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Citations for New England Biolabs :
4301 - 4350 of 9843 citations for Swine IL 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of libraries was determined using NEBNext library Quant kit for Illumina (NEB, Cat# E7630). Libraries were then pooled and sequenced by Macrogen on Ilumina Hiseq X-ten platform ...
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bioRxiv - Molecular Biology 2023Quote: crRNA (Supplementary Table 2) was synthesized using a HiScribe T7 High Yield RNA Synthesis Kit (NEB). The DNA sequences includes the T7 promoter at the 5’ end and the sequence from crRNA with the target sequence at the 3’ end ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing libraries were prepared with NEBNext rRNA Depletion Kit v2 (New England Biolabs, Ipswich, MA) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of purified DNA template was used in the Hi-Scribe T7 transcription kit (NEB) at 37°C overnight (∼16 hr.) ...
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bioRxiv - Genomics 2024Quote: ... and final library amplification were performed using the NEBNext Enzymatic Methyl-seq Kit (New England BioLabs) for WGEM-seq libraries ...
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Evolutionary Adaptation of an HP1-protein Chromodomain Integrates Chromatin and DNA Sequence SignalsbioRxiv - Genetics 2024Quote: ... Barcoded libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to manufacturer’s instructions and sequenced on a NovaSeqSP instrument (Illumina).
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bioRxiv - Cancer Biology 2024Quote: ... All single-cell libraries were prepared using the NEBNext Ultra II FS kit (New England Biolabs) following the manufacturer’s instructions but using one-fourth volumes ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E7645) following manufacture’s protocol ...
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bioRxiv - Genetics 2024Quote: ... A NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) was used for library preparation ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative RT-PCR was conducted with the Luna Universal One-Step RT-qPCR Kit (NEB, E3005) on a Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs) and then ligated to the 3’ end of the cDNA by using Thermostable 5’ App DNA/RNA Ligase (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB, E7760S, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645L) and single-end (1 × 75 bp ...
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bioRxiv - Cancer Biology 2024Quote: ... the DNA fragments were purified from gel electrophoresis using the Monarch DNA gel extraction kit (NEB), fragments and destination plasmid were digested using the respective restriction enzymes (NEB ...
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bioRxiv - Genetics 2024Quote: ... Up to 1µg was the used for reverse transcription using the ProtoScript Reverse Transcription kit (NEB). qPCR was done using iTaq universal SYBR Green Supermix (BioRad ...
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bioRxiv - Immunology 2024Quote: ... In vitro transcription was then done with the HiScribe T7 High Yield RNA Synthesis Kit (NEB), template DNA was removed with DNase I (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were produced with a NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on an Illumina NovaSeq 6000 platform using a 2 × 150 bp run protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cleaned up A-tailed DNA went through Adaptor ligation reaction using Quick Ligation Kit (NEB, M2200L) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using the NEBNext Ultra RNA libray Prep Kit for Illumina (NEB, USA). RNA sequencing was performed using the Illumina NovaSeq platform ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using the Luna script RT Supermix kit (New England Biolabs, Cat#M3010L). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted DNA was enriched by PCR using Phusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... was used for library preparation of total RNAs and Next small RNA Kit (New England Biolabs) for library preparation of microRNAs according to manufacturerś instructions ...
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bioRxiv - Microbiology 2024Quote: A NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, cat. no. E7645S) protocol for Illumina was used to prepare DNA libraries for sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, E2040S) and purified using Monarch® RNA Cleanup Kit (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... and libraries for Illumina sequencing were performed using the NEBNext Ultra II RNA kit (NEB, #E7770L). Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes ...
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bioRxiv - Microbiology 2024Quote: ... UDI-barcoded RNAseq libraries were generated with the NEBNext Ultra II RNA Library Prep Kit (NEB). Each library was quantified with a Qubit (dsDNA HS kit ...
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bioRxiv - Immunology 2024Quote: ... Further modifications were performed by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB). All modifications to the 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO plasmid (Addgene ...
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High-resolution chromosome-level genome provides molecular insights into adaptive evolution in crabsbioRxiv - Genomics 2024Quote: ... Sequencing libraries were constructed using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA), following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was then purified from the quenched reaction mixtures using Monarch® RNA Cleanup Kit (NEB) according to manufacturer protocols and subsequently quantified by NanoDrop.
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bioRxiv - Bioengineering 2024Quote: ... and cDNA libraries were generated by RT-PCR using the ProtoScript II cDNA Synthesis Kit (BioLabs) according to the manufacturers’ protocols ...
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bioRxiv - Zoology 2024Quote: ... RT-qPCR was performed using the Luna One-step RT-qPCR kit (New England Biolabs, Germany) on a 384 well-plate with 3 technical replicates per biological replicate in CFX384 Touch Real-Time PCR (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: ... Sequencing adapters were ligated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) while DNA was bound to the beads ...
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bioRxiv - Genomics 2024Quote: ... The recovered DNA was subjected to library construction using NEBNext Ultra DNA library prep kit (NEB). The sequencing libraries were amplified by PCR using the NEBNext Ultra II Q5 Master Mix (NEB ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Cell Biology 2024Quote: ... and reversely transcribed into cDNA using the ProtoScript II NEB cDNA synthesis kit (New England Biolabs).
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bioRxiv - Developmental Biology 2024Quote: ... plasmid templates were linearized with NotI and purified using Monarch PCR purification kits (New England Biolabs). The purified templates were transcribed using mMESSAGE mMACHINE Sp6 (Thermo-Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... DNA was subsequently purified with a Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Val206 of Envy was mutagenized to Lys with the Q5 Site-Directed Mutagenesis Kit (NEB #E0554S) using primers listed in Table S2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... A NEB Monarch® Plasmid Miniprep Kit was used for RT-DNA extraction (New England Biolabs). Following miniprep ...
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bioRxiv - Evolutionary Biology 2024Quote: ... After the reaction we purified the PCR product with Monarch PCR and DNA Cleanup Kit (NEB).
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bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR product was cloned into the pCDF4 vector using the Gibson Assembly kit (NEB) and the resulting plasmid was then used to generate ATG4-gRNA transgenic flies via injection into the y1 ...
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bioRxiv - Immunology 2024Quote: ... and subsequently replaced with the tailed rpoS DNA fragment using the HiFi DNA assembly kit (NEB). Following the assembly reaction ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesised according to the protocols of the LunaScript RT Supermix Kit (New England Biolabs) and for qPCR amplification ...
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bioRxiv - Plant Biology 2020Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB), following the manufacturer’s recommendations and sequenced on Illumina Hiseq platform and 150 bp paired-end reads were generated.
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bioRxiv - Microbiology 2020Quote: ... and ftsH target sequences was carried out in vitro using a Q5 mutagenesis kit (New England BioLabs) with specified primers (S1 Table) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was collected at 9 h post-IFN treatment using Monarch Total RNA Miniprep Kits (NEB). RNA was prepped for RNA sequencing submission using the NEBNext Poly(A ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR reactions were purified with Monarch DNA Cleanup Kit columns (T1030; New England Biolabs; Ipswich, MA, USA) per the manufacturer’s protocol and eluted in 20 µL nuclease-free water.
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bioRxiv - Genomics 2021Quote: ... 1μl RT Adapter (110nM; ONT Kit) and 1.5μl T4 DNA Ligase (2M U/ml New England BioLabs). The ligation reaction was carried out for 10min at RT ...