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Citations for New England Biolabs :
3701 - 3750 of 5199 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... These were eluted and precipitated before ligation to 5’ adapters (1× T4 RNA ligase buffer (NEB), 0.5 µM p10IllLigup adapter ...
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bioRxiv - Plant Biology 2022Quote: ... We then ligated single-stranded rP5_RND adapters to 5’-ends with T4 RNA ligase 1 (NEB). Ligated RNAs were enriched and captured by oligo(dT ...
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bioRxiv - Biophysics 2022Quote: ... cells were incubated in dye solution (1 μM BG-AF647 (catalog no. S9136S, New England Biolabs), 1 mM DTT (catalog no ...
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bioRxiv - Cell Biology 2022Quote: ... Editing efficiency of bulk cell pools was initially confirmed by T7E1 (T7 Endonuclease 1, #M0302S, NEB) assay ...
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bioRxiv - Systems Biology 2023Quote: ... the flow cell was incubated with Exonuclease I mix (1 U/uL Exo I (NEB, M0293L) in 1x Exo I buffer ...
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bioRxiv - Molecular Biology 2023Quote: Endpoint PCR was performed in a 20 µL reaction volume containing 1× OneTaq Master Mix (NEB), 0.2 µM of each primer and 1 µL of 100-fold diluted cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA (1 µg) was digested with the methylation-sensitive restriction enzyme HpaII (New England Biolabs) and analyzed by Southern hybridization with a specific biotin-labeled DNA probe (300 ng ...
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bioRxiv - Microbiology 2024Quote: ... MssI and XhoI restriction enzymes and a Quick-Load® 1 kb Extend DNA Ladder (NEB). Plasmid curing was confirmed by loss of pESI related antibiotic resistance ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse-transcriptase ...
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bioRxiv - Microbiology 2024Quote: ... two inverse PCRs were carried out using R3-p21 as a template and oligonucleotides Trip.A.For/Trip.A.Rev and Trip.B.For/Trip.B.Rev (Supplementary Table 1) using Phusion High-Fidelity DNA Polymerase (New England BioLabs) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: 10µg of a plasmid containing 807bp of Pcdh10 exon 1 sequence was linearized with SacII (NEB) in 1x CutSmart buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... 5 units (2.5 μL) of RNase free DNAse I (NEW ENGLAND BioLabs, 2000 u. mL-1) and 100 μL of nuclease free water was added for every 10 μg of RNA ...
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bioRxiv - Genetics 2024Quote: ... then digested with a final concentration of 1 U/μL of DpnII (NEB cat no. R0543S) in 450 μL of 1X digestion reaction buffer ...
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bioRxiv - Biochemistry 2023Quote: ... diluted 1:10 in PBS-BSA (PBS supplemented with 0.4 mg/mL BSA (New England Biolabs)) ...
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bioRxiv - Cancer Biology 2024Quote: RNAs were transcribed from 1 µg of PCR-amplified templates using T7 RNA polymerase (NEB M0251S) for 2 h at 37 °C according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... T4 RNA ligase 1 was mixed with splinted RNA in a 1x T4 Rnl buffer (NEB) supplemented with ATP (1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant SYNJ2BP (0.5 µg) was dephosphorylated using 1 µl of calf intestinal alkaline phosphatase (CIP) (NEB) in 1x CutSmart Buffer in a total volume of 10 µl ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μl 10 pM/μl and previously adenylated using the 5′ DNA adenylation kit (NEB, E2610S), and 1 μl T4 RNA ligase 2 truncated KQ (NEB M0373L) ...
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bioRxiv - Microbiology 2023Quote: ... 200 μM dNTPs and 2500 nM NEBNext Universal and Index Primer each (Primer Set 1, NEB). PCR products were purified by native PAGE and ethanol-precipitated ...
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bioRxiv - Molecular Biology 2022Quote: The cloning procedure was as follows: 1 µl of T4 DNA Ligase (400 U; NEB M0202), 2 µl of T4 DNA Ligase Buffer (NEB M0202) ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 µl aliquots were 5’ adapter ligated (25 µl of 1× T4 RNA ligase buffer (NEB), 1 mM ATP ...
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bioRxiv - Microbiology 2023Quote: ... Pre-adenylated L3-1R-App 3’ adaptors were ligated using T4 RNA ligase 1 (M0204, NEB) for 75 min at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs, 5 U μg-1 of protein) were added with gentle mixing after each addition ...
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bioRxiv - Bioengineering 2023Quote: ... we used the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB E7600S). All sequencing was performed using an Illumina NextSeq 75 cycle high-output kit (Illumina 20024906 ...
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bioRxiv - Bioengineering 2023Quote: ... the AsCas12a-crRNA complexes were combined with 1 µL of binding buffer (New England BioLabs, #B6002S), 4.5 µL of biotinylated quencher probes ...
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bioRxiv - Genomics 2023Quote: ... and 1 uL of 10X T4 DNA ligase reaction buffer (New England Biolabs, Cat. No. B0202S) in a final volume of 10 uL ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of the HMCESSRAP-DNA crosslinking reaction and 0.5 μL of APE1 (New England BioLabs) were added to 8.5 μL final reaction buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Two micrograms of total RNA was circularized by a T4 RNA Ligase 1 Kit (M0204. NEB) and then purified by TRIzol reagent followed by isopropanol precipitation ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:100 and used for the ligation using the Quick Ligation Kit (New England Biolabs). Specific base pair substitutions were introduced with site directed mutagenesis by polymerase chain reaction (PCR) ...
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bioRxiv - Molecular Biology 2023Quote: ... The fragmented RNA was then circularized with RNA ligase 1 in 20 µl reactions (NEB, M0204S) for 2 hours at 25°C after which the circularized RNA was purified with the Oligo Clean & Concentrator kit (D4061 ...
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bioRxiv - Biochemistry 2023Quote: The PCR product was purified and 1 µg was phosphorylated using 10 units T4 PNK (NEB) in 20 µl of T4 ligase buffer (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of Snap-Cell 647 SiR (1:1000, New England Biolabs) to the media for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 µL PCR Anchor Primer,1 µL Deoxynucleotide (dNTP) Solution Mix (New England Biolabs, Inc., N0447L), 10 µL Q5® Reaction Buffer (New England BioLabs ...
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bioRxiv - Biochemistry 2023Quote: ... per 1 µg protein for 45 min at 30°C in PMP buffer (New England Biolabs) plus 1 mM MnCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for immunoblots in this study were anti-tau (tau46) (1:5000; NEB 4019S), anti-human tau (tau13 ...
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bioRxiv - Biophysics 2023Quote: ... before staining with SNAP dye buffer (1 µM BG-AF647 (catalog no. S9136S, New England Biolabs) and 1 µM dithiothreitol in 0.5% [w/v] BSA in PBS ...
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bioRxiv - Microbiology 2023Quote: ... or an equivalent mixture of each species was digested with 1 U DpnI (NEB, Ipswitch, MA) for 3 hours at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... A total of 1 μg of cDNA was prepared using the M-MulV reverse transcriptase (NEB) as per vendor instructions ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... and a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S; diluted 1:1000). The percentage of infected cells was calculated using an open-source Fiji macro (83).
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were homogenized in 1x DNA/RNA Protection reagent (New England Biolabs Inc., Part: T2011-1) and RNA was isolated using a commercially available kit (Monarch Total RNA Miniprep Kit ...
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bioRxiv - Synthetic Biology 2024Quote: ... The first cDNA synthesis was initiated by adding 1 U/µL RT at final concentration (NEB #M0277L for AMV RT ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of purified DNA template was used in the Hi-Scribe T7 transcription kit (NEB) at 37°C overnight (∼16 hr.) ...
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bioRxiv - Immunology 2024Quote: The 200 ng extracted mRNA was digested into nucleosides by Nuclease P1 (1 U, NEB, M0660S) and shrimp alkaline phosphatase (rSAP ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of total RNA was processed using NEBNext® rRNA Depletion Kit (Bacteria) (NEB, E7850). Sequencing libraries for RNA-Seq were constructed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Biophysics 2024Quote: ... 1 μg PM187B20 in a 20 μL reaction volume of 1x T4 PNK buffer (NEB, M0201S) for 60 minutes at 37 °C before heat inactivation at 65°C for 25 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 10% SDS and 3 µL of proteinase K (New England Biolabs, Ipswich, MA) were added to the suspension ...
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bioRxiv - Molecular Biology 2024Quote: ... Tat or 3’ LTR by ligation using 1 U of T4 DNA ligase (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Linearized plasmids were digested for 1 hour at 37°C by DnpI (New England Biolabs, UK) prior to performing the cloning reaction using the In-Fusion HD-Cloning Plus kit (Takara ...