream Golden Gate site using the restriction enzyme BbsI-HF (NEB). Next ...
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bioRxiv - Cell Biology 2024Quote: ... was amplified from mouse cDNA by polymerase chain reaction (NEB, M0492L) using primers below:
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bioRxiv - Genomics 2020Quote: ... Nuclei were pelleted at 1,000 x g for 5 min at 4°C then resuspended in 450 μl of 1X NEBuffer 3 (NEB, cat. # B7003S). Primary restriction enzyme digestion of intact nuclei was carried out overnight at 37°C using 50,000 U of DpnII (NEB ...
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bioRxiv - Genomics 2020Quote: ... A subsequent PCR was then repeated and 1ug of the amplified g-block was then in vitro transcribed by T7 RNA polymerase (NEB, M0251) for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... and 50 µg/ml Proteinase K) at 65 °C for 12 hrs and purified with Monarch DNA Cleanup Columns (New England Biolabs, T1030L). For ALaP-qPCR analysis ...
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bioRxiv - Microbiology 2020Quote: ... 2 μg of the extracted genomic DNA was digested for 4 h at 37☐ using 10 U MmeI (New England Biolabs). The DNA was then immediately dephosphorylated by treatment with 1 U calf intestine alkaline phosphatase (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA (1 μg) from each sample was used for library preparation with NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England BioLabs) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Genomics 2021Quote: ... 1μg of total RNA per sample was processed using the NEBNext Ultra II Directional RNA library prep kit from NEB (E7760L) utilising the NEBNext Poly(A ...
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bioRxiv - Immunology 2020Quote: mRNA capping reaction was performed with purified IVT mRNA using 3’-O-Me-m7G(5’)ppp(5’)G RNA Cap Structure Analog (NEB, USA). The reaction condition was followed according to supplier’s manual ...
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bioRxiv - Molecular Biology 2020Quote: ... was linked to the free hydroxyl group at the 3’-end of transcripts (1 □g of total RNA) by T4 RNA ligase 1 (NEB M0204) in the presence of 15% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... GT GGT ATT GCC GAC GGA G) and the combination of both were generated using the Q5® site-directed mutagenesis kit (NEB) (lower case letters highlight the amino acid change) ...
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bioRxiv - Biochemistry 2023Quote: ... and ATF4 5ʹ UTR-nLuc-3XFLAG mRNAs were co-transcriptionally capped with the 3’-O-Me-m7G(5ʹ)ppp(5ʹ)G RNA Cap Structure Analog (NEB # S1411S). All viral IRES nLuc-3XFLAG mRNAs were co-transcriptionally capped with the A(5ʹ)ppp(5ʹ)G RNA Cap Structure Analog (NEB # S1406S).
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bioRxiv - Biochemistry 2023Quote: ... All viral IRES nLuc-3XFLAG mRNAs were co-transcriptionally capped with the A(5ʹ)ppp(5ʹ)G RNA Cap Structure Analog (NEB # S1406S).
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bioRxiv - Molecular Biology 2023Quote: ... from 1 µg of total RNA and library preparation was conducted with the NEBNext® Ultra RNA Library Prep Kit for Illumina (NEB) with Dual Index Primers (NEBNext® Multiplex Oligos for Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.2-2.1 μg were used as a template to generate a sequencing library with the NEBNext Ultra II Directional PolyA mRNA (NEB, E7760S). The Advanced Sequencing Facility of the Francis Crick institute used an Illumina HiSeq 4000 to perform single end 1 × 75 bp sequencing.
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bioRxiv - Plant Biology 2023Quote: ... 7 µg (DNA mass) of NCPs (with 188bp NPS) or free DNA was incubated with 5 Kunitz units of MNase (NEB M0247) in a reaction buffer (30 mM Tris pH 8.0 ...