Labshake search
Citations for New England Biolabs :
351 - 400 of 9421 citations for Rat Glycerol kinase GK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... 0.32 U/µL glycerol-free WarmStart Bst 2.0 (NEB #M0402B-BM), 0.3 U/µL glycerol-free WarmStart RTx (NEB #M0439B-HC1) ...
-
bioRxiv - Genetics 2021Quote: ... The forward primer was phosphorylated with T4 polynucleotide kinase (New England Biolabs) and used in a high fidelity PCR reaction (see below ...
-
bioRxiv - Genetics 2020Quote: ... 0.75 M NaCl) and 2 μl protein kinase K (NEB, Ipswich, USA) during 2 h at 60 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... Ribosome protected RNA fragments were 3’ dephosphorylated with T4 polynucleotide kinase (NEB) in 150 mM MES-NaOH pH 5.5 ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNAs were monophosphorylated on their 5’ ends using T4 polynucleotide kinase (NEB) and either 3 mM unlabeled ATP (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... Obtained RNAs were 5’-radiolabelled with T4 polynucleotide kinase (New England Biolabs) and [γ32P] adenosine triphosphate (ATP ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3’ end dephosphorylation was performed with T4 polynucleotide kinase (New England Biolabs) before addition of a pre-adenylated linker using RNA ligase (New England Biolabs) ...
-
bioRxiv - Cell Biology 2019Quote: ... the CRISPR pAS guide oligonucleotides were phosphorylated (T4 poly nucleotide kinase; NEB), annealed and ligated (Quick Ligase kit ...
-
bioRxiv - Molecular Biology 2020Quote: ... phosphorylated by T4 polynucleotide kinase at the 5’ ends (NEB, Ipswich, MA) at 37°C for 30 min ...
-
bioRxiv - Microbiology 2019Quote: ... End-labelling was done using γ32-ATP and T4 polynucleotide kinase (NEB). Radiolabelled fragments were incubated with purified proteins in buffer containing 40 mM Tris acetate pH 7.9 ...
-
bioRxiv - Molecular Biology 2019Quote: ... and the 5′-hydroxyl group was repaired with T4 polynucleotide kinase (BioLabs). The libraries were generated using TruSeq small-RNA adaptors and sequenced using NextSeq500 (Illumina).
-
bioRxiv - Microbiology 2021Quote: ... by incubating with 10 U of T4 polynucleotide kinase (New England Biolabs) at 37°C for 1 h ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5 μl of 10 U/μl T4 polynucleotide kinase (NEB; Cat#: M0201L), 1 μl of 5 U/μl Klenow DNA polymerase ...
-
bioRxiv - Synthetic Biology 2021Quote: ... the forward primers were phosphorylated using T4 polynucleotide kinase (New England Biolabs) to facilitate T4 ligation later ...
-
bioRxiv - Neuroscience 2020Quote: ... a catalytic subunit of protein kinase A (PKA Cα, New England Biolabs); Ca2+/calmodulin-dependent protein kinase II (CaMKII ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 10 units of T4 polynucleotide kinase (PNK) (New England Biolabs, #M0201S) in PNK buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Fragmented RNA was treated with 10 U of T4 polynucleotide kinase (NEB) in 20 μl reactions containing 1 mM ATP ...
-
bioRxiv - Microbiology 2021Quote: ... The two oligos were then phosphorylated using T4 polynucleotide kinase (NEB, USA) at 37°C for 1 hr ...
-
bioRxiv - Bioengineering 2020Quote: ... Probes were labeled with ATP-P32 using T4 polynucleotide kinase (NEB®) and blot was exposed to a phosphor screen (GE® ...
-
bioRxiv - Genetics 2022Quote: ... The three regional oligo pools were phosphorylated using T4 Polynucleotide Kinase (NEB) as recommended by the provider using 300 pmoles of each oligo pool and incubation for one hour at 37 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... and phosphorylated at the 5’ termini by T4 Polynucleotide Kinase (NEB, M0201L). The pBluescript plasmid was cut by EcoRV and dephosphorylated by Calf Intestinal Alkaline Phosphatase (CIP ...
-
bioRxiv - Molecular Biology 2020Quote: ... The amplicons were incubated with T4 polynucleotide kinase (New England Biolabs, USA), following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: Guides were inserted into vectors using T4 polynucleotide kinase (New England Biolabs), and the final combined guide RNA vector was assembled by Golden Gate Assembly using Quick Ligase (New England Biolabs) ...
-
bioRxiv - Biophysics 2021Quote: The NNS primers were phosphorylated with T4 polynucleotide kinase (NEB, cat#M0201S). 20 μL phosphorylations was prepared according to the following recipe ...
-
bioRxiv - Genomics 2021Quote: DNA end modification: 10 U T4 Polynucleotide Kinase (New England Biolabs, M0201) in 60 μl 1×T4 DNA Ligase reaction buffer were added to each sample and incubated at 37°C for 30 minutes.
-
bioRxiv - Cell Biology 2021Quote: ... A positive control was prepared by substituting casein kinase II(NEB, P6010S) for galectin 3 in the reaction mixture.
-
bioRxiv - Genomics 2021Quote: ... The 3’ ends were dephosphorylated with T4 polynucleotide kinase (T4 PNK; NEB) in the following reaction mix ...
-
bioRxiv - Cancer Biology 2022Quote: ... RNA samples were treated with polynucleotide kinase (New England BioLabs, Cat. #M0201) to remove the 3’-phosphate group from the uncharged tRNA followed by ligation to 5’-adenylated uniquely barcoded adapters using RNA ligase 2 truncated KQ (New England BioLabs ...
-
bioRxiv - Genomics 2022Quote: ... 6.5ul of T4 polynucleotide kinase 10U/μl (New England Biolabs cat. #M0201L) 1.3ul of Klenow polymerase 5U/μl (New England Biolabs cat ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... DNA was blunt-end repaired with T4 Polynucleotide Kinase (New England Biolabs), followed by a reaction clean-up with the MinElute purification kit (Qiagen) ...
-
bioRxiv - Genomics 2022Quote: ... specific primers (Supplementary Table S1) were radiolabelled with T4 Polynucleotide kinase (NEB) and [?-32P]ATP (6,000 Ci/mmol) ...
-
bioRxiv - Molecular Biology 2022Quote: ... prepared by labelling of oligodeoxyribonucleotide using T4 polynucleotide kinase (New England BioLabs) and ATP or [γ32P]-ATP (Perkin-Elmer ...
-
bioRxiv - Developmental Biology 2022Quote: ... RNA was then de-phosphorylated by incubating with T4 polynucleotide kinase (NEB) for 1 hr at 37° C ...
-
bioRxiv - Microbiology 2023Quote: ... by incubating with 10 U of T4 polynucleotide kinase (New England Biolabs) at 37°C for 1 h.
-
bioRxiv - Biophysics 2022Quote: ... and 10 units of T4-polynucleotide kinase (New England BioLabs, Ipswich, MA) in 70 mM Tris/HCl pH7.6 ...
-
bioRxiv - Cell Biology 2023Quote: ... Kinase reactions were performed with 1 μL commercial CK1δ (New England Biolabs) in 1x PMP buffer supplemented with 100 μM unlabeled ATP ...
-
bioRxiv - Molecular Biology 2023Quote: ... nucleic acids extracted from SPARDA were phosphorylated by T4 polynucleotyide kinase (NEB) according to the manufacturer’s protocol and were separated by 18% PAGE ...
-
bioRxiv - Molecular Biology 2023Quote: ... and end repaired with T4 Polynucleotide Kinase (T4 PNK; NEB, catalog # M0201) at 37°C for 30 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... dinucleotide primers were 5’-labeled using T4 polynucleotide kinase (New England Biolabs) and added at 20 µM without pre-annealing to the template ...
-
bioRxiv - Microbiology 2023Quote: ... 5’ phosphorylation of RNA fragments was achieved using T4 Polynucleotide Kinase (NEB) and Adenosine 5’-Triphosphate (ATP ...
-
bioRxiv - Molecular Biology 2024Quote: ... Beads were treated with 10 μl of T4 Polynucleotide kinase (PNK; NEB) in 100 μl of 1X T4 PNK Reaction buffer containing 1 U/μl SUPERase•In™ RNase Inhibitor at 37°C for 30 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... phosphorylated at 5’-termini by T4 Polynucleotide Kinase (M0201, New England Biolabs) and ligated using T4 DNA Ligase (M0202 ...
-
bioRxiv - Biochemistry 2024Quote: ... DNA substrates were 5’ terminally labelled with T4 Polynucleotide Kinase (NEB, M0201S) and ATP-γ-32P (PerkinElmer ...
-
bioRxiv - Biochemistry 2024Quote: ... The ATP and ADP makers were created using T4 polynucleotide kinase (NEB). T4 PNK mixed with 128 µM ATP supplemented with 10 nM [α-32 P]-ATP ...
-
bioRxiv - Biochemistry 2024Quote: ... 5’-end radiolabeled DNA was generated with T4 polynucleotide kinase (M0201L, NEB) and [γ-32P]ATP (NEG035C005MC ...
-
bioRxiv - Microbiology 2022Quote: ... Clean RNA was first rRNA depleted using the NEBNext rRNA depletion kit (Human/Mouse/Rat) (NEB) before being prepared for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) according to the manufacturer’s instructions with 6 µl total RNA used as input per sample ...
-
bioRxiv - Neuroscience 2020Quote: ... Mutations were generated in the rat P2X2 receptor using the Q5 Site-Directed Mutagenesis Kit (NEB). Each mutation was verified by DNA sequencing and subcloned in pWPT-EF1α-P2X2-GCaMP6s-IRES-DsRed2 or pWPT-EF1α-P2X2-GCaMP6s-P2A-mScarlet lentiviral vectors.
-
bioRxiv - Cancer Biology 2023Quote: ... library was prepared using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). The RNA-Seq libraries were sequenced on NextSeq 500 using the NextSeq 500/550 High Output Kit v2.5 (75 cycles ...
-
bioRxiv - Microbiology 2021Quote: ... and rat (New England Biolabs), following the manufactures instructions.