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Citations for New England Biolabs :
3751 - 3800 of 5199 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... These fragments were separately inserted into the linearized pCDFDuet-1 vector using Gibson Assembly (NEB #E2611).
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bioRxiv - Cell Biology 2024Quote: ... or for 1 h in serum-free DMEM with a cocktail of heparinase I (NEB, P0735S), heparinase II (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... The product was digested by restriction enzyme PstI-HF (New England Biolabs, 37°C, 1 hour). Homozygous MET1-1 plants produced 3 digestion products (94bp ...
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bioRxiv - Molecular Biology 2024Quote: ... 1-8ng of DNA input or ChIP DNA was end-repaired with T4 DNA polymerase (NEB), DNA polymerase I ...
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bioRxiv - Molecular Biology 2024Quote: – All lanes were loaded with either Quick-Load Purple 1 Kb Plus DNA Ladder (NEB-N0550) or 1 Kb Plus DNA Ladder (ThermoFisher-10787018) ...
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bioRxiv - Biophysics 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (UDI UMI Adaptors DNA Set 1) (New England Biolabs, E7395S) with 13-19cycles of PCR ...
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bioRxiv - Biochemistry 2024Quote: ... The amidases were cloned into MCS1 of pCDFDuet-1 using Gibson assembly kit (New England Biolabs) after linearization of the vector with NcoI and HindIII (Fermentas) ...
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bioRxiv - Synthetic Biology 2024Quote: ... pH 7.5) and subjected to purification using 1 mL of Amylose Resin (New England Biolabs, Japan). The column was equilibrated with 5 column volumes (CV ...
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bioRxiv - Biophysics 2024Quote: ... the flow cell was rinsed and flushed with 50 µl of 1 mg/ml BSA (NEB) to prevent non-specific binding of the sample.
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bioRxiv - Genomics 2024Quote: ... 15 µl purified library was amplified with 1 µM NEBNext Universal PCR Primer for Illumina (NEB), 1 µM NEBNext Multiplex Oligos for Illumina (Index Primers Sets 1-4) ...
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bioRxiv - Genomics 2024Quote: ... The m4C containing genomic DNA from Natrinema pallidum BOL6-1 archaea was also obtained from NEB.
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bioRxiv - Bioengineering 2024Quote: ... necator from GenScript (Additional file 1: Table S2) and PCR amplified with Q5 High-Fidelity 2X Master Mix (NEB). The backbone plasmid pSEVA521+Pj5:GFP and the amplified insert were digested with SpeI-HF and HindIII-HF (NEB ...
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bioRxiv - Biophysics 2024Quote: ... 5 µL 10x T4 RNA ligase buffer and 5 µL T4 ssRNA ligase 1 (NEB, USA) were added and the reaction was incubated for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg was reverse-transcribed using an oligo-dT primer and ProtoScript II Reverse Transcriptase (NEB) Kit according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and A-tailed by mixing 3 µl PCR product + 1 µl 10x Thermopol buffer (NEB M0267S) + 0.2 µl ATP + 1 µl Taq polymerase and incubating the reaction at 70°C for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Gene block 1 was assembled into the digested vector with the Hifi assembly mix (NEB #E2621), and then the plasmid was transformed into NEB 5-alpha cells (C2987H) ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µL of NEBridge Golden Gate Assembly Kit (BsmBI-v2) reaction mix (New England Biolabs E1602), nuclease free water up to 10 µL ...
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bioRxiv - Cancer Biology 2024Quote: ... 9 μl of lysate was mixed to 1 μl of Glycoprotein Denaturing Buffer 10X) (NEB, B1704S). The lysate was denatured at 100°C for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C and treated with DNAse I (New England Biolabs) at 37°C for 60 minutes ...
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bioRxiv - Genomics 2020Quote: ... 4 μL of 3 U/μL NEB T4 DNA Polymerase (New England Biolabs), 1 μL of 5U/μL Klenow (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... and then held at 4°C until a subsequent T7 Endonuclease I (NEB) treatment at 37°C for 30 to 90 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 µL of labeling mix (4 µL 10X ThermoPol Reaction buffer (NEB, B9004S), 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP ...
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bioRxiv - Bioengineering 2021Quote: ... (4) lacI and tac promoter from pMAL-c5X (New England Biolabs, Ipswich, MA). The modified replacement plasmid for pMut2 ...
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bioRxiv - Physiology 2021Quote: ... Mutations in hSlo1 and β1/4 were introduced by PCR-mediated mutagenesis (NEB) using oligonucleotide primers (IDT ...
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bioRxiv - Biochemistry 2021Quote: ... 4% Glycerol and 0.1 mM DTT) with 75 µM S-Adenosylmethionine (SAM, NEB), varying amounts ssRNA and duplex RNA (see above) ...
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bioRxiv - Molecular Biology 2022Quote: ... was mixed with 7.1 μl H2O and 0.9 μl NEB 4 buffer (NEB). After incubating for 10 min at 96°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 and 4 μL of 6× Gel Loading Dye (B7025S, New England Biolabs) and 1.5 and 1 μL of 2.5 mg/mL EtBr were added ...
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bioRxiv - Genomics 2023Quote: ... The DGP-4 fragments were then cloned into the AscI/NheI (NEB, USA) site of the p200 vector to construct the sgRNA library (p200 library ...
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bioRxiv - Biochemistry 2023Quote: ... 4 U/mL creatine kinase) containing 10 µMnt cssDNA (NEB, PhiX virion DNA) was supplemented with Rad51 (5 µM ...
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bioRxiv - Cell Biology 2023Quote: ... Adapter-ligated DNA was digested with 4 µL of EcoRV-HF (NEB, R3195), incubated at 37 °C for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The gel fragments were incubated with 4 mg/mL proteinase K (NEB, P8107) in PK buffer (100 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... the product was digested by Dpn1 for 4 hours (New England Biotechnologies, NEB). The PCR product was purified by gel purification (Zymo Research) ...
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bioRxiv - Biochemistry 2024Quote: ... 4 picomoles of hybridized DNA were mixed with USER3 mix (New England Biolabs) in ThermoPol buffer (20 mM Tris-HCl pH 8.8 ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µl of T4 DNA polymerase (3,000 U/ml, NEB, cat. no. M0203S) and 1 µl of the DNA polymerase I large (Klenow ...
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bioRxiv - Microbiology 2020Quote: ... Template DNA was then digested by addition of 20 units (1 μl) DpnI restriction enzyme (NEB: R0176S) and incubation at 37°C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... RNase III cleavage reactions contained 1 mM DTT and 1.3 U of enzyme (New England Biolabs #M0245S), and were incubated for 6 min at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... 1 μL of this PCR reaction was then combined with 5 μL Q5 buffer (NEB, cat#M0491S), 0.5 μL 10mM DNTP (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... NEBNext® Multiplex Oligos for Illumina® Dual Index Primers Set 1 (cat. #E7600, New England Biolabs), and AMPure® XP Beads (cat ...
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bioRxiv - Microbiology 2021Quote: ... RT was performed using 1 μg of RNA using LunaScript™ RT SuperMix Kit (New England BioLabs) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 60 μL of each fraction was treated with 1 μL of thermolabile proteinase K (New England Biolabs) for 1 h at 37°C followed by inactivation for 10 min at 55°C.
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bioRxiv - Genomics 2020Quote: ... 1 μg of the blunt-ended DNA was further incubated in 1x T4 DNA ligase buffer (NEB) in 1x T4 DNA ligase buffer (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... The pellet was resuspended using 100μL resuspension buffer (10mM Tris pH 7.0, 1% SDS and 0.01U/μL proteinase K (NEB)) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 1μl of a 1:250 dilution of annealed gRNAs was ligated with the T4 DNA Ligase (NEB) into 36ng of the px459 V2.0 vector ...
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bioRxiv - Cell Biology 2022Quote: ... The beads to which GFP-DSB-1 had been immobilized were subject to the phosphatase assay (NEB lambda phosphatase #P0753S ...
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bioRxiv - Cell Biology 2022Quote: ... pET17b-Kif5b(1-560)-GFP-His was transformed into BL-21[DE3]RIPL cells (New England Biolabs) until an optical density at 600 nm of 0.6-0.8 and expression was induced with 0.5 mM isopropyl-β-d-thiogalactoside (IPTG ...
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bioRxiv - Genomics 2020Quote: ... 8.5 uL of this phosphorylated product was combined with 1 uL of 10X T4 ligase buffer (NEB) and 0.5 uL of T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 µg RNA was used for each reverse transcriptase reaction using M-MuLV Reverse Transcriptase (NEB) according to manufacturer protocol ...
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bioRxiv - Physiology 2020Quote: ... 1 nl of CRISPR mixture containing 2,4 μg/μl of gRNA and 0.5 μl Cas9 protein (NEB) was injected in one-cell stage embryos and raised for 24 hours ...
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bioRxiv - Biochemistry 2020Quote: ... treated with lambda phosphatase protein after purification at 30°C for 1 hr (New England BioLabs, P0753L), or co-expressed in BL21 cells with RFP-lambda phosphatase ...
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bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from 1 million cells using the Monarch Total RNA Miniprep Kit (NEB, #T2010S). We prepared cDNA from 1 μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB ...