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Citations for New England Biolabs :
351 - 400 of 5202 citations for Cell Division Cycle 2 Like Protein Kinase 6 CDC2L6 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and end-labelled with T4 polynucleotide kinase (New England Biolabs). This fragment was then digested for 2 h with MfeI at 37°C in a reaction volume of 60 μl ...
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bioRxiv - Plant Biology 2023Quote: ... T4 Ligase and T4 polynucleotide kinase was obtained from NEB. Plasmids were isolated using the alkaline lysis method [15] ...
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bioRxiv - Molecular Biology 2023Quote: ... Phosphorylations and ligations were performed using T4 PNK kinase (NEB) and T4 DNA ligase (NEB) ...
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bioRxiv - Plant Biology 2023Quote: ... and 10 U of T4 Polynucleotide Kinase (New England Biolabs) in a final reaction volume of 15 μl at 37°C for 1.5 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... phosphorylated using T4 polynucleotide kinase (New England Biolabs, Ipswich, MA), and ligated into digested BsmBI-digested (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... and the primers are phosphorylated using polynucleotide kinase prior (NEB) to PCR such that each PCR product strand can be immediately ligated to itself ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5′-hydroxyl group repair (T4 polynucleotide kinase (M0201L; NEB) were conducted on beads ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 units T4 Polynucleotide Kinase (PNK, New England Biolabs, M0201S), 1x T4 PNK buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was incubated with 1 U T4 polynucleotide kinase (NEB) in presence of its buffer (70 mM tris-HCl pH 7.6 ...
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bioRxiv - Genomics 2024Quote: ... gRNAs were phosphorylated with a T4 Polynucleotide Kinase (NEB, M0201) for 30 minutes at 37°C followed by inactivation at 95°C for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... The 5’-phosphate was added via T4 polynucleotide kinase (NEB) at 37 ºC for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: Purified GST-Bbs5 protein (400 μg) was incubated with 6 μl of PKC (New England Biolabs) and 10 μl of ATP (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: pMAL-c4E vectors carrying in-frame fusions of the EFR cytoplasmic domain with the N-terminal maltose-binding protein (MBP) tag were transformed into Rosetta 2 cells (NEB) for recombinant protein expression ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μg of tissue or cell lysates were treated with or without 2 μL Lambda Protein Phosphatase (LPP) (New England Biolabs) at 30 °C for 15 min (30 min for HEK-293E lysate ...
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bioRxiv - Immunology 2023Quote: ... PCR was performed with 2 ng of library input per reaction and 7 cycles of amplification using Q5 DNA polymerase (New England Biolabs, catalog # M0491S). Libraries were quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and then PCR amplified for 20 cycles (NEB Phusion Master Mix ...
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bioRxiv - Genetics 2023Quote: ... We finally added dual indexing primers using the i5 and i7 system from Illumina (NEB Q5 for 20 cycles, Tm 65 °C). We then performed a bead cleanup ...
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bioRxiv - Molecular Biology 2022Quote: ... 2) 7 µL of Protein Deglycosylation Mix II (NEB, cat. P6044S) per slide was added for deglycosylation and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by phosphorylation of the 5’ end with T4 kinase (NEB). Phosphorylated RNA was then purified by the RNeasy kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... after which 5’end was repaired with T4 polynucleotide kinase (NEB). Nascent RNAs containing the adaptors were converted to cDNA ...
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bioRxiv - Systems Biology 2022Quote: ... and 0.5 μl of T4 polynucleotide kinase (NEB cat. no. M0201S), incubating as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... The probes were labeled using T4 polynucleotide kinase (New England BioLabs) and [γ-32P] ATP (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’ ends were phosphorylated by 20 U polynucleotide kinase (PNK; NEB) by adding 1 mM ATP (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: Ric-8A was phosphorylated using casein kinase II (New England Biolabs) as previously described10 ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 μL of T4 polynucleotide kinase (New England Biolabs, M0201S) for 45 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and then replaced with 32P using T4 polynucleotide kinase (PNK, NEB) and 32P γ-ATP (Perkin Elmer) ...
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotides were phosphorylated with T4 polynucleotide kinase (New England Biolabs #M0201L), annealed and ligated (Quick Ligation kit ...
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bioRxiv - Biophysics 2019Quote: ... first with Antarctic Phosphatase and subsequently with T4 Polinucleotide Kinase (NEB), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Variant fragments were then treated with T4 polynucleotide kinase (NEB M0201) and ligated with T4 DNA ligase (NEB M0202 ...
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bioRxiv - Genomics 2021Quote: ... 5′ end phosphorylation using T4 polynucleotide kinase (NEB, Ipswich, MA; M0201), (iv ...
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bioRxiv - Synthetic Biology 2021Quote: ... The 2810-bp PCR product was phosphorylated with polynucleotide kinase (NEB) and circularized with T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2021Quote: ... the 5′ end was phosphorylated using T4 polynucleotide kinase (NEB, M0201L), and the 5′ adapter (sequence ...
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bioRxiv - Genomics 2020Quote: ... and treated with T4 polynucleotide kinase (T4 PNK, New England Biolabs) in the presence of 1 mM ATP.
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bioRxiv - Molecular Biology 2021Quote: ... Restriction and modification (ligase, kinase, etc.) enzymes were purchased from NEB or ThermoScientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.2 μL T4 Polynucleotide Kinase (PNK) enzyme (New England BioLabs/M0201S), 1 μL 10X T4 PNK buffer and 2 μL [γ-32P] ATP (Perkin Elmer) ...
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bioRxiv - Genomics 2022Quote: ... The 5′ end was phosphorylated using T4 polynucleotide kinase (NEB, M0201L). A second bead binding was performed ...
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bioRxiv - Genomics 2022Quote: ... 5′ end phosphorylation using T4 polynucleotide kinase (NEB, Ipswich, MA; M0201), (iv ...
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bioRxiv - Molecular Biology 2023Quote: ... After PAGE purification the RNA was treated with polynucleotide kinase (NEB) to remove the residual 2’ ...
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bioRxiv - Molecular Biology 2023Quote: The kinase domain variant library was digested with PstI-HF (NEB) and NdeI-HF (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Here amplicons were incubated with T4 polynucleotide kinase (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNAs were dephospholylated by T4 polynucleotide kinase (New England Biolabs) and ligated with linker for reverse transcription ...
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bioRxiv - Molecular Biology 2023Quote: ... and 25U T4 polynucleotide kinase (10 U/μL) (New England Biolabs). The radiolabelled RNA was purified by PAGE and resuspended in 40 μL nuclease-free water ...
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bioRxiv - Biochemistry 2023Quote: Kinases were treated with 0.5 µL lambda phosphatase (New England Biolabs) per 1 µg protein for 45 min at 30°C in PMP buffer (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... The purified DNA was 5’ phosphorylated with T4 Polynucleotide Kinase (NEB) and ligated with homemade T4 DNA ligase ...
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bioRxiv - Molecular Biology 2023Quote: ... and 20 units of T4 Polynucleotide kinase (M0201L, New England Biolabs), in a total volume of 50 uL ...
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bioRxiv - Genomics 2023Quote: ... We annealed oligos in the presence of T4 Polynucleotide Kinase (NEB). We then performed Golden Gate assembly with diluted oligos ...
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bioRxiv - Bioengineering 2023Quote: Right side probes were 5’ phosphorated with T4 Polynucleotide Kinase (NEB). Probes were then column cleaned with ssDNA/RNA Clean & Concentrator (Zymo D7010 ...
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bioRxiv - Cell Biology 2023Quote: ... Klenow fragment (5 U) & T4 polynucleotide kinase (15 U) (NEB; M0201). The A-tailing was performed with Klenow exo-fragment (15 U ...
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bioRxiv - Microbiology 2023Quote: ... 25nM of DNA was radiolabeled using T4-Polynucleotide kinase (NEB M0201S) and 0.5μl of ATP(γ-32P ...
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bioRxiv - Immunology 2023Quote: ... End dephosphorylation was performed using T4 polynucleotide kinase (PNK) (NEB M0201) for 1 hour at 37°C and ligation was performed using the T4 RNA ligase I (NEB M0204 ...