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Citations for New England Biolabs :
451 - 500 of 771 citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was depleted of ribosomal RNAs using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) ...
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Evaluation of the OsTIR1 and AtAFB2 AID systems for chromatin protein degradation in mammalian cellsbioRxiv - Molecular Biology 2021Quote: ... mCapH2 (Gene ID: 52683) genes were PCR amplified from human and mouse genomic DNA with Q5 polymerase (NEB). The lengths of homology arms are featured in electronic supplementary material Table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... Snap-tagged human β1AR and β2AR constructs were labeled with Snap-cell 647 SiR (New England Biolabs, S9102S) at 1:1000 concentration for 20 min at 37°C in DMEM without phenol red supplemented with 30 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: Codon-optimized human TDP-43-His LIC-A constructs were transformed into T7 express (New England Biolabs, #C3029J) E ...
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bioRxiv - Cell Biology 2024Quote: ... which was amplified from human cDNA library and cloning into pEGFP-N2 using Gibson assembly method (NEB E2611L). A stop codon was inserted before EGFP in pEGFP-N2 vector to clone untagged APMAPFL ...
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bioRxiv - Molecular Biology 2023Quote: ... Removal of rRNA was carried out by use of the NEBNext rRNA Depletion Kit Human/Mouse/Rat (NEB) followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) (New England Biolabs) following the manufacturer’s protocol (including RNase H and DNase I digestions) ...
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bioRxiv - Cell Biology 2024Quote: ... Full length human NHSL1-A1 including Exon 1a and excluding exons 3 and 6 was generated by NEB HIFI assembly using a synthetic DNA fragment (gBlocksTM ...
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bioRxiv - Cell Biology 2024Quote: Human RABs were PCR amplified with the Phusion Hot Start Flex 2X master mix (M0536; New England Biolabs) from a cDNA library generated from mixed total RNA extracted with Trizol reagent (15596026 ...
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bioRxiv - Genomics 2020Quote: Primer-walk PCRs were performed with edited single-cell clones to identify aberrant PCR products with OneTaq 2x Master Mix (NEB M0486L) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: The expression clone for both the peptide of interest and the control was transformed into competent DH5α cells (NEB® [New England Biolabs] 5-alpha Competent E ...
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bioRxiv - Immunology 2020Quote: Diabodies were produced from scFv clones by PCR of the VL and VH domains using Q5 DNA polymerase (NEB, Cat: M0491S) to shorten the amino acid linker between the domains to SSGGGGS ...
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bioRxiv - Cell Biology 2024Quote: ... The WT PACT expression vector was used as template for constructing p38 mutant PACT clone by using Q5 site-directed mutagenesis kit (New England Biolabs, E0554S). Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged MyoID was generated for this study by cloning cDNA clone SD01662 from Drosophila Genomics Research Center (DGRC) into a pUAST vector using NEBuilder HiFi DNA Assembly Master Mix (NEB # E2621L). Plasmids encoding different Ds-ICD motif deletion constructs with a C-terminal V5-tag were created in this study ...
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bioRxiv - Genomics 2023Quote: ... The insert size of the BAC clones was assessed using the FastNot I restriction enzyme (New England Biolabs, Ipswich, MA, USA) and analyzed by pulsed field gel electrophoresis ...
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bioRxiv - Biophysics 2023Quote: ... colonies carrying correct clones were identified by colony PCR using OneTaq® Hot Start Quick-Load® 2X Master Mix (NEB), and plasmids were purified from overnight culture in LB containing 150 μg/ml ampicillin at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... a plasmid containing a full infectious clone of RV-M33 was linearized then added to an in vitro transcription reaction with Sp6 (New England Biolabs, M0207L). The resulting RNA was purified then polyadenylated (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... Golden Gate assembly was used to clone sgRNAs into the desired backbone using BsaI (BsaI-HF v2, New England Biolabs, NEB) (26) ...
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bioRxiv - Molecular Biology 2024Quote: ... Single-cell clones were isolated and screened for insertion of the HA tag by PCR and restriction digestion with NdeI (NEB, R0111S). The insertion was also verified by nanopore sequencing of the unrestricted PCR amplicon.
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bioRxiv - Evolutionary Biology 2024Quote: ... All clones were selected by Ampicillin resistance and their composition was confirmed by enzyme digestion with NotI (New England Biolabs, R0189) and Sanger sequencing (Oxford Zoology service ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ triphosphates on the synthesis products were converted into 5’ monophosphates: samples were treated with recombinant shrimp alkaline phosphatase (NEB) (10 µl reaction in 1× NEB buffer 2.1, 37°C 30 min, 65°C 5 min), these reactions were made up to 15 µl including 0.5 µl 10× NEB 2.1 ...
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bioRxiv - Molecular Biology 2021Quote: Myc-Tag (9B11) antibody (NEB 2276 S) and mouse IgG (Santa Cruz sc-2025 ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed with TBS-T and then incubated with an anti-rabbit horseradish peroxidase conjugated secondary antibody (New England Biolabs, 1:10,000 in 5% skim milk in TBS-T) for 2h ...
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bioRxiv - Genetics 2022Quote: Candidate enhancers were amplified from human genomic DNA using Q5 Hot-Start High-Fidelity DNA polymerase (New England Biolabs) and directionally cloned into the pGL3-Promoter plasmid (pGL3-P ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Synthetic Biology 2022Quote: ... sgRNA expression plasmids were constructed via digesting the sgRNA backbone plasmids that contained a human U6 promoter and a Cas-nuclease’s corresponding sgRNA scaffold sequences using BsmB I or Bbs I endonuclease (NEB) and then ligated the oligonucleotide duplexes into this cut backbone ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... Leishmania DNA (0.0017 ng/μL) was then enriched from the human DNA (25ng/μL) using NEBNext Microbiome DNA Enrichment Kit (NEB) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs encoding human elongation factor eEF2 were amplified by PCR using Q5 High-Fidelity 2X Master Mix (NEB) with primers (forward primer ...
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bioRxiv - Cell Biology 2023Quote: ... elegans osgn-1 (F30B5.4a, WormBase WS265) and human OSGIN1 were amplified by PCR using Phusion DNA polymerase (NEB, M0530L), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: mRNA was enriched from 100ng DNase treated total RNA using the NEBNext rRNA depletion Kit (human, mouse, rat, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Specific mutations in human PSEN1 were obtained by Q5 site-directed mutagenesis of the pMSCV-puro vector containing wild type human PSEN1.26 We designed primers for Q5 site-directed mutagenesis (New England Biolabs) with NEBaseChanger.neb.com and performed site-directed mutagenesis according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... OMNI ATAC-Seq was performed as previously described with 320,000 human CD4+ T cells (in total) in batches of 100,000 cells per reaction (NEB #M0544S).(26 ...
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bioRxiv - Genetics 2022Quote: ... The sequences of HS5 and NRE were PCR amplified from human genomic DNA using Phusion high fidelity polymerase (NEB). Hg38 genome coordinates and sequences of primers used ...
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bioRxiv - Immunology 2023Quote: ... A 140-500 ng aliquot of total RNA was rRNA depleted using NEB’s Human/Mouse/Rat RNAse-H based Depletion kit (New England BioLabs). Following rRNA removal ...
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bioRxiv - Microbiology 2020Quote: ... were used to clone plasmids using a combination of standard molecular cloning techniques and Gibson Assembly (master mix from New England Biolabs, Ipswich, MA). The plasmid pJS167 (45 ...
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bioRxiv - Genetics 2022Quote: The full-length ccf-1 cDNA clone was generated through PCR using the Q5® High-Fidelity DNA polymerase (New England BioLabs, M0491L) and cloned into the pDEST32 vector containing the GAL4 DNA binding domain (DBD ...
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bioRxiv - Microbiology 2024Quote: ... primers were designed to clone Ceg10 into pET28b using restriction enzymes NdeI and NotI by HiFi DNA Assemby kit (NEB; Table S4), producing the His6x-TEV-Ceg10 vector ...
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bioRxiv - Biochemistry 2023Quote: ... custom-made rabbit polyclonal anti-AcK142 or anti-AcK226 (Ez Biolabs) Abs were incubated overnight at 4 °C with the lysate from mock vs ...
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bioRxiv - Cell Biology 2020Quote: ... The coding sequence for Dendra2 was amplified from a plasmid clone and inserted into this backbone using the Gibson Assembly® Cloning Kit (New England Biolabs, Frankfurt, Germany); the primer sequences are available upon request ...
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bioRxiv - Microbiology 2022Quote: pL5-UV15-TetO-bioA plasmid was produced by PCR amplification of MAB_2688c with 20 bp complementarity to pL5 PTetO Msm PonA1 truncation A-FLAG clone 1106 using Phusion High-Fidelity Polymerase (M0530, NEB, Ipswich, MA, USA), followed by isothermal assembly107 into NdeI (R0111 ...
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bioRxiv - Genomics 2021Quote: Individual clones were assessed for PDCL3P4 knockout by performing a genotyping PCR with Q5® High-Fidelity DNA Polymerase (New England BioLabs cat # M0492S) and primers placed outside of the gRNA cut sites ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR product and pBR43IeG-nef+ (NIH AIDS reporter clone #11349) were then digested with NcoI and XmaI (New England Biolabs, Ipswich, MA, USA). The digested products were separated by 0.8% agarose gel electrophoresis and purified using the Monarch® DNA Gel Extraction Kit (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... Primary antibodies used were: New England Biolabs (NEB), Hitchin ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse antibodies against MBP (NEB E8032L, 1:20,000) were used to detect 2XMBP-tagged proteins and rabbit antibodies against HA (Cell Signaling C29F4 ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length 265 nt 5’ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Immunology 2021Quote: ... PCR products were purified and cloned into human-IgG (Heavy, Kappa or Lambda) expression plasmids(von Boehmer et al., 2016) using the Gibson Assembly Master Mix (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Library preparation from human islets total RNA (200ng) was performed using a NEBNext Low Input RNA Library Prep kit (NEB). Sequencing was performed on a HiSeq4000 using 75 bp paired end reads according to Illumina specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length 265 nt 5′ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...