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Citations for New England Biolabs :
4351 - 4400 of 10000+ citations for Rat Heat Shock Protein Beta 2 HSPB2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids cleavage was conducted at 37 °C for 10 min and stopped by addition of 2×RNA loading dye (New England Biolabs, Ipswich, MA, USA). Before loading ...
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bioRxiv - Microbiology 2022Quote: CRISPR-Cas12a reactions were carried out in 20 μL reaction volumes with 2 μL NEBuffer™ r2.1 (New England Biolabs, Ipswich, Massachusetts, USA), 500 nM crRNA ...
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bioRxiv - Genomics 2022Quote: ... and the results (Figure 2) suggested that a combination of the rare cutter HindIII and the frequent cutter Sau3AI (New England Biolabs, NEB, Ipswich, MA) would provide restriction fragments suitable for ddRAD-Seq (150-700 bp).
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bioRxiv - Cell Biology 2024Quote: ... and treated for 10 min at RT by RNase III enzyme (2 U of RNase III (Short Cut, New England BioLabs, Massachusetts, USA, M0245S) with 20 mM MnCl2 in 1× reaction ShortCut buffer) ...
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bioRxiv - Cancer Biology 2024Quote: ... and chromatin was digested overnight by adding 25 µL 10X NEBuffer 2 and 100 U Mbo I (New England Biolabs, catalog No. R0147). Digestion efficiency was confirmed by isolating DNA from a 25-µL aliquot of the suspension and performing agarose gel electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... was amplified from JE2 using the primer pair 3810/9647 and the snap-tag coding sequence amplified from pSNAP-tag (T7)-2 (New England Biolabs, NEB; Supplementary Information) using the primer pair 9631/9632 ...
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bioRxiv - Biochemistry 2023Quote: ... In vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer (New England Biolabs Inc., Massachusetts, USA) with 1 μg RNA and 1 unit RNase Inhibitor (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... and produced by PCR amplification (10–13 cycles) of tagmented DNA using a NEB Next High-Fidelity 2× PCR Master Mix (New England Biolabs, Ipswich, MA, USA). DNA fragments were then purified using the MinElute PCR Purification Kit and eluted in 10 µL elution buffer ...
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bioRxiv - Plant Biology 2024Quote: ... The correct sequence was introduced into the destination vectors pCAMBIA1300-2× 35S [enhanced cauliflower mosaic virus (CaMV) 35S promoter] at the restriction enzyme sites BamHI and PstI (New England BioLabs, Ipswich, MA, USA). The sgRNAs were designed through CRISPR-P 2.0 (http://crispr.hzau.edu.cn/cgi-bin/CRISPR2/SCORE ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was collected and prepared using Monarch Total RNA Miniprep Kits (NEB). For SARS-CoV-2 experiments ...
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bioRxiv - Plant Biology 2020Quote: ... followed by subcloning into pEAQ-HT vector using the Gibson assembly kit (NEB). Linearization of pEAQ-HT vector was performed by XhoI and AgeI restriction enzyme double digestion ...
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bioRxiv - Plant Biology 2019Quote: ... The libraries were prepared using the Next Ultra RNA Library Prep Kit (NEB) by the company Novogene (China) ...
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bioRxiv - Microbiology 2020Quote: ... chloramphenicol or apramycin resistance cassette by using the Gibson isothermal assembly kit (NEB) followed by PCR amplification using the primers from the extremities ...
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bioRxiv - Microbiology 2020Quote: ... followed by vector-insert ligation using the Quick Ligation kit (New England Biolabs) to generate the original and mutated pCAGGS-HHRz-3M-eGFP-5M vectors ...
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bioRxiv - Cell Biology 2019Quote: ... rRNA was depleted using the NEBNext rRNA Depletion Kit (E6310, New England Biolabs) and subsequently directional RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420 ...
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bioRxiv - Immunology 2021Quote: ... The released DNA was cleaned up using Monarch DNA PCR Clean Kit (NEB) and eluted in TE buffer ...
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bioRxiv - Genetics 2021Quote: SpyCas9 tyr and tbxta sgRNAs were synthesized using EnGen sgRNA Synthesis Kit (NEB). SauCas9 ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplicon was purified using the Monarch PCR and DNA Cleanup Kit (NEB), and 1 ug used as template for transcription of digoxigenin labelled riboprobes using the DIG RNA Labelling mix (Sigma).
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bioRxiv - Genetics 2021Quote: ... The NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) was used for library preparation ...
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bioRxiv - Microbiology 2019Quote: ... and quantified using a NEBNext Library Quant kit for Illumina (New England BioLabs). Pooled libraries were then sequenced on a MiSeq platform (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... rad27 mutant alleles were constructed with the Q5 mutagenesis kit (New England Biolabs) using pEAA720 as template The oligonucleotides used to make the alleles are shown in Table S7) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA libraries were constructed using NEBNext DNA Library Prep Kits (New England Biolabs). Samples were processed and sequenced at Novogene (Hong Kong ...
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bioRxiv - Developmental Biology 2020Quote: cDNA was synthesized using the ProtoScriptII First Strand cDNA synthesis kit (NEB E6560S). Cloning of the full length admats9 from pooled cDNA from 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... They generated mRNA libraries with NEB RNA Ultra kit (New England Biolabs E7770L) and dToligos probe were used to target mRNA for cDNA synthesis ...
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bioRxiv - Bioengineering 2022Quote: ... Amplicons were then indexed using the NEBNext Multiplex Oligos for Illumina kit (NEB). Amplicons were then pooled and sequenced using a Miseq Nano with paired end 150 bp reads ...
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bioRxiv - Molecular Biology 2020Quote: ... by using NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520S).
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bioRxiv - Molecular Biology 2021Quote: ... The next day samples were purified using PCR cleanup kit columns (Monarch, NEB) and eluted using 50 μl kit elution buffer.
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were prepared using NEBnext Ultra DNA library prep kit for Illumina (NEB). Samples were paired-end sequenced at the iGE3 genomics platform of the University of Geneva.
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bioRxiv - Molecular Biology 2021Quote: ... Illumina libraries were constructed using the NEBNext Ultra II kit (New England Biolabs) and sequenced on an Illumina HiSeq2500 ...
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bioRxiv - Cell Biology 2022Quote: ... and adapter ligation were performed by using RNA library prep kit (E7530L, NEB). DNA products were cleaned using AMPure XP beads (Beckman) ...
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bioRxiv - Genomics 2022Quote: ... DNA was purified using a Monarch PCR & DNA Clean up kit (NEB, T1030) and eluted in 12 μL of water.
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bioRxiv - Genomics 2022Quote: The ligation reaction was carried out using Quick Ligation™ kit (NEB, M2200). A 10 μl mixture consist of 1 μl of 1 μM N40+22nt DNA (0.1 μM final) ...
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bioRxiv - Genomics 2020Quote: ... Libraries were generated using NEBnext Ultra DNA library preparation kit for Illumina (NEB). Libraries were sequenced by paired-end sequencing using a NextSeq 500 (Illumina).
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bioRxiv - Microbiology 2020Quote: ... the source plasmid (p-retronWT) was mutagenized through PCR using a kit (NEB; Q5-Site-Directed Mutagenesis Kit ...
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bioRxiv - Molecular Biology 2021Quote: All mutagenesis was done using Q5 Site-directed Mutagenesis Kit Protocol (NEB #E0554S) according to the manufacturer’s instructions using primers in table T1 ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... gel purifications were performed using the Monarch DNA Gel Extraction Kit (NEB T1020L), and minipreps were performed using the Monarch Plasmid Miniprep Kit (NEB T1010L) ...
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bioRxiv - Molecular Biology 2021Quote: ... NGS libraries were made using NEBNext Ultra II DNA Library Prep kit (NEB). DNA sequencing was performed on Illumina MiniSeq with 150+150 bp paired-end protocol.
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bioRxiv - Cancer Biology 2019Quote: ... Variants were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Plasmid transfection was performed with Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... and reverse-transcripted to cDNA using LunaScript RT SuperMix kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... PCR was done with the Phusion High-Fidelity PCR kit (New England BioLabs) with 40ng of gDNA ...
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bioRxiv - Plant Biology 2019Quote: TNIC317S active site mutant was generated using Q5 Site-Directed Mutagenesis Kit (NEB).pGEM-T-TNI served as a template for making the site directed mutant using primers P2346 and P2347 ...
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bioRxiv - Genomics 2019Quote: ... the DNA was treated with repair enzymes (New England Biolabs PreCR kit M0309) and purified using Qiagen PCR purification columns as above.
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bioRxiv - Microbiology 2019Quote: cDNA synthesis reactions were carried out using the Lunascript RT Supermix kit (NEB) and 500 ng of RNA was used for each reaction.
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bioRxiv - Evolutionary Biology 2019Quote: ... CPS mutant plasmids were constructed using the Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Cancer Biology 2019Quote: ... SPA4CT-T43P was generated using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) using pLVX SPA4CT as a template ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA samples were depleted of rRNA using the NEBNext rRNA Depletion kit (NEB). RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep kit (NEB) ...
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bioRxiv - Synthetic Biology 2019Quote: ... For cloning purposes DNA was purified using Monarch PCR & DNA Cleanup Kit (NEB) and assembled using NEBuilder HiFi DNA Assembly Cloning Kit (NEB) ...
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s recommendations (Magnetic mRNA Isolation Kit protocol from NEB, S1550S), and eluted in 17 μL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7765) and dual indexing from the NEBNext Multiplex Oligos kit (NEB #7600 ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNA was captured using NEBNext (Poly A) mRNA magnetic isolation kit (NEB, E7490) according to manufacturer’s instructions ...