Labshake search
Citations for New England Biolabs :
4601 - 4650 of 9611 citations for Anti DBH SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were made with the NEBNext Ultra II DNA Library Prep kit (New England Biolabs E7645L) and cleaned-up using SPRIselect beads (Beck-man Coulter B23317) ...
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bioRxiv - Neuroscience 2023Quote: 150 ng of total RNA was applied to the NEBNext rRNA Depletion Kit (New England Biolabs, #E6310L) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Single-end strand-specific libraries were prepared using NEBNext Ultra RNA library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and converted this mRNA to 280-300 bp cDNA fragments using the Ultra II Directional Kit (NEB). Unique sequencing adapters were added to each cDNA library for multiplexing (NEB).
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bioRxiv - Plant Biology 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using NEBNext Ultra II library preparation kits for Illumina (New England BioLabs, Ipswich, MA). Each pool was indexed with a unique NEBNext i7 adapter and an i5 adapter containing a degenerate barcode and PCR amplified with 12 cycles ...
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bioRxiv - Microbiology 2023Quote: ... Eluted RNA was depleted from ribosomal RNA using NEBNext rRNA Depletion Kit (Bacteria, New England Biolabs, MA) then used as template to generate cDNA using NEBNext Ultra RNA Library Prep Kit for Illumina (England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA-seq libraries were generated using the Next Ultra RNA Library Prep Kit for Illumina (NEB) as described before (Berneking et al ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from cell lysates using the Monarch Total RNA Miniprep Kit (T2010S, New England BioLabs) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... The sgRNAs were prepared using the EnGen sgRNA synthesis kit following the manufacturer’s protocol (New England BioLabs), and then associated with EnGen Spy Cas9 NLS protein (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... was constructed by assembling 3 DNA fragments using the NEBuilder HiFi DNA assembly kit (New England Biolabs). All 3 fragments were amplified from pTRL2-His-GrgA 36 using Q5 DNA polymerase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Libraries were prepared using the NEB Next® UltraTM DNA Library Prep Kit (New England Biolabs, U.S.A) with a target insert size of 350 nt and six PCR cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... Tm or EPEC FliCD0 constructs were generated with the Q5 site-directed mutagenesis kit (New England Biolabs) following manufacturer recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: In vitro translation was carried out with a custom PURExpress ΔRF-123 ΔRibosome kit (New England Biolabs), using ∼5 pmol of 5’-biotinylated and 3’-polyadenylated mRNA as a template ...
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bioRxiv - Microbiology 2023Quote: ... The metagenomic library was constructed using NEBNext® Ultra DNA Library Prep Kit for Illumina (NEB, USA). The samples were sequenced with the PE150 strategy on Illumina Hiseq Novaseq 6000 platform (commercial service ...
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bioRxiv - Molecular Biology 2023Quote: RT-qPCR experiments were performed with the Luna® Universal One-Step RT-qPCR Kit (NEB, #E3005E) with a final reaction volume of 10 μL per well ...
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bioRxiv - Molecular Biology 2023Quote: ... the HiScribe® T7 High Yield RNA Synthesis Kit from New England Biolabs (NEB E2040S, Ipswich, MA) was used with the standard RNA synthesis protocol and incubated for 4 hours ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and lysed using the Tissue Lysis buffer from the Monarch® Genomic DNA Purification Kit (NEB, #T3010S) with double the volume of lysis buffer and a 1 h incubation at 56 C ...
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bioRxiv - Neuroscience 2023Quote: ... and Cttn-VC155 were generated by NEBuilder® HiFi DNA Assembly Cloning Kit (# E5520S, New England BioLabs), which enables the assembly of multiple inserts within a linearized vector in a one-step PCR reaction ...
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bioRxiv - Neuroscience 2023Quote: ... After quantification of individual libraries by q-PCR using the NEBNext library quant kit (New England BioLabs), all the samples were pooled in an equimolar manner ...
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bioRxiv - Microbiology 2023Quote: We performed RT-qPCR on select genes using the Luna Universal One-Step Kit (New England Biolabs) and QuantStudio 5 (Thermo) ...
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bioRxiv - Genetics 2024Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified pools were subjected to PCR product purification by Monarch PCR and DNA Cleanup Kit (NEB). The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).
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bioRxiv - Cancer Biology 2024Quote: ... GAPF libraries were constructed with the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs), which employs fragmentase for DNA fragmentation ...
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bioRxiv - Cancer Biology 2024Quote: ... and poly(A) mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation kit (NEB E7490L). The RNA library for direct RNA sequencing was prepared from ∼500 ng of poly-A RNA according to the Oxford Nanopore technology (ONT ...
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bioRxiv - Synthetic Biology 2024Quote: ... The variants were created from the wild type plasmids using the Q5 Site-Directed Mutagenesis Kit (NEB) and transformed into MegaX cells made chemically competent (Mix & Go E ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were submitted to Novogene for library preparation (NEB Next® Ultra™ RNA Library Prep Kit) and paired-end (150bp ...
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bioRxiv - Neuroscience 2024Quote: ... and cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560L). cDNA was amplified using primer pairs designed to either target two wildtype sequences on either side of the cryptic exon or one wildtype sequence and another sequence directly in the cryptic exon ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from samples using the Monarch® Total RNA Miniprep Kit (New England Biolabs, #T2010S) and cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted using the Monarch genomic DNA extraction and purification kit (New England Biolabs Inc.). Region of Cori and ter were quantitatively amplified using the Sybr green-based Luna Universal One-step RT-qPCR kit (New England Biolabs Inc.) ...
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bioRxiv - Microbiology 2023Quote: ... ChIP-sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) and sequenced with an Illumina HiSeq2500 (50 bp single-end) ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA was quantified using a Qubit 4 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The library was quantified using the NEBNext® Library Quant Kit for Illumina® (New England Biolabs) by mixing 2 ul 1:10.000 diluted library with 8 ul Quant mastermix with added primers (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... Colonies were prepared for whole genome sequencing using an Epicentre Masterpure DNA Preparation Kit (Nordic Biolabs, Sweden). Genomic DNA libraries were constructed using Nextera XT Library Preparation Kit (Illumina ...
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bioRxiv - Immunology 2023Quote: ... IVT was done using the HiScribe® T7 High-Yield RNA Synthesis Kit (New England Biolabs, #E2040S), with substitution of N-1-methyl-pseudouridine-5’-triphosphate (TriLink Biotechnologies ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: Total RNA extraction was performed using NEB Monarch Total RNA Miniprep Kit (New England Biolabs Cat #T2010S). RNAseq library prep was performed using NEBNext Ultra II RNA Library Prep for Illumina (NEB Cat# E7770S) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA library was prepared using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs, E7645) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, Cat #E7645) following manufacture’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was constructed using an NEBNext rRNA Depeletion Kit (Bacteria) (New England Biolabs, Ipswich, MA). From the kit ...
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bioRxiv - Microbiology 2023Quote: ... which was transcribed in vitro using the HiScribe T7 high-yield RNA synthesis kit (New England Biolabs). Afterwards ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting gRNAs were purified using the AMPure XP kit and treated with DNase I (NEB, UK). Concentration was evaluated on a Qubit system (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA seq libraries were constructed from total RNA using the NEBNext Ultra II Directional RNAseq kit (NEB) with ribosomal RNA depletion ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently prepared for RNA-seq with the NEBNext Ultra Directional RNA Library Prep Kit (NEB #E7420) at GenomeScan ...
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bioRxiv - Microbiology 2023Quote: cDNA was synthesized from extracted viral RNA using the LunaScript® RT SuperMix Kit (NEB, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... 4NBX.B WT sequence with C103A mutation was created by site-directed mutagenesis using commercially available kits (NEB). Smt3 SUMO protein with an N-terminal cysteine was created from wild type Smt3 SUMO by mutagenic PCR ...
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bioRxiv - Cell Biology 2023Quote: ... Exon library was constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB). DNA library hybridization was performed by using Agilent SureSelect Human All Exon V6 (Agilent) ...
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bioRxiv - Cancer Biology 2023Quote: ... NGS libraries were prepared using NEBnext Ultra II DNA library preparation kit for Illumina (New England Biolabs) and quantified with using a TapeStation (Agilent Genomics) ...
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bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Strand-specific RNA libraries were generated using the NEBNext Ultra II stranded library kit (New England Biolabs) combined with polyA-coupled beads (New England Biolabs ...