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Citations for New England Biolabs :
201 - 250 of 5202 citations for Cell Division Cycle 2 Like Protein Kinase 6 CDC2L6 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... unlabeled AdoMet was left out and 6 μg of MBP2* protein (NEB) was added to each reaction to increase molecular crowding.
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bioRxiv - Synthetic Biology 2020Quote: Phosphorylate oligos by mixing 1-2 ul of each top-strand oligo along with 1x T4 ligase buffer and 1 ul T4 polynucleotide kinase (NEB). Polynucleotide kinase buffer will not work without supplementary ATP ...
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bioRxiv - Microbiology 2024Quote: ... Radioactively labelled tRNAs carrying a 2′,3′ cyclic phosphate at the 3′ end was dephosphorylated using T4 polynucleotide kinase (NEB) in 100 mM Tris-HCl pH 6.5 ...
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bioRxiv - Neuroscience 2022Quote: ... The non-15N 4R tau used for small-scale chemical in vitro crosslinking was phosphorylated using cAMP-dependent Protein Kinase (PKA, catalytic subunit, New England Biolabs #P6000S) according to manufacturer guidelines (25uL reaction at 30°C for 2 hours with 200 μM ATP ...
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bioRxiv - Cell Biology 2023Quote: Beads with bound Flag-RAD50 or GFP-BRCA1 were washed with 5M NaCl to eliminate contamination with protein kinases and incubated with 100 or 500 U of CK2 holoenzyme complex (α2/β; NEB) in 30 μl of kinase buffer (20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2022Quote: ... 100 cells were isolated directly into low protein binding eppendorfs containing 2 ul NEBNext Single Cell Lysis Buffer (NEB, E5530S). Samples were kept on dry ice until transfer to −80 C for overnight storage.
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bioRxiv - Plant Biology 2021Quote: ... transposed DNA was PCR amplified with 12 cycles using Next High-Fidelity 2×PCR Master Mix (NEB, M0541) with Nextera DNA CD Index primers ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL of Lambda Protein Phosphatase (NEB, P0753S) was added to aliquots 3 and 4 (PPase only) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 µL of λ protein phosphatase (NEB P0753S) was added and incubated at 37°C for 30 minutes and then placed on ice ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were loaded onto a 6 mL amylose column (New England Biolabs), washed in 50 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Cancer Biology 2021Quote: ... and T4 polynucleotide kinase (NEB; Cat. No. M0201L). The 3’ adenine overhangs were added to the blunt-end DNA fragments by Klenow Fragment (3’-5’ exo ...
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bioRxiv - Cell Biology 2020Quote: ... 20 units of T4 polynucleotide kinase (NEB, M0201) and water to a total volume of 20 µl ...
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bioRxiv - Genomics 2020Quote: ... 25 units of T4 polynucleotide kinase (NEB, M0201S), 2.5 units Klenow polymerase Polymerase I (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.5 U/ μl T4 Polynucleotide Kinase (NEB, M0201), 0.12 U/ μl T4 DNA Polymerase (NEB ...
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bioRxiv - Genetics 2019Quote: ... After phosphorylation (T4 Polynucleotide Kinase, New England Biolabs) and annealing by temperature ramping from 95°C to 30 s by −0.1°C/min increments ...
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bioRxiv - Molecular Biology 2020Quote: ... and a T4 polynucleotide kinase (New England Biolabs). The molecules were then circularized with T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... annealed and phosphorylated using T4 polynucleotide kinase (NEB). First sgRNA was cloned into BbsI site and second sgRNA was cloned into BsaI site ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were phosphorylated with polynucleotide kinase (NEB) and circularized with T4 DNA ligase (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... T4 Polynucleotide Kinase and DpnI (New England Biolabs), to heal nicks in the PCR products ...
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bioRxiv - Cancer Biology 2020Quote: ... and T4 polynucleotide kinase (NEB; Cat. No. M0201L). A 3’ A overhang was added to the ends of the blunted DNA fragment with Klenow Fragment (3’-5’ exo ...
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bioRxiv - Molecular Biology 2022Quote: ... and re-phosphorylation by T4 polynucletide kinase (NEB). For the oxidized sRNA library ...
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bioRxiv - Molecular Biology 2022Quote: ... 200 U/mL T4 polynucleotide Kinase (NEB, M0201), 100 U/mL DNA Polymerase I Large (Klenow ...
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bioRxiv - Microbiology 2022Quote: ... and end- labeled using T4 polynucleotide kinase (NEB) with [γ-32P] ATP (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2022Quote: ... by using T4 polynucleotide kinase (New England Biolabs) and cleaned up by using an Oligo Clean & Concentrator or RNA Clean & Concentrator Kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 units of T4 polynucleotide kinase (NEB, M0201S) and 1,200 units of Rapid T4 DNA ligase (Enzymatics ...
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bioRxiv - Biochemistry 2020Quote: ... using T4 polynucleotide kinase (PNK) (New England Biolabs). This was done by adding 1 μl 100mM MgCl2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The ONTs were phosphorylated using T4 kinase (NEB), annealed and cloned into the pSpCas9(BB)-2A-Puro backbone ...
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bioRxiv - Cell Biology 2022Quote: ... 1X T4 Polynucleotide Kinase Buffer (New England Biolabs) in a total volume of 50 µL at 37°C for one hour ...
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bioRxiv - Cell Biology 2022Quote: ... 10 units T4 polynucleotide kinase (New England Biolabs), 1X T4 Polynucleotide Kinase Buffer (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... and those from oligonucleotides using phosphonucleotide kinase (NEB) to incorporate radioactive nucleotides into the probe ...
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bioRxiv - Microbiology 2019Quote: ... 5 units T4 polynucleotide kinase (NEB cat. # M0201), 2.5 units Taq DNA polymerase (NEB cat ...
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bioRxiv - Bioengineering 2020Quote: ... and T4 Polynucleotide Kinase (New England Biolabs, M0201L). Ligation products with the correct size were purified by DNA agarose gel extraction using NucleoSpin Gel and PCR Clean-Up Kit (Takara ...
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bioRxiv - Biophysics 2021Quote: ... 5 μL of CKII kinase (New England Biolabs) and 2.5 μL λ phosphatase (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... CIP and polynucleotide kinase were purchased from NEB, Firepol Taq polymerase from Solis Biodyne ...
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bioRxiv - Evolutionary Biology 2020Quote: ... phosphorylation with T4 Polynucleotide Kinase (New England BioLabs), and circularization with an NEB Quick Ligation Kit (New England BioLabs) ...
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bioRxiv - Genetics 2020Quote: ... and T4 polynucleotide kinase (#M0201, New England Biolabs). Intramolecular circularization of the DNA was performed with T4 DNA ligase and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen #E3101K) ...
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bioRxiv - Genomics 2019Quote: ... and T4 polynucleotide kinase (NEB, Ipswich, MA, USA). Illumina adapters (containing primer sites for sequencing and flow cell surface annealing ...
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bioRxiv - Evolutionary Biology 2019Quote: ... radiolabeled using T4 Polynucleotide Kinase (New England Biolabs) and γ-32P ATP (Perkin-Elmer) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was dephosphorylated using T4 Polynucleotide Kinase (NEB). The reverse transcriptase linker was then ligated with T4 RNA Ligase 2 truncated (NEB ...
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bioRxiv - Biophysics 2022Quote: ... λ DNA was phosphorylated with T4 polynucleotide kinase (NEB), ligated to PCR-Bio/Nt.BspQI with T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2022Quote: ... with 10 U of T4 Polynucleotide kinase (NEB) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 units/µL T4 polynucleotide kinase (M0201, NEB), 0.12 units/µL T4 DNA polymerase (M0203 ...
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bioRxiv - Microbiology 2022Quote: ... containing T4 polynucleotide kinase (PNK; 0.2 μl; NEB) in a total volume of 10 μl to obtain double-stranded DNA flanked by XbaI and HindIII overhangs ...
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bioRxiv - Microbiology 2022Quote: ... The oligos were phosphorylated (T4 Polynucleotide Kinase; NEB) and annealed in a thermal cycler according to the following conditions ...
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bioRxiv - Microbiology 2022Quote: ... using the T4-polynucleotide kinase (New England Biolabs). For EMSA assays ...
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bioRxiv - Bioengineering 2023Quote: T4 Polynucleotide Kinase (NEB cat. no. M0201S/L)
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bioRxiv - Molecular Biology 2023Quote: ... dephosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to linkers by T4 RNA Ligase 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... were used together with T4 polynucleotide kinase (NEB). Biotinylated ligation products were pulled down using streptavidin beads (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 units T4 polynucleotide kinase (New England Biolabs) for 45 min at 37°C in the manufacturer’s buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... dephosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to preadenylated linkers containing the T7 promoter region by T4 RNA ligase 2 ...