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Citations for New England Biolabs :
4101 - 4150 of 4230 citations for R 4 5 Isopropylidene 2 pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and Gas5 shRNAs (shRNA # 1= G5C1 and shRNA # 2= G5C2) were cloned by replacing the existing Luciferase shRNA (shRLuc) under a U6 promoter using BamHI (NEB #R0136L) and EcoRI restriction sites (Table 3) ...
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bioRxiv - Microbiology 2024Quote: SARS-CoV-2 N (nucleocapsid) protein qPCR system (IDT, Iowa City, IA) and RSV N qRT-PCR (Luna, NEB, Ipswich, MA) were detected under one-step qRT-PCR on a QuantStudio 3 (Applied Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... The annealed insert (2 µl of a 1:40 dilution) was ligated into 50 ng BsaI-digested vector using T4 DNA ligase (NEB M0202). Plasmids were transformed into electrocompetent pir+ E ...
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bioRxiv - Molecular Biology 2024Quote: ... and for Figure 2 using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). Input DNA was also used as a control ...
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bioRxiv - Microbiology 2024Quote: ... plasmid pYT354 and around 2000 base pairs (2 kb) upstream and downstream the region of interest were PCR-amplified by Q5® High-Fidelity DNA Polymerase (NEB) using primers with 20 overlapping base pairs designed with Benchling ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of 10 μM uniquely barcoded universal i5 and i7 primers (Supplementary Table 2) as well as 25 μL NEBNext Ultra II Q5 2x PCR Master mix (NEB, M0544L) were added to the 21 μL DNA and a short PCR was performed (72°C for 5 min ...
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bioRxiv - Microbiology 2024Quote: Tax1bp1 was amplified by PCR from murine cDNA using the primers named Tax1bp1 fwd and rev (Table 2) and inserted by ligation-independent cloning (NEBuilder Builder HiFi DNA Assembly Cloning Kit, NEB #E5520S) into pENTR1A no ccdB (w48-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... the pHAGE lentiviral plasmid encoding SARS-CoV-2 N-LgBIT and N-SmBIT were generated by NEBuilder HiFi DNA Assembly (New England Biolabs E2621S). Oligonucleotide primers were obtained from Azenta Life Sciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Assembly reaction was performed at 1:2 vector: insert ratio using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs #E5520) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: RNA encoding indicated HSP90B1 nascent chains were prepared from PCR amplified and purified DNA template containing a T7 promoter and carried out at 37°C for 2 hours using the HiScribe® T7 High Yield RNA Synthesis Kit (New England Biolabs) and purified using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Poly(A)-tailing was performed by mixing 1 µg of DNase-treated total RNA with the following reagents: 2 µL of poly(A)-tailing buffer (NEB, #B0276SVIAL), 2 µL of 10 mM ATP (NEB ...
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bioRxiv - Genomics 2024Quote: ... First we re-amplified cDNA by combining 20 ng of the remaining cDNA from channel 2 of each sample together with 50 µl of NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs, M0541S), 2 µl of 12 µM PCR Primer1 (5’-CTACACGACGCTCTTCCGATCT-3’) ...
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bioRxiv - Biophysics 2024Quote: ... the linearized backbone and insert were mixed in a 1:2 molar ratio and ligated using Gibson assembly (NEB, Cat. # E5510S) at 50°C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... dA-tailing was performed by incubating the chromatin-bead mixture for 1 hour at 37 °C with 1× NEB Buffer 2 (NEB, B7002), 0.5 mM dATP ...
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bioRxiv - Immunology 2024Quote: ... This was followed by eight washes with NET-2 buffer and one wash with 500 μl of PNKT buffer containing 1* T4 polynucleotide kinase (PNK) buffer (NEB, M0201L) and 0.1% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... and COPG1 cDNA was PCR amplified using gene specific primers (Supplementary Table 2) and Q5® High-Fidelity DNA Polymerase (New England Biolabs). Gel purified (Macherey-Nagel ...
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bioRxiv - Developmental Biology 2024Quote: The coding sequence for the HaloTag was extracted by 2-step PCR amplification with Phusion High-Fidelity DNA Polymerase (NEB, M0530) from the pFC27A HaloTag CMV-neo Flexi vector (Promega ...
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bioRxiv - Genetics 2024Quote: ... We amplified the pgRNA cassette from the genomic DNA by performing three rounds of PCR using 500 ng of genomic DNA for each reaction and NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541S). We deep sequenced the purified libraries with paired-end sequencing ...
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bioRxiv - Genetics 2024Quote: ... we amplified the pgRNA cassette from the cloned plasmid library by three rounds of PCR with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541S). The DNA sequences of oligos used for pgRNA library cloning are listed in Supplementary Table 3 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 µl of the dissolved cells were added to a 50 µl PCR reaction (Q5 DNA polymerase, NEB; see Table S2 for primer sequences) and amplified with 36 rounds of thermal cycling ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA (400 ng) was either mock digested (i.e. without enzyme addition) or digested with BglII (New England Biolabs, 5 units/rxn, NEBuffer 3.1) for 1 hour at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 12 sRNA libraries were constructed from 5 μg of size-selected RNA using the NEBNext® Small RNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) as per the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... After addition of 200 µl nuclease elution mix (NEB nuclease P1 buffer 1 x, MgCl2 5 mM, 0.5 µl NEB nuclease P1, 0.5 µg benzonase) samples were incubated over night at 37 °C ...
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bioRxiv - Genomics 2022Quote: ... and for the synthesis of the second strand of cDNA was used the Klenow fragment 3’-5’ exo (New England Biolabs Inc., Ipswich, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 ng purified linear vector fragments were assembled with 5 µl of annealed oligonucleotide pool using NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA, USA, #E2621L) according to the manufacturer instructions ...
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bioRxiv - Physiology 2020Quote: ... pNP152 was obtained by insertion of the sta-2 promoter and sta-2 gene fused to Lifeact::mKate2 [75] into the MosSCI vector pCFJ151 [76] using Gibson Assembly (NEB Inc., MA) and confirmed by PCR or sequencing ...
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bioRxiv - Microbiology 2021Quote: ... The probes were prepared by end labelling 10 pmoles of DNA oligonucleotide complementary to the sfRNA (Supplementary Table 2) with [γ-32P]-ATP (Perkin-Elmer, USA) using T4 polynucleotide kinase (NEB, USA) and purified from unincorporated nucleotides by gel filtration on Illustra MicroSpin G-25 Columns (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Biotinylated nucleotides from the non-ligated DNA ends were removed by incubating the Hi-C libraries (2 μg) in the presence of 6 U of T4 DNA polymerase (NEB; Cat#: M0203L) in NEBuffer 2.1 supplied with 0.025 mM dATP (Thermo Fisher ...
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bioRxiv - Plant Biology 2021Quote: ... nigrum young leaf and peduncle cDNA using c70979_Fw and c70979_Rv primer and cloned into the pEAQ-HT vector(50) using NruI-HF and SmaI restriction sites and 2× Gibson Assembly master mix (NEB, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... #ltp1.4ltp1.8-1 and #ltp1.4ltp1.8-2) were chosen for constructing next generation sequencing libraries following the manufacture’s protocol (NEB Next Ultra II DNA kit). Sequencing was carried out using 2 × 150 paired-end NextSeq500 (1-2 Mio reads for all samples together ...
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bioRxiv - Biochemistry 2021Quote: ... for 2 hr at 60 °C and relinearized at the basic dSpacer furan with Ape 1 (15 U; M0282S, NEB, Ipswich, MA) for 2 hr at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The relinearized single-stranded DNA template was subjected to PCR amplification by using barcoded primers for Illumina TrueSeq small RNA sample and Phusion High-Fidelity DNA Polymerase (2 U; M0530L, NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs were subcloned into vectors through conventional ligation with Ligation high Ver.2 (Toyobo, Osaka, Japan) or NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA) according to the manufacturers’ instruction ...
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bioRxiv - Cell Biology 2020Quote: A total of 1 μg of RNA was used for cDNA library construction at Novogene using an NEBNext Ultra 2 RNA Library Prep Kit for Illumina (New England Biolabs; Cat.# E7775) as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Single primer PCR was performed in 2 different tubes for each plasmid using Q5 2X Master Mix (New England Biolabs Cat.No M0492S). After the amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... µM GAT-7N primers (5′- GTG AGT GAT GGT TGA GGT AGT GTG GAG NNN NNN N) and 2 units/µl DeepVent® (exo-) DNA polymerase (New England Biolabs, M0259L) in the programmable thermal cycler for 11 cycles ...
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bioRxiv - Microbiology 2021Quote: ... The construct was amplified by 2-step PCR according to manufacturer recommendations using Q5 high fidelity polymerase (New England Biolabs, cat#M0494S) with primers 6469TSC and 6470TSC (Supplemental table 1) ...
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bioRxiv - Microbiology 2021Quote: ... Triplicate NEBuilder HiFi assembly reactions were prepared according to manufacturer’s protocols containing ∼2:1 insert to vector as follows: 10 µl of 2X NEBuilder HiFi master mix (New England Biolabs, cat#E2621S), 158.7 ng of DNA fragments ...
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bioRxiv - Immunology 2021Quote: ... The linearized vector and the synthesized fragment in a 1:2 molar ratio were assembled with the NEBuilder HiFi DNA Assembly Kit (NEB, Ipswich, USA) at 50°C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: A total amount of 0.4 ug of RNA was used for cDNA library construction at Novogene using an NEBNext® Ultra 2 RNA Library Prep Kit for Illumina® (cat NEB #E7775 ...
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bioRxiv - Microbiology 2021Quote: ... PCRs were performed in 25 μL reaction volumes and contained 13 μL Q5® Hot Start High-Fidelity 2× Master Mix (New England Biolabs, US), 0.5 μM of each primer and 0.4 μL of 25 mg/mL BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to 150-ng DNA from each sample with or without 2 units of Phi29 polymerase (New England Biolabs, Cat# M0269L) in 20-μl total reaction volume and incubated at 30 °C for 8 hours ...
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bioRxiv - Synthetic Biology 2020Quote: ... The cDNAs were subcloned into vectors through conventional ligation with Ligation high Ver.2 (Toyobo, OSAKA, Japan) or NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions ...
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Investigations on regulation of miRNAs in rice reveal [Ca2+]cyt signal transduction regulated miRNAsbioRxiv - Plant Biology 2021Quote: Small RNA libraries were prepared with 2 μg of enriched small RNA using NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) (New England Biolabs) according to the manufacturer’s protocol in biological dupicates ...
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bioRxiv - Genomics 2021Quote: The beads were magnetically separated and 4 µl of hot PNK mix (0.2 µl PNK [New England Biolabs], 0.4 µl 32P-γ-ATP, 0.4 µl 10x PNK buffer [New England Biolabs], 3 µl H2O) was added and incubated for 5 min at 37°C in a thermomixer at 1,100 rpm ...
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bioRxiv - Genetics 2020Quote: ... and the uncut wild-type band was about 340bp and the ali1-2 mutant band was 320 bp following cleavage by the restriction endonuclease HindIII (New England Biolabs; Ipswich, MA). Digestion of the ali1-2 fragments was incomplete and as a result ...
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bioRxiv - Biophysics 2021Quote: ... was incubated with empty capsids purified as above (~2 × 1010 particles) and DNA [300 ng of 50- to 766-bp ladder DNA (New England Biolabs, Ipswich, MA) or linearized 2.8-kb or 6-kb plasmid DNA] ...
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bioRxiv - Cell Biology 2020Quote: ... TopBP1 full-length cDNA (a kind gift from Lee Zou) was amplified by PCR with primers 1 and 2 using Phusion® High-Fidelity DNA Polymerase (New England Biolabs, CM0530). The forward and reverse primers contain AscI and NotI sites ...
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bioRxiv - Microbiology 2020Quote: ... and use of a specific barcode from the NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 or 2 (New England Biolabs). Quality and quantity of total RNA ...
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bioRxiv - Immunology 2021Quote: ... Add 30 μl PCR master mix (2.5 μl Illumina dual indexes primer 1, 2.5 μl Illumina dual index primer 2, 25 μl NEB Q5 HotStart Master Mix) to the 20 μl beads bound with samples ...