Labshake search
Citations for New England Biolabs :
3651 - 3700 of 10000+ citations for Mouse Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2024Quote: ... we prepared genomic libraries with the NEBNext Ultra FS II kit (New England Biolabs) and resequenced the whole genomes at the SNP&SEQ Technology Platform in Uppsala ...
-
bioRxiv - Cell Biology 2024Quote: ... RNA was then purified using Monarch Total RNA miniprep Kit (New England Biolabs, T2010S) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... and clean-up performed using Monarch® RNA Cleanup Kit (Biolabs, New England, T2030L).
-
bioRxiv - Genetics 2024Quote: ... PCR amplifications were performed using Phusion High-Fidelity PCR kit (New England Biolabs #M0530L) according to manufacturer’s protocol using 50 ng template DNA per 100µL reaction and 18 cycles ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Recovery products were then purified with the Monarch® PCR & DNA Cleanup Kit (NEB) and eluted in 10 µl of the Monarch® DNA Elution Buffer ...
-
bioRxiv - Microbiology 2024Quote: ... cDNA was used as a template for PCR (Phusion HF kit (New England Biolabs)) with primers designed to amplify the spike and HE genes (Table 2) ...
-
bioRxiv - Immunology 2024Quote: ... transcribed in vitro using HiScribe™ T7 High Yield RNA Synthesis Kit (#E2040S, NEB) and purified with RNA Clean & Concentrator™ (#R1017 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... were transcribed and purified using HiScribe T7 High Yield Transcription Kit (New England BioLabs) and illustra Microspin G-50 Columns (GE Healthcare Life Sciences) ...
-
bioRxiv - Developmental Biology 2024Quote: ... and RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). cDNA was made using iSCRIPT reverse transcriptase (BioRad) ...
-
bioRxiv - Cell Biology 2024Quote: Prior to rRNA depletion using the NEBNext rRNA Depletion Kit v2 (New England Biolabs), 1 μg total RNA was mixed with 2 μl 1:100 diluted ERCC RNA Spike-In Mix (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2024Quote: ... samples were prepared using NEBNext UltraTMII DNA Library Prep Kit for Illumina (NEB, E7645) following manufacturers instructions ...
-
bioRxiv - Microbiology 2020Quote: ... 3 μL of in vitro transcribed 300 nM sgRNA or trL (produced as described in in vitro transcription protocol above) and 1 μL of 1 μM Cas9 nuclease (NEB, M0386S) were mixed and incubated for 10 minutes at 25°C to allow for RNP formation ...
-
bioRxiv - Biochemistry 2020Quote: ... Beads were resuspended in 1 volume of buffer A / 300 mM NaCl / 2 mM MnCl2 / 1 mM DTT and incubated with λ protein phosphatase (NEB) at 50 U / mL for 1 hour at 23°C with agitation ...
-
bioRxiv - Cell Biology 2021Quote: ... 1% NP-40, 1 mM DTT, 0.1 mg/ml cycloheximide, 1.25 mg/ml heparin, 400 U/ml NEB murine RNase inhibitor) and dounced ...
-
bioRxiv - Plant Biology 2021Quote: ... and cloned into Gateway™ pENTR™ 4 by mixing the linearized vector backbone and PCR product in a 1:1 ratio using Gibson assembly (NEB), before transformation into DH10B electro-competent E ...
-
bioRxiv - Genomics 2022Quote: ... Illumina libraries and DNA splint were mixed at a 1:1 ng ratio using NEBuilder HiFi DNA assembly Master mix (NEB #E2621). Any non-circularized DNA was digested overnight using ExoI ...
-
bioRxiv - Microbiology 2020Quote: ... The flies were homogenised in 100 μl of TE-buffer pH 8 containing 1% Triton X-100 and 1% Proteinase K (NEB, P8107S). Homogenates were incubated for 3 h at 55°C followed by a 10 min incubation step at 95°C ...
-
bioRxiv - Immunology 2022Quote: ... cleavage rates were transformed into standardized catalytic activities (1 “eqU” = equivalent to the catalytic activity of 1 U RNase HII (NEB ®) under standard conditions) ...
-
bioRxiv - Systems Biology 2019Quote: ... the Nickel-NTA beads were incubated in 80 μl 3’-linker ligation mix with (1 X PNK buffer, 1 µM 3’-adapter, 10% PEG8000, 30U Truncated T4 RNA ligase 2 K227Q (NEB, M0351L), 60U RNasin) ...
-
bioRxiv - Molecular Biology 2020Quote: ... was linked to the free hydroxyl group at the 3’-end of transcripts (1 □g of total RNA) by T4 RNA ligase 1 (NEB M0204) in the presence of 15% (w/v ...
-
bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101, 2/5 Nuclease-free water, 1/10 CutSmart Buffer 10X (NEB)] ...
-
bioRxiv - Molecular Biology 2024Quote: For proximity ligation, the pellet was resuspended into 500 μL of ligation mix (1× T4 DNA ligase buffer, 1× BSA (NEB, B9000S), 5000 CEU T4 DNA ligase (NEB ...
-
bioRxiv - Immunology 2024Quote: ... Insert and vector were combined at a 5:1 ratio and held at 50 °C for 1 hour in NEBuilder® HiFi DNA Assembly Master Mix (NEB). Gibson assembly products were purified using the MinElute® PCR Purification Kit (Qiagen) ...
-
A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... top and bottom oligonucleotides for a single R-S-R or dead end oligonucleotide pairs were combined 1 µL:1 µL and were phosphorylated in a 50 µL reaction using T4 Polynucleotide Kinase (NEB M0201L) for 2 h at 37 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were live labeled with 1 μM ATTO 590-chloroalkane and 1 μM of SNAP-Cell 647-SiR (New England Biolabs; S9102S) (final concentrations) ...
-
Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RT-PCR or qPCR was performed with 1 μl of 1:2 diluted cDNA using the Q5®Hot Start High-Fidelity 2X Master Mix (NEB) or the SYBR™ Green PCR Master Mix (ThermoFisher) ...
-
bioRxiv - Genomics 2023Quote: ... cells were finally resuspended in 400ul PSB-DTT at the concentration of ∼2e6 cells/100ul (PBS, 1% SUPERase In, 1% BSA (NEB B90000S), 1mM DTT) ...
-
bioRxiv - Genomics 2024Quote: ... of 0.1 μM polyT-rootV2 oligo diluted in hybridization buffer (2x SSC, 1 mg/mL yeast RNA, 10% dextran sulfate, 1:1000 Murine RNase inhibitor NEB M0314) on a parafilm sheet placed on a glass plate ...
-
bioRxiv - Molecular Biology 2024Quote: ... ssSynth primer was extended by adding 1 μL 10mM dNTP’s (Fisher cat#FERR0192) and 1 μL Klenow polymerase (NEB cat#M0210S) then incubating at 37°C for 1hr ...
-
bioRxiv - Cancer Biology 2020Quote: ... and murine lung slices were incubated overnight with primary antibodies in 0.1% BSA as follows: anti-CDH1 (clone EP700Y, Biozol, 1:250) or anti-CDH1-Alexa-488 (clone 24E10, New England Biolabs, 1:50), anti-Twist1 (clone Twist2C1a ...
-
bioRxiv - Microbiology 2020Quote: ... 50µl of lysed cells were transferred to a tube containing 450µL of digestion mix (1X NEB 1 buffer, 1% triton X-100). This process was repeated 11 times (total 12 tubes with 500 µl) ...
-
bioRxiv - Biochemistry 2021Quote: ... the receptor was truncated at C-terminus at two positions (i.e. 1-342 and 1-351) by inserting a STOP codon by site-directed mutagenesis (NEB, Cat. no. E0554). The surface expression of WT and truncated receptor constructs were normalized to similar levels by DNA titration in HEK-293 cells ...
-
bioRxiv - Molecular Biology 2021Quote: ... PCR products were visualized on a 1% agarose gel using the Quick-load Purple 1-Kb DNA ladder (New England Biolabs, Ipswich, MA). Primer3 Plus (Untergasser et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed in 1 mL ice-cold RNase-free cell lysis buffer supplemented with 1 μL RNase inhibitor (M0307S, NEB; Ipswich, MA). After storage in −80°C refrigerator for about 40 mins ...
-
bioRxiv - Neuroscience 2023Quote: ... then incubated with mouse anti-CaMKIIα (6G9) (1:1000, Pierce Biotechnology, Catalog #MA1-048) and rabbit anti-Gaussia luciferase (1:1000, New England BioLabs, Catalog #E8023S) overnight at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... μL supernatant were incubated with biotinylated DNA that was immobilized to streptavidin magnetic beads (1 pmol DNA to 1 μL beads; New England Biolabs, Ipswich, MA), as described in Meinhardt et al.79 ...
-
bioRxiv - Plant Biology 2019Quote: ... 1 U of enzyme (New England Biolabs, Hitchin, UK) and adjusted to final volume by adding molecular biology grade pure water ...
-
bioRxiv - Plant Biology 2019Quote: ... 1× of appropriate buffer (New England Biolabs, Hitchin, UK), 1 U of enzyme (New England Biolabs ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 U of T4-ligase (New England Biolabs). After ligation ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and 1 μl DeepVentR exo-polymerase (New England Biolabs). The PCR thermal regime consisted of 22 rounds of 94 °C for 20 sec ...
-
bioRxiv - Genomics 2020Quote: ... and 1 μl (10 U/μl) of APE1 (NEB) in a final volume of 40 μl at 37°C for 20 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 μl RtcB RNA ligase (New England Biolabs) to a total volume of 20 μl ...
-
bioRxiv - Molecular Biology 2020Quote: ... HRP conjugated (New England Biolabs, E8038S, dilution 1:10000); IRDye 680RD goat anti-mouse IgG (LI-COR ...
-
bioRxiv - Molecular Biology 2020Quote: ... using 8 units of T4 RNA ligase 1 (NEB) and 8 nmol of ATP in a final volume of 8 μl for 1 h at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were washed twice in 1× NEBuffer3.1 (NEB, B7003S) and resuspended in 342 μl of 1× NEBuffer3.1 ...
-
bioRxiv - Genomics 2022Quote: ... and Quick-Load 1 kb Extend DNA Ladder (NEB) to aid size determination ...
-
bioRxiv - Genomics 2020Quote: ... 1 μL of 5U/μL Klenow (New England Biolabs). The beads were incubated for 30 minutes at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... 1 unit/μL of DpnI (NEB; catalogue number R0176S) was added in 5 μL of purified msDNA and digested overnight at 37°C ...
-
bioRxiv - Genomics 2020Quote: ... 1 U/μl USER enzyme (both from NEB, USA), 6% v/v lymphoprep (STEMCELL Technologies ...
-
bioRxiv - Microbiology 2019Quote: ... and 1 mM of each dNTP (New England BioLabs) was aliquoted and stored at −80°C ...