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Citations for New England Biolabs :
4401 - 4450 of 6282 citations for 6 Quinolinamine 1 ethyl 1 2 3 4 tetrahydro 2 2 4 trimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... cells were then incubated with RNase H (NEB, 1: 50 in 1X RNase H buffer) at 37°C for 4 hours ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we added 1 uL of T4 DNA ligase buffer (New England Biolabs, USA, product #B0202S). Using a thermal cycler (C1000 Touch Thermal Cycler ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction system was as follows: 20 μL volume containing 1× reaction buffer (NEB, B9012S), 5 U/μL T7 RNA polymerase (NEB ...
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bioRxiv - Genomics 2024Quote: ... 1.35 μL of 1 mM dNTPs and 0.8 μL Klenow exo-polymerase (New England Biolabs) were added to fill in the barcode oligo ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of the sgRNA was complexed with 20 pmol recombinant Cas9 endonuclease (NEB # M0646T) and injected into the C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was stopped by adding 1 µL of Thermolabile Proteinase K (NEB, Ipswich, MA) and incubating for 30 min at 37°C then an additional 10 min at 60°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was stopped by adding 1 µL of Thermolabile Proteinase K (NEB, Ipswich, MA) and incubating for 30 min at 37°C then an additional 10 min at 60°C ...
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bioRxiv - Microbiology 2024Quote: ... 0.4 µg μl-1 bovine serum albumin (New England Biolabs® Inc., Ipswich, MA, USA) and 5 µl DNA template ...
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bioRxiv - Genetics 2024Quote: ... A mixture of 20 ng/μL transgene and 70 ng/μL 1 kb ladder (NEB) filler DNA was microinjected into CZ26711 young adult hermaphrodites ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of the step two plasmid library was linearized with SpeI-HF (NEB #R3133) at 37°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... RNA samples (1 µg each) were mixed with 2x RNA Loading Dye (New England Biolabs), denatured at 72 °C for 10 minutes ...
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bioRxiv - Biophysics 2024Quote: ... N-glycosylation was removed by a 1-hour incubation with PNGase F (500 U, NEB) and following digest with sequence-grade trypsin (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of one plasmid was digested overnight with 10 units PacI (New England Biolabs) in 20 μL at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was synthesized from 1 μg of RNA using M-MuLV Reverse Transcriptase (NEB) and random hexamer primer (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% Igepal CA-630 and 5% glycerol) with 1 U/µL murine RNase inhibitor (NEB), 0.1 µg/µL T7 RNA polymerase (purified in-house ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were anti-PDGF-Ra (rabbit, New England Biolabs, 1:400 dilution), anti-APC (clone CC1 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of purified DNA was treated with 10 units Exonuclease V (New England Biolabs) in a 50 μl reaction at 37°C for 30 min to remove ssDNA ...
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bioRxiv - Cell Biology 2023Quote: ... and M-MuLV reverse transcriptase enzyme (200,000 U mL−1, New England Biolabs, Ispwich, USA) was added per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was then probed with the primary anti-MBP antibody (NEB E8032S, 1:10000) diluted in 5% NFDM (overnight ...
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bioRxiv - Biochemistry 2023Quote: Extracted samples (1 μL) were digested with NotI-HF (0.89 unit/μL, New England Biolabs) and ClaI (0.44 unit/μL ...
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bioRxiv - Cancer Biology 2023Quote: ... digested overnight at 37°C with 1 μl 20 U/μl NotI-HF (NEB R3189S) and 1 μl 10 U/μl PmeI (NEB R0560S ...
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bioRxiv - Developmental Biology 2023Quote: ... 7 μL of 2x NEBNext Library Quant Master Mix with 1:100 low ROX (NEB), and 1.5 μL ddH2O ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... the isolated RNA was treated with 1 μl (5 units) RppH enzyme (New England Biolabs) in NEB Buffer 2 (50 mM NaCl ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 1 µg RNA was reverse transcribed to cDNA (LunaScript RT SuperMix, New England BioLabs, USA).
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bioRxiv - Physiology 2023Quote: ... 1 U of Phusion® Hot Start II High-Fidelity DNA polymerase (New England BioLabs), 500 nM of the 27F-DegS primer ...
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bioRxiv - Cancer Biology 2023Quote: ... agarose plugs containing DNA were washed three times with 1× NEBuffer 3.1 (New England BioLabs) with 5-minute incubations ...
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bioRxiv - Biochemistry 2023Quote: ... and ScFv16 in the presence of 25 mU ml-1 apyrase (NEB, Cat. no. M0398S) and 1 µM of individual ligand ...
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bioRxiv - Biochemistry 2023Quote: ... 1.35 μL of 1 mM dNTPs and 0.8 μL Klenow exo-polymerase (New England Biolabs) were added to the annealed product ...
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bioRxiv - Cell Biology 2023Quote: ... using 5 μl of the reaction with 1 μl of loading dye (New England Biolabs). Agarose gel was electrophoresed at 70V until resolution of bands required was reached ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.35 μL of 1 mM dNTPs and 0.8 μL Klenow exo-polymerase (New England Biolabs) were added and incubated at 25 °C for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µL of T4 DNA ligase high-concentration (2,000,000, units/mL, NEB, cat. no. M0202T) was added ...
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bioRxiv - Developmental Biology 2023Quote: ... After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB, M0541S). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... at 37 °C for 1 hour prior to the addition of T4 DNA ligase (NEB). The ligation reaction was incubated at room temperature for 1 hour then transformed into DH5ɑ competent cells.
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bioRxiv - Evolutionary Biology 2024Quote: ... adding 3.5 µL of Cutsmart buffer and 1 µL of ApaLI restriction enzyme (NEB #R0507L) to each sample’s Step 2 PCR product ...
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bioRxiv - Biochemistry 2024Quote: ... EU-labeled cells fixed and permeabilized as above were incubated in 1×CutSmart buffer (NEB) with 4ng/µL RNase A or 5U/µL benzonase ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was generated from 1 µg RNA using LunaScript RT SuperMix Kit (New England Biolabs). qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... the target site was amplified by Taq DNA polymerase:DeepVent polymerase 200:1 U (both NEB) with CRISPR efficiency primers and sequenced ...
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bioRxiv - Genomics 2024Quote: ... amplifications were submitted to cleavage assays using the T7 Endonuclease 1 (T7E1) enzyme protocol (NEB). PCR products of confirmed G2 heterozygous (n = 48 ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were ethanol-fixed and incubated with 1:100 anti-H3K27me3 antibody (NEB, 9733S) as described previously (34) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg/mL of both DNase I and RNase I (New England Biolabs, Ipswich, USA) was added and incubated with the phage at room temperature for 1 hour to digest any non-phage nucleic acid present in the mix ...
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bioRxiv - Cell Biology 2020Quote: ... 3 µg of purified DNA was digested with 10 units I-SceI (NEB, R0694), 10 units I-CeuI (NEB ...
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bioRxiv - Neuroscience 2021Quote: ... with 3’-O-Me-m7GpppG anti-reverse cap analog (ARCA) or ApppG cap (NEB) added at 8:1 to GTP for a capping efficiency of ∼90% ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μg of RNA was resuspended in 6x Loading dye (Biolabs, cat. No B7024S) and separated on a 20% PA gel without urea run at 160 volts at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Precipitated RNA 3’ends were first dephosphorylated by shrimp alkaline phosphatase (SAP) (NEB M0371), and 3’ RNA linker (20 µM ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... 5’-gaattcgatgtgtaggctggag-3’ using Gibson assembly technique (kit was purchased from New England Biolabs), to yield pIJ773-Δspt9 ...
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bioRxiv - Cancer Biology 2019Quote: ... KRAS and BRAF 3’ homology arms using high-fidelity Phusion Polymerase (New England BioLabs). The 5’ homology arm of KRAS spans the region Chr12:25245385-25245994 ...
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bioRxiv - Plant Biology 2020Quote: ... adaptor-ligated fragments were treated with 3 µl of USER® Enzyme (NEB, USA) before selective PCR enrichment using Phusion® High-Fidelity DNA polymerase using Universal and index primers ...
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bioRxiv - Biophysics 2020Quote: ... and 7U/mL Klenow Fragment of DNA Polymerase I (3’-5 exo-) (NEB, M0212). Reactions were incubated at 37°C and incorporation of labeled dCMP was monitored by an acid precipitation assay ...
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bioRxiv - Genetics 2022Quote: ... and ligating into the BbsI ligated U6:3 vector (T4 ligase NEB, BbsI NEB) (S3 Table) ...