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Citations for New England Biolabs :
4301 - 4350 of 6282 citations for 6 Quinolinamine 1 ethyl 1 2 3 4 tetrahydro 2 2 4 trimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... 3 µl of dNTPs (2.5 mM each) and 5 U Klenow exo- (NEB) and incubating for 1 h at 30°C ...
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bioRxiv - Biochemistry 2021Quote: 100 μl extension reactions were performed with Klenow Fragment (3’→5’ exo-) (NEB) (Figures 2B ...
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bioRxiv - Biophysics 2021Quote: ... where GRN-3 was expressed in Escherichia coli SHuffle cells (New England Biolabs) while other GRNs were expressed in Origami 2 DE3 cells (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was supplemented with 3 μl of T4 RNA ligase I (NEB), 1.5 μl of 10X reaction buffer ...
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bioRxiv - Biophysics 2021Quote: ... and poly(A)-tailed at their 3’ ends with poly(A) polymerase (NEB), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... then perform A-tailing with Klenow Fragment (3’-> 5’ exo-) (NEB, Cat. M0212S) provided with dATP ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was removed 3’phosphoryl groups using T4 Polynucleotide Kinase (New England Biolabs) in the buffer (70 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 ng of DNA was used per qPCR reaction (qPCR Master Mix, NEB). PCR was performed using an Applied Biosystems 7500 instrument (software version 2.3) ...
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bioRxiv - Genomics 2019Quote: ... and 3 µl of NEBNext Ultra II End Prep Enzyme Mix (E7545L, NEB) were mixed gently in 1.5 ml Eppendorf tube ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’ phosphorylation and 3’ dephosphorylation were performed with T4 PNK (NEB, Cat: M0201S) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and A-tailed using Klenow 3’-5’exo-enzyme (NEB, Cat. No. M0212). Illumina sequencing library adapters were subsequently ligated to DNA ends ...
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Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... In-line barcoded 3′ adapters were ligated with T4 RNA ligase I (NEB) for 12 hours at 20°C ...
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bioRxiv - Genomics 2022Quote: ... and 3’-adenylated with NEBNext Ultra II End Repair/dA-Tailing Module (NEB). The DNA was then purified with AMPure XP beads (Beckmann Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... pUC19-3’ITR-3xCTCF- rHA was linearized with KpnI and HindIII (NEB, R3104L) and the Neomycin resistance gene was amplified from pEN113 (Addgene ...
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bioRxiv - Genomics 2022Quote: ... and 3 µL 5U/µL DNA Polymerase I Large (Klenow) Fragment (NEB, #M0210V) was added and incubated at 37°C for 45min with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was then ligated to adaptors at the 3’ end by NEB 3’ SR adaptor and 5’ end by T4 RNA ligase ...
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bioRxiv - Genetics 2024Quote: ... the slides were treated with 3 U/µl exonuclease III (New England Biolabs) in 1x NEB cutsmart buffer and incubated at 37°C for 15 min to digest nicked BrdU-positive strands ...
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bioRxiv - Molecular Biology 2022Quote: ... Next the 3’ primer regions were truncated using the BciVI restriction endonuclease (NEB), followed by 2.0x AMPure XP cleanup ...
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bioRxiv - Molecular Biology 2023Quote: Hi-C libraries were treated with 3 U of T4 DNA polymerase (NEB) in the presence of 0.1 mM dGTP (NEB ...
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bioRxiv - Genomics 2023Quote: ... and A-tailed with Klenow Fragment (3’-to-5’ exo-, New Englands Biolabs) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA was radiolabelled at the 3’ end using T4 RNA ligase I (NEB) and [32P] pCp (Perkin Elmer ...
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bioRxiv - Cell Biology 2023Quote: ... which were then degraded by the 5’-3’ ssDNA exonuclease RecJ (NEB, M0264S). After rRNA reduction using the riboPOOL rRNA depletion kit (siTOOLs Biotech ...
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bioRxiv - Genomics 2023Quote: ... Nicked DNA was then extended with 3 U of Vent (exo-) Polymerase (NEB), 100-300 µM dNTPs ...
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bioRxiv - Cell Biology 2024Quote: ... The 3’ homology arm was amplified by PCR (Q5 HiFi polymerase®, NEB), from HCT116 cell genomic DNA using the primers NCAPH2 3’ homology arm forward and reverse ...
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bioRxiv - Molecular Biology 2024Quote: ... The L3 linker was then ligated to the 3′ RNA ends (with NEB HC RNA Ligase in ligation buffer (200 mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2021Quote: ... 6×50 µl PCR reactions were set up using NEBNext Ultra II Q5 master mix (New England Biolabs) with 5 nM oligos as template ...
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bioRxiv - Genetics 2022Quote: ... we PCR amplified NatMX from Addgene plasmid #35121 with the primers listed in Supplementary Table 6 using Phusion Hot Start Flex DNA polymerase (NEB). The NatMX cassette was transformed into the BY strain using the methods described above and transformants were plated onto YPD medium containing clonNAT ...
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bioRxiv - Molecular Biology 2022Quote: ... The final cDNA libraries were amplified (cycle number of 6) using NEBNext Multiplex Oligos for Illumina (NEB, E7335) and purified using SPRIselect size selection beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.6 mM of A, C, and T, NEB-N0446S; 0.1 µM Clean.G; 800 U/ml Bst Polymerase, NEB-M0374L ...
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bioRxiv - Biophysics 2021Quote: ... 25 μL of PCR reaction was mixed with 1 μL of DpnI (NEB, cat#R0176) at 37°C for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Chromatin was digested in situ using five units of restriction enzyme CviKI-1 (NEB, R0710S) every 100.000 nuclei and RELACS custom barcodes (4 bp UMI + 8 bp RELACS barcode ...
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bioRxiv - Microbiology 2019Quote: PCR mixtures were digested for 1 h at 37 °C with DpnI (New England Biolabs) to remove the template plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then stained with 1 µM of SNAP-Surface-AlexaFluor 647 (BG-A647, NEB) in 4% BSA + 0.1% Tween-20 for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the dUTP-containing second strand was digested by adding 1 µL of USER enzyme (NEB) and incubating at 37 °C for 15 minutes ...
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bioRxiv - Genomics 2019Quote: ... bead-nuclei were resuspended in 190 µL RNA ligase solution (1× RNA ligase buffer (NEB), 4.5 µM bridge adapter ...
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bioRxiv - Genomics 2019Quote: ... supplemented with 0.1% Triton X-100 and with 200 µL 1× RNA ligase buffer (NEB) by repeating resuspending/pelleting.
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bioRxiv - Cell Biology 2019Quote: ... 1) Genomic DNA was digested with 200U HindIII-HF (New England BioLabs, prod. number R3104S) at 37°C overnight ...
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bioRxiv - Biophysics 2019Quote: ... 25 µL of PCR reaction was mixed with 1 µL of DpnI (NEB, cat# R0176), 1 µL of T4 PNK (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was applied onto a column with 1 ml of equilibrated amylose resin (NEB). The column with the beads and the extract was incubated on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Synthetic Biology 2019Quote: ... We added 1 µL of murine RNase inhibitor to all in vitro reaction (NEB# M0314S). Each reaction also received 60 pmol of either the RED20-encoded or the standard-encoded expression vector ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1 μg of DNA was digested at 37C with the restriction enzyme EcoRI-HF (NEB) for two hours after which MspI (NEB ...
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bioRxiv - Cancer Biology 2019Quote: ... 1× protease inhibitor cocktail) and incubated with 12,000 gel units/ml micrococcal nuclease (NEB, M0247S) at 37 °C with frequently mixing to digest chromatin to lengths of approximately 100-to-1,000 bp ...
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bioRxiv - Biophysics 2019Quote: ... using a 5-fold excess of fluorophore to protein and ∼1 μM Sfp synthase (NEB) for 16 hours at 6 °C in 50 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Genetics 2019Quote: ... Using the 100 bp and 1 kb DNA Quick-Load ladders (N0467 and N0468, NEB) as a reference ...
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bioRxiv - Genetics 2021Quote: ... 1 μl of 10 mM dNTPs and 0.5 μl of OneTaq Polymerase (New England BioLabs). To generate (partially ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Q5 DNA Polymerase (NEB) with primer 8 & 9 or 10 & 11 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Taq DNA Polymerase (NEB) with primer 4 & 6 or 5 & 6 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Purified products were ligated with 1 µL T4 DNA ligase (protocol as per NEB®) for 10 minutes at room temperature (benchtop ...
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bioRxiv - Bioengineering 2021Quote: ... for 16 cycles annealing at 67 °C was mixed with 1 μL of ExoI (NEB) digested mixture as the template ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg standard glycoprotein was mixed in 1 μL of 10× glycoprotein denaturing buffer (NEB) (final conc ...