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Citations for New England Biolabs :
4251 - 4300 of 9843 citations for Swine IL 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was derived from total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S). Numerous primers were designed against cryptic exon targets and screened to identify primer pairs that minimized background bands.
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bioRxiv - Molecular Biology 2023Quote: ... The samples were prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Quantitative RT-PCR was performed with an Mx3000P qPCR system (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... va mtSSB ΔMTS by PCR amplification and ligation (Q5 Site-Directed Mutagenesis Kit, NEB, Cat #E0554). The plasmid insert was sequence-verified and then transformed into E ...
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bioRxiv - Neuroscience 2023Quote: ... The libraries were quantified using the NEBNext Library Quant Kit for Illumina (#E7630; New England Biolabs) based on the mean insert size provided by the Bioanalyzer ...
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bioRxiv - Neuroscience 2023Quote: ... We quantified the libraries using the NEBNext Library Quant Kit for Illumina (New England Biolabs, #E7630) based on the mean insert size provided by the Bioanalyzer ...
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bioRxiv - Bioengineering 2023Quote: ... NEBNext Ultra II Directional RNA Library Prep Kit (Catalog # E7760, NEW ENGLAND BIOLABS,MASSACHUSETTS, UNITED STATES), NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Cell Biology 2023Quote: ... FlucWT-HA-GFP11 and FlucDM-HA-GFP11 plasmids were constructed using site-directed mutagenesis kit (NEB) based on FlucSM-HA-GFP11 ...
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bioRxiv - Cancer Biology 2023Quote: ... the NEBNext Ultra II DNA Library Prep Kit for Illumina was followed (NEB, cat. no. E7645S) using total DNA yield as input material ...
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bioRxiv - Physiology 2023Quote: ... Samples were reversed transcribed using the LunaScript RT SuperMix Kit (New England BioLabs, Ipswich, Massachusetts, USA) and relative gene expression was quantified via CFX-96 Real-Time PCR Detection System (RRID:SCR_018064 ...
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bioRxiv - Synthetic Biology 2024Quote: ... library concentrations were quantified with qPCR using the NEBNext Library Quant Kit for Illumina (NEB, E7630S), and pre- and post-selection libraries were combined as two pools ...
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bioRxiv - Plant Biology 2024Quote: ... The sgRNA was first synthesised using the EnGen sgRNA synthesis kit New England Biolabs (NEB #E3322) before mixing with the Cas9-NLS to form the RNP complex ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, #E7420L), followed by sequencing on an Illumina Hiseq 4000 system ...
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bioRxiv - Genetics 2024Quote: ... The resulting product was inserted into linearized pKR482 using NEBuilder HiFi DNA assembly kit (NEB E5520S) and transformed into 10-beta cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... IVT-produced RNA samples were purified using a Monarch® RNA Cleanup Kit (New England Biolabs). ssRNA during RNA extractions was degraded with RNase T1 (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: 7.5ug of total RNA was immunoprecipitated for m6A with EpiMark® N6-Methyladenosine Enrichment Kit (NEB) following the manufacture protocol with 10% of the sample RNA taken as input prior to the pulldown ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... cDNA was synthesized using the ProtoScript II Reverse Transcriptase kit (New England BioLabs, Évry-Courcouronnes, France). qRT-PCR was performed in quadruplets on a CFX384 Touch Real-Time PCR Detection System (Biorad ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... The SUGCT insert and pcDNA5/FRT vector were ligated by using the Quick Ligation Kit (NEB) and transformed to TOP10 chemically competent cells (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were generated using NEBNext Ultra TM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA library preparation was done using NEBNext Ultra II RNA library prep kit (NEB E7770S) as per manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were then prepared using the NEBNext Ultra II directional RNA library kit (NEB).
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bioRxiv - Genomics 2023Quote: ... Library prep was performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7103) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Catalog #E7530L) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2023Quote: crRNA (Supplementary Table 2) was synthesized using a HiScribe T7 High Yield RNA Synthesis Kit (NEB). The DNA sequences includes the T7 promoter at the 5’ end and the sequence from crRNA with the target sequence at the 3’ end ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of libraries was determined using NEBNext library Quant kit for Illumina (NEB, Cat# E7630). Libraries were then pooled and sequenced by Macrogen on Ilumina Hiseq X-ten platform ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from ear punch biopsies using the Monarch Genomic DNA purification kit (NEB). Transgenes were identified using PCR primers specific for each transgene (Suppl ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by library preparation using the NEB Next Ultra II RNA Library Prep Kit (NEB E7770L). Sequencing was done using the Illumina Nextseq with 75-bp read length and single-end.
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bioRxiv - Molecular Biology 2023Quote: ... The pJC53.2_SmUbi-EGFP-SmUbi construct was assembled using the NEBuilder Hifi DNA Assembly Cloning Kit (NEB). Competent Escherichia coli DH5α (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... and the 3,240 bp fragment recovered from the gel (Monarch Gel Extraction Kit, NEB, Ipswich, MA). Components of the transgene construct ...
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bioRxiv - Molecular Biology 2023Quote: ... see below) by site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: RNA-seq libraries were prepared with NEBNext Ultra II Directional RNA-seq kit for Illumina (NEB) after rRNA depletion (NEBNext rRNA depletion kit by NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... Guide RNA were in vitro synthesized using the EnGen sgRNA Synthesis Kit (New England Biolabs, E3322V) cording to manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext rRNA Depletion Kit v2 (Human/ mouse) (NEB #E7405), NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Cancer Biology 2023Quote: ... and libraries for Illumina sequencing were prepared using the NEBNext Ultra II RNA kit (NEB, #E7770L). Quantification of the library was performed using dsDNA HS assay kit and Qubit (Molecular Probes ...
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bioRxiv - Developmental Biology 2023Quote: ... Sequencing libraries were generated using a NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, E7770S) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... mCh-Rab7A was cloned into a piggyBac plasmid using the NEB HIFI DNA Assembly Kit (NEB) and primers listed in Supp ...
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bioRxiv - Microbiology 2023Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) following the manufacturer’s directions ...
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bioRxiv - Genomics 2023Quote: ... and stranded libraries constructed using the NEBNext Ultra II directional RNA library preparation kit (NEB, E7760) following the protocol for ribosome-depleted RNA and with an 11-min RNA fragmentation step according to the manufacturer’s instructions ...
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Gene expression analyses reveal differences in children’s response to malaria according to their agebioRxiv - Microbiology 2023Quote: ... before preparation of stranded libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB). cDNA libraries were sequenced on an Illumina NovaSeq 6000 to generate ∼55-130 million paired-end reads of 75 bp per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then indexed and adaptor-ligated using NEBNEXT Ultra II DNA Library Preparation Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep kit for Illumina (NEB) combined with the NEBNext rRNA Depletion kit (NEB) ...
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bioRxiv - Genomics 2023Quote: ... and the sequencing libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The proGL2:EYFP:gl2ΔNLS construct was created using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs), taking wild-type GL2 cDNA in pENTR/D-TOPO as a template ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was then performed using Luna Universal One-step RT-qPCR kit (New England Biolabs) following the provided protocol on a BioRad CFX Opus 96 quantitative Real-Time PCR machine ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing libraries were prepared with NEBNext rRNA Depletion Kit v2 (New England Biolabs, Ipswich, MA) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... We quantified the libraries using the NEBNext Library Quant Kit for Illumina (New England Biolabs, #E7630) based on the mean insert size provided by the Bioanalyzer ...
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bioRxiv - Neuroscience 2023Quote: ... The K262E point mutation was introduced with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). pSecTag2A was used for control experiments ...
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bioRxiv - Biochemistry 2023Quote: ... the Myc/DDK tag was removed via the Q5 site-directed mutagenesis kit (New England Biolabs) using the oligonucleotides mS29 ΔMyc-DDK Forward ...
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bioRxiv - Microbiology 2023Quote: ... Prepared libraries were quantified using a NEBNext Library Quant kit for Illumina E7630L (New England BioLabs) and pooled with volumes adjusted to normalize concentrations aiming for ∼700-fold genomic coverage for population samples or ∼200-fold genomic coverage for clones ...
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bioRxiv - Genomics 2024Quote: ... a sequencing library was prepared using NEBNext® Enzymatic Methyl-seq Kit (NEB; catalog number E7120S). Library sequencing was performed using NextSeq 2000 (Illumina ...