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Citations for New England Biolabs :
501 - 550 of 9421 citations for Rat Glycerol kinase GK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... When needed purified RNA was treated with T4 polynucleotide kinase (PNK) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2021Quote: ... When needed purified RNA was treated with T4 polynucleotide kinase (PNK) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2021Quote: ... oligonucleotide annealing was carried out in a thermocycler in T4 polynucleotide kinase reaction buffer (NEB) / 50 mM potassium acetate by heating oligonucleotide mixtures to 95 °C for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The ends of the fragmented RNA were repaired by treatment with T4 Polynucleotide Kinase (NEB) and Calf intestinal alkaline phosphatase (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... gRNA was phosphorylated by a T4 polynucleotide kinase according to product instructions (New England Biolabs). For the amplification of phosphorylated sgRNA it was incubated with 1 μl of forward and reverse primers (100 μM ...
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bioRxiv - Biophysics 2023Quote: ... the insert PCR fragment was phosphorylated with P4 polynucleotide Kinase (M0201, New England Biolabs, UK) and the destination PCR-fragment was DPN1 treated (R0176 ...
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bioRxiv - Genetics 2023Quote: ... Synthetic oligonucleotides were labeled with [γ 32P]-ATP and T4 polynucleotide kinase (New England Biolabs) at their 5’ end ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reaction was started by adding 5000 units of recombinant Casein Kinase II (NEB, P6010S) and left to incubate for 20 min at 30°C with gentle agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Sense and antisense oligos were annealed with T4 Polynucleotide Kinase (PNK) (New England Biolabs; #M0201) and ligated with the digested cutting plasmid with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... To prepare the TEV substrate two oligos were annealed using T4 Polynucleotide Kinase (NEB#M0201S) and T4 DNA ligase buffer (NEB#B0202S) ...
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bioRxiv - Molecular Biology 2023Quote: ... Small RNAs were 3’ dephosphorylated and 5’ phosphorylated with T4 polynucleotide kinase (T4PNK, NEB, #M0201S), and purified with RNA Clean and Concentrator-5 (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... The 5’ ends were radiolabelled with 20 μCi γ32P-ATP using T4 polynucleotide kinase (NEB) and separated from free nucleotides using a MicroSpin G-50 column (Cytiva) ...
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bioRxiv - Synthetic Biology 2023Quote: ... the complementary oligos were phosphorylated using T4 polynucleotide kinase from New England Biolabs (NEB, USA) first and then ligated to pVT36b using Golden Gate Assembly (Engler et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified fragments were dephosphorylated at their 3′ ends with T4 polynucleotide kinase (New England Biolabs) at 37 °C for 1 h and ligated to a 3′ adaptor sequence using T4 RNA ligase 2 (truncated K227Q ...
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bioRxiv - Molecular Biology 2023Quote: ... Probes were synthesized as ssDNA oligos and 5’ 32P-radiolabeled with T4 polynucleotide-kinase (NEB) and ψ-32P-ATP (6000 Ci/mmol ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: Seventy picomoles of oligonucleotide (40T) was labelled with polynucleotide kinase (PNK) in PNK buffer (NEB) in the presence of γ32P-ATP (Perkin-Elmer ...
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bioRxiv - Biochemistry 2023Quote: ... 50 ng of GLORI-treated RNA sample was repaired using T4 Polynucleotide Kinase (NEB, #M0201S). Pre-adenylated 3’-adapter and 5’-adapter were sequentially ligated to the RNA using T4 RNA Ligase 2 ...
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bioRxiv - Biophysics 2023Quote: ... each primer pair was annealed and consequently phosphorylated with T4-polynucleotide kinase (T4-PNK; NEB). Restriction digestion of the pLentiCRISPRv2 vector was performed using FD-Esp3I (Thermo ...
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bioRxiv - Plant Biology 2024Quote: ... dephosphorylated F4 (5 µM) was incubated with γ-[32P]-ATP and T4 polynucleotide kinase (NEB) for 45 min at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs) and self-ligated by T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... in conjunction with the NEBNext® rRNA Depletion Kit for Human/Mouse/Rat (New England BioLabs, Ipswich, MA, USA) and the MICROBExpress kit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: mRNA was enriched from 100ng DNase treated total RNA using the NEBNext rRNA depletion Kit (human, mouse, rat, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... A 140-500 ng aliquot of total RNA was rRNA depleted using NEB’s Human/Mouse/Rat RNAse-H based Depletion kit (New England BioLabs). Following rRNA removal ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
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bioRxiv - Neuroscience 2021Quote: ... The polymerase chain reaction (PCR) was performed on a genomic rat DNA template using a Taq PCR Kit (New England Biolabs), and the subsequent PCR product was purified using a Qiagen PCR Purification Kit (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Genomics 2021Quote: ... or by ribodepletion with NEBNext Human/Mouse/Rat rRNA Depletion kit followed by library construction using the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Illumina 2×151 paired end reads were generated either on the HiSeq 4000 or NovaSeq 6000 sequencing platforms (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... 500ng input RNA was treated using the NEBNext Human/Mouse/Rat rRNA Depletion kit and libraries were prepared following the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Paired end 2×151 bp reads were produced using the HiSeq 4000 platform (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... rRNA depletion was performed using a NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA was purified using Agencourt RNAClean XP Beads (New England Biolabs). Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: ... 1 μL T4 polynucleotide kinase and 1 μL DpnI (New England Biolabs, Cats. #M0201S and R0176S) and incubated at 37°C for 1 hour ...
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bioRxiv - Biophysics 2021Quote: ... Fragmented 5’-OH sites were phosphorylated by treatment with 5 units of T4 polynucleotide kinase (NEB) for 1 hour at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... 5mM DTT) and phosphorylated the 5’-ends with 25 units of T4 Polynucleotide Kinase (NEB #M0201) at 37°C for 15 minutes ...
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
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bioRxiv - Microbiology 2019Quote: ... adjacent primers containing the mutation of interest were 5′-phosphorylated using T4 polynucleotide kinase (NEB, M0201S) using the buffer from the T4 DNA ligase ...
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bioRxiv - Genetics 2019Quote: ... and phosphate groups added to the 5’ends using T4 Polynucleotide Kinase (New England Biolabs, M0201S) by first incubating for 30 minutes without ATP and then 30 min with ATP to remove and add the phosphate group ...
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bioRxiv - Microbiology 2021Quote: ... mutagenic oligonucleotides for each CDR were mixed and phosphorylated by T4 polynucleotide kinase (New England BioLabs) in 70mM Tris–HCl (pH7.6) ...
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bioRxiv - Cell Biology 2021Quote: ... The complexes on the beads were incubated with 40 units T4 Polynucleotide kinase (New England Biolabs), first with P32 labelled ATP for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Annealed oligonucleotides containing the homology region were phosphorylated with T4 polynucleotide kinase (New England Biolabs, M201) and then ligated into the Bbs1 site of the vectors pX335 and pKN7 (Appendix Tables S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1µL of each Fw and Rv were annealed and phosphorylated with T4 Polynucleotide Kinase (PNK, NEB) using the following program ...
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bioRxiv - Molecular Biology 2019Quote: ... a master mix was prepared containing 2 µl 10x T4 polynucleotide kinase buffer without ATP (NEB) and 1 µl murine RNase inhibitor per sample and 3 µl were added to each sample together with 2 µl truncated T4 polynucleotide kinase (#M0201 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein-RNA complexes were radiolabeled on-bead in 40 µl reactions with T4 polynucleotide kinase (NEB) and 2 µl [γ-32P]ATP (6,000 Ci/mmol ...
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bioRxiv - Biophysics 2020Quote: ... and were 5’ end-radiolabeled with [Ɣ-32P]-ATP using 10 U T4 polynucleotide kinase (NEB). Radiolabeled transcripts were purified on polyacrylamide gel (6% acrylamide:bisacrylamide 19:1 ...
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bioRxiv - Microbiology 2019Quote: ... phosphorylated by the T4 Polynucleotide Kinase and then incubated with T4 DNA Ligase (New England Biolabs) and transformed in E ...
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bioRxiv - Microbiology 2019Quote: ... for each sample 3 μg of DNase-digested RNA was treated with T4 Polynucleotide Kinase (NEB) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... the 3’ ends of RNA fragments were dephosphorylated with T4 polynucleotide kinase (New England BioLabs #M0201S). A preadenylated DNA adaptor sequence was ligated to the 3’-hydroxyl ends of the RNA fragments using T4 RNA Ligase (T4 RNA Ligase 2 ...
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bioRxiv - Microbiology 2021Quote: ... T4 polynucleotide kinase (T4 PNK) used in EMSAs and all other enzymes were purchased from NEB and used according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... sense and anti-sense oligo DNAs (IDT) (Table S1) were phosphorylation using T4 Polynucleotide Kinase (NEB) at 37 °C for 30 minutes ...
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bioRxiv - Genomics 2022Quote: ... denatured and phosphorylated with 10 U of 3’-phosphatase-minus T4 polynucleotides kinase (New England Biolabs) at 37 °C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... the DNA was treated in a 30 µL reaction containing 12 U T4 polynucleotide kinase (NEB), 1 mM ATP (NEB) ...
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bioRxiv - Genetics 2023Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a PhosphorImager ...