Labshake search
Citations for New England Biolabs :
4351 - 4400 of 9611 citations for Anti DBH SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... The kinase-dead MELKT167A mutant was constructed using a Q5 site-directed mutagenesis kit (New England Biolabs) with 5′-aggattaccatctacaggcatgctgtgggagtctg-3′ forward and 5′-cagactcccacagcatgcctgtagatggtaatcct-3′ reverse primers.
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bioRxiv - Genetics 2021Quote: ... the fragments were analyzed by gel electrophoresis and purified using a Monarch DNA Gel Extraction Kit (NEB). All strains had the expected variants in the sequenced fragment ...
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bioRxiv - Genomics 2020Quote: ... We extracted RNA using Monarch® Total RNA Miniprep Kit (New England BioLabs GmbH, Frankfurt, D, E2010). Material was ground in Mixer Mill 200 (Retsch GmbH ...
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bioRxiv - Genomics 2020Quote: ... we converted purified mRNA to 300 bp fragment DNA libraries with the Ultra II Directional kit (NEB) and indexed them for multiplexing (NEB).
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from cells with the Monarch Total RNA Miniprep Kit (New England BioLabs, #T2010S) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... This plasmid was then digested with BamHI and NotI and the resulting insert (5’esaAflank_SpecPromoter_kanR_3’esaAflank) was gel extracted (Monarch DNA Gel Extraction Kit, NEB). 100 ng of purified insert was transformed into S ...
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bioRxiv - Microbiology 2020Quote: ... total isolated RNA was reverse transcribed using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB), according to the manufacturer’s instructions and using a random primer mix (Promega) ...
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bioRxiv - Plant Biology 2021Quote: ... The complementary DNA (cDNA) was synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) following the manufacturer’s instructions with primer d(T)23 VN ...
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bioRxiv - Microbiology 2021Quote: ... or built directly into the plasmid of interest using the NEBuilder HiFi Assembly kit (New England Biolabs), transformed into E ...
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bioRxiv - Microbiology 2021Quote: ... crRNA was synthesized in vitro using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) by incubating the reaction at 37°C with T7 promoter primer (10μM ...
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bioRxiv - Molecular Biology 2021Quote: ... all ChIP-DNA libraries were produced using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645L) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and in vitro synthesized RNA was subsequently purified using Monarch RNA Cleanup Kit (Cat. No. T2040S, NEB). RNA concentration was quantified by NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... followed by library preparation with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Library preparation included 11 cycles of PCR amplification ...
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bioRxiv - Microbiology 2021Quote: ... SERBP1 mutant and Vigilin mutant were obtained using the Q5® Site-Directed Mutagenesis Kit (E0554) (NEB) with deletion primers using the WT cDNA in pLVX as template ...
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bioRxiv - Immunology 2020Quote: ... (2) and (3) was synthesized through HiScribe™ T7 ARCA mRNA Kit (with tailing) (New England Biolabs). All the mRNA products were concentrated and purified via EZ-10 Spin Column RNA Cleanup & Concentration Kit (Bio Basic Inc.) ...
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bioRxiv - Microbiology 2021Quote: ... were Deletions and point mutations in HelR were created using the Q5 site directed mutagenesis kit (NEB). Primers for mutant construction were designed using the NEBaseChanger as recommended ...
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bioRxiv - Microbiology 2021Quote: ... The DNA samples were pooled into two libraries prepared with the NEBNext Ultra kit (New England BioLabs). They were sequenced on a MiSeq sequencer (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments were amplified by PCR and assembled by NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S).
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bioRxiv - Genetics 2020Quote: ... RNA was reverse transcribed to cDNA using the ProtoScript II First Strand synthesis kit (NEB, Cat# E6560S) and synthesis was performed with the “random primer” option included within the kit ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genomics 2021Quote: ... we purified the genomic DNA from each timepoint with the Monarch gDNA extraction Kit (New England Biolabs). We then amplified the region of one of the mutations acquired by the Tf-null strain (I:1453108 C->A ...
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bioRxiv - Genomics 2021Quote: ... gDNA was extracted with the Monarch Genomic DNA purification kit (#T3010 New England Biolabs; Ipswich, MA, USA). For Oxford Nanopore sequencing of STK005 ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were produced using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on Illumina HiSeq platform.
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bioRxiv - Genomics 2021Quote: ... Standard RNA Synthesis was carried out with the HiScribe T7 High Yield RNA Synthesis kit (NEB E2040S) for 1.5 hours according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Libraries were prepared for high-throughput sequencing using the Ultra II DNA Library Prep kit (NEB E7645L). For IP samples ...
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bioRxiv - Genomics 2020Quote: ... Plasmid libraries were harvested from liquid culture using the Monarch® Plasmid DNA Miniprep Kit (NEB #T1010). Miniprep kit reagents were scaled to accommodate one spin column for every 5mL of culture.
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bioRxiv - Microbiology 2021Quote: ... Strand-specific RNA-seq was performed using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, E7420) with the following adjustments ...
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bioRxiv - Biochemistry 2020Quote: ... TSEN2H377A and TSEN34H255A point mutants were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) prior to assembly into biGBac vectors ...
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bioRxiv - Cancer Biology 2021Quote: Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations with index codes added to attribute sequences to each sample ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We prepared cDNA libraries with the NEBNext Ultra RNA Library Prep Kit for Illumina (NewEngland Biolabs, MA) with poly(A ...
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bioRxiv - Molecular Biology 2021Quote: ... Non-binding mutant (F212A) was generated from template MHV-S_HE_Fc_Avitag using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs)
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bioRxiv - Microbiology 2022Quote: ... DNAse treatment was performed as described above and cDNA was synthesized using LunaScript RT Supermix Kit (NEB). RT and non-RT vials were checked by PCR with primers 7,8 for PgV (Supplementary File 2) ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 µL of dNTPs (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, NEB, Ipswich, MA, USA), 0.5 µL of T7 polymerase (HiScribe™ T7 Quick High Yield RNA Synthesis Kit ...
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bioRxiv - Developmental Biology 2022Quote: ... end prep and ligation) was performed using the ‘NEB Ultra II RNA custom kit’ (New England Biolabs) on an Agilent Bravo WS automation system ...
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bioRxiv - Genetics 2022Quote: ... poly-A RNA libraries were generated using (NEBNext Ultra Directional RNA Library Prep kit, New England Biolabs, Ipswich ...
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bioRxiv - Cell Biology 2022Quote: ... Mutants of IR and IGF1R were generated by Q5 site-directed mutagenesis kit (New England BioLabs Inc.). Plasmid transfections into 293FT cells were performed with LipofectaminTM 2000 (Invitrogen) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The two PCR fragments were then assembled using the HiFi Gibson Assembly kit from NEB (Waltham, MA) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... Non-fluorescent RNA was transcribed using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, #E2040S). Following in vitro transcription by either kit ...
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bioRxiv - Molecular Biology 2022Quote: ... Indexed sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep kit (NEB Cat # E7645), pooled ...
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bioRxiv - Molecular Biology 2022Quote: DNA libraries for deep sequencing were made using the NEBNext Ultra II DNA library preparation kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... fragmented DNA was processed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Library preparation was modified from protocols.IO (dx.doi.org/10.17504/protocols.io.bagaibse) utilizing reagents from the NEB Next II DNA Ultra Kit (New England Biolabs #E7645S) and Unique Combinatorial Dual index kit (New England Biolabs #E6442S, ...
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bioRxiv - Biophysics 2022Quote: ... sgRNA was produced by in vitro transcription using the HiScribe T7 High Yield RNA synthesis kit (NEB) with the PCR amplified DNA sequence as the template ...
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bioRxiv - Biochemistry 2022Quote: ... products corresponding to the correct size were excised and purified using Monarch DNA Gel Extraction kit (NEB). For the second round of PCR ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products corresponding to the correct size were excised and purified using DNA Gel Extraction kit (NEB). 100 ng of each gel-purified PCR products (total of 19 ...
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bioRxiv - Biochemistry 2022Quote: The amplicons described above were purified with the Monarch PCR and DNA purification kit (New England Biolabs) and sent for NGS EZ Amplicon Sequencing by Genewiz ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Biochemistry 2022Quote: ... Polyadenylated transcript enrichment and strand specific library preparation was completed using NEBNext Ultra II mRNA kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was purified by standard methods and rRNA depleted using the NEBNext rRNA depletion kit (NEB, as per the manufacturer’s instructions) ...
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bioRxiv - Developmental Biology 2022Quote: ... the library was prepared using the NEBNext Ultra RNA library prep kit for Illumina (New England Biolabs). Sequences with 150 bp read length × 2 paired ends and 6 Gb of data were obtained using the next-generation sequencer ...