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Citations for New England Biolabs :
401 - 450 of 771 citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... IVT gRNA was produced from SFV infectious clone plasmid and treated with 10 U of DNase1 (RNase-Free; NEB) for 30 min at 37°C before purification with the RNeasy MiniElute Cleanup kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 2018) was used to clone the Acr genes of interest upstream of aca1 by Gibson Assembly (New England Biolabs). The resulting vectors were used to transform PA14 ΔCRISPR and the strains were infected with WT DMS3m::gent35 cassette ...
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bioRxiv - Cell Biology 2021Quote: ... The targeting genomic PCR in CCR5-mutated clones was performed using Q5 Hot Start High Fidelity DNA polymerase (NEB) with the following primers ...
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bioRxiv - Cell Biology 2022Quote: ... PCR was performed using pBluescript SK+ CPES_V5 clone as template with appropriate sense and antisense primers (Table S1) and Phusion polymerase (NEB). The PCR product was digested with DpnI to remove templates followed by transformation into DH5α competent cells ...
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bioRxiv - Cell Biology 2022Quote: ... PCR was performed using pBluescript SK+ CPES_V5 clone as template with appropriate sense and antisense primers (Table S1) and Phusion polymerase (NEB). The PCR product was digested with DpnI to remove templates followed by transformation into DH5α competent cells ...
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bioRxiv - Immunology 2022Quote: ... T cell clones were thawed and allowed to rest at 37°C in RPMI containing DNAse (5µg/mL, New England Biolabs).
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bioRxiv - Neuroscience 2021Quote: The expression clone for both the peptide of interest and the control was transformed into competent DH5α cells (NEB® [New England Biolabs] 5-alpha Competent E ...
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bioRxiv - Cell Biology 2020Quote: ... R972A mutations were introduced into the aforementioned IRS2 clones using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) with the primers 5’ - AGA TTA TAT GAA TAA GTC CAC TGT CAG ATT ATA TG - 3’ and 5’ - GAC AGT GGA CTT GCC TGG CGA GAG TCT GAA CT - 3’ according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... We then amplified the barcodes from each clone using Q5 High Fidelity 2X Master Mix (M0492, New England Biolabs) with primers specific to our reporter gene (CAS P58-59) ...
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bioRxiv - Biophysics 2024Quote: ... and HCN4 Δ1-185 deletion clones were made using a site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) and a codon-optimized HCN4 plasmid in the pTwist-CMV-WPRE-Neo vector synthesized by Twist Biosciences.
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bioRxiv - Biochemistry 2024Quote: ... All truncated or tagged versions of clones were generated using Q5 high-fidelity DNA polymerase (New England Biolabs #M0515) and NEB-Gibson Assembly (New England Biolabs #E2611S) ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was purified from single cell clones using the Monarch® Genomic DNA Purification Kit (New England Biolabs) and Exon 3 amplified using Q5® High-Fidelity DNA Polymerase (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: The coding region of cDNA clone FI18815 was PCR amplified and cloned in frame into pENTR4 (HiFi assembly, NEB) and then into pPHW using Clonase (Life Tech.) ...
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bioRxiv - Microbiology 2024Quote: ... The JEV cDNA clones for RNA in vitro transcription were generated by cleavage linearization with restriction endonuclease Bsu36I (NEB). The full-length viral RNA was transcribed in vitro from the linearized JEV cDNA templates using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... We then carried out rRNA depletion with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat NEB # E6310S) using the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... SUMO1 and SUMO3 were amplified from human cDNA and cloned in the pTXB1 vector (New England Biolabs) by restriction cloning according to the manufacturers protocol.
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Biochemistry 2024Quote: The cloning of human wild type Mad2 and the mutant Mad2R133A by the USER® method (NEB) into the pRSFDuet-1 vector (71341-3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human RAC1P29S cDNA was cloned into the vector using EcoRI and XhoI restriction enzymes (New England Biolabs) and ligated using T4 ligase (New England Biolabs) ...
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bioRxiv - Developmental Biology 2022Quote: Complementary DNA (cDNA) encoding for Human CD36 (RC221976) was cloned using Hifi DNA assembly kit (NEB E5520S) into the pJFRC-MUH vector (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... the promoter EF1α and human DGCR8 sequences were removed using the restriction enzymes FseI and BsBtI (NEB). After ligation ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was removed using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat; New England Biolabs), and the rRNA-depleted RNA was used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: The chimeric CrPV-aNCV infectious clone was derived from the full-length CrPV infectious clone (pCrPV-3; Accession KP974707) (35) using Gibson assembly (New England BioLabs), per the manufacturer's instructions ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... The human MRLC2 gene from pCS2+-MRLC2-GFP 47 was used for creation of hsp70:MRLC-mKate2 construct which was then used as template for construction of MRLC2-mKate2 middle entry clone (pME) by PCR using Phusion polymerase (New England Biolabs) and primers with ATT recombination site (shown in lower case) ...
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bioRxiv - Genomics 2020Quote: We used the prepared DNA of each clone and the wild type gene for amplification by PCR with Q5 polymerase (NEB) using primers which included the minION barcodes adapters ...
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bioRxiv - Plant Biology 2021Quote: ... and ligated together into the gateway compatible Gene Entry Clone (GEC) plasmid (Wang et al., 2013) by using Gibson Assembly (New England Biolabs). RBP47B-DENDRA2 was cloned into pEarleyGate 100 (Earley et al. ...
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bioRxiv - Neuroscience 2020Quote: ... each round of evolution also included a library created by gene-shuffling the selected clones using the staggered extension PCR method [20] and Taq polymerase (New England Biolabs). The amplified DNA fragments were inserted into the constitutive bacterial expression vector pNCS (gift from Nathan Shaner ...
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bioRxiv - Molecular Biology 2020Quote: ... This was cloned into the pLAI-JRFL molecular clone between BamHI and SfiI restriction sites using T4 DNA ligase (NEB), thus replacing WT env ...
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bioRxiv - Developmental Biology 2022Quote: ... Clones identified as edited had the target ends amplified by PCR with high-fidelity Taq polymerase (Pfusion HF; NEB, M030) and sent for Sanger sequencing (Genewiz ...
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bioRxiv - Cancer Biology 2022Quote: ... OFP positive bulk populations were single-cell cloned in 96-well plates and a total of 12 clones screened for mismatches by T7E1 assay (New England Biolabs). Briefly ...
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bioRxiv - Genomics 2022Quote: ... Full-length L1s were then reconstructed by combining PCR-mutation free fragments from different clones using restriction enzymes (New England Biolabs) recognizing the L1 sequence ...
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bioRxiv - Genetics 2022Quote: ... L1 elements were then reconstructed by combination of non-mutated fragments from different clones using restriction enzymes (New England Biolabs) cutting within the L1 sequence.
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bioRxiv - Synthetic Biology 2020Quote: ... U.S.A.).These respectively were used to clone the genes into storage plasmids (level I) by Golden Gate assembly with BsmBI and T7 DNA ligase (M0318L, NEB) for subsequent assembly into expression cassette-bearing plasmids (level II ...
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bioRxiv - Immunology 2020Quote: ... Mutations inducing amino acid substitutions in the vif region of infectious molecular clones or in the APOBEC3 expression constructs were introduced using Q5 site-directed mutagenesis kit (NEB). The presence of the desired mutations was confirmed by Sanger sequencing.
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bioRxiv - Plant Biology 2022Quote: ... Individual clones were selected and confirmed via colony PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA) with primers 1BF and 8R which were designed to amplify the coiled coil and NB-ARC domain coding sequences of MRI-R1 ...
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bioRxiv - Neuroscience 2022Quote: ... The 5’ and 3’ arms (5 to 8 kb) were amplified from a C57Bl/6 BAC clone by PCR using Q5 polymerase (New England Biolabs) and inserted into a cloning vector that contains frt-flanked SV-Neo for positive selection and Pgk-DTA and HSV-TK genes for negative selection (Jarvie et al. ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA from 12 of the clones was extracted and mutations were verified by PCR using OneTaq polymerase (New England Biolabs) with the appropriate primers (supplementary methods ...
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bioRxiv - Neuroscience 2024Quote: ... The template for the sst1.1 probe was a cDNA clone in a pSPORT1 vector (Argenton et al., 1999) linearized by digestion with SalI (R3138L, New England Biolabs).
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bioRxiv - Genomics 2023Quote: ... the SNAP/Halo-positive clone cultured on coverslips (Matsunami Glass) were treated with HaloTag Oregon Green and SNAP-Cell TMR-Star (New England Biolabs) according to manufacturers’ protocols and fixed with 4% PFA in PBS at room temperature for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... and CUT&RUN samples from two replicate (two independent CRISPRi clones) using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645, NEB). The manufacture’s protocol was employed with the following changes ...
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bioRxiv - Cell Biology 2023Quote: pTO-GFP-SNX17 was used to generate an shRNA resistant version of GFP-SNX17 by introducing three silent mutations into the target sequence of the SNX17 shRNA clone 1 construct using the Q5-site directed mutagenesis kit (New England Biolabs). Specifically ...
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bioRxiv - Neuroscience 2023Quote: ... Fragments were then assembled into a single tln-1 gDNA clone in a pCR8 vector using HiFi DNA assembly (NEB). Using gateway technology (LR clonase II ...
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bioRxiv - Microbiology 2024Quote: ... SINV-REP construct derived from pToto64 clone encoding SINV non-structural proteins and a reporter luciferase tag was linearized using SacI (New England Biolabs), followed by in vitro transcription using SP6 RNA polymerase (New England Biolabs) ...
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bioRxiv - Genetics 2024Quote: ... Golden Gate assembly was used to clone sgRNAs into the desired backbone using BsaI (BsaI-HF v2, New England Biolabs, NEB) (26) ...
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bioRxiv - Microbiology 2024Quote: ... SIVsmm Vpr and Nef expression constructs were described before20,51 Chimeric HIV-2 and SIVsmm infectious molecular clones as well as CG mutants thereof were generated using Gibson assembly (NEB) and cloned into pCR XL TOPO or pBR vector using NotI/MluI restriction sites using primers listed in Table 1 ...
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bioRxiv - Plant Biology 2024Quote: ... Mutant versions of the promoter were generated from the wild type (WT) clone using the Q5 Site-Directed Mutagenesis Kit (NEB) and mutagenic primers (Table S1) ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... of the Oprl1 gene were amplified from a C57BL/6 BAC clone by PCR using Q5 Polymerase (New England Biolabs) and cloned into polylinkers of a targeting construct that contained IRES-mnCre:GFP ...
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bioRxiv - Biochemistry 2024Quote: The TSV-IRES mRNA 6741-6990 was obtained by T7 in-vitro transcription from a PCR product amplified from the plasmid containing the mRNA clone and the transcript was purified by using RNA purification kit (NEB) (4).
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bioRxiv - Genetics 2024Quote: ... to clone upstream of the stuffer sequence in the puORF-Check3 plasmid backbone using the NdeI restriction site (NEB HiFi). Mutated sequences of each 5’UTR were cloned in the same manner as well ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...