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Citations for New England Biolabs :
4251 - 4300 of 10000+ citations for Rat Heat Shock Protein Beta 2 HSPB2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: All plasmids were constructed using Hifi DNA Assembly Cloning Kit (NEB, USA) and then midipreped with ZymoPure Midiprep Kit (Zymo Research ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The RNA samples were cleaned with Monarch RNA Cleanup Kit (NEB #T2030) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmid DNA was extracted using the Monarch Miniprep Kit (NEB, Cat. #T1010L) and sent to sequencing to identify mutations.
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bioRxiv - Developmental Biology 2024Quote: ... using the NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA).
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: Total RNA was extracted with Monarch Total RNA Miniprep Kit (NEB #T2010S). The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the Monarch PCR Cleanup Kit (NEB, T1030L) and digested with the TfiI restriction enzyme (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA).
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bioRxiv - Developmental Biology 2024Quote: ... using the NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA) and recombined into a UASp-attB destination vector (gift of F ...
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bioRxiv - Microbiology 2024Quote: ... The Primer-free LunaScript® RT Master Mix Kit (New England Biolabs) was used for first strand cDNA synthesis using a gene-specific primer targeting the mCherry gene in the genomic vRNA (primer sequence 5’-CGCTTCAAGGTGCACATGG-3’) ...
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bioRxiv - Cell Biology 2024Quote: ... The insertion was done using the Q5 Site-Directed Mutagenesis Kit (NEB) and design of respective primers using the NEBaseChanger online tool (http://nebasechanger.neb.com/).
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bioRxiv - Cell Biology 2024Quote: ... and plasmids were purified using a Monarch Plasmid Miniprep Kit (NEB #T1010). Successful mutagenesis was verified through DNA sequencing (Plasmidsaurus).
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bioRxiv - Cell Biology 2024Quote: ... and plasmids were purified using a Monarch Plasmid Miniprep Kit (NEB #T1010). Constructs were then verified by restriction digest using SacI-HF (NEB #R3156 ...
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bioRxiv - Cell Biology 2024Quote: ... The RNA was then made using a T7 Highscribe kit (NEB #E2040S), treated with DNase I (NEB #M0303L ...
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bioRxiv - Microbiology 2024Quote: ... using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). Homology-directed repair templates were PCR amplified using the CloneAmp™ HiFi PCR Premix (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... and HiFi DNA Assembly kit were purchased from New England Biolabs (NEB). All bacterial strains were PCR or sequence verified before use ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was extracted using the Monarch gDNA Purification Kit (NEB T3010S) following the standard protocol but with elution using 175 µL 25 mM K3BO3 at pH 7.0.
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared with the NEBNext Ultra II Directional RNA Kit (NEB). Samples were equimolarly pooled and sequenced on a NextSeq550 system (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... PCR-amplified DNA fragments were assembled by Gibson assembly kit (NEBuilder, NEB) as per manufacturer’s protocol and introduced into DH5α E ...
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bioRxiv - Cell Biology 2024Quote: ... we use the Monarch® Genomic DNA Purification Kit (New England Biolabs) and follow the manufacturer recommendation ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were additionally processed with the Monarch RNA Cleanup Kit (NEB), analyzed for integrity with a TapeStation (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: Monarch® Genomic DNA Purification Kit (New England Biolabs, cat. no. T3010S)
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bioRxiv - Cell Biology 2024Quote: Monarch® PCR & DNA Cleanup Kit (New England Biolabs, cat. no. T1030S)
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to manufacturer’s directions ...
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bioRxiv - Molecular Biology 2024Quote: ... In-vitro transcription (HiScribe T7 High Yield RNA Synthesis Kit E2040L, NEB) from the DNA was then carried out ...
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bioRxiv - Molecular Biology 2024Quote: ... rRNA depletion was conducted using NEBNext rRNA depletion kit v2 (NEB, #E7400L) followed by end-repair using T4 PNK after purification of the rRNA depleted samples using Oligo Clean & Concentrator Kits (Zymo research ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using LunaScript RT SuperMix Kit (New England Biolabs, USA). SYBR green assay was used to determine HBV pregenomic expression (Luna Universal qPCR Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... Then we used ProtoScript® II First Strand cDNA Synthesis Kit (NEB) and 3′-RACE CDS primer (ClonTech ...
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bioRxiv - Molecular Biology 2024Quote: ... then purified using the Monarch RNA Cleanup Kit (T2040L, New England Biolabs). Quality and size of the produced RNAs were assessed using an Agilent Fragment Analyzer.
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed with the LunaScript RT SuperMix Kit (#E3010, NEB) using the manufacturer’s protocol on a SimpliAmp thermal cycler (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were recovered using Monarch Plasmid Miniprep Kit (New England Biolabs #T1010S) or E.Z.N.A ...
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bioRxiv - Microbiology 2024Quote: ... Digested products were cleaned using Monarch® PCR & DNA Cleanup Kit (NEB) and incubated with T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and cleaned with the Monarch RNA Cleanup Kit (NEB, Ipswich, MA, USA). Ribosomal 23S and 16S samples were prepared by amplifying the appropriate sequences from genomic E ...
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bioRxiv - Microbiology 2024Quote: Total RNA samples were treated with a NEBNext rRNA depletion kit (NEB) to remove rRNA ...
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bioRxiv - Biochemistry 2024Quote: ... Transcription was performed using T7 HiScribe RNA synthesis kit (New England Biolabs). Generated RNA was subjected to DNase treatment (TURBODNase ...
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bioRxiv - Cancer Biology 2024Quote: ... using the NEBNext® Ultra TM II DNA Library Prep Kit (NEB E7645S/L ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified with the Monarch Total RNA Miniprep Kit (New England Biolabs). Alkylated RNA was purified with the Monarch RNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the Monarch PCR Cleanup Kit (NEB, T1030L). PCR products were A-tailed ...
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bioRxiv - Genetics 2020Quote: For Southern blot analysis 150 ng of mouse DNA was digested with 10 units of BamHI-HF (NEB, Figure 2 and 5), NdeI (NEB ...
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bioRxiv - Physiology 2020Quote: ... pNP152 was obtained by insertion of the sta-2 promoter and sta-2 gene fused to Lifeact::mKate2 [75] into the MosSCI vector pCFJ151 [76] using Gibson Assembly (NEB Inc., MA) and confirmed by PCR or sequencing ...
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bioRxiv - Molecular Biology 2019Quote: ... novel donor template for AAV) were assembled in a ratio of 1:2 with Gibson (HiFi) DNA Assembly Master Mix (NEB, cat# E2621S) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The probes were prepared by end labelling 10 pmoles of DNA oligonucleotide complementary to the sfRNA (Supplementary Table 2) with [γ-32P]-ATP (Perkin-Elmer, USA) using T4 polynucleotide kinase (NEB, USA) and purified from unincorporated nucleotides by gel filtration on Illustra MicroSpin G-25 Columns (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Biotinylated nucleotides from the non-ligated DNA ends were removed by incubating the Hi-C libraries (2 μg) in the presence of 6 U of T4 DNA polymerase (NEB; Cat#: M0203L) in NEBuffer 2.1 supplied with 0.025 mM dATP (Thermo Fisher ...
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bioRxiv - Plant Biology 2021Quote: ... nigrum young leaf and peduncle cDNA using c70979_Fw and c70979_Rv primer and cloned into the pEAQ-HT vector(50) using NruI-HF and SmaI restriction sites and 2× Gibson Assembly master mix (NEB, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... for 2 hr at 60 °C and relinearized at the basic dSpacer furan with Ape 1 (15 U; M0282S, NEB, Ipswich, MA) for 2 hr at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The relinearized single-stranded DNA template was subjected to PCR amplification by using barcoded primers for Illumina TrueSeq small RNA sample and Phusion High-Fidelity DNA Polymerase (2 U; M0530L, NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs were subcloned into vectors through conventional ligation with Ligation high Ver.2 (Toyobo, Osaka, Japan) or NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA) according to the manufacturers’ instruction ...
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bioRxiv - Synthetic Biology 2022Quote: ... Single primer PCR was performed in 2 different tubes for each plasmid using Q5 2X Master Mix (New England Biolabs Cat.No M0492S). After the amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... µM GAT-7N primers (5′- GTG AGT GAT GGT TGA GGT AGT GTG GAG NNN NNN N) and 2 units/µl DeepVent® (exo-) DNA polymerase (New England Biolabs, M0259L) in the programmable thermal cycler for 11 cycles ...
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bioRxiv - Microbiology 2021Quote: ... The construct was amplified by 2-step PCR according to manufacturer recommendations using Q5 high fidelity polymerase (New England Biolabs, cat#M0494S) with primers 6469TSC and 6470TSC (Supplemental table 1) ...
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bioRxiv - Microbiology 2021Quote: ... Triplicate NEBuilder HiFi assembly reactions were prepared according to manufacturer’s protocols containing ∼2:1 insert to vector as follows: 10 µl of 2X NEBuilder HiFi master mix (New England Biolabs, cat#E2621S), 158.7 ng of DNA fragments ...