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Citations for New England Biolabs :
4401 - 4450 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... library preparation was conducted using a NEBNext Ultra RNA Library Prep kit for Illumina (NEB). After cluster generation ...
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bioRxiv - Cancer Biology 2022Quote: ... after which DNA was acquired using the Monarch DNA cleanup kit (NEB, Ipswich, MA, USA), and DNA-based qPCR quantification was done ...
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bioRxiv - Molecular Biology 2022Quote: ... and purification of the PCR fragments was done using the Monarch Gel Purification Kit (NEB). All fragments for plasmids cloning were amplified using Q5 DNA Polymerase (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... Sequencing libraries were prepared using the Next Ultra RNA Library Prep kit (New England Biolabs) with 12 cycles of PCR amplification ...
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bioRxiv - Plant Biology 2022Quote: ... and subsequently introduced into pDC45_dsg vector using Golden Gate Assembly Kit (E1601L, New England Biolabs). The tobacco FT gene was amplified using NtFT-F and NtFT-R primers ...
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bioRxiv - Molecular Biology 2022Quote: RNA from K562 cell lines was extracted using the Monarch Total RNA purification kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared using NebNext Ultra II DNA Library Prep Kit (New England BioLabs) with Methylated Adaptor (New England BioLabs) ...
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bioRxiv - Genomics 2022Quote: ... IVT was performed to generate dsRNA using the T7 High Yield RNA Synthesis Kit (NEB). Template DNA was removed using Turbo DNAse (Ambion ...
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bioRxiv - Microbiology 2022Quote: ... The Monarch DNA Gel Extraction and Monarch PCR and DNA Cleanup kits (New England Biolabs) were used to clean PCR products according to manufacturers instructions ...
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bioRxiv - Biophysics 2022Quote: ... Libraries were quantified with quantitative PCR (NEBnext Library quantification kit, New England Biolabs, Ipswich, USA) and an Agilent Bioanalyzer High Sensitivity DNA chip ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR product was purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs, Inc.). The samples were run on a 1.5% Agarose (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... RNA samples were prepared using the Monarch Total RNA Miniprep kit (New England Biolabs Inc.), followed by a DNAse treatment using the turbo DNA-free kit (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... Reagents and general procedures from the Gibson Assembly Cloning Kit from New England Biolabs (NEB) were used ...
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bioRxiv - Plant Biology 2021Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) and sequenced on an Illumina Hiseq Xten platform ...
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bioRxiv - Neuroscience 2020Quote: RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). To confirm that striatal ChIs or midbrain dopaminergic neurons were collected via LCM ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Microbiology 2020Quote: ... the sequencing library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB), and sequenced with a paired-end 150 bp run on an Illumina NextSeq ...
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bioRxiv - Microbiology 2021Quote: ... libraries were prepared using the Ultra DNA Library Prep kit for Illumina (New England Biolabs). Additional steps of clean-up were performed using AMPure XP beads ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were quantified using NEB Next Libraries Quantification kit for Illumina (New England Biolabs) and multiplexed at equimolarity ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... CA using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, #E7775) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was isolated and purified using the total RNA extraction and purification kit (Monarch, NEB). RNA concentration and integrity were calculated using a Nanodrop and agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2021Quote: ... dsRNA was produced using the HiScribe T7 High yield RNA synthesis kit (New England BioLabs). Templates were PCR products with T7 promoter sequences added to both ends to facilitate bidirectional transcription using the oligonucleotides listed in Table S1 ...
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bioRxiv - Plant Biology 2021Quote: ... prior to small RNA sequencing library preparation using the NEBNext Small RNA kit (NEB; E7330). Libraries were pooled and sequenced by The University of Arizona Genetics Core on an Illumina NextSeq500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... with Luna Universal One-Step Rt-qPCR & Probe One-Step RT-qPCR Kits (NEB, USA), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The Tb927.8.1500 and Tb927.11.4900 mutants were generated using the Q5 site-directed mutagenesis kit (NEB) from the wildtype vector templates ...
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bioRxiv - Molecular Biology 2019Quote: ... Insertion of CBD sequences were performed using the Q5® Site-Directed Mutagenesis Kit (NEB). Chimeras of GLuc were named GLuc-(DENV2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Start codon mutations were generated by site directed mutagenesis (Q5 Site directed mutagenesis kit, NEB).
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bioRxiv - Plant Biology 2021Quote: ... and reverse-transcribed following manufacturer’s recommendations (The ProtoScript Taq RT-PCR Kit, New England Biolabs). Relative mRNA ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... RT-PCR was performed using LunaScript RT SuperMix Kit (New England Biolabs – Catalog No: E3010L). Each Complementary DNA (cDNA ...
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bioRxiv - Microbiology 2020Quote: ... Beads were resuspended in a 100 μL blunt enzyme mix reaction (Quick Blunting Kit, NEB) with half the enzyme concentration of the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The amplified RNA was than purified using Monarch RNA Cleanup Kit (New England BioLabs Inc.) and quantified by RNA HS reagent using Qubit system (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... The puromycin-linked mRNA was then translated using the PURExpress ΔRF kit (New England Biolabs) with the addition of release factors 2 and 3 per the manufacturer’s instructions along with a “Solution A” containing 19 amino acids (-Met ...
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bioRxiv - Microbiology 2020Quote: ... The PCRs followed the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) PCR protocol ...
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bioRxiv - Microbiology 2021Quote: ... final quantification was performed using the NEBNext® Library Quant Kit for Illumina® (NEB) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: We quantified plasma luciferase activity by a BioLux Gaussia Luciferase Assay Kit (NEB, Ipswich, MA) from 10 μL plasma acquired by retro-orbital bleeding ...
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bioRxiv - Biophysics 2021Quote: ... We used the Luna Universal One-step RT-qPCR kit (E3005S, New England Biolabs, MA) to determine the relative abundance of target mRNA between two samples ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted from cells using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs). Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit ...
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bioRxiv - Genetics 2022Quote: ... RNA was generated using the Hiscribe™ T7 Quick High Yield RNA Synthesis Kit (NEB) followed by purification using the RNeasy MinElute Cleanup Kit (Qiagen).
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bioRxiv - Developmental Biology 2022Quote: ... (2019): 1) the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) was used for library preparation ...
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bioRxiv - Developmental Biology 2022Quote: ... Visible bands were cloned using the NEB PCR cloning kit (New England Biolabs, MA, USA) and sanger sequenced ...
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bioRxiv - Developmental Biology 2022Quote: ... The molar library concentration was assessed using NEBNext Library Quant Kit for Illumina (NEB, E7630). Libraries were diluted to 4nM ...
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bioRxiv - Microbiology 2022Quote: ... For library preparation Illumina protocol NEBNext® Ultra™DNA Library Prep Kit (NEB, USA) was used starting from 200 ng DNA ...
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bioRxiv - Systems Biology 2022Quote: ... In vitro transcription (IVT) was performed with the HiScribe™ T7 RNA Synthesis Kit (NEB). For probes in the original protocol ...
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bioRxiv - Immunology 2022Quote: ... The target sequences were cloned into pET16b by ligation using Quick Ligation™ Kit (NEB). After ligation ...
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Recurrent but short-lived duplications of centromeric proteins in holocentric Caenorhabditis speciesbioRxiv - Evolutionary Biology 2022Quote: ... PCR was done on cDNA using Phusion High-Fidelity DNA Polymerase Kit (New England Biolabs) guidelines according to the manufacturer’s recommendations using primers for hcp-3 ...