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Citations for New England Biolabs :
351 - 400 of 4752 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... the remaining 5’-phosphate groups were dephosphorylated using calf-intestinal alkaline phosphatase for 10 minutes at 37°C and finally heat inactivated for 20 min at 80°C (Quick CIP, New England Biolabs). The Rap1 arrays initially cloned in a pUC19-derived vector (Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cosmids were then digested for 2 hours at 37°C and heat-inactivated for 20 minutes at 80°C using SpeI-HF (New England Biolabs). Next ...
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bioRxiv - Biochemistry 2021Quote: ... for 2 hr at 60 °C and relinearized at the basic dSpacer furan with Ape 1 (15 U; M0282S, NEB, Ipswich, MA) for 2 hr at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR reactions were pooled and treated for 1 hr at 37°C with 25 U of Exonuclease I (NEB, Cat# M0293S) per 100 μL of pooled PCR reactions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Membranes were incubated overnight at 4 °C with the appropriate antibody: rabbit monoclonal anti-GSK3β (1:1,000; product # 9315, Cell Signalling Technology, New England BioLabs, Whitby, Ontario, Canada), rabbit polyclonal anti-p[Ser9]GSK3β (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... The heat-denatured samples were incubated at 37 °C for 1 h with 500 units of Endo H (endoglycosidase H; New England Biolabs, Ipswich, MA) in a final volume of 20 µl containing 1 × GlycoBuffer ...
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bioRxiv - Genomics 2022Quote: ... samples were gently removed from the chamber and digested overnight at 37°C in 8 U mL-1 proteinase K (NEB, cat# P8107) in digestion buffer (1× TAE buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... at 37° C for 2 hrs from 1 µg purified dsDNAs using a T7 high yield RNA synthesis kit (NEB, Cat # E2040S) in a total volume of 20 µL ...
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bioRxiv - Immunology 2021Quote: ... coli cells (NEB, catalog no C3040H, 30°C). To generate gp70 C.1086 V1V2 RQ(H/Y)173 constructs ...
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bioRxiv - Evolutionary Biology 2022Quote: ... overnight DpnI digestion at 37°C (NEB #R0176), and a second 2X ratio bead cleanup ...
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bioRxiv - Microbiology 2023Quote: ... at 37 °C or Bt.NstNBI nicking endonuclease (NEB) at 55 °C for 1 hour ...
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bioRxiv - Genetics 2023Quote: ... We finally added dual indexing primers using the i5 and i7 system from Illumina (NEB Q5 for 20 cycles, Tm 65 °C). We then performed a bead cleanup ...
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bioRxiv - Plant Biology 2021Quote: ... The immunoprecipitated protein was incubated with λ protein phosphatase using manufacturer’s protocol (Cat. # P0753S, NEB). The treated samples were resolved on SDS-PAGE and western blotting performed using OsFD7 antibody.
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bioRxiv - Plant Biology 2022Quote: ... The protein extracts were incubated with Lambda Protein Phosphatase (λ-PPase) (NEB, Cat. No. P0753S) at 30 □ for 30 min and subsequently added 5×SDS sample buffer incubated at 95 □ for 5 min ...
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CRISpy-pop: a web tool for designing CRISPR/Cas9-driven genetic modifications in diverse populationsbioRxiv - Genetics 2020Quote: The 3’ and 5’ sections of the donor DNA were first amplified individually using gradient PCR with annealing temperatures from 50°C – 70°C and Phusion® High-Fidelity DNA Polymerase (New England Biolabs). The two individual sections were then joined into the complete donor DNA fragment using the same gradient PCR protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... were heated to 65°C and slow-cooled to 37°C before reverse transcription with 5 U AMV-RT (NEB, M0277L) at 42°C for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50 ng of material was fragmented for 8 min at 37 °C and A-tailed for 30 min at 65 °C using NEBNext Ultra II FS DNA Library Prep Kit (NEB, E7805S). The fragmented and dA-tailed DNA library was ligated to dsDNA adapter having both forward (Ligation FWD primer ...
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bioRxiv - Cell Biology 2024Quote: ... were assembled in 3 separate reactions for 15 minutes at 50 °C or 55 °C using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs E2621S) alongside a control reaction omitting the insert ...
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bioRxiv - Microbiology 2021Quote: ... which were incubated with 1 μg/ml full-length S protein with His tag that had been pretreated with or without FXa (P8010L, NEB) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Dephosphorylated RNA-protein complexes were then rinsed once with NT2 buffer and 3’-end ligated with T4 RNA Ligase 1 (NEB) overnight in an Eppendorf Thermomixer at 16°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we added 150 – 1000 ng of DNA to 1 μl of prepared MBD2-Fc/protein A beads (New England Biolabs), then washed and eluted the DNA as described by Chiou and Bergey (2018) ...
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bioRxiv - Genomics 2021Quote: ... equal amount of naked DNA and DNA bound by Reb1 protein (~ 5pmol) were incubated with AAG (10 units) and APE1 (1 unit) (New England Biolabs) in a 20 μL reaction containing 1X Thermopol buffer (20mM Tris HCl pH 8.8 ...
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bioRxiv - Genomics 2022Quote: ... for 2 hours and then crosslinked with p19 siRNA binding protein (1 μg/ml in depc-PBS; New England Biolabs) or anti-N6-methyladenosine (m6A ...
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bioRxiv - Biochemistry 2023Quote: ... Cleared lysate containing the nucleosomes was incubated with purified MBP-tag or MBP-FoxP3ΔN protein (1uM) for 1 hour at 4℃ and then subjected to MBP pulldown using Amylose Resin (New England Biolabs). After proteinase K (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... TIS11B protein-RNA complexes were immunoprecipitated from 1 ml of crosslinked lysate and washed with high salt and PNK buffer (NEB). RNA was repaired by 3′ dephosphorylation and ligated to L3-IR adaptor on beads ...
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bioRxiv - Cell Biology 2023Quote: ... the reaction was stopped with a final concentration of 50 mM EDTA and the proteins were removed by treatment with proteinase K (1/100 of the volume – NEB) and SDS (0.1% ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µ l of cfDNA was incubated at 37°C for 1 hour with the following reaction mixture: NEBuffer™ 2 (NEB, B7202), 0.25 mM MnCl2 (SIGMA ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was slowly cooled down to room temperature with a Δ -1°C / second gradient and 0.5 μl of each RNase H (NEB, 5 U/μl), RNase T1 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 15 μL of cell lysate was further treated for 1 h at 37°C with 40 units of Quick calf intestinal alkaline phosphatase (CIP, New England Biolabs, Ipswich, MA, USA) per the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... In vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer (New England Biolabs Inc., Massachusetts, USA) with 1 μg RNA and 1 unit RNase Inhibitor (New England Biolabs Inc. ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA fragments encoding enriched peptide genes were mixed with sfGFP gene portion and assembled by 1 hour incubation at 50°C using HiFi Master mix (NEB Japan, Tokyo, Japan). The reaction mixture was then used in PCR amplification with primers 13 and 14 to obtain peptide-sfGFP gene fragments suitable for cell-free protein synthesis ...
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bioRxiv - Biophysics 2020Quote: Recombinant wild-type human histone H1.0 (New England Biolabs, cat. # M2501S) was used for experiments with fluorescently-labeled nucleosomes ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human CHIP was expressed in BL21(DE3) (New England Biolabs) E ...
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bioRxiv - Cell Biology 2024Quote: ... coli-derived recombinant human histone H1 (NEB, Ipswich, MA, USA #M2501), H2A (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... The cloning reaction consists of 25 cycles of 3 min at 37°C for digestion and 4 min at 16°C for ligation combining the restriction enzymes BsaI-HF (New England BioLabs, Ipswich, UK) for level 1 reaction or BpiI (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... to the RNA-probe mixture and incubated the mixture at 45°C (for Hybridase RNase H) or 37°C (for NEB RNase H) for various depletion times (10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... followed by 12 cycles of 95°C for 15 seconds and 60°C for 4 min followed by exonuclease I (New England Biolabs, PN M0293S) treatment at 37°C for 30 minutes and 80°C for 15 minutes to remove any unincorporated primers ...
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bioRxiv - Cell Biology 2020Quote: ... 1200 units λ-protein phosphatase (NEB) were diluted into phosphatase assay buffer without cell lysate ...
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bioRxiv - Microbiology 2021Quote: ... Stained and unstained protein ladders (NEB; #P7719S and #P7717S ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 units λ protein phosphatase (NEB) was added to 10 μL of the suspension ...
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bioRxiv - Molecular Biology 2020Quote: ... 1uL of lambda protein phosphatase (NEB) was added to samples ...
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bioRxiv - Bioengineering 2021Quote: ... 90 nM of SpCas9 protein (NEB), NEB buffer 3.1 (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... and Cas9 protein (NEB - 0.3 μM). This was incubated at 37°C for 10 minutes before microinjection ...
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bioRxiv - Genetics 2022Quote: ... and Cas9 protein (NEB - 0.3 μM). This was incubated at 37°C for 10 minutes before microinjection ...
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bioRxiv - Microbiology 2022Quote: ... Color Prestained Protein Standard (NEB, P7719) or Precision Plus Protein All Blue Prestained Protein Standards (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... proteins were deglycosylated by PNGaseF (NEB) prior to gel electrophoresis ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Cas9 protein was purchased from NEB EnGen Cas9 NLS ...
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bioRxiv - Microbiology 2023Quote: Protein expression vector pMAL-c5Xa (NEB) was used as the backbone for constructing the mCherry reporter ...
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bioRxiv - Biochemistry 2022Quote: ... Protein markers (New England Biolabs, #P7719S) were used for molecular mass determination.
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bioRxiv - Microbiology 2023Quote: ... proteins were deglycosylated by PNGaseF (NEB) prior to gel electrophoresis to facilitate analysis.