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Citations for New England Biolabs :
601 - 650 of 5045 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix ...
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bioRxiv - Molecular Biology 2021Quote: ... depleted with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat, NEB, Figures 6 and S6), or not depleted (Figures 2B ...
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bioRxiv - Cell Biology 2019Quote: ... Fusions to the human O6-Alkyl-DNA transferase (SNAP-tag, New England Biolabs, Beverly, MA) were expressed from plasmid pAGT-Xpress ...
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bioRxiv - Genetics 2020Quote: ... ~15nM (3ug) human genomic DNA or 30ng of gBlock in 1x Cutsmart buffer (NEB B7204) was incubated at 37°C for 15 minutes.
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bioRxiv - Cell Biology 2023Quote: Human ARHGAP18 constructs were created using Polymerase Chain Reaction (PCR) using New England Biolabs (NEB) Phusion High-Fidelity PCR Kit (catalog no ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: DNA from human cells was isolated using the Monarch®Genomic DNA Purification Kit (NEB) following the manufacturer’s instructions and including the recommended RNaseA digestion step ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: RNA from human cells was isolated using the Monarch®Total RNA Miniprep Kit (NEB) following the manufacturer’s instructions and including the on-column DnaseI digestion ...
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bioRxiv - Cancer Biology 2023Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat; NEB, E6310X). rRNA-depleted RNA was converted to a library using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... and protein samples and molecular weight markers (PageRuler Plus Pre-stained Protein Ladder, ThermoFisher Scientific 26619, or Colour pre-stained protein standards NEB P7712 or P7719) were separated using 8% ...
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bioRxiv - Cell Biology 2023Quote: ... 10 % SDS-PAGE gel was prepared and 50 μg of protein lysate was loaded along with the protein standard ladder (Cat.No.: P7719S, New England Biolabs, Ipswich, Massachusetts, United States), and electrophoresis was performed for 1.5 hr in ice-cold 1x Laemmli electrophoresis running buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Restriction digest at 37°C for 20 minutes with Msp1 (New England Biolabs) was performed to cleave the wildtype ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was digested overnight at 37°C by DpnII (New England Biolabs,. R0543M). The digestion was monitored by specific primers spanning DpnII sites in the genome (Supplemental Table 3)
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C and treated with DNAse I (New England Biolabs) at 37°C for 60 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... 5 µl was used for BglII digestion (NEB, 2 hours at 37°C) and the products were loaded on a 2% agarose gel to confirm the pAS insertion ...
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bioRxiv - Genomics 2020Quote: ... and then held at 4°C until a subsequent T7 Endonuclease I (NEB) treatment at 37°C for 30 to 90 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 μg were digested overnight at 37°C with FspEI (NEB, R0662S), which recognizes CMC sites and creates a double-stranded DNA break on the 3’ side of the modified cytosine at N12/N16 ...
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bioRxiv - Genomics 2021Quote: ... Extracted genomic DNA was digested overnight at 37°C with DpnI (NEB, R0176S) to cut adenine-methylated GATC sites ...
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bioRxiv - Cell Biology 2021Quote: ... and lysate was incubated 37°C 60 min in 2 units CIP (NEB) per 50 μL reaction containing 50μg of total protein ...
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bioRxiv - Bioengineering 2020Quote: ... IVT was performed overnight at 37°C using the HiScribe T7 kit (NEB) following the manufacturer’s instructions (N1-methyl-pseudouridine modified) ...
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bioRxiv - Microbiology 2019Quote: ... cholerae RNA at 37°C with 20 units of T4 RNA ligase (NEB) in a 20 µL reaction containing 1X T4 RNA ligase buffer ...
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bioRxiv - Cell Biology 2022Quote: ... for 10 mins at 100°C and then resuspended in EndoH (NEB, P0702S) for 1.5 hours at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... B and C oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, Cat #M0201L) according to the manufacturer recommendations ...
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bioRxiv - Genomics 2022Quote: ... ligated overnight at 16°C and subsequently transformed into competent cells (NEB C2992H).
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bioRxiv - Immunology 2022Quote: ... and C-TBGH fragments using NEBuilder HIFI DNA Assembly Mastermix (New England BioLabs). Assembled cassettes were amplified using CMV_FWD and TBGH_REV primers ...
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bioRxiv - Genomics 2023Quote: ... RCA was performed at 30°C overnight using Phi29 enzyme mix (NEB, M0269L). fluorescent probes hybridization was done at 2X SCC and 10% formamide buffer mix ...
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bioRxiv - Bioengineering 2022Quote: ... for 60 min at 37 °C and proteinase K (New England Biolabs, P8107S) for 3 hours at 55 °C ...
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bioRxiv - Microbiology 2022Quote: ... The extension products were then C-tailed using terminal transferase (New England Biolabs). The resulting C-tailed extension products were then amplified using primers CDEP4816 and P1 or CDEP4816 and P3 to generate barcoded amplicon libraries for Illumina sequencing ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli and grown at 30°C to ensure construct stability (New England Biolabs). Plasmid clones were picked and inoculated into liquid LB media for overnight culture followed by miniprep using the PureLink Plasmid MiniPrep Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: Hi-C libraries were treated with 3 U of T4 DNA polymerase (NEB) in the presence of 0.1 mM dGTP (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was phosphorylated for 1h at 37°C with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Molecular Biology 2023Quote: ... the phosphorylated ssDNA was digested overnight at 37°C with Lambda exonuclease (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Genomics 2022Quote: ... chromatin was digested overnight at 37°C with 50 U Dpn II (NEB) in each tube ...
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bioRxiv - Genetics 2023Quote: ... and then digested overnight with DpnI at 37° C (NEB; cat. no. R0176S). 3 μL of digested linear PCR product was transformed into NEB stable competent E ...
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bioRxiv - Molecular Biology 2023Quote: ... digested O/N at 42°C with 3U β-agarase (New England Biolabs) and again for 2 hrs with 2U β-agarase ...
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bioRxiv - Plant Biology 2023Quote: ... and ligated overnight at 16°C using T4 ligase (NEB, Ipswich, Massachusetts, USA). The fully reassembled BdPOX gene was ligated overnight at 16°C with the previously digested (FastDigest Eco31I ...
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bioRxiv - Systems Biology 2023Quote: ... and 72°C for 5 min) (Phusion High-Fidelity DNA Polymerase, NEB, M0530S) using the P7 and a T7-fused P5 primer (5′ TAA TAC GAC TCA CTA TAG GGA ATG ATA CGG CGA CCA CCG A 3′ ...
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bioRxiv - Cancer Biology 2023Quote: ... at 37°C for 2hrs and then treated with Proteinase K (NEB, #P8107S) at 55°C for 2 hrs ...
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bioRxiv - Molecular Biology 2023Quote: ... fully C-methylated XP12 phage DNA (Dr. Yan-Jiun Lee, NEB Ipswich MA), fully C-hydroxymethylated T414743 and fully C-hydroxy-methyl-beta-glucosylated T4 alpha-glucosyl-transferase knockout (AGT- ...
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bioRxiv - Genomics 2024Quote: ... PCR products were then digested at 37°C with DpnI (New England Biolabs) for 2 hours to remove residual starting plasmid ...
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bioRxiv - Genomics 2024Quote: ... at 65 ⁰C for 2 h followed by MspI treatment (NEB Ipswich, MA) at 37⁰C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... C-terminal GFP sequence via Gibson Assembly (New England Biolabs, Inc. Ipswich, MA). pUASTattB(pHluorin-HTTex1Q25-tdTomato ...
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bioRxiv - Molecular Biology 2021Quote: ... Analytical amounts of twenty Argonaute proteins were synthesized from pET29a plasmids using PURExpress In Vitro Protein Synthesis kit (New England Biolabs, Inc., Ipswich, MA, USA). For large scale expression and purification of CbAgo ...
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bioRxiv - Biophysics 2021Quote: ... The E1371Q samples were phosphorylated using protein kinase A (NEB). The wild-type sample was de-phosphorylated using λ-phosphatase ...
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bioRxiv - Cell Biology 2020Quote: ... 5 µl 10x buffer for Protein MetalloPhosphatases (New England Biolabs), and 5 µl 10 mM MnCl2 for 30 min at 30 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins associated to chromatin (pellet) were treated with DNaseI (NEB). Finally ...
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bioRxiv - Molecular Biology 2020Quote: ... A slurry of protein A or G magnetic beads (NEB) was used to capture enriched chromatin ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were digested using 4µl Proteinase K (800U/ml, NEB) and incubation at 55°C for 2h ...
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bioRxiv - Synthetic Biology 2019Quote: The PURExpress in vitro protein synthesis kit (New England BioLabs) was used to transcribe and translate Were-1-Fluc ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The protein was digested with PNGase F (New England BioLabs) under denaturating conditions ...
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bioRxiv - Cell Biology 2019Quote: ... The protein was purified on amylose resin beads (E8021S, NEB) according to manufacturer instructions ...